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The map label for this gene is emrA [H]

Identifier: 93005836

GI number: 93005836

Start: 1206013

End: 1207404

Strand: Reverse

Name: emrA [H]

Synonym: Pcryo_1008

Alternate gene names: 93005836

Gene position: 1207404-1206013 (Counterclockwise)

Preceding gene: 93005837

Following gene: 93005830

Centisome position: 39.46

GC content: 46.12

Gene sequence:

>1392_bases
ATGAACGAAGACAAACCGAACCGCGTTGATGACGCAAGCACACCTAACAAAACAGCTAAAACTGTTGAAAATGCTCAAGC
TAGTGAATCACTATCAAATCAAGCGGTTGTGGATACACCATCACAGTCACACATGGCTTCCAATAATGACGCAAATACCA
ATATAGCAGCCATTCAACCTGAACCGACTATGTCTGCAACTCGGATTCAAACCGACCCTTCTGATGACTTACCTACGAAT
ACCAATGAGCCTATAAACGCCGCTGATACAAATAAAATAGACAGCACTGAGGCAGGCGATGGCAAAGATGATACACCGAT
GCCACCCACTGAATCTATTCCTGCGCCAGCAGGTTGGTCGCCCAAAAAGAAGTCATTTTTTAATTTAGCCTTACTGATTG
CGCTGATTGTTATTGGTGTGGCACTCATTCTCTATGCGTGGAAACTACCACCTTTTACTCCAACCATGCAGCAAACCAAT
AATGCTTTTGTTAAAGGACAAACCACCATTATCAGTCCGCAAGTGTCAGGTTATGTGACAGAAGTAGCGGTAGAAGATTT
TGCGAATGTTGTAGCTGGTGACTTATTGGTAAAAATAGATGACCGTACTTTTCAGCAGCAGCTCGAACAAGCGCGGGCGA
ATATCGATATTGCGTTAACCAATCGCTCTAGTAATGCGCAAGATACTGGCACCAGCCAAGCACAGATTCAGGCACGTGAG
GCAGATGTTTATAGCGCCAAAGTGAGTGTCGACAGTGCCCGAGAGGACGTCAACCGTTATCAGGGCTTGATTGCCATTGG
TGCAGTATCAAGTGCAGATGTTGCCCATGCTGAAGCTCAATTAGCAAAAGCACAAGCTGGTGTCCAACAAGCGGAAGCCA
ATTTACAAGTGGCAGTCGAGGGCAGCAAAAAAACGACGGCTAGCCGCTCTTCATTGGATGCCAATATCAAAAATGCTGAA
GCTGCCGCAAAACAAGCACAGATTAACTTGGACAACACCATAATCCGTGCTCCTGAATCGGGACAACTGAGTCAGTTGAG
CGTTAAAACAGGACAATATGTCACTGCTGGTACGCAGCTTATGTATATCGTACCAAAAGGCGTATGGATTATTGCCAACT
TTAAAGAGACGCAAGTGGCAAATATGACCATTGGTGAAGCGGCCACTATTCATGTGGATGCCTTAGGTGGTACACGGTTT
AGCGGACGGGTCAGCAATATATCACCCGCAACAGGCAGTGAATTCAGTGCTGGCGCTGCCAATCCTGCGACAGGTAACTA
TATTAAAATTGCTCAGCGTATTCCGGTACGCATCGACTTAGACTCTAACCAGCCAGACCTTGCCCGCCTACGTCCTGGTA
TGTCTGTTTCGGTAGATGTTGATACCAAATAG

Upstream 100 bases:

>100_bases
AGATAGTGCAAATGCTATTGTGTATTCTATAGCGCCTATTCTCCCTTATTATTATTGTAACGTTATCTATTATGTTTATA
AAATCCTTTAGGAGTGCGTC

Downstream 100 bases:

>100_bases
CTCCACTATTAAAGAAATAAAAAAAGCCACTTTTTTTGCCATATGAATACGACAGGTAAAGTGGCTTTTTTATAAACATG
AACTTCTTCTAAGCCCATAT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNEDKPNRVDDASTPNKTAKTVENAQASESLSNQAVVDTPSQSHMASNNDANTNIAAIQPEPTMSATRIQTDPSDDLPTN
TNEPINAADTNKIDSTEAGDGKDDTPMPPTESIPAPAGWSPKKKSFFNLALLIALIVIGVALILYAWKLPPFTPTMQQTN
NAFVKGQTTIISPQVSGYVTEVAVEDFANVVAGDLLVKIDDRTFQQQLEQARANIDIALTNRSSNAQDTGTSQAQIQARE
ADVYSAKVSVDSAREDVNRYQGLIAIGAVSSADVAHAEAQLAKAQAGVQQAEANLQVAVEGSKKTTASRSSLDANIKNAE
AAAKQAQINLDNTIIRAPESGQLSQLSVKTGQYVTAGTQLMYIVPKGVWIIANFKETQVANMTIGEAATIHVDALGGTRF
SGRVSNISPATGSEFSAGAANPATGNYIKIAQRIPVRIDLDSNQPDLARLRPGMSVSVDVDTK

Sequences:

>Translated_463_residues
MNEDKPNRVDDASTPNKTAKTVENAQASESLSNQAVVDTPSQSHMASNNDANTNIAAIQPEPTMSATRIQTDPSDDLPTN
TNEPINAADTNKIDSTEAGDGKDDTPMPPTESIPAPAGWSPKKKSFFNLALLIALIVIGVALILYAWKLPPFTPTMQQTN
NAFVKGQTTIISPQVSGYVTEVAVEDFANVVAGDLLVKIDDRTFQQQLEQARANIDIALTNRSSNAQDTGTSQAQIQARE
ADVYSAKVSVDSAREDVNRYQGLIAIGAVSSADVAHAEAQLAKAQAGVQQAEANLQVAVEGSKKTTASRSSLDANIKNAE
AAAKQAQINLDNTIIRAPESGQLSQLSVKTGQYVTAGTQLMYIVPKGVWIIANFKETQVANMTIGEAATIHVDALGGTRF
SGRVSNISPATGSEFSAGAANPATGNYIKIAQRIPVRIDLDSNQPDLARLRPGMSVSVDVDTK
>Mature_463_residues
MNEDKPNRVDDASTPNKTAKTVENAQASESLSNQAVVDTPSQSHMASNNDANTNIAAIQPEPTMSATRIQTDPSDDLPTN
TNEPINAADTNKIDSTEAGDGKDDTPMPPTESIPAPAGWSPKKKSFFNLALLIALIVIGVALILYAWKLPPFTPTMQQTN
NAFVKGQTTIISPQVSGYVTEVAVEDFANVVAGDLLVKIDDRTFQQQLEQARANIDIALTNRSSNAQDTGTSQAQIQARE
ADVYSAKVSVDSAREDVNRYQGLIAIGAVSSADVAHAEAQLAKAQAGVQQAEANLQVAVEGSKKTTASRSSLDANIKNAE
AAAKQAQINLDNTIIRAPESGQLSQLSVKTGQYVTAGTQLMYIVPKGVWIIANFKETQVANMTIGEAATIHVDALGGTRF
SGRVSNISPATGSEFSAGAANPATGNYIKIAQRIPVRIDLDSNQPDLARLRPGMSVSVDVDTK

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=334, Percent_Identity=33.8323353293413, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788711, Length=347, Percent_Identity=32.5648414985591, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790519, Length=308, Percent_Identity=30.5194805194805, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789637, Length=331, Percent_Identity=24.1691842900302, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790024, Length=260, Percent_Identity=24.6153846153846, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790012, Length=272, Percent_Identity=25.3676470588235, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081951, Length=200, Percent_Identity=31, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 49013; Mature: 49013

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEDKPNRVDDASTPNKTAKTVENAQASESLSNQAVVDTPSQSHMASNNDANTNIAAIQP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCEEEEECC
EPTMSATRIQTDPSDDLPTNTNEPINAADTNKIDSTEAGDGKDDTPMPPTESIPAPAGWS
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PKKKSFFNLALLIALIVIGVALILYAWKLPPFTPTMQQTNNAFVKGQTTIISPQVSGYVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCEEEECCCCCCHHH
EVAVEDFANVVAGDLLVKIDDRTFQQQLEQARANIDIALTNRSSNAQDTGTSQAQIQARE
HHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHEEEEEC
ADVYSAKVSVDSAREDVNRYQGLIAIGAVSSADVAHAEAQLAKAQAGVQQAEANLQVAVE
CCEEEEEEEHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
GSKKTTASRSSLDANIKNAEAAAKQAQINLDNTIIRAPESGQLSQLSVKTGQYVTAGTQL
CCCCCCCCHHHCCCCCCHHHHHHHHHEEECCCEEEECCCCCCCEEEEECCCCEEECCCEE
MYIVPKGVWIIANFKETQVANMTIGEAATIHVDALGGTRFSGRVSNISPATGSEFSAGAA
EEEECCCEEEEEECCCCEEEEEEECCEEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCC
NPATGNYIKIAQRIPVRIDLDSNQPDLARLRPGMSVSVDVDTK
CCCCCCEEEEEECCCEEEEECCCCCCHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MNEDKPNRVDDASTPNKTAKTVENAQASESLSNQAVVDTPSQSHMASNNDANTNIAAIQP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCEEEEECC
EPTMSATRIQTDPSDDLPTNTNEPINAADTNKIDSTEAGDGKDDTPMPPTESIPAPAGWS
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PKKKSFFNLALLIALIVIGVALILYAWKLPPFTPTMQQTNNAFVKGQTTIISPQVSGYVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCEEEECCCCCCHHH
EVAVEDFANVVAGDLLVKIDDRTFQQQLEQARANIDIALTNRSSNAQDTGTSQAQIQARE
HHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHEEEEEC
ADVYSAKVSVDSAREDVNRYQGLIAIGAVSSADVAHAEAQLAKAQAGVQQAEANLQVAVE
CCEEEEEEEHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
GSKKTTASRSSLDANIKNAEAAAKQAQINLDNTIIRAPESGQLSQLSVKTGQYVTAGTQL
CCCCCCCCHHHCCCCCCHHHHHHHHHEEECCCEEEECCCCCCCEEEEECCCCEEECCCEE
MYIVPKGVWIIANFKETQVANMTIGEAATIHVDALGGTRFSGRVSNISPATGSEFSAGAA
EEEECCCEEEEEECCCCEEEEEEECCEEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCC
NPATGNYIKIAQRIPVRIDLDSNQPDLARLRPGMSVSVDVDTK
CCCCCCEEEEEECCCEEEEECCCCCCHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]