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The map label for this gene is lplA

Identifier: 93005028

GI number: 93005028

Start: 232528

End: 233547

Strand: Reverse

Name: lplA

Synonym: Pcryo_0197

Alternate gene names: 93005028

Gene position: 233547-232528 (Counterclockwise)

Preceding gene: 93005031

Following gene: 93005024

Centisome position: 7.63

GC content: 45.29

Gene sequence:

>1020_bases
ATGAAACTGCGCATCTTAAAGTCTGCCGTGACTAACCCTTGGTTTAACCTCGCGACCGAAGATTGGATATTTAATACCCT
CAATCCCGACTCGCACACGCTATTTTTATGGCGCAATAGCGAGACCGTGGTCATTGGGCGCTCGCAAAACCCATGGGTGG
AATGCAAAATCGATAAGATGGAAGCCGACGATGTGTTTTTAGCAAGACGTCAGAGTGGCGGTGGTGCGGTATTTCATGAT
TTAGGCAATACCAACTTTACCTTTTTATCACCCAAAGACGATTACGACCAAGCAGCGAACTTTACCATTATTATCAATGC
GCTAAAGAAGTTGGGCATAGACGCGGATTTATCTGGGCGTAACGACATGCAAGTCGGTGATAAGAAGATATCAGGTAGCG
CCTTTAAACATACAGCAGATCGCAGCTTTCATCATGGCACATTACTAGTCAATGCCAATATGCAAAAGCTGGGCGATTAT
CTCAATCCGCATCCGCTAAAGCTGAAAGCCAAAGGCATTAAGTCCGTCCGTGCGCGCGTTGCTAATTTGGTTGAGTTTAA
TGAAGACATCAATCACGAGACGTTATCTGATGCAATTATCGAAGCGTTTCGCGAATACTATCGCGACACGGATTATGGCG
ACACCGCGCCAGTCGAAGAATTGGATGAAGCCAGTCTCGCCAAGCAACCTAACCTTAATAAATATTATCAACAAATGGCA
GATTGGGATTGGCGCTTTGGCAAGACGCCTGAGTTCACCCATCACATTGAGACGCGCTTTAATTGGGGCATTATTGATTT
GCACCTTGATGTGAAGCAAGCAGCGATACGCGAAGTGGTCATCTTCTCTGATGCGCTAAACGTTGAGTTGATTGATCTGT
TAAAAGAATCACTGGCAGATGTTAAGTACGACAAGCATGATATTAAAGCTAAGTTTGATGAGCTAAACAGAGCGCATCCT
GAATTGGCAGCGCAGATTGACGATGTGTCGGAGTGGCTAATTGGAGAGATGGAAGGTTAA

Upstream 100 bases:

>100_bases
TACCACACAGCCATTGAATTCCAAACTCGCATCGGCAACAATGACAGTATCTATTTATGACACTCAATCTATAAAAACTA
TTCGAAAAAAAGGATAAATA

Downstream 100 bases:

>100_bases
AGCCTTGCGGTTGCAAGTGAAATAAATATTTGCAACCGCTCCTATTTTTATTCTAAGCCATCCAAAATCATTTTCACCTC
CGTCACATATTGACCCAAAT

Product: lipoyltransferase and lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKLRILKSAVTNPWFNLATEDWIFNTLNPDSHTLFLWRNSETVVIGRSQNPWVECKIDKMEADDVFLARRQSGGGAVFHD
LGNTNFTFLSPKDDYDQAANFTIIINALKKLGIDADLSGRNDMQVGDKKISGSAFKHTADRSFHHGTLLVNANMQKLGDY
LNPHPLKLKAKGIKSVRARVANLVEFNEDINHETLSDAIIEAFREYYRDTDYGDTAPVEELDEASLAKQPNLNKYYQQMA
DWDWRFGKTPEFTHHIETRFNWGIIDLHLDVKQAAIREVVIFSDALNVELIDLLKESLADVKYDKHDIKAKFDELNRAHP
ELAAQIDDVSEWLIGEMEG

Sequences:

>Translated_339_residues
MKLRILKSAVTNPWFNLATEDWIFNTLNPDSHTLFLWRNSETVVIGRSQNPWVECKIDKMEADDVFLARRQSGGGAVFHD
LGNTNFTFLSPKDDYDQAANFTIIINALKKLGIDADLSGRNDMQVGDKKISGSAFKHTADRSFHHGTLLVNANMQKLGDY
LNPHPLKLKAKGIKSVRARVANLVEFNEDINHETLSDAIIEAFREYYRDTDYGDTAPVEELDEASLAKQPNLNKYYQQMA
DWDWRFGKTPEFTHHIETRFNWGIIDLHLDVKQAAIREVVIFSDALNVELIDLLKESLADVKYDKHDIKAKFDELNRAHP
ELAAQIDDVSEWLIGEMEG
>Mature_339_residues
MKLRILKSAVTNPWFNLATEDWIFNTLNPDSHTLFLWRNSETVVIGRSQNPWVECKIDKMEADDVFLARRQSGGGAVFHD
LGNTNFTFLSPKDDYDQAANFTIIINALKKLGIDADLSGRNDMQVGDKKISGSAFKHTADRSFHHGTLLVNANMQKLGDY
LNPHPLKLKAKGIKSVRARVANLVEFNEDINHETLSDAIIEAFREYYRDTDYGDTAPVEELDEASLAKQPNLNKYYQQMA
DWDWRFGKTPEFTHHIETRFNWGIIDLHLDVKQAAIREVVIFSDALNVELIDLLKESLADVKYDKHDIKAKFDELNRAHP
ELAAQIDDVSEWLIGEMEG

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family

Homologues:

Organism=Homo sapiens, GI7706252, Length=329, Percent_Identity=31.9148936170213, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI21729884, Length=329, Percent_Identity=31.9148936170213, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI21729882, Length=329, Percent_Identity=31.9148936170213, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI21729880, Length=329, Percent_Identity=31.9148936170213, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI21729878, Length=329, Percent_Identity=31.9148936170213, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI1790846, Length=337, Percent_Identity=44.8071216617211, Blast_Score=313, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI71981909, Length=270, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322415, Length=189, Percent_Identity=32.8042328042328, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28573464, Length=296, Percent_Identity=35.1351351351351, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI221330307, Length=177, Percent_Identity=29.3785310734463, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPLA_PSYCK (Q1QEC2)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579465.1
- ProteinModelPortal:   Q1QEC2
- SMR:   Q1QEC2
- STRING:   Q1QEC2
- GeneID:   4035677
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0197
- NMPDR:   fig|335284.3.peg.806
- eggNOG:   COG0095
- HOGENOM:   HBG715377
- OMA:   DWIHDHV
- PhylomeDB:   Q1QEC2
- ProtClustDB:   CLSK846336
- BioCyc:   PCRY335284:PCRYO_0197-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01602
- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562
- Gene3D:   G3DSA:3.30.390.50
- TIGRFAMs:   TIGR00545

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C

EC number: =2.7.7.63

Molecular weight: Translated: 38722; Mature: 38722

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: BINDING 70-70 BINDING 129-129 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRILKSAVTNPWFNLATEDWIFNTLNPDSHTLFLWRNSETVVIGRSQNPWVECKIDKM
CCEEEEHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCEEEEEEECC
EADDVFLARRQSGGGAVFHDLGNTNFTFLSPKDDYDQAANFTIIINALKKLGIDADLSGR
CCCCEEEEEECCCCCEEEEECCCCCEEEECCCCCHHHHCCHHHEEEHHHHHCCCCCCCCC
NDMQVGDKKISGSAFKHTADRSFHHGTLLVNANMQKLGDYLNPHPLKLKAKGIKSVRARV
CCCCCCCEECCCCHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCEEEEHHHHHHHHHHH
ANLVEFNEDINHETLSDAIIEAFREYYRDTDYGDTAPVEELDEASLAKQPNLNKYYQQMA
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
DWDWRFGKTPEFTHHIETRFNWGIIDLHLDVKQAAIREVVIFSDALNVELIDLLKESLAD
CCCCCCCCCCCHHHHHHCEECCCEEEEEECHHHHHHHHHEEECCCCCHHHHHHHHHHHHH
VKYDKHDIKAKFDELNRAHPELAAQIDDVSEWLIGEMEG
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLRILKSAVTNPWFNLATEDWIFNTLNPDSHTLFLWRNSETVVIGRSQNPWVECKIDKM
CCEEEEHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCEEEEEEECC
EADDVFLARRQSGGGAVFHDLGNTNFTFLSPKDDYDQAANFTIIINALKKLGIDADLSGR
CCCCEEEEEECCCCCEEEEECCCCCEEEECCCCCHHHHCCHHHEEEHHHHHCCCCCCCCC
NDMQVGDKKISGSAFKHTADRSFHHGTLLVNANMQKLGDYLNPHPLKLKAKGIKSVRARV
CCCCCCCEECCCCHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCEEEEHHHHHHHHHHH
ANLVEFNEDINHETLSDAIIEAFREYYRDTDYGDTAPVEELDEASLAKQPNLNKYYQQMA
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
DWDWRFGKTPEFTHHIETRFNWGIIDLHLDVKQAAIREVVIFSDALNVELIDLLKESLAD
CCCCCCCCCCCHHHHHHCEECCCEEEEEECHHHHHHHHHEEECCCCCHHHHHHHHHHHHH
VKYDKHDIKAKFDELNRAHPELAAQIDDVSEWLIGEMEG
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA