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The map label for this gene is glpK [H]

Identifier: 93005869

GI number: 93005869

Start: 1243998

End: 1245608

Strand: Direct

Name: glpK [H]

Synonym: Pcryo_1041

Alternate gene names: 93005869

Gene position: 1243998-1245608 (Clockwise)

Preceding gene: 93005864

Following gene: 93005870

Centisome position: 40.66

GC content: 46.68

Gene sequence:

>1611_bases
ATGGCAGGATATATTTTAGCGTTAGATCAAGGGACAACGTCAAGTCGAGCTATATTGTACGATGATCATGCGCGCCCAAT
CAAAATGGCACAGCAGCCAACGGTCTTAAAGACACCGCAAGCCGGTTTTGTTGAGCAAGATGCCCAGCAAATTTGGCAGA
CGCAAATCAGCTGCGCTCATGACGTCATCAATCAGGCAGGGCTACTCGCCACCGATGTTAGCAGTATCGCTATTACCAAT
CAGCGTGAATCTATTGTCATGTGGGATAAGCAAACAGGTAAGCCACTTGCCCCCGCTATTATTTGGCAAGACAGGCGTAC
AGCGAGCTACTGCGAAGCGCTTGCTGCTGAGAACCTAACCGCCACGCCACAAAGTGGTACGGTTAGTAATAATAGCCAAG
GTGATATGGCAAGCGAAGTGCAACGTATTACAGGTCTGCGTTTAGATCCATATTTCAGTGCCAGTAAAATCGCTTGGTTA
CTAGATCAAAATCCTAAATTAAGTGTACGCGCCAGCAAAGGTGAGATAGCAGTTGGCACCATAGACAGCTGGCTGATATA
TAAGCTCACAGGTGGTGAGCATGTCATCGATGTCACCAATGCTTCTCGTACCTTGCTATACGATATCCATAAGCTGGCAT
GGTCGGAGGAGTTGTGCGCCCGATTTGGCATACCCATGAATATTTTGCCCAAAGTTCTGCCATCCGATGGCGACTTTGGC
AAAACTAAAAAGGGATTATTTGCCAAACAAATCCCAATTCATGCAGTATTGGGTGACCAGCAAGCAGCACTTTTTGGTCA
AGGCTGTCTTGAGACGGGCATGGCTAAAAATACTTATGGCACTGGTTGTTTTATGCTAATGAATATCGGTCAAAAGCCGC
TGCTTAGTGAACATAAGCTACTCACAACTATTGCATGGCAACGAAAATCTGCACCAACTCGCCCAGACAATCTCTCGTTC
GAACAAATCGTCCAATCTGGCAGACGCATGCTACAGCCTCCAAGCAGAGAAGTAACTTATGCGTTAGAGGGCAGTGTTTT
TATGGCGGGTGCAATTGTACAGTGGCTGCGTGACAACCTAGGTATGTTTCAAAAAAGCGGCGAGGTAGAAGACTTGGCTC
GGCAAGTTGAGAGCAGTGAAGAGGTGGTTTTGCTGCCAGCATTTACGGGGTTAGGTGCCCCTTATTGGCGTTCAGATATC
AGTGCCAGTATTACTGGTATGAGTCGCGGTACGACCAAAGCCCATATTGCAAGGGCAGCACTCGAGGCGGTTGCTTATCA
AACATACGATGTGCTCATTGCCATGCAAAAAGACAGCCCGCATCCACTCACTGAATTAAGGGTAGATGGGGGCGCGGCAA
ACAATGATTTGTTGATGCAGTTCCAAGCAGATTTATTGAATGTGCCTGTACTGCGTCCAAGAGACACAGAGATTACTGCA
AAAGGAGCCGCTCTACTTGCTGGCTTAAAAACAGGTTTATATGATGATACAACGATGAAGGCCTCATGGCAGGTAGATCG
AGTTTTTGAGCCTAATATGTCTATAGATGTGCGTGAAAAGCATCTGTATAAATGGCAACAAGCTATCAGTAGAGCATTAA
TAGTGCCTTGA

Upstream 100 bases:

>100_bases
GGATAGCATTATCTATACTGAATTTTAGATTTTATAAATTTATAGTTAATAAATAATAATATTAGACTCAAATAAGATAA
GACAAGATGGGATGTGAGTA

Downstream 100 bases:

>100_bases
TTACTATGATAGATAGTGTTTTGCTTATTATTTAATAGCCGTTTTTCAATATTGTATAATCGACTCACCTAGAAACTACG
TCAATTGACAAAGTTTTGGT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MAGYILALDQGTTSSRAILYDDHARPIKMAQQPTVLKTPQAGFVEQDAQQIWQTQISCAHDVINQAGLLATDVSSIAITN
QRESIVMWDKQTGKPLAPAIIWQDRRTASYCEALAAENLTATPQSGTVSNNSQGDMASEVQRITGLRLDPYFSASKIAWL
LDQNPKLSVRASKGEIAVGTIDSWLIYKLTGGEHVIDVTNASRTLLYDIHKLAWSEELCARFGIPMNILPKVLPSDGDFG
KTKKGLFAKQIPIHAVLGDQQAALFGQGCLETGMAKNTYGTGCFMLMNIGQKPLLSEHKLLTTIAWQRKSAPTRPDNLSF
EQIVQSGRRMLQPPSREVTYALEGSVFMAGAIVQWLRDNLGMFQKSGEVEDLARQVESSEEVVLLPAFTGLGAPYWRSDI
SASITGMSRGTTKAHIARAALEAVAYQTYDVLIAMQKDSPHPLTELRVDGGAANNDLLMQFQADLLNVPVLRPRDTEITA
KGAALLAGLKTGLYDDTTMKASWQVDRVFEPNMSIDVREKHLYKWQQAISRALIVP

Sequences:

>Translated_536_residues
MAGYILALDQGTTSSRAILYDDHARPIKMAQQPTVLKTPQAGFVEQDAQQIWQTQISCAHDVINQAGLLATDVSSIAITN
QRESIVMWDKQTGKPLAPAIIWQDRRTASYCEALAAENLTATPQSGTVSNNSQGDMASEVQRITGLRLDPYFSASKIAWL
LDQNPKLSVRASKGEIAVGTIDSWLIYKLTGGEHVIDVTNASRTLLYDIHKLAWSEELCARFGIPMNILPKVLPSDGDFG
KTKKGLFAKQIPIHAVLGDQQAALFGQGCLETGMAKNTYGTGCFMLMNIGQKPLLSEHKLLTTIAWQRKSAPTRPDNLSF
EQIVQSGRRMLQPPSREVTYALEGSVFMAGAIVQWLRDNLGMFQKSGEVEDLARQVESSEEVVLLPAFTGLGAPYWRSDI
SASITGMSRGTTKAHIARAALEAVAYQTYDVLIAMQKDSPHPLTELRVDGGAANNDLLMQFQADLLNVPVLRPRDTEITA
KGAALLAGLKTGLYDDTTMKASWQVDRVFEPNMSIDVREKHLYKWQQAISRALIVP
>Mature_535_residues
AGYILALDQGTTSSRAILYDDHARPIKMAQQPTVLKTPQAGFVEQDAQQIWQTQISCAHDVINQAGLLATDVSSIAITNQ
RESIVMWDKQTGKPLAPAIIWQDRRTASYCEALAAENLTATPQSGTVSNNSQGDMASEVQRITGLRLDPYFSASKIAWLL
DQNPKLSVRASKGEIAVGTIDSWLIYKLTGGEHVIDVTNASRTLLYDIHKLAWSEELCARFGIPMNILPKVLPSDGDFGK
TKKGLFAKQIPIHAVLGDQQAALFGQGCLETGMAKNTYGTGCFMLMNIGQKPLLSEHKLLTTIAWQRKSAPTRPDNLSFE
QIVQSGRRMLQPPSREVTYALEGSVFMAGAIVQWLRDNLGMFQKSGEVEDLARQVESSEEVVLLPAFTGLGAPYWRSDIS
ASITGMSRGTTKAHIARAALEAVAYQTYDVLIAMQKDSPHPLTELRVDGGAANNDLLMQFQADLLNVPVLRPRDTEITAK
GAALLAGLKTGLYDDTTMKASWQVDRVFEPNMSIDVREKHLYKWQQAISRALIVP

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=537, Percent_Identity=40.5959031657356, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI4504007, Length=537, Percent_Identity=40.5959031657356, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI42794763, Length=486, Percent_Identity=42.798353909465, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI41393575, Length=537, Percent_Identity=40.9683426443203, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI88196792, Length=537, Percent_Identity=29.0502793296089, Blast_Score=256, Evalue=4e-68,
Organism=Escherichia coli, GI1790361, Length=535, Percent_Identity=44.8598130841121, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789168, Length=502, Percent_Identity=23.7051792828685, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787789, Length=554, Percent_Identity=25.0902527075812, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789987, Length=467, Percent_Identity=22.2698072805139, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=533, Percent_Identity=36.9606003752345, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI71986140, Length=554, Percent_Identity=25.2707581227437, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71986147, Length=326, Percent_Identity=28.5276073619632, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321755, Length=583, Percent_Identity=34.1337907375643, Blast_Score=276, Evalue=6e-75,
Organism=Drosophila melanogaster, GI17864214, Length=543, Percent_Identity=40.6998158379374, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24654573, Length=471, Percent_Identity=42.4628450106157, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24655555, Length=546, Percent_Identity=34.7985347985348, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24655560, Length=546, Percent_Identity=34.7985347985348, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24655570, Length=546, Percent_Identity=34.7985347985348, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24655574, Length=546, Percent_Identity=34.7985347985348, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24655565, Length=546, Percent_Identity=34.7985347985348, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI19922058, Length=540, Percent_Identity=30.5555555555556, Blast_Score=231, Evalue=9e-61,
Organism=Drosophila melanogaster, GI21357867, Length=546, Percent_Identity=29.1208791208791, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24652871, Length=536, Percent_Identity=30.2238805970149, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24656367, Length=544, Percent_Identity=29.2279411764706, Blast_Score=222, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24656372, Length=384, Percent_Identity=31.7708333333333, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI45552909, Length=357, Percent_Identity=28.2913165266106, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 58859; Mature: 58728

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGYILALDQGTTSSRAILYDDHARPIKMAQQPTVLKTPQAGFVEQDAQQIWQTQISCAH
CCCEEEEEECCCCCCEEEEEECCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
DVINQAGLLATDVSSIAITNQRESIVMWDKQTGKPLAPAIIWQDRRTASYCEALAAENLT
HHHHHCCCEEECCCCEEEECCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCC
ATPQSGTVSNNSQGDMASEVQRITGLRLDPYFSASKIAWLLDQNPKLSVRASKGEIAVGT
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEE
IDSWLIYKLTGGEHVIDVTNASRTLLYDIHKLAWSEELCARFGIPMNILPKVLPSDGDFG
CCCEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
KTKKGLFAKQIPIHAVLGDQQAALFGQGCLETGMAKNTYGTGCFMLMNIGQKPLLSEHKL
CCCCCCCHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHH
LTTIAWQRKSAPTRPDNLSFEQIVQSGRRMLQPPSREVTYALEGSVFMAGAIVQWLRDNL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
GMFQKSGEVEDLARQVESSEEVVLLPAFTGLGAPYWRSDISASITGMSRGTTKAHIARAA
HHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCEEEECCCCCCHHHHHHHHH
LEAVAYQTYDVLIAMQKDSPHPLTELRVDGGAANNDLLMQFQADLLNVPVLRPRDTEITA
HHHHHHEEEEEEEEEECCCCCCCCEEEECCCCCCCCEEEEEEHHHHCCCCCCCCCCCEEC
KGAALLAGLKTGLYDDTTMKASWQVDRVFEPNMSIDVREKHLYKWQQAISRALIVP
CHHHHHHHHHCCCCCCCCEEECEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGYILALDQGTTSSRAILYDDHARPIKMAQQPTVLKTPQAGFVEQDAQQIWQTQISCAH
CCEEEEEECCCCCCEEEEEECCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
DVINQAGLLATDVSSIAITNQRESIVMWDKQTGKPLAPAIIWQDRRTASYCEALAAENLT
HHHHHCCCEEECCCCEEEECCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCC
ATPQSGTVSNNSQGDMASEVQRITGLRLDPYFSASKIAWLLDQNPKLSVRASKGEIAVGT
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEE
IDSWLIYKLTGGEHVIDVTNASRTLLYDIHKLAWSEELCARFGIPMNILPKVLPSDGDFG
CCCEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
KTKKGLFAKQIPIHAVLGDQQAALFGQGCLETGMAKNTYGTGCFMLMNIGQKPLLSEHKL
CCCCCCCHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHH
LTTIAWQRKSAPTRPDNLSFEQIVQSGRRMLQPPSREVTYALEGSVFMAGAIVQWLRDNL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
GMFQKSGEVEDLARQVESSEEVVLLPAFTGLGAPYWRSDISASITGMSRGTTKAHIARAA
HHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCEEEECCCCCCHHHHHHHHH
LEAVAYQTYDVLIAMQKDSPHPLTELRVDGGAANNDLLMQFQADLLNVPVLRPRDTEITA
HHHHHHEEEEEEEEEECCCCCCCCEEEECCCCCCCCEEEEEEHHHHCCCCCCCCCCCEEC
KGAALLAGLKTGLYDDTTMKASWQVDRVFEPNMSIDVREKHLYKWQQAISRALIVP
CHHHHHHHHHCCCCCCCCEEECEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA