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The map label for this gene is lpdA [H]

Identifier: 93005604

GI number: 93005604

Start: 912619

End: 914154

Strand: Reverse

Name: lpdA [H]

Synonym: Pcryo_0774

Alternate gene names: 93005604

Gene position: 914154-912619 (Counterclockwise)

Preceding gene: 93005605

Following gene: 93005603

Centisome position: 29.88

GC content: 43.42

Gene sequence:

>1536_bases
ATGAATAAACAGCCTGATACCGCCTCTGATTCTTCTGCTCCCTTTGATCATAACATTCATCAAAACGTGACTCGCAAAGT
ATCTGTTGCAGTCATTGGTGCTGGGACTGCTGGTCAAAATGCTTTTCGTCAAGCAAAAAAGTTCTTAGAAGATGTCATTA
TTATCAATGATGGCTTTTGGACAACGACTTGTATTGCGGTAGGTTGCATGCCAAGCAAGCTACTCATTGCAGCCGCTGAC
CGCGCTCATGATACCAATCATTCTGCTGAGTTTGGCGTACATGCCACTGCTCGCATAGATGGCAAACAGGTGATGGAACG
GGTGCGCGCTGAGCGTGATCATTTTGCCAGCTACATCGAAAAACAAGTCGATAGCTGGCCTGAGGATAGTAAGATAGCGG
GACGGGCTTATATTAATAAGCAAGGCTTGATTGAAGTCAATGATGAATTGATCAAAGCAGATAAAATCATTGTCGCCACT
GGCAGCTCGACCTTTATTCCAGATGGCTGGGAAGATAAGCTTGGTGATACTATGCTGACCTCAGATGCTGTCTTTGAGTT
AGCCGACTTACCAAAATCTATGGCTGTGATTGGCGCAGGTGCTATAGGTTTAGAACTTGCCCAAGCTTTCACACGATTGG
GTGTCAAGGTAACGGTATTTAATCGCGCCAAACGCGTTGCTGGTCTACAAGACGAAGACATCAATAACAAAGCTATTGAT
TGCTTAAGTCGTGAGCTGACCATGCACTTGGGCAGCAAAATTACTGATGTCGGTCAAAGATCAGATGAAAATAGCGATAA
AAGCATAAATAAAAATAGCACTGTTGCCTTTATTGATTATGAAGACAGTGCTGGCGAAGTGCAACAATGGCAAGGTGACT
ATGTACTGGTCGCGACTGGTCGACGTAATAATATAGAGGCATTGGGTGTTGAAAACCTAGGCGTTGAGCTTGATGAAAAA
AATCGCCCACAACAGCTGGATAAAAAAACTGGTAAAATCGGTGATTTAGACGTTTATATCGTTGGCGATGCCAATGCTAA
TATTCCCTTGCTGCATGTAGCAAGCGATGAAGGTTTTAGCGCTGGCAGTATGGTCTGCGATAACAAAAAAGACGCTTATA
TTCGTCCACCTGCGACGCCGTTTTCTATCGTCTTTAGTTCACCGCAAATCGTCAATGTTGGTATGTCTTTGCCAGAAATT
CAAGAAGATCCAACACTGGAGCATGTGATTGGGAAAGTCAGCTTCGACAATCAAGGTCGTAGCCGCGTCATGGGTGTTAA
CTGTGGTCTATTGCATATTTATGGCTGCAAAAAAACAGACAGAATCTTGGGTGCAAGTATGGTTGGTCCAGATGCAGAAT
ACATCGGGCATATCTTAGCAATGGCGATTACTAATGACTTGAGTATTAAAGACATGCTTGATACACCTTTTTACCATCCT
ACTATCCTAGAAGGCTTGCGTACCGCCTTGCATGATGTTCAGGATTTGATGGAAATACCGTATCAGTCGCTTGATATTCA
TCAAAACAATTCTTAA

Upstream 100 bases:

>100_bases
AAGCAAAGGATTATACTGAGCGTTTATTAACTACACTTGCACCTTAGTCTTCTAATCTAGCTTACGATAAACCTTAAAAC
TATTTTTATTTGAGAATACT

Downstream 100 bases:

>100_bases
TCAACTGTTAAATGCTCTGTTTTAAAAGTAATGGAGTAGAACTACTGGCGATGACTTAAGAAGACCTAAACTGGATTTGC
CGTCAGGACGTAAGCTACTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MNKQPDTASDSSAPFDHNIHQNVTRKVSVAVIGAGTAGQNAFRQAKKFLEDVIIINDGFWTTTCIAVGCMPSKLLIAAAD
RAHDTNHSAEFGVHATARIDGKQVMERVRAERDHFASYIEKQVDSWPEDSKIAGRAYINKQGLIEVNDELIKADKIIVAT
GSSTFIPDGWEDKLGDTMLTSDAVFELADLPKSMAVIGAGAIGLELAQAFTRLGVKVTVFNRAKRVAGLQDEDINNKAID
CLSRELTMHLGSKITDVGQRSDENSDKSINKNSTVAFIDYEDSAGEVQQWQGDYVLVATGRRNNIEALGVENLGVELDEK
NRPQQLDKKTGKIGDLDVYIVGDANANIPLLHVASDEGFSAGSMVCDNKKDAYIRPPATPFSIVFSSPQIVNVGMSLPEI
QEDPTLEHVIGKVSFDNQGRSRVMGVNCGLLHIYGCKKTDRILGASMVGPDAEYIGHILAMAITNDLSIKDMLDTPFYHP
TILEGLRTALHDVQDLMEIPYQSLDIHQNNS

Sequences:

>Translated_511_residues
MNKQPDTASDSSAPFDHNIHQNVTRKVSVAVIGAGTAGQNAFRQAKKFLEDVIIINDGFWTTTCIAVGCMPSKLLIAAAD
RAHDTNHSAEFGVHATARIDGKQVMERVRAERDHFASYIEKQVDSWPEDSKIAGRAYINKQGLIEVNDELIKADKIIVAT
GSSTFIPDGWEDKLGDTMLTSDAVFELADLPKSMAVIGAGAIGLELAQAFTRLGVKVTVFNRAKRVAGLQDEDINNKAID
CLSRELTMHLGSKITDVGQRSDENSDKSINKNSTVAFIDYEDSAGEVQQWQGDYVLVATGRRNNIEALGVENLGVELDEK
NRPQQLDKKTGKIGDLDVYIVGDANANIPLLHVASDEGFSAGSMVCDNKKDAYIRPPATPFSIVFSSPQIVNVGMSLPEI
QEDPTLEHVIGKVSFDNQGRSRVMGVNCGLLHIYGCKKTDRILGASMVGPDAEYIGHILAMAITNDLSIKDMLDTPFYHP
TILEGLRTALHDVQDLMEIPYQSLDIHQNNS
>Mature_511_residues
MNKQPDTASDSSAPFDHNIHQNVTRKVSVAVIGAGTAGQNAFRQAKKFLEDVIIINDGFWTTTCIAVGCMPSKLLIAAAD
RAHDTNHSAEFGVHATARIDGKQVMERVRAERDHFASYIEKQVDSWPEDSKIAGRAYINKQGLIEVNDELIKADKIIVAT
GSSTFIPDGWEDKLGDTMLTSDAVFELADLPKSMAVIGAGAIGLELAQAFTRLGVKVTVFNRAKRVAGLQDEDINNKAID
CLSRELTMHLGSKITDVGQRSDENSDKSINKNSTVAFIDYEDSAGEVQQWQGDYVLVATGRRNNIEALGVENLGVELDEK
NRPQQLDKKTGKIGDLDVYIVGDANANIPLLHVASDEGFSAGSMVCDNKKDAYIRPPATPFSIVFSSPQIVNVGMSLPEI
QEDPTLEHVIGKVSFDNQGRSRVMGVNCGLLHIYGCKKTDRILGASMVGPDAEYIGHILAMAITNDLSIKDMLDTPFYHP
TILEGLRTALHDVQDLMEIPYQSLDIHQNNS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=29.8538622129436, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI50301238, Length=446, Percent_Identity=23.542600896861, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786307, Length=480, Percent_Identity=26.25, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI87081717, Length=479, Percent_Identity=25.8872651356994, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI87082354, Length=345, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=26.0969976905312, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32565766, Length=477, Percent_Identity=26.8343815513627, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=368, Percent_Identity=24.7282608695652, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983419, Length=368, Percent_Identity=24.7282608695652, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557007, Length=490, Percent_Identity=23.265306122449, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71982272, Length=457, Percent_Identity=22.3194748358862, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=481, Percent_Identity=27.6507276507277, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=25.4817987152034, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6325240, Length=355, Percent_Identity=21.9718309859155, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21358499, Length=484, Percent_Identity=27.2727272727273, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 55767; Mature: 55767

