Click here to switch to the map view.

The map label for this gene is dusB [H]

Identifier: 93005906

GI number: 93005906

Start: 1298836

End: 1299867

Strand: Direct

Name: dusB [H]

Synonym: Pcryo_1078

Alternate gene names: 93005906

Gene position: 1298836-1299867 (Clockwise)

Preceding gene: 93005905

Following gene: 93005907

Centisome position: 42.45

GC content: 47.0

Gene sequence:

>1032_bases
ATGTCTATTGATTCTCCAGTTTTACCTTCGTCTAATCCTACCTCAGACCACCCTTTATTGCAGCCACTCGTGATTGGCGG
CTTGACGATTGAGAACCGTTTGATAGTTGCACCGATGGCTGGCGTGACAGACAACCCTTTTCGCAGATTGTGCAAATCTT
TTGGTGCAGGTCATGCAGTCAGTGAAATGATTATCGCTGATACGGCATTGTATGCGCGCAAAAAATCGCTCTATCGTGCC
AATTTTGATAATGAAATCGCGCCAATTTCGGCACAAATTGCTGGAGCTGAACCTGATAAATTGGCAGAAGCGGCGCGTTA
TCAGATTGATAATGGTGCACAAATTATTGATATTAATATGGGCTGCCCTGCCAAAAAAGTTTGCCGTAAATTGGCAGGCT
CTGCACTATTGCAAGATGAGGATTTGGTTGCGCGCTTGCTAGATGCTGCGGTGAATGCCGTCGATGCGCCCGTTACTTTA
AAAACGCGCTTGGGCTATGAGAATGGCCGTGAAAATATTTTGCGTGTTGCAAAGCGAGCAGAGCAAGCGGGTATAGCTGC
TATTGCCATCCATGGGCGTACGCGTGAAGATATGTATACCGGAGAGGCGCGCTATGAGCTGATTCGTGAAGTCAAAGAAA
GCATCAGTATTCCAGTAATTGCCAATGGTGATATCGATAGTGCGCAAAAAGCGCAGCGTGTTTATGAGCTGACCGGCTGC
GATGCGGTGATGATTGGACGCGCTGCACAAGGACAACCATGGATATTTCGCGATATCGAGCATTTTTTGCGCACAGGTGA
GACGCTGGCATCACCCAGTGTTAGCGAGATAAAAGGAATTGTACTGACACATTTAGAAGAGCTATACGGTTTTTATGGTG
AATACTCAGGCTGCCGTATTGCTCGCAAGCACATTGCTTGGTATACCACCGGTATTCCGAACTCTAACGCCTTTAGGCAA
GCCATGTATGGTGAGGAAAGTACCGCCGGACAGTTCCGTGTGGTAGAGGATTTTTTGAATGCTCATGAATAA

Upstream 100 bases:

>100_bases
AAACTTTATGAGCTGAGCAAATGATTACGTAATCCCTTGCAATATGCGCACGAATCCTTAGAATAACGGCTTGTTTTCAT
TGCCAATCAAGCCGTTTTTT

Downstream 100 bases:

>100_bases
ACAATGAAGAGCATATGTCGAGCACAAAGTGATCCATACTTCATCTAGTAAGAATGTCTATCCAGTTAAACTGGCTTTAT
TGCTTATTTATTTCCTTTAA

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSIDSPVLPSSNPTSDHPLLQPLVIGGLTIENRLIVAPMAGVTDNPFRRLCKSFGAGHAVSEMIIADTALYARKKSLYRA
NFDNEIAPISAQIAGAEPDKLAEAARYQIDNGAQIIDINMGCPAKKVCRKLAGSALLQDEDLVARLLDAAVNAVDAPVTL
KTRLGYENGRENILRVAKRAEQAGIAAIAIHGRTREDMYTGEARYELIREVKESISIPVIANGDIDSAQKAQRVYELTGC
DAVMIGRAAQGQPWIFRDIEHFLRTGETLASPSVSEIKGIVLTHLEELYGFYGEYSGCRIARKHIAWYTTGIPNSNAFRQ
AMYGEESTAGQFRVVEDFLNAHE

Sequences:

>Translated_343_residues
MSIDSPVLPSSNPTSDHPLLQPLVIGGLTIENRLIVAPMAGVTDNPFRRLCKSFGAGHAVSEMIIADTALYARKKSLYRA
NFDNEIAPISAQIAGAEPDKLAEAARYQIDNGAQIIDINMGCPAKKVCRKLAGSALLQDEDLVARLLDAAVNAVDAPVTL
KTRLGYENGRENILRVAKRAEQAGIAAIAIHGRTREDMYTGEARYELIREVKESISIPVIANGDIDSAQKAQRVYELTGC
DAVMIGRAAQGQPWIFRDIEHFLRTGETLASPSVSEIKGIVLTHLEELYGFYGEYSGCRIARKHIAWYTTGIPNSNAFRQ
AMYGEESTAGQFRVVEDFLNAHE
>Mature_342_residues
SIDSPVLPSSNPTSDHPLLQPLVIGGLTIENRLIVAPMAGVTDNPFRRLCKSFGAGHAVSEMIIADTALYARKKSLYRAN
FDNEIAPISAQIAGAEPDKLAEAARYQIDNGAQIIDINMGCPAKKVCRKLAGSALLQDEDLVARLLDAAVNAVDAPVTLK
TRLGYENGRENILRVAKRAEQAGIAAIAIHGRTREDMYTGEARYELIREVKESISIPVIANGDIDSAQKAQRVYELTGCD
AVMIGRAAQGQPWIFRDIEHFLRTGETLASPSVSEIKGIVLTHLEELYGFYGEYSGCRIARKHIAWYTTGIPNSNAFRQA
MYGEESTAGQFRVVEDFLNAHE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=267, Percent_Identity=30.7116104868914, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI239788483, Length=241, Percent_Identity=31.1203319502075, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI8923374, Length=249, Percent_Identity=31.3253012048193, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=30.6666666666667, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI40807366, Length=247, Percent_Identity=30.3643724696356, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1789660, Length=315, Percent_Identity=50.4761904761905, Blast_Score=316, Evalue=1e-87,
Organism=Escherichia coli, GI1788462, Length=239, Percent_Identity=33.8912133891213, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI145693211, Length=315, Percent_Identity=26.3492063492064, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=252, Percent_Identity=30.5555555555556, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=264, Percent_Identity=29.9242424242424, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=259, Percent_Identity=29.7297297297297, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=220, Percent_Identity=30.9090909090909, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=28.0701754385965, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=264, Percent_Identity=29.1666666666667, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=29.004329004329, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=31.6239316239316, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580595, Length=232, Percent_Identity=32.3275862068966, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI19920448, Length=232, Percent_Identity=32.3275862068966, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI45549423, Length=268, Percent_Identity=30.9701492537313, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=28.0701754385965, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37489; Mature: 37358

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDSPVLPSSNPTSDHPLLQPLVIGGLTIENRLIVAPMAGVTDNPFRRLCKSFGAGHAV
CCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCEEEEEECCCCCCCHHHHHHHHHCCCHHH
SEMIIADTALYARKKSLYRANFDNEIAPISAQIAGAEPDKLAEAARYQIDNGAQIIDINM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHEECCCCEEEEEEC
GCPAKKVCRKLAGSALLQDEDLVARLLDAAVNAVDAPVTLKTRLGYENGRENILRVAKRA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
EQAGIAAIAIHGRTREDMYTGEARYELIREVKESISIPVIANGDIDSAQKAQRVYELTGC
HHCCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCC
DAVMIGRAAQGQPWIFRDIEHFLRTGETLASPSVSEIKGIVLTHLEELYGFYGEYSGCRI
CEEEECCCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
ARKHIAWYTTGIPNSNAFRQAMYGEESTAGQFRVVEDFLNAHE
HHHHHHEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SIDSPVLPSSNPTSDHPLLQPLVIGGLTIENRLIVAPMAGVTDNPFRRLCKSFGAGHAV
CCCCCCCCCCCCCCCCCHHHHHHHCCEEECCEEEEEECCCCCCCHHHHHHHHHCCCHHH
SEMIIADTALYARKKSLYRANFDNEIAPISAQIAGAEPDKLAEAARYQIDNGAQIIDINM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHEECCCCEEEEEEC
GCPAKKVCRKLAGSALLQDEDLVARLLDAAVNAVDAPVTLKTRLGYENGRENILRVAKRA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
EQAGIAAIAIHGRTREDMYTGEARYELIREVKESISIPVIANGDIDSAQKAQRVYELTGC
HHCCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCC
DAVMIGRAAQGQPWIFRDIEHFLRTGETLASPSVSEIKGIVLTHLEELYGFYGEYSGCRI
CEEEECCCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
ARKHIAWYTTGIPNSNAFRQAMYGEESTAGQFRVVEDFLNAHE
HHHHHHEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]