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The map label for this gene is holB [H]

Identifier: 93005893

GI number: 93005893

Start: 1281242

End: 1282360

Strand: Direct

Name: holB [H]

Synonym: Pcryo_1065

Alternate gene names: 93005893

Gene position: 1281242-1282360 (Clockwise)

Preceding gene: 93005892

Following gene: 93005894

Centisome position: 41.87

GC content: 46.83

Gene sequence:

>1119_bases
ATGAGTGAGCTGACAAATATGACAGATGATATGTATTTTGCGCCACTATTGCCATGGCAACACGAACTTTGGTCGCAGCT
GACAATGCGGGTACTAGCGCCGCAGCAATCTCTACCTCATGCGCTGTTAGCAGCTGGTATGCAAGGCATGGGCAAGCGCG
CTTTTGTATGGCGATTGGTGGCATGGTTATTATGCCGCGCGCGTGATGAGCATCCACTCGGCGCTTGCGGTCATTGTGAG
AGCTGTCAATGGCTTAGATCAGGTACGCATCCAAGCTTACAAGTGCTACCGCTAGTCAGCCTGCCAGTCAATGCTGATAG
CAGCGCTCAAAAAGAAGCGTTAGCTAATAGTACAAAAGATAAAAAATCTGCTAAGACAGCGAGTAACGCGTCATTAAAAA
TAAAGGTGGATGATATTCGTGCCTTGCAGCCTTTTATTTATCAAGGCGGGCAGGGAATGCGTATTTGTGTATTAGATCAT
GCTGAGAAAATGACCATTGCCGCCGCCAATGCGCTACTTAAAACCTTGGAAGAACCCCAAGCCCAAGTCCATTTATTTCT
GATTAGTGATGCCCCTGCGCAGCTGCTCCCAACGATTAAAAGCCGCGTTCAGCAATTGGCATTACAGACGATTGACCCTG
CTGTCGCAGTGGACTATGTGACTAAAGCATTGGGCAGCAATGTCCATCGTGAAGCAGTTGGTACAGATGCCATTGAGCAG
CTTATCCAGTTAGCAAATGGTGCGCCTTTAGCAGCTGTGGCACTGGCTCAGGCACCGTGGTATAGCAAGCGCGCGCTATG
GCTGACAACATGGCAGGCATTACGTAGCGGTAAGCGCAGTAGCGTGGCGGCAAGTGATTATTGGCAAACCCAGCTTGGTA
TTGTCGAATTTATCCAGCTGTCTGAACTCATGATAATCGATATCCGCCGTGTTGGTTTGGGCTTTGAAGCGCAGCAAAAA
GACATTAATTTTTTAATTAATTTAAATGATTATCAACCTATTGATAGTGGGTTAGAGGTGTTTGCGCATAGCTTACAACA
GACAAAAATTGCGCTGCAACAAAACGTGCAAGAAAAATTCGTCTATGATAAGCTGATGCAAGATTTGGCTTGCTTGTAA

Upstream 100 bases:

>100_bases
TAGTTTTAATTAAATTTTATAGCATTATTTTTATTCCAAATTACTCTTAGAAATTATGCTCTAACACAGATTTTTAATTG
AGAAATATTAAGTGAGTGCC

Downstream 100 bases:

>100_bases
TAGACAACAAAGCCTAAAGATGCTTTCGCATATCAGTGCATGCCACTAACTTATTATCAGTATGTATACTCTAATACTAT
ACAAGATAACCCTGTGTGAA

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSELTNMTDDMYFAPLLPWQHELWSQLTMRVLAPQQSLPHALLAAGMQGMGKRAFVWRLVAWLLCRARDEHPLGACGHCE
SCQWLRSGTHPSLQVLPLVSLPVNADSSAQKEALANSTKDKKSAKTASNASLKIKVDDIRALQPFIYQGGQGMRICVLDH
AEKMTIAAANALLKTLEEPQAQVHLFLISDAPAQLLPTIKSRVQQLALQTIDPAVAVDYVTKALGSNVHREAVGTDAIEQ
LIQLANGAPLAAVALAQAPWYSKRALWLTTWQALRSGKRSSVAASDYWQTQLGIVEFIQLSELMIIDIRRVGLGFEAQQK
DINFLINLNDYQPIDSGLEVFAHSLQQTKIALQQNVQEKFVYDKLMQDLACL