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQPDTASDSSAPFDHNIHQNVTRKVSVAVIGAGTAGQNAFRQAKKFLEDVIIINDGFW
CCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHCEEEEECCCH
TTTCIAVGCMPSKLLIAAADRAHDTNHSAEFGVHATARIDGKQVMERVRAERDHFASYIE
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHH
KQVDSWPEDSKIAGRAYINKQGLIEVNDELIKADKIIVATGSSTFIPDGWEDKLGDTMLT
HHHHCCCCCCCCCCEEEECCCCCEEECHHHCCCCEEEEEECCCCCCCCCCHHHCCCEEEC
SDAVFELADLPKSMAVIGAGAIGLELAQAFTRLGVKVTVFNRAKRVAGLQDEDINNKAID
HHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCHHHHH
CLSRELTMHLGSKITDVGQRSDENSDKSINKNSTVAFIDYEDSAGEVQQWQGDYVLVATG
HHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHCCCCEEEEECC
RRNNIEALGVENLGVELDEKNRPQQLDKKTGKIGDLDVYIVGDANANIPLLHVASDEGFS
CCCCEEEEECCCCCCEECCCCCCHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCC
AGSMVCDNKKDAYIRPPATPFSIVFSSPQIVNVGMSLPEIQEDPTLEHVIGKVSFDNQGR
CCCEEECCCCCCEECCCCCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHEECCCCCCCC
SRVMGVNCGLLHIYGCKKTDRILGASMVGPDAEYIGHILAMAITNDLSIKDMLDTPFYHP
CEEEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCH
TILEGLRTALHDVQDLMEIPYQSLDIHQNNS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNKQPDTASDSSAPFDHNIHQNVTRKVSVAVIGAGTAGQNAFRQAKKFLEDVIIINDGFW
CCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHCEEEEECCCH
TTTCIAVGCMPSKLLIAAADRAHDTNHSAEFGVHATARIDGKQVMERVRAERDHFASYIE
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHH
KQVDSWPEDSKIAGRAYINKQGLIEVNDELIKADKIIVATGSSTFIPDGWEDKLGDTMLT
HHHHCCCCCCCCCCEEEECCCCCEEECHHHCCCCEEEEEECCCCCCCCCCHHHCCCEEEC
SDAVFELADLPKSMAVIGAGAIGLELAQAFTRLGVKVTVFNRAKRVAGLQDEDINNKAID
HHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCHHHHH
CLSRELTMHLGSKITDVGQRSDENSDKSINKNSTVAFIDYEDSAGEVQQWQGDYVLVATG
HHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHCCCCEEEEECC
RRNNIEALGVENLGVELDEKNRPQQLDKKTGKIGDLDVYIVGDANANIPLLHVASDEGFS
CCCCEEEEECCCCCCEECCCCCCHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCC
AGSMVCDNKKDAYIRPPATPFSIVFSSPQIVNVGMSLPEIQEDPTLEHVIGKVSFDNQGR
CCCEEECCCCCCEECCCCCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHEECCCCCCCC
SRVMGVNCGLLHIYGCKKTDRILGASMVGPDAEYIGHILAMAITNDLSIKDMLDTPFYHP
CEEEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCH
TILEGLRTALHDVQDLMEIPYQSLDIHQNNS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]