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The map label for this gene is mcl2 [H]

Identifier: 93005693

GI number: 93005693

Start: 1023137

End: 1023967

Strand: Direct

Name: mcl2 [H]

Synonym: Pcryo_0865

Alternate gene names: 93005693

Gene position: 1023137-1023967 (Clockwise)

Preceding gene: 93005692

Following gene: 93005695

Centisome position: 33.44

GC content: 42.36

Gene sequence:

>831_bases
ATGCAAGCATCTATTGTTAAAAAATTTTCCAATATCAAAACATGGTTATTTGTTCCAGCCACTCGCATAGATAGAGTGGG
AAAAGCCTTTGCCAGTGGGACAGATGCAGTGATTGTGGATTTAGAAGATGCGGTTGCTACAACGGACAAAGTGCAAGCTC
GCAAAGATTTACAAGTTTATTATAATAGTCAGGCTTATCAGCCGATTTGGGTACGCATCAATAAAGCAGGTAGTGAGGAG
TTTTTCAAAGACGTAGTGCTCTGTCAGAAAATGCCTAATTTGGCTGGCGTGTTACTAGCCAAAGCTGAGCAAGCATCAGA
TATTGAAAACGCTCATAACATGACTGGACTGCCCGTCATTGCGTTAATAGAGACAGCGCTTGGTTTATATCAAATCGATA
GTATGGCAAAATCTGCTGGACTGCTTGCATTTAGCTATGGGTTTTTAGATTTATGCAATGATTTACAGGTGCACGTAGGT
ACAGCGGCAGCAGATGTTATTGCTAATCAAATTCGTTATCAGCTTTTACTCACATCAAAAGTACATCAATTATTAGCGCC
TATTGATACAGTTTATACTGATTTTAATGATGGTAAGGGGCTTGGGCAGCGAGTGCAATTATGGTCACAAATGGGCATGT
CCGGAATGCTTTGTATCCATCCAAAGCAAGTAGCAACTGTTAAGCAATCTCTGCAACCGACTGACGCCGATATTGAGTTT
GCAAGACGTGTGATAGAAGAATACGAGCGGAGCGGGCAAGCGGTATTTAAAATAGCAGGTCAAATGGTCGACGCGCCCGT
AATTGAGCGCTGCCGTCAGTTGCTGAGTTGA

Upstream 100 bases:

>100_bases
ATACGGTTTTTTATTTGGTTATAGTAAGTACTAATATTTTAAGTAACAGTGTTTGAAATAATAATGTTTGAAGTAATAGT
ATTTAAGGAAGGAATAAAGC

Downstream 100 bases:

>100_bases
TAAAAGATTTTTAATAAAATGTGCCCTAATAGACTAAAACAGCAAAAAAGGCGCGGTACGACTGGCTTTTGACTTCGCAA
CCTCTGCTGGTGTGCCTTCT

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQASIVKKFSNIKTWLFVPATRIDRVGKAFASGTDAVIVDLEDAVATTDKVQARKDLQVYYNSQAYQPIWVRINKAGSEE
FFKDVVLCQKMPNLAGVLLAKAEQASDIENAHNMTGLPVIALIETALGLYQIDSMAKSAGLLAFSYGFLDLCNDLQVHVG
TAAADVIANQIRYQLLLTSKVHQLLAPIDTVYTDFNDGKGLGQRVQLWSQMGMSGMLCIHPKQVATVKQSLQPTDADIEF
ARRVIEEYERSGQAVFKIAGQMVDAPVIERCRQLLS

Sequences:

>Translated_276_residues
MQASIVKKFSNIKTWLFVPATRIDRVGKAFASGTDAVIVDLEDAVATTDKVQARKDLQVYYNSQAYQPIWVRINKAGSEE
FFKDVVLCQKMPNLAGVLLAKAEQASDIENAHNMTGLPVIALIETALGLYQIDSMAKSAGLLAFSYGFLDLCNDLQVHVG
TAAADVIANQIRYQLLLTSKVHQLLAPIDTVYTDFNDGKGLGQRVQLWSQMGMSGMLCIHPKQVATVKQSLQPTDADIEF
ARRVIEEYERSGQAVFKIAGQMVDAPVIERCRQLLS
>Mature_276_residues
MQASIVKKFSNIKTWLFVPATRIDRVGKAFASGTDAVIVDLEDAVATTDKVQARKDLQVYYNSQAYQPIWVRINKAGSEE
FFKDVVLCQKMPNLAGVLLAKAEQASDIENAHNMTGLPVIALIETALGLYQIDSMAKSAGLLAFSYGFLDLCNDLQVHVG
TAAADVIANQIRYQLLLTSKVHQLLAPIDTVYTDFNDGKGLGQRVQLWSQMGMSGMLCIHPKQVATVKQSLQPTDADIEF
ARRVIEEYERSGQAVFKIAGQMVDAPVIERCRQLLS

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=24.7457627118644, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI87081764, Length=295, Percent_Identity=28.4745762711864, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557458, Length=292, Percent_Identity=26.3698630136986, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30427; Mature: 30427

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASIVKKFSNIKTWLFVPATRIDRVGKAFASGTDAVIVDLEDAVATTDKVQARKDLQVY
CCHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHEE
YNSQAYQPIWVRINKAGSEEFFKDVVLCQKMPNLAGVLLAKAEQASDIENAHNMTGLPVI
ECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ALIETALGLYQIDSMAKSAGLLAFSYGFLDLCNDLQVHVGTAAADVIANQIRYQLLLTSK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHEEHHHHHHHHHHHHHHHHHHHHHH
VHQLLAPIDTVYTDFNDGKGLGQRVQLWSQMGMSGMLCIHPKQVATVKQSLQPTDADIEF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCCHHH
ARRVIEEYERSGQAVFKIAGQMVDAPVIERCRQLLS
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MQASIVKKFSNIKTWLFVPATRIDRVGKAFASGTDAVIVDLEDAVATTDKVQARKDLQVY
CCHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHEE
YNSQAYQPIWVRINKAGSEEFFKDVVLCQKMPNLAGVLLAKAEQASDIENAHNMTGLPVI
ECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ALIETALGLYQIDSMAKSAGLLAFSYGFLDLCNDLQVHVGTAAADVIANQIRYQLLLTSK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHEEHHHHHHHHHHHHHHHHHHHHHH
VHQLLAPIDTVYTDFNDGKGLGQRVQLWSQMGMSGMLCIHPKQVATVKQSLQPTDADIEF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCCHHH
ARRVIEEYERSGQAVFKIAGQMVDAPVIERCRQLLS
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA