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The map label for this gene is opuD [H]

Identifier: 93005673

GI number: 93005673

Start: 1001091

End: 1002746

Strand: Direct

Name: opuD [H]

Synonym: Pcryo_0845

Alternate gene names: 93005673

Gene position: 1001091-1002746 (Clockwise)

Preceding gene: 93005672

Following gene: 93005675

Centisome position: 32.72

GC content: 44.69

Gene sequence:

>1656_bases
ATGGATCATGTAAAAGTTGGCCGCTTGGGCCCTTTTCCACGGGTAAGTAAACCTGTGTTCTTAACTTCAGCACTATTAAT
TCTGGTGTTTATCATCTTTGGTGCTTTTTTTAATGAGCAAGCAGAACTGGTATTTGGTCACGCAAAAGCATTCGTGTCTT
TGCGCTTTGGCTGGTTCTTTATTGTGGTGGTTAACTTAACGCTAGTGATGAGTATTTATATGATATTCAGTCGCTATGGC
GATATCAGGCTTGGTCATCAGAACGAGAAGCCCGAGTATAATATAGTCTCCTGGATTGGCATGCTGTTTTCAGCTGGCAT
CGGTATTGGACTGCTCTATTGGGGCACCGCTGAACCCTTGTACCATTTTATGGCGCCGCCATTGGGAGAGCCCGAAACGA
TCGCCGCCGCCAAGCAAGCGATGAATATCTCGTTCCTGCATTATGGCTTACATGTGTGGGCGCTCTACGGCATGGTTGCT
TTGTCATTGGCTTATTTTCACTACCGAGTGGGTTTACCACTGGCCATCCGCTCGACACTTTATCCTATATTGGGTAAGAA
AATCTATGGTGGTTGGGGTCATACGGTCGATACGCTGGCGGTATTTGGGACGATGTTTGGGGTAGTGACCACGTTAGGTC
TTGGGGTATTGCAGATTAACTCAGGTCTTGAGACGTTATTTGGTATTCCCAATAATATTACAGTGCAAATTATATTGATT
GCCTTAATCACTATGCTGGCTGGTCTATCTCTGTTTATGGGATTAGATAAAGGGATTAAGCGCTTAAGTGATACCAATAT
ATTATTTACTGTGATATTGCTAAGCTTTGTGATTATTTTAGGACCGACTCAGTTTATTATAAATAGCTTTGTAGAGAACA
TCGGCAACTATTTACATCAGATTGTGCCACTAGGTCTTTGGACAGAGTCTTATAACGGTGAAGAAAACTGGCAAGCATCA
TGGACGATTTTCTATTGGGCATGGTGGATAAGCTGGTCGCCTTTCGTTGGGGTGTTCGTGGCTCGTATCTCTCGTGGTCG
TACGATTCGTGAATTCATATTGGGAGTGTTGTTGATTCCTATTACCATATTGTTCTTATGGTTTACCGCTTTTGGTGGTT
CAGCCGTTAATATGGAGCTAATGGCGGCGGCGGACCCCAATATTATCAGTCCTGGCTTGATCGAGGCGGTCCAAGCAGAT
ACTGGCAGTGCCATCTTTAAGTTGATGGAGTCTTATCCGCTAACATCGGCTGTGAACTTGCTCATCGTGGTAATGATTGT
TCTTTGGTTTGTCACGTCATCGGATTCAGCCAGTTTCGTCATTGATATGTTGACCTCAGGTGGCGATGCTAATCCACCGA
AAATTCAGCGCTTATTTTGGGCGGGTACCGAAGGTGTCATCGCAGCGGTACTATTGGCAGCAGGTGGCTTGAGTGCACTA
CAAGCGGCTTCCATTGTGTCAGGTTTTCCTTTTGCGATAGTGATAGTCGTAATGATGTATTCGCTACTACGTGGCTTAAG
TCGTGACCGTTTGATATTGTATCGCAATCAGCAATGGTTTATCACCGAGGAATCTGCTGAGCACAATTCAGCCAATGAAT
TTCGTGATGAAGACTTGCTCACAGGTCCGCCTGATATTGAAAAGCCTGAAGGTTAA

Upstream 100 bases:

>100_bases
TATTTGTTGACCAATAGCAAGGTTATAAAGTGTCTGATTCGATTTGATAGTTGTGTAATATCTTGTTAAGGTGAGCCCCT
TCAAAAACTGAGACGACTAA

Downstream 100 bases:

>100_bases
GAGTTTTTTAGACAATGCTTAAATAGTACTTAAGCTAAAAAATAATTCAAAAAGCCCCAGCTTTATCATAAGTTGGGGCT
TTTTAATTTCTACTGATATA

Product: choline/carnitine/betaine transport protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MDHVKVGRLGPFPRVSKPVFLTSALLILVFIIFGAFFNEQAELVFGHAKAFVSLRFGWFFIVVVNLTLVMSIYMIFSRYG
DIRLGHQNEKPEYNIVSWIGMLFSAGIGIGLLYWGTAEPLYHFMAPPLGEPETIAAAKQAMNISFLHYGLHVWALYGMVA
LSLAYFHYRVGLPLAIRSTLYPILGKKIYGGWGHTVDTLAVFGTMFGVVTTLGLGVLQINSGLETLFGIPNNITVQIILI
ALITMLAGLSLFMGLDKGIKRLSDTNILFTVILLSFVIILGPTQFIINSFVENIGNYLHQIVPLGLWTESYNGEENWQAS
WTIFYWAWWISWSPFVGVFVARISRGRTIREFILGVLLIPITILFLWFTAFGGSAVNMELMAAADPNIISPGLIEAVQAD
TGSAIFKLMESYPLTSAVNLLIVVMIVLWFVTSSDSASFVIDMLTSGGDANPPKIQRLFWAGTEGVIAAVLLAAGGLSAL
QAASIVSGFPFAIVIVVMMYSLLRGLSRDRLILYRNQQWFITEESAEHNSANEFRDEDLLTGPPDIEKPEG

Sequences:

>Translated_551_residues
MDHVKVGRLGPFPRVSKPVFLTSALLILVFIIFGAFFNEQAELVFGHAKAFVSLRFGWFFIVVVNLTLVMSIYMIFSRYG
DIRLGHQNEKPEYNIVSWIGMLFSAGIGIGLLYWGTAEPLYHFMAPPLGEPETIAAAKQAMNISFLHYGLHVWALYGMVA
LSLAYFHYRVGLPLAIRSTLYPILGKKIYGGWGHTVDTLAVFGTMFGVVTTLGLGVLQINSGLETLFGIPNNITVQIILI
ALITMLAGLSLFMGLDKGIKRLSDTNILFTVILLSFVIILGPTQFIINSFVENIGNYLHQIVPLGLWTESYNGEENWQAS
WTIFYWAWWISWSPFVGVFVARISRGRTIREFILGVLLIPITILFLWFTAFGGSAVNMELMAAADPNIISPGLIEAVQAD
TGSAIFKLMESYPLTSAVNLLIVVMIVLWFVTSSDSASFVIDMLTSGGDANPPKIQRLFWAGTEGVIAAVLLAAGGLSAL
QAASIVSGFPFAIVIVVMMYSLLRGLSRDRLILYRNQQWFITEESAEHNSANEFRDEDLLTGPPDIEKPEG
>Mature_551_residues
MDHVKVGRLGPFPRVSKPVFLTSALLILVFIIFGAFFNEQAELVFGHAKAFVSLRFGWFFIVVVNLTLVMSIYMIFSRYG
DIRLGHQNEKPEYNIVSWIGMLFSAGIGIGLLYWGTAEPLYHFMAPPLGEPETIAAAKQAMNISFLHYGLHVWALYGMVA
LSLAYFHYRVGLPLAIRSTLYPILGKKIYGGWGHTVDTLAVFGTMFGVVTTLGLGVLQINSGLETLFGIPNNITVQIILI
ALITMLAGLSLFMGLDKGIKRLSDTNILFTVILLSFVIILGPTQFIINSFVENIGNYLHQIVPLGLWTESYNGEENWQAS
WTIFYWAWWISWSPFVGVFVARISRGRTIREFILGVLLIPITILFLWFTAFGGSAVNMELMAAADPNIISPGLIEAVQAD
TGSAIFKLMESYPLTSAVNLLIVVMIVLWFVTSSDSASFVIDMLTSGGDANPPKIQRLFWAGTEGVIAAVLLAAGGLSAL
QAASIVSGFPFAIVIVVMMYSLLRGLSRDRLILYRNQQWFITEESAEHNSANEFRDEDLLTGPPDIEKPEG

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=521, Percent_Identity=39.1554702495201, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1786224, Length=479, Percent_Identity=30.062630480167, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788102, Length=454, Percent_Identity=30.6167400881057, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 60893; Mature: 60893

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]