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The map label for this gene is mltF [H]

Identifier: 93005941

GI number: 93005941

Start: 1341509

End: 1342717

Strand: Reverse

Name: mltF [H]

Synonym: Pcryo_1113

Alternate gene names: 93005941

Gene position: 1342717-1341509 (Counterclockwise)

Preceding gene: 93005942

Following gene: 93005937

Centisome position: 43.88

GC content: 44.33

Gene sequence:

>1209_bases
ATGCAGCGAATTACTTATCGTGCGAAAGCATCTGCGCGAGGGGCTAAACGCCGTATCTCATATATATTGCGTCCATCTAA
ACGCTTGCTAAACACCGAAAGTAACATCGTTCCAGCTATTGCATCTATCAAAGTCACTCGTTTCGCCAAAACCAAGAAAT
TGGCGCAAATCTCAAGCATCGCCCTACTTTCACTCCCTGCTGAGAGTTTGACGACCATGAATAACCAAGTACCAGCTTAT
GACCATGTTATGCTGGAGAAGACCTTGACCATCGCTGCTGTTCCAGGCGATAGCACTTATTTTGCGACCGATGGCTTCCA
ACATGGCTTTGGTTACGACTTAGTGCGTAACTATGCTGATGAGCTTGGGGTAAAAGTCACTCTCAAAGCTTATGCTGATG
AGGAAGCTGCGCTAAGAGCGTTACAGTCAGGCGATGCGGATATGGCATTGACCACGGCCAGCACCCAATTAAAAAGCCAG
CTGAATTTGTCTTCTATTAATGTCAGCTGTGGTTATGATGCCAGTTTGACCAAAAATGGCTTGCATCCTAAAGTAAGCTG
GACGTTTAATTCTGCCAATGATCCATTGTCACAAAAAGCCAGCTATTTTTTATGTGATAGCATCAAGCTTGATAACACTC
AAAAGCTAGCCGCCTTTTATAATCAAGACTTATTAAAAGATGCCTATAGTCAAGACCATTTCAAGACCACACTGACAGAA
AAGCTGCCTGATTATCAGTCTTCGTTTGAAGAACAAGCGCGCAACTACAATCATGATTGGGAGCTTTTGGTTGCCATGGG
TTATCAAGAGTCGCACTTGGATGCCAATGCAGTCTCTCCGACCGGGGTACGCGGCATTATGATGCTGACTAATAATACGG
CAAAGGCAATGGGCGTCTCAGACCGTGTTGACCCTTACCAAAGTATCGGCGGCGGTGCTCGTTACTTAGAGCAAATGAAA
ACTGATTTTGCCGATGTGCCAAAAACGGATCGTATTTGGTTTGCGCTTGCGGCTTATAATATGGGACCAAACGCTGTTAA
AGGTATTCAGCGTAAATTGAGTGCCAAAGGTATCGATGACAAAAGCTGGGCAAACATATATGCCTATCTGTCTGATAATA
AAGCGTCAAATAGCCGCTATGGTCAAGCCATGCATTATGTGAGCAATATCAGAAGCTATCTTGAAACAATAAAAACCGCC
ACGGTTTAA

Upstream 100 bases:

>100_bases
TTATAGTATTTAAAATGCACTGCTATTTATGCTGCTGATATTGGCATAAATGAGCACGTATTTTTATGGTAAGTAGGATG
GCTAAAATTAGGAGACATCC

Downstream 100 bases:

>100_bases
TCAGATATTGATAATTGATACTGCCATAAAAAAAGCTGCTCTTTTATAAGAGCAGCTTTTTTATTAACAACATTTATTCA
AACATGCACACATTAGCGAT

Product: lytic transglycosylase catalytic subunit

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MQRITYRAKASARGAKRRISYILRPSKRLLNTESNIVPAIASIKVTRFAKTKKLAQISSIALLSLPAESLTTMNNQVPAY
DHVMLEKTLTIAAVPGDSTYFATDGFQHGFGYDLVRNYADELGVKVTLKAYADEEAALRALQSGDADMALTTASTQLKSQ
LNLSSINVSCGYDASLTKNGLHPKVSWTFNSANDPLSQKASYFLCDSIKLDNTQKLAAFYNQDLLKDAYSQDHFKTTLTE
KLPDYQSSFEEQARNYNHDWELLVAMGYQESHLDANAVSPTGVRGIMMLTNNTAKAMGVSDRVDPYQSIGGGARYLEQMK
TDFADVPKTDRIWFALAAYNMGPNAVKGIQRKLSAKGIDDKSWANIYAYLSDNKASNSRYGQAMHYVSNIRSYLETIKTA
TV

Sequences:

>Translated_402_residues
MQRITYRAKASARGAKRRISYILRPSKRLLNTESNIVPAIASIKVTRFAKTKKLAQISSIALLSLPAESLTTMNNQVPAY
DHVMLEKTLTIAAVPGDSTYFATDGFQHGFGYDLVRNYADELGVKVTLKAYADEEAALRALQSGDADMALTTASTQLKSQ
LNLSSINVSCGYDASLTKNGLHPKVSWTFNSANDPLSQKASYFLCDSIKLDNTQKLAAFYNQDLLKDAYSQDHFKTTLTE
KLPDYQSSFEEQARNYNHDWELLVAMGYQESHLDANAVSPTGVRGIMMLTNNTAKAMGVSDRVDPYQSIGGGARYLEQMK
TDFADVPKTDRIWFALAAYNMGPNAVKGIQRKLSAKGIDDKSWANIYAYLSDNKASNSRYGQAMHYVSNIRSYLETIKTA
TV
>Mature_402_residues
MQRITYRAKASARGAKRRISYILRPSKRLLNTESNIVPAIASIKVTRFAKTKKLAQISSIALLSLPAESLTTMNNQVPAY
DHVMLEKTLTIAAVPGDSTYFATDGFQHGFGYDLVRNYADELGVKVTLKAYADEEAALRALQSGDADMALTTASTQLKSQ
LNLSSINVSCGYDASLTKNGLHPKVSWTFNSANDPLSQKASYFLCDSIKLDNTQKLAAFYNQDLLKDAYSQDHFKTTLTE
KLPDYQSSFEEQARNYNHDWELLVAMGYQESHLDANAVSPTGVRGIMMLTNNTAKAMGVSDRVDPYQSIGGGARYLEQMK
TDFADVPKTDRIWFALAAYNMGPNAVKGIQRKLSAKGIDDKSWANIYAYLSDNKASNSRYGQAMHYVSNIRSYLETIKTA
TV

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=164, Percent_Identity=48.1707317073171, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 44530; Mature: 44530

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRITYRAKASARGAKRRISYILRPSKRLLNTESNIVPAIASIKVTRFAKTKKLAQISSI
CCCEEEEHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHEEHHHHHHHHHHHHHHHH
ALLSLPAESLTTMNNQVPAYDHVMLEKTLTIAAVPGDSTYFATDGFQHGFGYDLVRNYAD
HEEECCHHHHHHCCCCCCCHHHHHEECEEEEEEECCCCCEEECCCCCCCCCHHHHHHHHH
ELGVKVTLKAYADEEAALRALQSGDADMALTTASTQLKSQLNLSSINVSCGYDASLTKNG
HCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHHHHCCEEEEEEECCCCCCCCCCC
LHPKVSWTFNSANDPLSQKASYFLCDSIKLDNTQKLAAFYNQDLLKDAYSQDHFKTTLTE
CCCEEEEEECCCCCCHHHCCCEEEECCEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KLPDYQSSFEEQARNYNHDWELLVAMGYQESHLDANAVSPTGVRGIMMLTNNTAKAMGVS
HCCCHHHHHHHHHHCCCCCEEEEEEECCCHHCCCCCCCCCCCCEEEEEEECCCHHHCCCC
DRVDPYQSIGGGARYLEQMKTDFADVPKTDRIWFALAAYNMGPNAVKGIQRKLSAKGIDD
CCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCC
KSWANIYAYLSDNKASNSRYGQAMHYVSNIRSYLETIKTATV
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRITYRAKASARGAKRRISYILRPSKRLLNTESNIVPAIASIKVTRFAKTKKLAQISSI
CCCEEEEHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHEEHHHHHHHHHHHHHHHH
ALLSLPAESLTTMNNQVPAYDHVMLEKTLTIAAVPGDSTYFATDGFQHGFGYDLVRNYAD
HEEECCHHHHHHCCCCCCCHHHHHEECEEEEEEECCCCCEEECCCCCCCCCHHHHHHHHH
ELGVKVTLKAYADEEAALRALQSGDADMALTTASTQLKSQLNLSSINVSCGYDASLTKNG
HCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHHHHCCEEEEEEECCCCCCCCCCC
LHPKVSWTFNSANDPLSQKASYFLCDSIKLDNTQKLAAFYNQDLLKDAYSQDHFKTTLTE
CCCEEEEEECCCCCCHHHCCCEEEECCEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KLPDYQSSFEEQARNYNHDWELLVAMGYQESHLDANAVSPTGVRGIMMLTNNTAKAMGVS
HCCCHHHHHHHHHHCCCCCEEEEEEECCCHHCCCCCCCCCCCCEEEEEEECCCHHHCCCC
DRVDPYQSIGGGARYLEQMKTDFADVPKTDRIWFALAAYNMGPNAVKGIQRKLSAKGIDD
CCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCC
KSWANIYAYLSDNKASNSRYGQAMHYVSNIRSYLETIKTATV
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA