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The map label for this gene is greA [H]

Identifier: 93005658

GI number: 93005658

Start: 979621

End: 980097

Strand: Reverse

Name: greA [H]

Synonym: Pcryo_0828

Alternate gene names: 93005658

Gene position: 980097-979621 (Counterclockwise)

Preceding gene: 93005659

Following gene: 93005657

Centisome position: 32.03

GC content: 45.28

Gene sequence:

>477_bases
ATGCAACGTTATCCCATGACTCCGCAAGGACATGCGGCTTTAGAAGCCGAATTAAAACAGTTAAAAAGTATTGACCGTCC
TCGCATCACCGCCTCTATTGCTGAAGCACGCGAACATGGTGATTTGAAAGAAAATGCTGAATATCATGCAGCGCGTGAAC
AGCAGGGATTTTGTGAAGCTCGTATCCGTGATATCGAAGCCAAACTTGGCGGTGCGCAAGTGATTGATCCTACGACGTTA
CCAAAAGATGGCCGTGTGATATTCGGCGTCAGCGTCGTTATTGAAAACATGGACACTGAAGAGGAAAAGCAGTACAAAAT
CGTTGGTGACGACGAAGCGGATTTCAAAGCCGGTAAAATCTCTGTTAACTCACCGATTGCGCGTGGTTTGATTGGTAAAT
CTGAGGGCGATGAAGCGCGTATTGAAACCCCAAAAGGTGTGGTTGAATACGAAATTATGAAAGTCATCTACGATTAA

Upstream 100 bases:

>100_bases
TTCTTTTTTTATTTTATACCCATTGCAACAACTCTGACACCATCTCACTATTCACTAAGAGTAGGAGATGGTGCCCGTGT
TATTGGTTTAAGGAAAAAAC

Downstream 100 bases:

>100_bases
TATAGTCGTCTTTTGTAGTAAATCAAATAGTAACTTTCTTAAAAAATTAGATAAAGTAAACCACACAAAAAGTCATCATT
TATAGGATAGCAGTTACACA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MQRYPMTPQGHAALEAELKQLKSIDRPRITASIAEAREHGDLKENAEYHAAREQQGFCEARIRDIEAKLGGAQVIDPTTL
PKDGRVIFGVSVVIENMDTEEEKQYKIVGDDEADFKAGKISVNSPIARGLIGKSEGDEARIETPKGVVEYEIMKVIYD

Sequences:

>Translated_158_residues
MQRYPMTPQGHAALEAELKQLKSIDRPRITASIAEAREHGDLKENAEYHAAREQQGFCEARIRDIEAKLGGAQVIDPTTL
PKDGRVIFGVSVVIENMDTEEEKQYKIVGDDEADFKAGKISVNSPIARGLIGKSEGDEARIETPKGVVEYEIMKVIYD
>Mature_158_residues
MQRYPMTPQGHAALEAELKQLKSIDRPRITASIAEAREHGDLKENAEYHAAREQQGFCEARIRDIEAKLGGAQVIDPTTL
PKDGRVIFGVSVVIENMDTEEEKQYKIVGDDEADFKAGKISVNSPIARGLIGKSEGDEARIETPKGVVEYEIMKVIYD

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=67.515923566879, Blast_Score=214, Evalue=2e-57,
Organism=Escherichia coli, GI87082261, Length=152, Percent_Identity=32.2368421052632, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17542; Mature: 17542

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYPMTPQGHAALEAELKQLKSIDRPRITASIAEAREHGDLKENAEYHAAREQQGFCEA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHH
RIRDIEAKLGGAQVIDPTTLPKDGRVIFGVSVVIENMDTEEEKQYKIVGDDEADFKAGKI
HHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCEE
SVNSPIARGLIGKSEGDEARIETPKGVVEYEIMKVIYD
ECCCCHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQRYPMTPQGHAALEAELKQLKSIDRPRITASIAEAREHGDLKENAEYHAAREQQGFCEA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHH
RIRDIEAKLGGAQVIDPTTLPKDGRVIFGVSVVIENMDTEEEKQYKIVGDDEADFKAGKI
HHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCEE
SVNSPIARGLIGKSEGDEARIETPKGVVEYEIMKVIYD
ECCCCHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA