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The map label for this gene is thiED [H]

Identifier: 93005010

GI number: 93005010

Start: 209827

End: 210621

Strand: Reverse

Name: thiED [H]

Synonym: Pcryo_0179

Alternate gene names: 93005010

Gene position: 210621-209827 (Counterclockwise)

Preceding gene: 93005012

Following gene: 93005007

Centisome position: 6.88

GC content: 48.81

Gene sequence:

>795_bases
ATGCGACCAGTCGTATTGTGCTTTTCAGGCTTAGACCCCTCAGGCGGTGCTGGATTACAAGCGGATATAGAAGCCATCGG
TCAAGCAGGCGCTCATGCTGCTATCGCTTGTACGGCTATTACCATACAAAACTCGCAACAGGTATTTGGCTTTGAAGCAT
GTACGGCAAGTTTGGTCAACTCTCAAGCGGTAGCAGTGCTCGATGATTTGCCTGTTGCCGTCATCAAGTCAGGCATGCTT
GGTACGACTGACAATATTGCTATGCTAAGCCAATTATTTGCTGACGAGATTATTGTTAAGGGTACTCCTTTTGTATTGGA
CCCAGTATTGGTCGCCAACAGTGGTGGTAGCTTAGGCGATGAGCAGACGCTAGTCAGCGCCTTTAAACAGCTGCTACCCT
ATGCCACCTTGATTACCCCTAACACTCATGAGCTGCGCGCCTTGAGTGGTGAACAAGACTTACATATAGGTGCTCAGAAG
TTATGTGCACAAGGCACTCATGCAGTACTGGTCAAAACCTCACACGACTTCGATAGCGGTGATATTGAGCAGTATTTATA
CATAAAAGGCGAGATGGTACATAAAAGTATCTTGCCACGCCTAAAAGGCGAATTTCACGGCTCTGGCTGTTCACTAGCGA
GCTTTATCGCTGGGCGCTTAGCGATGGGTGATGCGCTTATCGAGGCAGTAAAAGCGGCGGATAGCTGGATTACCAAAACG
CTGCGTGCCGCTGATGCACCGCACCCTGAGGATGCTAAAGCGCAATTGATACCCAACCGCTTTATGCGCTTTTAG

Upstream 100 bases:

>100_bases
CTTTTTGCATAATTATCGATTTATCGGTCTTTATGGCTTTTTTGACAGTAGATGGCTATAGTATTTACCACTTATTATAA
AAGATATCAAAGGATGTGTT

Downstream 100 bases:

>100_bases
CACTTAGTATTTATCATATAATTTTATCTAAATAACGAACATAAAAAAGGCACTCTATCTTGTATAGAGCGCCTTTTTTA
TTTGAAATTTTATTATCCCG

Product: putative phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRPVVLCFSGLDPSGGAGLQADIEAIGQAGAHAAIACTAITIQNSQQVFGFEACTASLVNSQAVAVLDDLPVAVIKSGML
GTTDNIAMLSQLFADEIIVKGTPFVLDPVLVANSGGSLGDEQTLVSAFKQLLPYATLITPNTHELRALSGEQDLHIGAQK
LCAQGTHAVLVKTSHDFDSGDIEQYLYIKGEMVHKSILPRLKGEFHGSGCSLASFIAGRLAMGDALIEAVKAADSWITKT
LRAADAPHPEDAKAQLIPNRFMRF

Sequences:

>Translated_264_residues
MRPVVLCFSGLDPSGGAGLQADIEAIGQAGAHAAIACTAITIQNSQQVFGFEACTASLVNSQAVAVLDDLPVAVIKSGML
GTTDNIAMLSQLFADEIIVKGTPFVLDPVLVANSGGSLGDEQTLVSAFKQLLPYATLITPNTHELRALSGEQDLHIGAQK
LCAQGTHAVLVKTSHDFDSGDIEQYLYIKGEMVHKSILPRLKGEFHGSGCSLASFIAGRLAMGDALIEAVKAADSWITKT
LRAADAPHPEDAKAQLIPNRFMRF
>Mature_264_residues
MRPVVLCFSGLDPSGGAGLQADIEAIGQAGAHAAIACTAITIQNSQQVFGFEACTASLVNSQAVAVLDDLPVAVIKSGML
GTTDNIAMLSQLFADEIIVKGTPFVLDPVLVANSGGSLGDEQTLVSAFKQLLPYATLITPNTHELRALSGEQDLHIGAQK
LCAQGTHAVLVKTSHDFDSGDIEQYLYIKGEMVHKSILPRLKGEFHGSGCSLASFIAGRLAMGDALIEAVKAADSWITKT
LRAADAPHPEDAKAQLIPNRFMRF

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiaminase-2 family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=247, Percent_Identity=31.1740890688259, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325378, Length=253, Percent_Identity=32.0158102766798, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324517, Length=247, Percent_Identity=33.6032388663968, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324997, Length=247, Percent_Identity=33.6032388663968, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016084
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR004305
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF03070 TENA_THI-4; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27811; Mature: 27811

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVVLCFSGLDPSGGAGLQADIEAIGQAGAHAAIACTAITIQNSQQVFGFEACTASLVN
CCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHC
SQAVAVLDDLPVAVIKSGMLGTTDNIAMLSQLFADEIIVKGTPFVLDPVLVANSGGSLGD
CCCEEHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHEEECCCCCEECCEEEECCCCCCCC
EQTLVSAFKQLLPYATLITPNTHELRALSGEQDLHIGAQKLCAQGTHAVLVKTSHDFDSG
HHHHHHHHHHHCCEEEEECCCCHHHEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCC
DIEQYLYIKGEMVHKSILPRLKGEFHGSGCSLASFIAGRLAMGDALIEAVKAADSWITKT
CCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAADAPHPEDAKAQLIPNRFMRF
HHHCCCCCCCCCHHHHCCHHHHCC
>Mature Secondary Structure
MRPVVLCFSGLDPSGGAGLQADIEAIGQAGAHAAIACTAITIQNSQQVFGFEACTASLVN
CCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHC
SQAVAVLDDLPVAVIKSGMLGTTDNIAMLSQLFADEIIVKGTPFVLDPVLVANSGGSLGD
CCCEEHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHEEECCCCCEECCEEEECCCCCCCC
EQTLVSAFKQLLPYATLITPNTHELRALSGEQDLHIGAQKLCAQGTHAVLVKTSHDFDSG
HHHHHHHHHHHCCEEEEECCCCHHHEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCC
DIEQYLYIKGEMVHKSILPRLKGEFHGSGCSLASFIAGRLAMGDALIEAVKAADSWITKT
CCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAADAPHPEDAKAQLIPNRFMRF
HHHCCCCCCCCCHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]