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The map label for this gene is opuAB [H]

Identifier: 93005914

GI number: 93005914

Start: 1307671

End: 1309611

Strand: Direct

Name: opuAB [H]

Synonym: Pcryo_1086

Alternate gene names: 93005914

Gene position: 1307671-1309611 (Clockwise)

Preceding gene: 93005913

Following gene: 93005915

Centisome position: 42.74

GC content: 46.32

Gene sequence:

>1941_bases
GTGCGCCAATGGATTGCATTTAGCTTATTGCTTGTAAGCTCGCTTTTACTGTCTTTGCCAGTATCAGCTGCTGTGCCAAC
ATGCCAAGCACCGATTAAATTTGGGGCATTGACATGGGAGAGTGGTCAGTTCACAAGCGGTGTATTAAAATATATCATTG
AGAGCGGCTATGGCTGTCAGGTAGAGGAGGTGCCGGGAGCAGGTCCTGCACTTGAAACGGCATTGTCGCAAGATGATATC
CAAATCATTGGAGAGCAGTGGGTAGGGCGCTCACCCATCATGACACAAGCTATTGAGCAGAATAAAGTCGCTGTTATTGG
TGACACGCTTAAGGGCGGTGCAACTCAAGGTTGGTATGTTCCTAAATATGTACTGGACGAAAACCCGGGTCTACGCCGTT
ATCAAGATCTGCCTAAATACGCTGAGCTGTTTAAAGACCCTGAAAACCCTAGTAAATCACGCTTTATGAACTGTCCATCT
GGTTGGACGTGTGAGATTTTTAATACGCGATTGCTGAAAAATACTGGCTTGGAGAGTGATTTTAACAATGCTCATCCCGG
CACAGGCGCAGCCCTTGATGCCGAAATTGCCTCTGCTTTTGAGCAGCATAAGCCACTCTTATTTTATTACTGGCAGCCGA
CAGGTTTGATGGCAAAATACGATTTTGCCGCATTAGAATTTCCAGCCCATGATGATGCCTGCTGGCAAGACTTATTACTT
GCTGATGGCACAGCTGATTGCGTGTCTGGATTTCCTGTATCGCCTTTAGGTATCGCTGTATCTACCCCGTTTATCAATGC
CAATCCTGAGCTTGCAGATGTTTTCAAAAAAATCCAATTTAGCCCCGCTGAGCTTAATGGTGCTATTTTAGAGATGAGTG
AAAGCAAACGTAGCGGGGAGGAGCAAGCCTTTGTTTTTCTGCGTGATAACCCCCAAGTTTGGCAGCAGTGGTTATCGGAC
GAGGCAGCTACCAACCTTGCAGCTAAATTAGGTATTAGTCTAACAGGCGCTACTATCGTGACTGATAATACAGCTTCTAG
CACGAACCTAGCAGACCTATCATCTATCTTCCCTTCATGGTCACTTGAATCTCCACTGAATAACACCTTAGCCAGCGTCG
TTCGCAATCATGGCGACGTATTCCGTACGATCAGTACCGTCGCCTTAACGTATTTATTGCTTCCTATTGAGCGCCTATTA
ACGCTTATCCCACCATGGCTAATTATTGCCCTTGTGACGGTATTGGCATGGTTTGCTGTACGTAAAATTTGGTTTGCCTT
AGCATGTGGTGCTGGGTTGTTCTTGATTGGGGCATTTGGTTTATGGGGCGCATTGATTGATACCTTGGCATTGCTCATCG
TTTCAGTATGGGTAACGGTGGTTATTGGTATTCCTATCGGCATTGCCATGTCAGGAAGTAAGCTATTGCGTAAGATTGTG
ACGCCTGTATTAGATGTGATGCAAACCATGCCAAGCTTTGTTTATCTGATACCGGTACTGATGCTATTTGGTATTGGTAA
AGTGCCAGCGCTGTTTGCCACCATTATTTATGCATTACCGCCGCTTATTCGTCTGACGACCTTGGGTATTACTCAAGTGA
ATCATGAAATGGTGGAAGCAGGACGCTCGTTTGGCAGTACGCATCTGCAATTGCTTATTTGGATTAAGCTACCACAAGCG
CTGCCCAGTATCATGGCAGGGGTGAATCAAGCCGTCATGATGTCACTGTCTATGGTGGTATTGGCGTCCATGATCGGAGC
GCCAGGTCTTGGTGAAGATGTATTGCAATCTATTCAAACGCTCAATATTGGCCAAGGCTTACAAGCAGGTACGGCGATTG
TCATTGTGGCGATTATCATTGACCGCATTACTCAGGCGTTTGGGCAAGGTAAGCGTGCGCGCCAAAAAACCATTGAAGCT
GGTAGACGCCCGATTGGTTAA

Upstream 100 bases:

>100_bases
AAAATCAGTAGTAGATGATAGGCTTATTAATTGCTCAGGTAAGGCTAATGACAGCCGCTTTCATAATAAAGATAATAAAG
ATAATAAATAGGATAACGCC

Downstream 100 bases:

>100_bases
CGATTATTTGCAGACATAATAATGCCTTGTGTCGGTTTTAAAATAACTCAACTACATCAATCAATCTTAATAGATAACAA
TTCTAAAAATAAAAACAAAG

Product: glycine betaine ABC transporter substrate-binding protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MRQWIAFSLLLVSSLLLSLPVSAAVPTCQAPIKFGALTWESGQFTSGVLKYIIESGYGCQVEEVPGAGPALETALSQDDI
QIIGEQWVGRSPIMTQAIEQNKVAVIGDTLKGGATQGWYVPKYVLDENPGLRRYQDLPKYAELFKDPENPSKSRFMNCPS
GWTCEIFNTRLLKNTGLESDFNNAHPGTGAALDAEIASAFEQHKPLLFYYWQPTGLMAKYDFAALEFPAHDDACWQDLLL
ADGTADCVSGFPVSPLGIAVSTPFINANPELADVFKKIQFSPAELNGAILEMSESKRSGEEQAFVFLRDNPQVWQQWLSD
EAATNLAAKLGISLTGATIVTDNTASSTNLADLSSIFPSWSLESPLNNTLASVVRNHGDVFRTISTVALTYLLLPIERLL
TLIPPWLIIALVTVLAWFAVRKIWFALACGAGLFLIGAFGLWGALIDTLALLIVSVWVTVVIGIPIGIAMSGSKLLRKIV
TPVLDVMQTMPSFVYLIPVLMLFGIGKVPALFATIIYALPPLIRLTTLGITQVNHEMVEAGRSFGSTHLQLLIWIKLPQA
LPSIMAGVNQAVMMSLSMVVLASMIGAPGLGEDVLQSIQTLNIGQGLQAGTAIVIVAIIIDRITQAFGQGKRARQKTIEA
GRRPIG

Sequences:

>Translated_646_residues
MRQWIAFSLLLVSSLLLSLPVSAAVPTCQAPIKFGALTWESGQFTSGVLKYIIESGYGCQVEEVPGAGPALETALSQDDI
QIIGEQWVGRSPIMTQAIEQNKVAVIGDTLKGGATQGWYVPKYVLDENPGLRRYQDLPKYAELFKDPENPSKSRFMNCPS
GWTCEIFNTRLLKNTGLESDFNNAHPGTGAALDAEIASAFEQHKPLLFYYWQPTGLMAKYDFAALEFPAHDDACWQDLLL
ADGTADCVSGFPVSPLGIAVSTPFINANPELADVFKKIQFSPAELNGAILEMSESKRSGEEQAFVFLRDNPQVWQQWLSD
EAATNLAAKLGISLTGATIVTDNTASSTNLADLSSIFPSWSLESPLNNTLASVVRNHGDVFRTISTVALTYLLLPIERLL
TLIPPWLIIALVTVLAWFAVRKIWFALACGAGLFLIGAFGLWGALIDTLALLIVSVWVTVVIGIPIGIAMSGSKLLRKIV
TPVLDVMQTMPSFVYLIPVLMLFGIGKVPALFATIIYALPPLIRLTTLGITQVNHEMVEAGRSFGSTHLQLLIWIKLPQA
LPSIMAGVNQAVMMSLSMVVLASMIGAPGLGEDVLQSIQTLNIGQGLQAGTAIVIVAIIIDRITQAFGQGKRARQKTIEA
GRRPIG
>Mature_646_residues
MRQWIAFSLLLVSSLLLSLPVSAAVPTCQAPIKFGALTWESGQFTSGVLKYIIESGYGCQVEEVPGAGPALETALSQDDI
QIIGEQWVGRSPIMTQAIEQNKVAVIGDTLKGGATQGWYVPKYVLDENPGLRRYQDLPKYAELFKDPENPSKSRFMNCPS
GWTCEIFNTRLLKNTGLESDFNNAHPGTGAALDAEIASAFEQHKPLLFYYWQPTGLMAKYDFAALEFPAHDDACWQDLLL
ADGTADCVSGFPVSPLGIAVSTPFINANPELADVFKKIQFSPAELNGAILEMSESKRSGEEQAFVFLRDNPQVWQQWLSD
EAATNLAAKLGISLTGATIVTDNTASSTNLADLSSIFPSWSLESPLNNTLASVVRNHGDVFRTISTVALTYLLLPIERLL
TLIPPWLIIALVTVLAWFAVRKIWFALACGAGLFLIGAFGLWGALIDTLALLIVSVWVTVVIGIPIGIAMSGSKLLRKIV
TPVLDVMQTMPSFVYLIPVLMLFGIGKVPALFATIIYALPPLIRLTTLGITQVNHEMVEAGRSFGSTHLQLLIWIKLPQA
LPSIMAGVNQAVMMSLSMVVLASMIGAPGLGEDVLQSIQTLNIGQGLQAGTAIVIVAIIIDRITQAFGQGKRARQKTIEA
GRRPIG

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=285, Percent_Identity=43.859649122807, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788451, Length=194, Percent_Identity=30.9278350515464, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788449, Length=186, Percent_Identity=27.9569892473118, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789034, Length=268, Percent_Identity=25.7462686567164, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 69898; Mature: 69898

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50928 ABC_TM1 ; PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQWIAFSLLLVSSLLLSLPVSAAVPTCQAPIKFGALTWESGQFTSGVLKYIIESGYGCQ
CCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCE
VEEVPGAGPALETALSQDDIQIIGEQWVGRSPIMTQAIEQNKVAVIGDTLKGGATQGWYV
EECCCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCC
PKYVLDENPGLRRYQDLPKYAELFKDPENPSKSRFMNCPSGWTCEIFNTRLLKNTGLESD
CCEEECCCCCCHHHHCCHHHHHHHCCCCCCCHHHCCCCCCCCEEHHHHHHHHHHCCCCCC
FNNAHPGTGAALDAEIASAFEQHKPLLFYYWQPTGLMAKYDFAALEFPAHDDACWQDLLL
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCEEECCCCCHHHHHHHHH
ADGTADCVSGFPVSPLGIAVSTPFINANPELADVFKKIQFSPAELNGAILEMSESKRSGE
CCCCHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCHHCCCHHEEEHHHCCCCC
EQAFVFLRDNPQVWQQWLSDEAATNLAAKLGISLTGATIVTDNTASSTNLADLSSIFPSW
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCCCCCHHHHHHHCCCC
SLESPLNNTLASVVRNHGDVFRTISTVALTYLLLPIERLLTLIPPWLIIALVTVLAWFAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RKIWFALACGAGLFLIGAFGLWGALIDTLALLIVSVWVTVVIGIPIGIAMSGSKLLRKIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHH
TPVLDVMQTMPSFVYLIPVLMLFGIGKVPALFATIIYALPPLIRLTTLGITQVNHEMVEA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
GRSFGSTHLQLLIWIKLPQALPSIMAGVNQAVMMSLSMVVLASMIGAPGLGEDVLQSIQT
HHHCCCCCEEEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LNIGQGLQAGTAIVIVAIIIDRITQAFGQGKRARQKTIEAGRRPIG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRQWIAFSLLLVSSLLLSLPVSAAVPTCQAPIKFGALTWESGQFTSGVLKYIIESGYGCQ
CCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCE
VEEVPGAGPALETALSQDDIQIIGEQWVGRSPIMTQAIEQNKVAVIGDTLKGGATQGWYV
EECCCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCC
PKYVLDENPGLRRYQDLPKYAELFKDPENPSKSRFMNCPSGWTCEIFNTRLLKNTGLESD
CCEEECCCCCCHHHHCCHHHHHHHCCCCCCCHHHCCCCCCCCEEHHHHHHHHHHCCCCCC
FNNAHPGTGAALDAEIASAFEQHKPLLFYYWQPTGLMAKYDFAALEFPAHDDACWQDLLL
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCEEECCCCCHHHHHHHHH
ADGTADCVSGFPVSPLGIAVSTPFINANPELADVFKKIQFSPAELNGAILEMSESKRSGE
CCCCHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCHHCCCHHEEEHHHCCCCC
EQAFVFLRDNPQVWQQWLSDEAATNLAAKLGISLTGATIVTDNTASSTNLADLSSIFPSW
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCCCCCHHHHHHHCCCC
SLESPLNNTLASVVRNHGDVFRTISTVALTYLLLPIERLLTLIPPWLIIALVTVLAWFAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RKIWFALACGAGLFLIGAFGLWGALIDTLALLIVSVWVTVVIGIPIGIAMSGSKLLRKIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHH
TPVLDVMQTMPSFVYLIPVLMLFGIGKVPALFATIIYALPPLIRLTTLGITQVNHEMVEA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
GRSFGSTHLQLLIWIKLPQALPSIMAGVNQAVMMSLSMVVLASMIGAPGLGEDVLQSIQT
HHHCCCCCEEEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LNIGQGLQAGTAIVIVAIIIDRITQAFGQGKRARQKTIEAGRRPIG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]