Sequences:

>Translated_372_residues
MSELTNMTDDMYFAPLLPWQHELWSQLTMRVLAPQQSLPHALLAAGMQGMGKRAFVWRLVAWLLCRARDEHPLGACGHCE
SCQWLRSGTHPSLQVLPLVSLPVNADSSAQKEALANSTKDKKSAKTASNASLKIKVDDIRALQPFIYQGGQGMRICVLDH
AEKMTIAAANALLKTLEEPQAQVHLFLISDAPAQLLPTIKSRVQQLALQTIDPAVAVDYVTKALGSNVHREAVGTDAIEQ
LIQLANGAPLAAVALAQAPWYSKRALWLTTWQALRSGKRSSVAASDYWQTQLGIVEFIQLSELMIIDIRRVGLGFEAQQK
DINFLINLNDYQPIDSGLEVFAHSLQQTKIALQQNVQEKFVYDKLMQDLACL
>Mature_371_residues
SELTNMTDDMYFAPLLPWQHELWSQLTMRVLAPQQSLPHALLAAGMQGMGKRAFVWRLVAWLLCRARDEHPLGACGHCES
CQWLRSGTHPSLQVLPLVSLPVNADSSAQKEALANSTKDKKSAKTASNASLKIKVDDIRALQPFIYQGGQGMRICVLDHA
EKMTIAAANALLKTLEEPQAQVHLFLISDAPAQLLPTIKSRVQQLALQTIDPAVAVDYVTKALGSNVHREAVGTDAIEQL
IQLANGAPLAAVALAQAPWYSKRALWLTTWQALRSGKRSSVAASDYWQTQLGIVEFIQLSELMIIDIRRVGLGFEAQQKD
INFLINLNDYQPIDSGLEVFAHSLQQTKIALQQNVQEKFVYDKLMQDLACL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41186; Mature: 41055

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTNMTDDMYFAPLLPWQHELWSQLTMRVLAPQQSLPHALLAAGMQGMGKRAFVWRLV
CCCHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHH
AWLLCRARDEHPLGACGHCESCQWLRSGTHPSLQVLPLVSLPVNADSSAQKEALANSTKD
HHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCH
KKSAKTASNASLKIKVDDIRALQPFIYQGGQGMRICVLDHAEKMTIAAANALLKTLEEPQ
HHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
AQVHLFLISDAPAQLLPTIKSRVQQLALQTIDPAVAVDYVTKALGSNVHREAVGTDAIEQ
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHCCHHHHHH
LIQLANGAPLAAVALAQAPWYSKRALWLTTWQALRSGKRSSVAASDYWQTQLGIVEFIQL
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
SELMIIDIRRVGLGFEAQQKDINFLINLNDYQPIDSGLEVFAHSLQQTKIALQQNVQEKF
HHHHEEEHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYDKLMQDLACL
HHHHHHHHHHCC
>Mature Secondary Structure 
SELTNMTDDMYFAPLLPWQHELWSQLTMRVLAPQQSLPHALLAAGMQGMGKRAFVWRLV
CCHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHH
AWLLCRARDEHPLGACGHCESCQWLRSGTHPSLQVLPLVSLPVNADSSAQKEALANSTKD
HHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCH
KKSAKTASNASLKIKVDDIRALQPFIYQGGQGMRICVLDHAEKMTIAAANALLKTLEEPQ
HHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
AQVHLFLISDAPAQLLPTIKSRVQQLALQTIDPAVAVDYVTKALGSNVHREAVGTDAIEQ
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHCCHHHHHH
LIQLANGAPLAAVALAQAPWYSKRALWLTTWQALRSGKRSSVAASDYWQTQLGIVEFIQL
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
SELMIIDIRRVGLGFEAQQKDINFLINLNDYQPIDSGLEVFAHSLQQTKIALQQNVQEKF
HHHHEEEHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYDKLMQDLACL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]