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The map label for this gene is metZ [H]

Identifier: 93005628

GI number: 93005628

Start: 940904

End: 942172

Strand: Direct

Name: metZ [H]

Synonym: Pcryo_0798

Alternate gene names: 93005628

Gene position: 940904-942172 (Clockwise)

Preceding gene: 93005624

Following gene: 93005629

Centisome position: 30.75

GC content: 45.15

Gene sequence:

>1269_bases
ATGAGTCAACCGCAAAATAAAGACAATATAAACGGCTTGGATGCAAACTGGTGCAATGATGCACTGGATTTAGAACTCGA
TTATGGTATGCAAACCATTGCAGTACGTGCAGGTCAGCATCGTACCGATGAGGGCGAACATTCAGAAGCTATTTTTACGA
CCAGCTCTTATGTATATAGCTCAGCAGCTGATGCTGCCGGTCATTTTGATGGCACTAAAACGGGCAATGTCTACTCACGT
CATACCAATCCAAGCGTACGTACCTTTGAGCGCCGTTTAGCAGCACTTGAAAATGGTGAGCGTGCAGTCGCAACAGCCTC
TGGCATGGGTGCGATTTTGACCATGTGCTTGGCGTACTTAAAAGCCGGTAACCATTTACTTGCTGCTAATCAGCTATTTG
GCTCCTCTATTGGTTTGTTTAATAACTATATGGCAAAGTTTGGTGTGGAAGTCAGTTACGTTGATTGTTTCGATAATGAC
GCATGGGCAAATGCTGTTCAACCAAATACCAAGGTGATTTATTGCGAAAGCCCTAGTAATCCATTGGCACAGATTTGTGA
TATTGGCTTTTTAAGCCAGCTATGTAAAGCCAATGATATACTCTTGGTCGTTGACAACTGCTTTGCCACCCCAGCTTTGC
AACGTCCGCTTGATTTGGGTGCCGATGTGGTGATTCATTCTGCAACCAAATATTTAGACGGTCAGGGTCGAGTACTTGGT
GGCGCGTTAGTCGGTAGTGATAAGTTGATGCAAGAAGCGTTTACCGTCATACGTTCAGGTGGCATCAGCATGAGTCCGTT
TAATGCTTGGGTGTTTACCAAGGGACTTGAAACGCTTAAATTACGTATGCAAGCGCATTGTAGTAATGCCAATCAAGTGG
CAGAGTTTTTGGTTAATCATCCCAATGTCAGCACCGTACACTTTTCAGGCTTGAGCGACCATCCATCGCATGAGTTGACC
AAGCGTCAGCATCATATGAAAGACGGCTTTGGAGCCATTATGGGTTTTGAAGTGCAGCCATCAGAGACGCGTGATTCAAA
ATCTGCCGCATGGCATGTCATTGATTCAACTCAAATGGTCTCTATCACCAATAATTTGGGTGATGCGAAAACCACGATTA
CCCATCCTGCAACAACGACCCATTTTCGTTTGAGCGCCCAAGAGCGCGCAGAAGCAGGCGTAAAAGACGGTCTTATTCGT
CTGTCGGTCGGTCTAGAAGATGTAGAAGATATCATCAAAGATTTGGCGCGTGGTTTAGACAGTTTGTAA

Upstream 100 bases:

>100_bases
TGCTTGGAATAATTTATCATGATATGATAACGCGAATACGTGATGTTTATAATCAAAAATAAACGCTATTGTAAATAACA
ATCAAGGCAATGGAAACTTT

Downstream 100 bases:

>100_bases
AGATAACGGTATAATTGCTATTAGTATTTTATTTTTTATTATCAAAACCCCCATTATTTATTATTAAAAAACCAAGAGGC
AACTATGAATATCCAAGCAT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSQPQNKDNINGLDANWCNDALDLELDYGMQTIAVRAGQHRTDEGEHSEAIFTTSSYVYSSAADAAGHFDGTKTGNVYSR
HTNPSVRTFERRLAALENGERAVATASGMGAILTMCLAYLKAGNHLLAANQLFGSSIGLFNNYMAKFGVEVSYVDCFDND
AWANAVQPNTKVIYCESPSNPLAQICDIGFLSQLCKANDILLVVDNCFATPALQRPLDLGADVVIHSATKYLDGQGRVLG
GALVGSDKLMQEAFTVIRSGGISMSPFNAWVFTKGLETLKLRMQAHCSNANQVAEFLVNHPNVSTVHFSGLSDHPSHELT
KRQHHMKDGFGAIMGFEVQPSETRDSKSAAWHVIDSTQMVSITNNLGDAKTTITHPATTTHFRLSAQERAEAGVKDGLIR
LSVGLEDVEDIIKDLARGLDSL

Sequences:

>Translated_422_residues
MSQPQNKDNINGLDANWCNDALDLELDYGMQTIAVRAGQHRTDEGEHSEAIFTTSSYVYSSAADAAGHFDGTKTGNVYSR
HTNPSVRTFERRLAALENGERAVATASGMGAILTMCLAYLKAGNHLLAANQLFGSSIGLFNNYMAKFGVEVSYVDCFDND
AWANAVQPNTKVIYCESPSNPLAQICDIGFLSQLCKANDILLVVDNCFATPALQRPLDLGADVVIHSATKYLDGQGRVLG
GALVGSDKLMQEAFTVIRSGGISMSPFNAWVFTKGLETLKLRMQAHCSNANQVAEFLVNHPNVSTVHFSGLSDHPSHELT
KRQHHMKDGFGAIMGFEVQPSETRDSKSAAWHVIDSTQMVSITNNLGDAKTTITHPATTTHFRLSAQERAEAGVKDGLIR
LSVGLEDVEDIIKDLARGLDSL
>Mature_421_residues
SQPQNKDNINGLDANWCNDALDLELDYGMQTIAVRAGQHRTDEGEHSEAIFTTSSYVYSSAADAAGHFDGTKTGNVYSRH
TNPSVRTFERRLAALENGERAVATASGMGAILTMCLAYLKAGNHLLAANQLFGSSIGLFNNYMAKFGVEVSYVDCFDNDA
WANAVQPNTKVIYCESPSNPLAQICDIGFLSQLCKANDILLVVDNCFATPALQRPLDLGADVVIHSATKYLDGQGRVLGG
ALVGSDKLMQEAFTVIRSGGISMSPFNAWVFTKGLETLKLRMQAHCSNANQVAEFLVNHPNVSTVHFSGLSDHPSHELTK
RQHHMKDGFGAIMGFEVQPSETRDSKSAAWHVIDSTQMVSITNNLGDAKTTITHPATTTHFRLSAQERAEAGVKDGLIRL
SVGLEDVEDIIKDLARGLDSL

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=336, Percent_Identity=34.2261904761905, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI299473759, Length=336, Percent_Identity=30.952380952381, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI25453487, Length=335, Percent_Identity=28.955223880597, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=33.5897435897436, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=26.3157894736842, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538055, Length=340, Percent_Identity=34.1176470588235, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=333, Percent_Identity=34.5345345345345, Blast_Score=202, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI71980708, Length=355, Percent_Identity=34.3661971830986, Blast_Score=201, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6319307, Length=394, Percent_Identity=33.502538071066, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=30.5045871559633, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=26.3285024154589, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=266, Percent_Identity=29.6992481203008, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=360, Percent_Identity=25, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=395, Percent_Identity=33.1645569620253, Blast_Score=226, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 45727; Mature: 45596

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPQNKDNINGLDANWCNDALDLELDYGMQTIAVRAGQHRTDEGEHSEAIFTTSSYVYS
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEECHHHHH
SAADAAGHFDGTKTGNVYSRHTNPSVRTFERRLAALENGERAVATASGMGAILTMCLAYL
CCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
KAGNHLLAANQLFGSSIGLFNNYMAKFGVEVSYVDCFDNDAWANAVQPNTKVIYCESPSN
HCCCEEEEHHHHHCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCC
PLAQICDIGFLSQLCKANDILLVVDNCFATPALQRPLDLGADVVIHSATKYLDGQGRVLG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCHHCCCCEEEECHHHHCCCCCCEEE
GALVGSDKLMQEAFTVIRSGGISMSPFNAWVFTKGLETLKLRMQAHCSNANQVAEFLVNH
EEEECCHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHCC
PNVSTVHFSGLSDHPSHELTKRQHHMKDGFGAIMGFEVQPSETRDSKSAAWHVIDSTQMV
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEE
SITNNLGDAKTTITHPATTTHFRLSAQERAEAGVKDGLIRLSVGLEDVEDIIKDLARGLD
EEECCCCCCCEEEECCCCCEEEEECHHHHHHCCHHCCEEEEECCHHHHHHHHHHHHHHHC
SL
CC
>Mature Secondary Structure 
SQPQNKDNINGLDANWCNDALDLELDYGMQTIAVRAGQHRTDEGEHSEAIFTTSSYVYS
CCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEECHHHHH
SAADAAGHFDGTKTGNVYSRHTNPSVRTFERRLAALENGERAVATASGMGAILTMCLAYL
CCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
KAGNHLLAANQLFGSSIGLFNNYMAKFGVEVSYVDCFDNDAWANAVQPNTKVIYCESPSN
HCCCEEEEHHHHHCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCC
PLAQICDIGFLSQLCKANDILLVVDNCFATPALQRPLDLGADVVIHSATKYLDGQGRVLG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCHHCCCCEEEECHHHHCCCCCCEEE
GALVGSDKLMQEAFTVIRSGGISMSPFNAWVFTKGLETLKLRMQAHCSNANQVAEFLVNH
EEEECCHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHCC
PNVSTVHFSGLSDHPSHELTKRQHHMKDGFGAIMGFEVQPSETRDSKSAAWHVIDSTQMV
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEE
SITNNLGDAKTTITHPATTTHFRLSAQERAEAGVKDGLIRLSVGLEDVEDIIKDLARGLD
EEECCCCCCCEEEECCCCCEEEEECHHHHHHCCHHCCEEEEECCHHHHHHHHHHHHHHHC
SL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]