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The map label for this gene is mvaB [H]

Identifier: 93005174

GI number: 93005174

Start: 416609

End: 417529

Strand: Direct

Name: mvaB [H]

Synonym: Pcryo_0343

Alternate gene names: 93005174

Gene position: 416609-417529 (Clockwise)

Preceding gene: 93005173

Following gene: 93005175

Centisome position: 13.62

GC content: 48.21

Gene sequence:

>921_bases
ATGAGTATCTCGTACCCAAAACACGTCACCATCGTCGATGTCAGTCCACGTGATGGCTTGCAAAATGAAGCCATGACCGT
GCCGACTGCGGTTAAGCAAAGGCTTATAAGTGACTTGATTGCCGCAGGGGTTAAAAAGCTCGAGGCTACTAGCTTTGTCT
CGCCAAAGTGGGTACCGCAAATGGGCGACAATAGCACGCTGTTAGAGGCACTTGCAGCGACACGCCACAGTGATGTGAGC
TATTCGGTATTGGTTCCAAACATGCGCGGTTTTGAAAATGCTATCGTGCATCGTCCTGATGAGATCGTCATCTTTGGCTC
AGCAAGCGAAACCTTTAGCCAAAAAAATATCAATTGTAGTATTGATGAAAGTATTGAGCGTTTTGCGCCCGTTGCCGCTG
CTGCAAAAGCGCAAGGTATCAAAGTACGCGGCGTTATCTCTTGCACGGTCGGTTGCCCCTATGAAGGCGAAATTGACCCA
CGCCAAGTGGCTTATGTCACCAAGCGTTTGGTTGAGATAGGCTCGGAGCAGATTGGCATTGCTGATACCATCGGCGTTGG
TACGCCGCTCAAAGTACAGCGTGCGTTACAAGCAGCGTTAGAGTACGCTGATATCAGTATGCTATCGGGACATTTTCACG
ATACTTACGGTCAAGCCCTCAGCAACACATTAGCGGCACTACAAATAGGTGTTAGCGAATTTGATACCTCCGTCGCTGGA
CTTGGTGGTTGTCCTTATGCCAAAGGCGCAACGGGCAATGTCGCTACTGAAGATGTGGTTTATATGCTGCATGGTATGGG
TATCAGTACTGGTATTGATTTGGATAAATTGGTCGTGGCAGGTGAGCGTATCAGTGAATTTTTAGGTCGTCCTAATGGCT
CAAACGTGGCACGTGCTTTAATTAACAAACGTCAGTCTTAA

Upstream 100 bases:

>100_bases
TGGTGGCAGAATTGTTATTTGGCGCGGGTGACTTGGTTGCTGATGGTGATGAGCTGCTGCGTATTGATAGCCAAGAGAAT
CAGTAAAAAGGACAATCTGT

Downstream 100 bases:

>100_bases
CTAATTTCATTAAGAATATAGTTAGTCATAAGCTAAATGAATAGATAGATAGGTTTATGGCTGGGACAAATTTTTTGAAG
CCTCTGGAGTGACGAAGTCG

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSISYPKHVTIVDVSPRDGLQNEAMTVPTAVKQRLISDLIAAGVKKLEATSFVSPKWVPQMGDNSTLLEALAATRHSDVS
YSVLVPNMRGFENAIVHRPDEIVIFGSASETFSQKNINCSIDESIERFAPVAAAAKAQGIKVRGVISCTVGCPYEGEIDP
RQVAYVTKRLVEIGSEQIGIADTIGVGTPLKVQRALQAALEYADISMLSGHFHDTYGQALSNTLAALQIGVSEFDTSVAG
LGGCPYAKGATGNVATEDVVYMLHGMGISTGIDLDKLVVAGERISEFLGRPNGSNVARALINKRQS

Sequences:

>Translated_306_residues
MSISYPKHVTIVDVSPRDGLQNEAMTVPTAVKQRLISDLIAAGVKKLEATSFVSPKWVPQMGDNSTLLEALAATRHSDVS
YSVLVPNMRGFENAIVHRPDEIVIFGSASETFSQKNINCSIDESIERFAPVAAAAKAQGIKVRGVISCTVGCPYEGEIDP
RQVAYVTKRLVEIGSEQIGIADTIGVGTPLKVQRALQAALEYADISMLSGHFHDTYGQALSNTLAALQIGVSEFDTSVAG
LGGCPYAKGATGNVATEDVVYMLHGMGISTGIDLDKLVVAGERISEFLGRPNGSNVARALINKRQS
>Mature_305_residues
SISYPKHVTIVDVSPRDGLQNEAMTVPTAVKQRLISDLIAAGVKKLEATSFVSPKWVPQMGDNSTLLEALAATRHSDVSY
SVLVPNMRGFENAIVHRPDEIVIFGSASETFSQKNINCSIDESIERFAPVAAAAKAQGIKVRGVISCTVGCPYEGEIDPR
QVAYVTKRLVEIGSEQIGIADTIGVGTPLKVQRALQAALEYADISMLSGHFHDTYGQALSNTLAALQIGVSEFDTSVAGL
GGCPYAKGATGNVATEDVVYMLHGMGISTGIDLDKLVVAGERISEFLGRPNGSNVARALINKRQS

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=296, Percent_Identity=54.3918918918919, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI109150427, Length=297, Percent_Identity=48.8215488215488, Blast_Score=306, Evalue=1e-83,
Organism=Homo sapiens, GI109150422, Length=297, Percent_Identity=48.8215488215488, Blast_Score=306, Evalue=1e-83,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=55.8823529411765, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17510563, Length=295, Percent_Identity=45.4237288135593, Blast_Score=268, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24582381, Length=296, Percent_Identity=51.6891891891892, Blast_Score=314, Evalue=6e-86,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32534; Mature: 32403

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISYPKHVTIVDVSPRDGLQNEAMTVPTAVKQRLISDLIAAGVKKLEATSFVSPKWVPQ
CCCCCCCEEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
MGDNSTLLEALAATRHSDVSYSVLVPNMRGFENAIVHRPDEIVIFGSASETFSQKNINCS
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHEECCCEEEEECCCHHHHHHCCCCCC
IDESIERFAPVAAAAKAQGIKVRGVISCTVGCPYEGEIDPRQVAYVTKRLVEIGSEQIGI
HHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
ADTIGVGTPLKVQRALQAALEYADISMLSGHFHDTYGQALSNTLAALQIGVSEFDTSVAG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGGCPYAKGATGNVATEDVVYMLHGMGISTGIDLDKLVVAGERISEFLGRPNGSNVARAL
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
INKRQS
HHCCCC
>Mature Secondary Structure 
SISYPKHVTIVDVSPRDGLQNEAMTVPTAVKQRLISDLIAAGVKKLEATSFVSPKWVPQ
CCCCCCEEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
MGDNSTLLEALAATRHSDVSYSVLVPNMRGFENAIVHRPDEIVIFGSASETFSQKNINCS
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHEECCCEEEEECCCHHHHHHCCCCCC
IDESIERFAPVAAAAKAQGIKVRGVISCTVGCPYEGEIDPRQVAYVTKRLVEIGSEQIGI
HHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
ADTIGVGTPLKVQRALQAALEYADISMLSGHFHDTYGQALSNTLAALQIGVSEFDTSVAG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGGCPYAKGATGNVATEDVVYMLHGMGISTGIDLDKLVVAGERISEFLGRPNGSNVARAL
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
INKRQS
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]