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The map label for this gene is paaF [C]

Identifier: 93005849

GI number: 93005849

Start: 1221333

End: 1222157

Strand: Reverse

Name: paaF [C]

Synonym: Pcryo_1021

Alternate gene names: 93005849

Gene position: 1222157-1221333 (Counterclockwise)

Preceding gene: 93005850

Following gene: 93005844

Centisome position: 39.94

GC content: 43.88

Gene sequence:

>825_bases
ATGACCCAATTTAACCCCATTCAATCAGTAACTCCCTCGACAAACATTAATGAGCCATTGGTTATTAGAAAAAATTCAGG
CAGTGTTGTAACGTTGACACTTAACAGTTCCAAACAATTCAATGCTTTATCTGTTAAGTTGCTAGCTGCTATGCAAGCGG
AGCTGGATAATATTGCCCAAGACAAGACCATACAAGTACTGGTGATTGCCGCAAATGGCAAGGCATTTTGTGCTGGACAT
AATCTAAAAGAGATGCGCGCCAATCCAGATGTCGCTTTTCAGCGCGCTTTATTTGAGCAATGTAGTCAGATTATGCTCAC
CATCAATCAAATGCCGCAAGTTGTCATTGCCAAAGTACAAGGCATCGCTACCGCTGCTGGTTGTCAGCTGGTCGCAGCTT
GTGATTTAGCTGTTGCCTCGGACAATGCCAAATTTGCCACCTCTGGTATCAATGTAGGGCTATTTTGTTCTACCCCTGCA
GTAGCAGTCAGTCGCAACCTATCTCGTAAGCAAGCATTTGAGATGCTTATTACTGGTGAATTTATAGATGCCCACACTGC
ACTACAGCAGGGACTGATCAATCGTGTGGCAAGCGCTGAGCAATTAGACCAGATGCTACAGTCTCTGATAGACGCTATTA
CCAATAAATCTTCAGTCGCTCTTAAAACTGGCAAAACCATGTTTTATAAGCAATTGGATATGGAATTGGCAGAGGCTTAT
GAGTATGCCAGTGAGGTGATGAGCTGCAATATGATGGCGGATGATGTGAGTGAAGGGATTGACGCCTTTATCGAAAAGCG
TTCTGCCATATGGACAGGACGATAA

Upstream 100 bases:

>100_bases
CTCTTGCTTAACCAATTAAGCATGCTACTATTTAGGTTATTATTTAAATAGCTTTTACACTTTTTCACTAAGTTTAAATA
CCCATTATCAAGGACGATAA

Downstream 100 bases:

>100_bases
GAACAACAGAGTTATTCTATAATGCAAAATGTCTCTGTTGGCGTTGGCAGCGCCACTTTTTAAATAACAAAGGCGTTTTT
TAAATAAGAAATGGCAATAA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTQFNPIQSVTPSTNINEPLVIRKNSGSVVTLTLNSSKQFNALSVKLLAAMQAELDNIAQDKTIQVLVIAANGKAFCAGH
NLKEMRANPDVAFQRALFEQCSQIMLTINQMPQVVIAKVQGIATAAGCQLVAACDLAVASDNAKFATSGINVGLFCSTPA
VAVSRNLSRKQAFEMLITGEFIDAHTALQQGLINRVASAEQLDQMLQSLIDAITNKSSVALKTGKTMFYKQLDMELAEAY
EYASEVMSCNMMADDVSEGIDAFIEKRSAIWTGR

Sequences:

>Translated_274_residues
MTQFNPIQSVTPSTNINEPLVIRKNSGSVVTLTLNSSKQFNALSVKLLAAMQAELDNIAQDKTIQVLVIAANGKAFCAGH
NLKEMRANPDVAFQRALFEQCSQIMLTINQMPQVVIAKVQGIATAAGCQLVAACDLAVASDNAKFATSGINVGLFCSTPA
VAVSRNLSRKQAFEMLITGEFIDAHTALQQGLINRVASAEQLDQMLQSLIDAITNKSSVALKTGKTMFYKQLDMELAEAY
EYASEVMSCNMMADDVSEGIDAFIEKRSAIWTGR
>Mature_273_residues
TQFNPIQSVTPSTNINEPLVIRKNSGSVVTLTLNSSKQFNALSVKLLAAMQAELDNIAQDKTIQVLVIAANGKAFCAGHN
LKEMRANPDVAFQRALFEQCSQIMLTINQMPQVVIAKVQGIATAAGCQLVAACDLAVASDNAKFATSGINVGLFCSTPAV
AVSRNLSRKQAFEMLITGEFIDAHTALQQGLINRVASAEQLDQMLQSLIDAITNKSSVALKTGKTMFYKQLDMELAEAYE
YASEVMSCNMMADDVSEGIDAFIEKRSAIWTGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=241, Percent_Identity=42.7385892116183, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=28.6852589641434, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI70995211, Length=252, Percent_Identity=28.1746031746032, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4757966, Length=245, Percent_Identity=24.8979591836735, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI51538581, Length=245, Percent_Identity=24.8979591836735, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI25453481, Length=245, Percent_Identity=24.8979591836735, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI51538573, Length=245, Percent_Identity=24.8979591836735, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=25.2100840336134, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=25.2100840336134, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=25.2100840336134, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI4757968, Length=235, Percent_Identity=24.6808510638298, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI49355787, Length=235, Percent_Identity=24.6808510638298, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=31.4176245210728, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=30.7116104868914, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=24.1379310344828, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=26.7716535433071, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=25.296442687747, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=40.1639344262295, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=29.0836653386454, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=25.7936507936508, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=23.6947791164659, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=244, Percent_Identity=25.8196721311475, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=233, Percent_Identity=26.1802575107296, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=24.7011952191235, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=235, Percent_Identity=23.4042553191489, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=210, Percent_Identity=22.3809523809524, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=38.6363636363636, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI20129971, Length=275, Percent_Identity=30.5454545454545, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653477, Length=275, Percent_Identity=30.5454545454545, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653139, Length=276, Percent_Identity=25.7246376811594, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19920382, Length=308, Percent_Identity=22.4025974025974, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29578; Mature: 29447

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFNPIQSVTPSTNINEPLVIRKNSGSVVTLTLNSSKQFNALSVKLLAAMQAELDNIAQ
CCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
DKTIQVLVIAANGKAFCAGHNLKEMRANPDVAFQRALFEQCSQIMLTINQMPQVVIAKVQ
CCEEEEEEEEECCCEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
GIATAAGCQLVAACDLAVASDNAKFATSGINVGLFCSTPAVAVSRNLSRKQAFEMLITGE
HHHHHHCCCEEEHHHEEEECCCCEEEECCCCEEEEECCCHHHHHHCCHHHHHHHHHHCCC
FIDAHTALQQGLINRVASAEQLDQMLQSLIDAITNKSSVALKTGKTMFYKQLDMELAEAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHH
EYASEVMSCNMMADDVSEGIDAFIEKRSAIWTGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQFNPIQSVTPSTNINEPLVIRKNSGSVVTLTLNSSKQFNALSVKLLAAMQAELDNIAQ
CCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
DKTIQVLVIAANGKAFCAGHNLKEMRANPDVAFQRALFEQCSQIMLTINQMPQVVIAKVQ
CCEEEEEEEEECCCEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
GIATAAGCQLVAACDLAVASDNAKFATSGINVGLFCSTPAVAVSRNLSRKQAFEMLITGE
HHHHHHCCCEEEHHHEEEECCCCEEEECCCCEEEEECCCHHHHHHCCHHHHHHHHHHCCC
FIDAHTALQQGLINRVASAEQLDQMLQSLIDAITNKSSVALKTGKTMFYKQLDMELAEAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHH
EYASEVMSCNMMADDVSEGIDAFIEKRSAIWTGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA