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The map label for this gene is yjeA [H]

Identifier: 93005130

GI number: 93005130

Start: 360057

End: 361079

Strand: Direct

Name: yjeA [H]

Synonym: Pcryo_0299

Alternate gene names: 93005130

Gene position: 360057-361079 (Clockwise)

Preceding gene: 93005129

Following gene: 93005137

Centisome position: 11.77

GC content: 44.57

Gene sequence:

>1023_bases
ATGAGTAAAGCCAATAGTCAGCCTTTATCTAATCTAAGCTATGCCCCAACGATGACCTTTGCAATGGCGCAGCAGCGTGC
AAAATTAATGAGTGTCATTCGTCAGTTTTTTGCTATGCGTCAAGTATTAGAAGTGCAGACGCCGCTATTATCGCAAGCAG
GCAATACCGATACCTTTTTGCAATCCGTAGCCGCACAGGTCACGTATCAAGACAAACCTTGTACTTATTATCTGCATACG
TCACCTGAGTTTGCCATGAAGCGTTTGCTTGCCAGCTGGCAGGTGCCCATTTATCAAATCTGTCCGGTCTTTCGAGATAA
TGAGATCGGGGCGCGCCACAATATCGAATTCACCATGCTTGAATGGTATCAGCCAAATTATAGTCTGGATGGTCTGGCAG
CAGAGCTTGGTGAATTATTAGAGATGGTCTATGGTCATCCGATAATCATGAGTCATTATCGCTACGTTGATGCTTTTATG
GATTTTGTTGGTATTCACCCGCTGACCGCCAGTCTAGCAGCATTACAAGCCGTGGCAGAAGACAAAGATCTGATAGGATT
TGATTTTAATAGTGCAGAGAGCTGCGATAGCGAACAAGACCGTCGTCAAAGCTGGCTGGATTTACTATTTAGCCATGCTG
TTGAGCCAAATTTGGGTCACGATTTGCCGACATTGATTATTGAATATCCGCCTGCAACAGCTGCTTTAGCAAAAACCGCT
ATTGATAAAGAGGGGAATAAAGTCGCCAAACGTTTTGAGCTGTATATCAAAGGAATCGAGATTGCCAACGCTTATGATGA
GTTAGCAGACGGGCAGGCGTTAAGGGAACGGTTTGAACAGGACAACAAATTGAGAGGGCGTCATAACTTGCCACAAATGC
CTATTGATGAGCACTTAATAGCAGCGTCTGATGATTTGATACCTTGTAGCGGGATTGCTGTCGGTTTAGATAGGCTGCTG
ATGGTATTGACAGATGCAACGAGCTTAGAAGAAGTTATCTCATTTCCTAGTGGTTCGGCTTAG

Upstream 100 bases:

>100_bases
ACGCCGTGATTACCATCTGCGCCGTGAATGGCAAGCACAAACAATCAGTGTGACTATTTAATTATCTGTTTTTACGATTT
ATGAGGCACTGTCACCAGTC

Downstream 100 bases:

>100_bases
TCGCTCTTTGATAGATTCAACATAGTCGCCACTTATTTAACAAGACTATTAGAAAAAAGCGAGTAAGCCTTTTGACTTAC
TCGCTTTTTTGTCTGTCCTT

Product: lysine--tRNA ligase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSKANSQPLSNLSYAPTMTFAMAQQRAKLMSVIRQFFAMRQVLEVQTPLLSQAGNTDTFLQSVAAQVTYQDKPCTYYLHT
SPEFAMKRLLASWQVPIYQICPVFRDNEIGARHNIEFTMLEWYQPNYSLDGLAAELGELLEMVYGHPIIMSHYRYVDAFM
DFVGIHPLTASLAALQAVAEDKDLIGFDFNSAESCDSEQDRRQSWLDLLFSHAVEPNLGHDLPTLIIEYPPATAALAKTA
IDKEGNKVAKRFELYIKGIEIANAYDELADGQALRERFEQDNKLRGRHNLPQMPIDEHLIAASDDLIPCSGIAVGLDRLL
MVLTDATSLEEVISFPSGSA

Sequences:

>Translated_340_residues
MSKANSQPLSNLSYAPTMTFAMAQQRAKLMSVIRQFFAMRQVLEVQTPLLSQAGNTDTFLQSVAAQVTYQDKPCTYYLHT
SPEFAMKRLLASWQVPIYQICPVFRDNEIGARHNIEFTMLEWYQPNYSLDGLAAELGELLEMVYGHPIIMSHYRYVDAFM
DFVGIHPLTASLAALQAVAEDKDLIGFDFNSAESCDSEQDRRQSWLDLLFSHAVEPNLGHDLPTLIIEYPPATAALAKTA
IDKEGNKVAKRFELYIKGIEIANAYDELADGQALRERFEQDNKLRGRHNLPQMPIDEHLIAASDDLIPCSGIAVGLDRLL
MVLTDATSLEEVISFPSGSA
>Mature_339_residues
SKANSQPLSNLSYAPTMTFAMAQQRAKLMSVIRQFFAMRQVLEVQTPLLSQAGNTDTFLQSVAAQVTYQDKPCTYYLHTS
PEFAMKRLLASWQVPIYQICPVFRDNEIGARHNIEFTMLEWYQPNYSLDGLAAELGELLEMVYGHPIIMSHYRYVDAFMD
FVGIHPLTASLAALQAVAEDKDLIGFDFNSAESCDSEQDRRQSWLDLLFSHAVEPNLGHDLPTLIIEYPPATAALAKTAI
DKEGNKVAKRFELYIKGIEIANAYDELADGQALRERFEQDNKLRGRHNLPQMPIDEHLIAASDDLIPCSGIAVGLDRLLM
VLTDATSLEEVISFPSGSA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=344, Percent_Identity=27.3255813953488, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI5031815, Length=344, Percent_Identity=27.3255813953488, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=42.638036809816, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790571, Length=322, Percent_Identity=26.7080745341615, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789256, Length=325, Percent_Identity=28.3076923076923, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71994340, Length=337, Percent_Identity=27.893175074184, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=337, Percent_Identity=27.893175074184, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=337, Percent_Identity=27.893175074184, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=334, Percent_Identity=27.2455089820359, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324256, Length=349, Percent_Identity=26.647564469914, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640849, Length=335, Percent_Identity=27.7611940298507, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640851, Length=335, Percent_Identity=27.7611940298507, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 38072; Mature: 37941

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKANSQPLSNLSYAPTMTFAMAQQRAKLMSVIRQFFAMRQVLEVQTPLLSQAGNTDTFL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QSVAAQVTYQDKPCTYYLHTSPEFAMKRLLASWQVPIYQICPVFRDNEIGARHNIEFTML
HHHHHHHEECCCCEEEEEECCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEE
EWYQPNYSLDGLAAELGELLEMVYGHPIIMSHYRYVDAFMDFVGIHPLTASLAALQAVAE
EEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
DKDLIGFDFNSAESCDSEQDRRQSWLDLLFSHAVEPNLGHDLPTLIIEYPPATAALAKTA
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHH
IDKEGNKVAKRFELYIKGIEIANAYDELADGQALRERFEQDNKLRGRHNLPQMPIDEHLI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
AASDDLIPCSGIAVGLDRLLMVLTDATSLEEVISFPSGSA
HCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKANSQPLSNLSYAPTMTFAMAQQRAKLMSVIRQFFAMRQVLEVQTPLLSQAGNTDTFL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QSVAAQVTYQDKPCTYYLHTSPEFAMKRLLASWQVPIYQICPVFRDNEIGARHNIEFTML
HHHHHHHEECCCCEEEEEECCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEE
EWYQPNYSLDGLAAELGELLEMVYGHPIIMSHYRYVDAFMDFVGIHPLTASLAALQAVAE
EEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
DKDLIGFDFNSAESCDSEQDRRQSWLDLLFSHAVEPNLGHDLPTLIIEYPPATAALAKTA
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHH
IDKEGNKVAKRFELYIKGIEIANAYDELADGQALRERFEQDNKLRGRHNLPQMPIDEHLI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
AASDDLIPCSGIAVGLDRLLMVLTDATSLEEVISFPSGSA
HCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA