Click here to switch to the map view.

The map label for this gene is mcrB [H]

Identifier: 93005767

GI number: 93005767

Start: 1116101

End: 1118533

Strand: Direct

Name: mcrB [H]

Synonym: Pcryo_0939

Alternate gene names: 93005767

Gene position: 1116101-1118533 (Clockwise)

Preceding gene: 93005765

Following gene: 93005768

Centisome position: 36.48

GC content: 38.27

Gene sequence:

>2433_bases
ATGATGACTGAAAATTTTGAAGCAATAGATGTAAATTCTTATTGGTTTGTTGGTGCTGTATATAATGGGATAGACCAAAC
GTCTCGATTTGTTGAAACAGGTATCTGGGAGGTTAATCCTGAAGCCAATCAGATTGATTCAGTAAAGTCTATTGAGGCTG
GTGATAGGATCGCTATTAAATCAACCTATACTAGAAAAAACGAACTACCTTTTGATAATCGAGGCCACACAGTTTCTGTG
ATGTTAATTAAGGCTATCGGTACCGTTATTGAGAATGTAGGTGACGGTAACCTTTTAAAAGTAGATTGGGACAACAGCTT
TATTGCTAAAGAATGGTATTTTTTCACTAGTCGAAATACAGTTTGGAGAGTCATTCCTGAGACTGCGTTAAGAGTTAATT
TGATTGATTTTGCATTTGACGGTAAAGCTCAAGACATAGATATGTTCCGTAATAATCCTTATTGGCTTGAAAGATTTGGC
GATTCACCTTCAGAGCATTCCGCTTTTGAGTGGACAACTTTCTATGAAGAAATGGCCTCTAAGCTTCTTGCCTTTCGCTA
TCGTCGTGATGAGCTTGTCACCGGTATTCATAAAATATCTGAAACAAGTAATGTCATGTCTATTCTTAACGACCAATACG
AAGAGGGTGTCGAAGGAGGATTATTAAAAGATATATGTCCTTTTACAGTCATGGCTCTTTTTAATCGTGGAATCACTGAC
GATAATCGCAAAATTATAGCTAAAGCATTGGCTGAACTACTTGATTTAACAGAGCCTGTTCCTGAATCTTTTGATGGCAT
TCCTATAGTTAACAACCAAAGGACATGGTTTTTTAGTTATAGCTATAAGCGTCATCCAGATGATATAGATATTCTATGGG
AGGTGTTTGCTCAAGCCATCGCATTTGCCGACTTAGACGGTGAAATTGAAGATGATAGATTCCAGTTTATGGCTGCATAT
GATAAAGCAACGCACTGCCATAGAGTGGGTTGGAATCTCACTATGGGTCTATATTGGATTAGGCCTTGGAAATTCTTAAC
GCTAGATAGACAATCTCAAGAATACATCAATAAAAAGCTTGATATCGAAATCAATAAAAACGGTCCAAAAGCACGCTGTG
ATGCTAATGATTATTTAAAGTTGTTAGAGAGTTTTGAAACCAGATTTTTAGAAGAAACTTATCCTGTACATTCTTTTCCT
GAACTCTCGCTTGTAGCAGATAAGTATCAGCCTAATGATTTGGAGAAGATATCTAAAAATAACTGGAAATATATTATTCC
ATCTCAAGTGAAACAGCTCTGTCATGACAAGCAGTTTAATGAATTTACGGTAGATGAATTTTTAAAAAGGTTTACTAAGG
ATCTTGAACAAAGATATCCTAAGAACAGTGACGTGAAAAGAGATATTAAATATGTTTTAAGAGAAATGGCTAAAAATGGA
AGTTTGGAGTCACTTAGTAAAGGAGTGTATCGCTTGCTGAATGTAAATATGACCAGTGATCTTGATGTTTCAACCTATTC
TGAGCTTGATATTCAAACAGTCCCTGATACTTACTCTGCTACTAATATTACATCTGATGGGTGTTTTTTGCCTAAAGCTA
AGATTGAGATGATACTCAAACGTTTTCGAGCTAAGAAAAATCTAATACTACAAGGGCCTCCCGGTACTGGTAAAACTTGG
TTGGCTAAAAGATTAGCCTTTGCTTTAATGGGTCAGCGTGACGATAGCAAAATACGAGCCGTGCAATTTCATCCTAATTT
GTCTTATGAAGATTTTATTAGAGGTTGGCGTCCATCAGGTGAGGGCAAGCTGACATTGGTAGACGGTCCTTTTATGGAAA
TGATCAAAGCCGCTATTGATGATCCTGAATCAAAGTACGTTATTGTCATCGAAGAGATTAACCGAGGTAACCCTGCTCAA
GTGTTCGGTGAGATGCTCACACTACTTGAAACCGATAAAAGAACCCCAGACGAAGCGTTAGAGCTGTCTTATAGAAAGTT
CGATGGTGAACGTGTTTATATTCCCAGTAATTTATATGTCATAGGCACAATGAATATAGCTGACCGTTCGCTGGCCTTGG
TTGATCTTGCGCTACGTCGTCGGTTTGCTTTTATTGATCTTGAGCCAACATTAGGCAAGCCATGGCATGATTGGGTTCAA
TCCAAAAATGGCATTGATTCAACGATTTTAAATGCAATTGACAGTCGCATCAGGGCTCTAAATGAGGATATTGCTGCAGA
TACCAGTCTTGGTGCTCAGTTTCAAATAGGCCATAGCTATGTCACGCCAGCCGTTGGGATGAATATAACGGATGCTAAGG
AATGGTTTAGTGAGGTGGTACAGACGGAGATTGGTCCTTTACTGGATGAGTACTGGTTTGATGATCTTGAGAAGTCGCAA
AAAGCGCAGAAACGCTTGATTGAAGGTCTGTAA

Upstream 100 bases:

>100_bases
ACTAATTTTTATTTTAAATAAATGGTATCTTAGACACTTACGCGTAATCAGATATTAAATAGAATACTTTAATCAGAAAG
TATAACATTAACAGGATTTT

Downstream 100 bases:

>100_bases
GTCTATGTCTATTGAAATAGATTCGCTGTCTGAATATTATGATGAGATCAAGTCTATAGGCAAAATACCTATACGCAACC
TTTGGCTGCTTATGCTTTAT

Product: ATPase

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 810; Mature: 810

Protein sequence:

>810_residues
MMTENFEAIDVNSYWFVGAVYNGIDQTSRFVETGIWEVNPEANQIDSVKSIEAGDRIAIKSTYTRKNELPFDNRGHTVSV
MLIKAIGTVIENVGDGNLLKVDWDNSFIAKEWYFFTSRNTVWRVIPETALRVNLIDFAFDGKAQDIDMFRNNPYWLERFG
DSPSEHSAFEWTTFYEEMASKLLAFRYRRDELVTGIHKISETSNVMSILNDQYEEGVEGGLLKDICPFTVMALFNRGITD
DNRKIIAKALAELLDLTEPVPESFDGIPIVNNQRTWFFSYSYKRHPDDIDILWEVFAQAIAFADLDGEIEDDRFQFMAAY
DKATHCHRVGWNLTMGLYWIRPWKFLTLDRQSQEYINKKLDIEINKNGPKARCDANDYLKLLESFETRFLEETYPVHSFP
ELSLVADKYQPNDLEKISKNNWKYIIPSQVKQLCHDKQFNEFTVDEFLKRFTKDLEQRYPKNSDVKRDIKYVLREMAKNG
SLESLSKGVYRLLNVNMTSDLDVSTYSELDIQTVPDTYSATNITSDGCFLPKAKIEMILKRFRAKKNLILQGPPGTGKTW
LAKRLAFALMGQRDDSKIRAVQFHPNLSYEDFIRGWRPSGEGKLTLVDGPFMEMIKAAIDDPESKYVIVIEEINRGNPAQ
VFGEMLTLLETDKRTPDEALELSYRKFDGERVYIPSNLYVIGTMNIADRSLALVDLALRRRFAFIDLEPTLGKPWHDWVQ
SKNGIDSTILNAIDSRIRALNEDIAADTSLGAQFQIGHSYVTPAVGMNITDAKEWFSEVVQTEIGPLLDEYWFDDLEKSQ
KAQKRLIEGL

Sequences:

>Translated_810_residues
MMTENFEAIDVNSYWFVGAVYNGIDQTSRFVETGIWEVNPEANQIDSVKSIEAGDRIAIKSTYTRKNELPFDNRGHTVSV
MLIKAIGTVIENVGDGNLLKVDWDNSFIAKEWYFFTSRNTVWRVIPETALRVNLIDFAFDGKAQDIDMFRNNPYWLERFG
DSPSEHSAFEWTTFYEEMASKLLAFRYRRDELVTGIHKISETSNVMSILNDQYEEGVEGGLLKDICPFTVMALFNRGITD
DNRKIIAKALAELLDLTEPVPESFDGIPIVNNQRTWFFSYSYKRHPDDIDILWEVFAQAIAFADLDGEIEDDRFQFMAAY
DKATHCHRVGWNLTMGLYWIRPWKFLTLDRQSQEYINKKLDIEINKNGPKARCDANDYLKLLESFETRFLEETYPVHSFP
ELSLVADKYQPNDLEKISKNNWKYIIPSQVKQLCHDKQFNEFTVDEFLKRFTKDLEQRYPKNSDVKRDIKYVLREMAKNG
SLESLSKGVYRLLNVNMTSDLDVSTYSELDIQTVPDTYSATNITSDGCFLPKAKIEMILKRFRAKKNLILQGPPGTGKTW
LAKRLAFALMGQRDDSKIRAVQFHPNLSYEDFIRGWRPSGEGKLTLVDGPFMEMIKAAIDDPESKYVIVIEEINRGNPAQ
VFGEMLTLLETDKRTPDEALELSYRKFDGERVYIPSNLYVIGTMNIADRSLALVDLALRRRFAFIDLEPTLGKPWHDWVQ
SKNGIDSTILNAIDSRIRALNEDIAADTSLGAQFQIGHSYVTPAVGMNITDAKEWFSEVVQTEIGPLLDEYWFDDLEKSQ
KAQKRLIEGL
>Mature_810_residues
MMTENFEAIDVNSYWFVGAVYNGIDQTSRFVETGIWEVNPEANQIDSVKSIEAGDRIAIKSTYTRKNELPFDNRGHTVSV
MLIKAIGTVIENVGDGNLLKVDWDNSFIAKEWYFFTSRNTVWRVIPETALRVNLIDFAFDGKAQDIDMFRNNPYWLERFG
DSPSEHSAFEWTTFYEEMASKLLAFRYRRDELVTGIHKISETSNVMSILNDQYEEGVEGGLLKDICPFTVMALFNRGITD
DNRKIIAKALAELLDLTEPVPESFDGIPIVNNQRTWFFSYSYKRHPDDIDILWEVFAQAIAFADLDGEIEDDRFQFMAAY
DKATHCHRVGWNLTMGLYWIRPWKFLTLDRQSQEYINKKLDIEINKNGPKARCDANDYLKLLESFETRFLEETYPVHSFP
ELSLVADKYQPNDLEKISKNNWKYIIPSQVKQLCHDKQFNEFTVDEFLKRFTKDLEQRYPKNSDVKRDIKYVLREMAKNG
SLESLSKGVYRLLNVNMTSDLDVSTYSELDIQTVPDTYSATNITSDGCFLPKAKIEMILKRFRAKKNLILQGPPGTGKTW
LAKRLAFALMGQRDDSKIRAVQFHPNLSYEDFIRGWRPSGEGKLTLVDGPFMEMIKAAIDDPESKYVIVIEEINRGNPAQ
VFGEMLTLLETDKRTPDEALELSYRKFDGERVYIPSNLYVIGTMNIADRSLALVDLALRRRFAFIDLEPTLGKPWHDWVQ
SKNGIDSTILNAIDSRIRALNEDIAADTSLGAQFQIGHSYVTPAVGMNITDAKEWFSEVVQTEIGPLLDEYWFDDLEKSQ
KAQKRLIEGL

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=281, Percent_Identity=45.5516014234875, Blast_Score=260, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 93364; Mature: 93364

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTENFEAIDVNSYWFVGAVYNGIDQTSRFVETGIWEVNPEANQIDSVKSIEAGDRIAIK
CCCCCCCEEECCCEEEEHHHHHHHHHHHHHHHCCCEEECCCCHHHCCHHHCCCCCEEEEE
STYTRKNELPFDNRGHTVSVMLIKAIGTVIENVGDGNLLKVDWDNSFIAKEWYFFTSRNT
ECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCC
VWRVIPETALRVNLIDFAFDGKAQDIDMFRNNPYWLERFGDSPSEHSAFEWTTFYEEMAS
EEEEECCCCEEEEEEEEEECCCCCCHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHH
KLLAFRYRRDELVTGIHKISETSNVMSILNDQYEEGVEGGLLKDICPFTVMALFNRGITD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
DNRKIIAKALAELLDLTEPVPESFDGIPIVNNQRTWFFSYSYKRHPDDIDILWEVFAQAI
CHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHH
AFADLDGEIEDDRFQFMAAYDKATHCHRVGWNLTMGLYWIRPWKFLTLDRQSQEYINKKL
HHHCCCCCCCCCHHHHHHHHHHCCHHHHCCCEEEEEEEEECCEEEEEECCHHHHHCCCEE
DIEINKNGPKARCDANDYLKLLESFETRFLEETYPVHSFPELSLVADKYQPNDLEKISKN
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCCCHHHHHHCC
NWKYIIPSQVKQLCHDKQFNEFTVDEFLKRFTKDLEQRYPKNSDVKRDIKYVLREMAKNG
CCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
SLESLSKGVYRLLNVNMTSDLDVSTYSELDIQTVPDTYSATNITSDGCFLPKAKIEMILK
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
RFRAKKNLILQGPPGTGKTWLAKRLAFALMGQRDDSKIRAVQFHPNLSYEDFIRGWRPSG
HHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCCCC
EGKLTLVDGPFMEMIKAAIDDPESKYVIVIEEINRGNPAQVFGEMLTLLETDKRTPDEAL
CCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHH
ELSYRKFDGERVYIPSNLYVIGTMNIADRSLALVDLALRRRFAFIDLEPTLGKPWHDWVQ
HHHHHEECCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHH
SKNGIDSTILNAIDSRIRALNEDIAADTSLGAQFQIGHSYVTPAVGMNITDAKEWFSEVV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECHHCCCCCCHHHHHHHHHH
QTEIGPLLDEYWFDDLEKSQKAQKRLIEGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMTENFEAIDVNSYWFVGAVYNGIDQTSRFVETGIWEVNPEANQIDSVKSIEAGDRIAIK
CCCCCCCEEECCCEEEEHHHHHHHHHHHHHHHCCCEEECCCCHHHCCHHHCCCCCEEEEE
STYTRKNELPFDNRGHTVSVMLIKAIGTVIENVGDGNLLKVDWDNSFIAKEWYFFTSRNT
ECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCC
VWRVIPETALRVNLIDFAFDGKAQDIDMFRNNPYWLERFGDSPSEHSAFEWTTFYEEMAS
EEEEECCCCEEEEEEEEEECCCCCCHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHH
KLLAFRYRRDELVTGIHKISETSNVMSILNDQYEEGVEGGLLKDICPFTVMALFNRGITD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
DNRKIIAKALAELLDLTEPVPESFDGIPIVNNQRTWFFSYSYKRHPDDIDILWEVFAQAI
CHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHH
AFADLDGEIEDDRFQFMAAYDKATHCHRVGWNLTMGLYWIRPWKFLTLDRQSQEYINKKL
HHHCCCCCCCCCHHHHHHHHHHCCHHHHCCCEEEEEEEEECCEEEEEECCHHHHHCCCEE
DIEINKNGPKARCDANDYLKLLESFETRFLEETYPVHSFPELSLVADKYQPNDLEKISKN
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCCCHHHHHHCC
NWKYIIPSQVKQLCHDKQFNEFTVDEFLKRFTKDLEQRYPKNSDVKRDIKYVLREMAKNG
CCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
SLESLSKGVYRLLNVNMTSDLDVSTYSELDIQTVPDTYSATNITSDGCFLPKAKIEMILK
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
RFRAKKNLILQGPPGTGKTWLAKRLAFALMGQRDDSKIRAVQFHPNLSYEDFIRGWRPSG
HHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCCCC
EGKLTLVDGPFMEMIKAAIDDPESKYVIVIEEINRGNPAQVFGEMLTLLETDKRTPDEAL
CCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHH
ELSYRKFDGERVYIPSNLYVIGTMNIADRSLALVDLALRRRFAFIDLEPTLGKPWHDWVQ
HHHHHEECCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHH
SKNGIDSTILNAIDSRIRALNEDIAADTSLGAQFQIGHSYVTPAVGMNITDAKEWFSEVV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECHHCCCCCCHHHHHHHHHH
QTEIGPLLDEYWFDDLEKSQKAQKRLIEGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]