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The map label for this gene is paaG [C]

Identifier: 93005580

GI number: 93005580

Start: 882366

End: 883163

Strand: Reverse

Name: paaG [C]

Synonym: Pcryo_0750

Alternate gene names: 93005580

Gene position: 883163-882366 (Counterclockwise)

Preceding gene: 93005582

Following gene: 93005575

Centisome position: 28.86

GC content: 42.98

Gene sequence:

>798_bases
ATGTCACAAGCTCTCGTCGAATACCAAGCCGAAAACCATATTGCTACCATCACAATGAACGATCCTAAAACCTTGAACGC
CTTTAGCACGGTACTCAAAGATGCCATGATGGCAGCATTGACCAAAGCCGATGAAGATGAGCAAGTACGAGTCATTGTCT
TACAGGGCGCCGGCAATAACTTCTCAAGTGGCGGGCATATCGGTGAGATGTTAGAAAACGGTATGGAAAGCGAGGCATTA
TCAGATAAGCTAAACTTTATGGTTAGTGAAGTCGCTGAGATTAGCTTAAAGCTGCGTAATATTCATAAGCCTATTATCGC
CAAATTAGAAGGTGCCGTTGCTGGTGCTGGCATGAACTTGGCGCTGACTTGTGACTTTCGTATTGCCGCTGAAAATGCCA
AATTTGTACAAGCTTTTGTCAATATTGGTCTGATTCCAGATGCTGGTGGTATTTACTTATTGAATCAATTGATTGGCGTC
GCAAAAACGAATGAAATGGTCATGCTTGGCGATAAGCTCAAAGCGGAAGATATGGCACGCTTAAACTTAGTCAACGATGT
GGTTAGTAACGATGAGCTTGATGCGAGCGTACTGGCATTGGCAACGCGTCTGAGCAATCTACCCGGCAAAGCATTGGCAT
CGATGAAGCACATGATTAATACTCATGCCTATATGGGTCTTAATGAAGCATTGGATATGGAAGTAGAACAACAAACGATG
CTTGCTAAAACCGATGATTTTAAAGAAGGTATTACCGCATTTATGGAAAAGCGTAAGCCCGTTTATCAAGGCAAATAA

Upstream 100 bases:

>100_bases
AGTAGTGCTTTATAACCTGTATATTTCTTTTATTCAGGCATGACTATACCAGTATTTTATTGCAACCATTATCTAGAACA
TCATCTTAAGGATATCCAAA

Downstream 100 bases:

>100_bases
AAACTGATAACACTGTTCTACTAAATATCATTCGAAAGGCTGCCATTAGGTAGCCTTTTATTTTGGATAAATTATATAAT
TAATGGTCAAAATTATTCCC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSQALVEYQAENHIATITMNDPKTLNAFSTVLKDAMMAALTKADEDEQVRVIVLQGAGNNFSSGGHIGEMLENGMESEAL
SDKLNFMVSEVAEISLKLRNIHKPIIAKLEGAVAGAGMNLALTCDFRIAAENAKFVQAFVNIGLIPDAGGIYLLNQLIGV
AKTNEMVMLGDKLKAEDMARLNLVNDVVSNDELDASVLALATRLSNLPGKALASMKHMINTHAYMGLNEALDMEVEQQTM
LAKTDDFKEGITAFMEKRKPVYQGK

Sequences:

>Translated_265_residues
MSQALVEYQAENHIATITMNDPKTLNAFSTVLKDAMMAALTKADEDEQVRVIVLQGAGNNFSSGGHIGEMLENGMESEAL
SDKLNFMVSEVAEISLKLRNIHKPIIAKLEGAVAGAGMNLALTCDFRIAAENAKFVQAFVNIGLIPDAGGIYLLNQLIGV
AKTNEMVMLGDKLKAEDMARLNLVNDVVSNDELDASVLALATRLSNLPGKALASMKHMINTHAYMGLNEALDMEVEQQTM
LAKTDDFKEGITAFMEKRKPVYQGK
>Mature_264_residues
SQALVEYQAENHIATITMNDPKTLNAFSTVLKDAMMAALTKADEDEQVRVIVLQGAGNNFSSGGHIGEMLENGMESEALS
DKLNFMVSEVAEISLKLRNIHKPIIAKLEGAVAGAGMNLALTCDFRIAAENAKFVQAFVNIGLIPDAGGIYLLNQLIGVA
KTNEMVMLGDKLKAEDMARLNLVNDVVSNDELDASVLALATRLSNLPGKALASMKHMINTHAYMGLNEALDMEVEQQTML
AKTDDFKEGITAFMEKRKPVYQGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=34.1269841269841, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI68989263, Length=206, Percent_Identity=31.0679611650485, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=24.8995983935743, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI70995211, Length=199, Percent_Identity=29.1457286432161, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=29.2817679558011, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=29.2817679558011, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI20127408, Length=192, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=23.1441048034935, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=23.4042553191489, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI4757968, Length=234, Percent_Identity=24.3589743589744, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI49355787, Length=234, Percent_Identity=24.3589743589744, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI4757966, Length=234, Percent_Identity=23.9316239316239, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI51538581, Length=234, Percent_Identity=23.9316239316239, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI25453481, Length=234, Percent_Identity=23.9316239316239, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI51538573, Length=234, Percent_Identity=23.9316239316239, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=33.0798479087452, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=30.5660377358491, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=33.59375, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI87082183, Length=273, Percent_Identity=28.9377289377289, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=29.5019157088123, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=30.2439024390244, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=32.9457364341085, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=30.1960784313725, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=246, Percent_Identity=28.0487804878049, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=256, Percent_Identity=26.5625, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=23.3870967741935, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=22.1374045801527, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=156, Percent_Identity=27.5641025641026, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=257, Percent_Identity=22.9571984435798, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=187, Percent_Identity=28.8770053475936, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=187, Percent_Identity=28.8770053475936, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=187, Percent_Identity=28.8770053475936, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=26.7379679144385, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=197, Percent_Identity=24.8730964467005, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=33.4677419354839, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=33.4677419354839, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24654903, Length=254, Percent_Identity=25.5905511811024, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653139, Length=271, Percent_Identity=28.0442804428044, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=24.7104247104247, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=25.968992248062, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24650670, Length=255, Percent_Identity=26.2745098039216, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571730, Length=144, Percent_Identity=29.8611111111111, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28571729, Length=144, Percent_Identity=29.8611111111111, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28735; Mature: 28603

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQALVEYQAENHIATITMNDPKTLNAFSTVLKDAMMAALTKADEDEQVRVIVLQGAGNN
CCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCC
FSSGGHIGEMLENGMESEALSDKLNFMVSEVAEISLKLRNIHKPIIAKLEGAVAGAGMNL
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ALTCDFRIAAENAKFVQAFVNIGLIPDAGGIYLLNQLIGVAKTNEMVMLGDKLKAEDMAR
EEEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
LNLVNDVVSNDELDASVLALATRLSNLPGKALASMKHMINTHAYMGLNEALDMEVEQQTM
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
LAKTDDFKEGITAFMEKRKPVYQGK
HHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SQALVEYQAENHIATITMNDPKTLNAFSTVLKDAMMAALTKADEDEQVRVIVLQGAGNN
CCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCC
FSSGGHIGEMLENGMESEALSDKLNFMVSEVAEISLKLRNIHKPIIAKLEGAVAGAGMNL
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ALTCDFRIAAENAKFVQAFVNIGLIPDAGGIYLLNQLIGVAKTNEMVMLGDKLKAEDMAR
EEEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
LNLVNDVVSNDELDASVLALATRLSNLPGKALASMKHMINTHAYMGLNEALDMEVEQQTM
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
LAKTDDFKEGITAFMEKRKPVYQGK
HHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA