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The map label for this gene is thiC [H]

Identifier: 93004924

GI number: 93004924

Start: 107114

End: 109000

Strand: Reverse

Name: thiC [H]

Synonym: Pcryo_0093

Alternate gene names: 93004924

Gene position: 109000-107114 (Counterclockwise)

Preceding gene: 93004925

Following gene: 93004921

Centisome position: 3.56

GC content: 48.44

Gene sequence:

>1887_bases
ATGACTATTTCAGATATTGGCTCGCAAGCCACCACACATACCCCTGTAAAAGCAAGCAAAGCAGACGCACTAAAAACTCC
CGCCCATCGCAGCGAAACTGACGCCCGTTTTGAAGAAGACGCACGCGACCTACATCGCATTTTGCCTGCCTCTCGCAAAG
TCTATATCGAAGGCTCACGCCCTGATATTCAAGTGCCGATGCGCGAGATTACCCTTGACCCTACTCCTATCCAAGGCGTG
TCCGAGTCCGAGTGGGAGCAAAATCCACCGTTTTATGTCTATGACACCTCAGGCGTCTATACCGACCCCAATGCCGCAAT
TGACTTGACCAAAGGTCTGCCAAAACTACGCGAAGGTTGGATAGATGAGCGCGGTGATACCGAACAGCTAGCTGGTTTAT
CAAGCTCATACGGTCTGGCGCGCGCACGTGATATCAGTACTGCAAACTTACGCTTTGCTCATATTGACAAGCCTCGCCGT
GCTAAAGCTGTTGATGGGAAAGTAGGTAACGTTACTCAATTGCACTATGCTCGCCGTGGCATTATTACCCCAGAAATGGA
ATATATAGCTATCCGCGAAACCCAAAAACAGCATGAGCTGACTGATATGCGCCAGCACGAAGGCGAGACTTTTGGTGCGC
ACACGCCAGCGATTATCACCCCAGAATTTGTCCGCAGTGAAGTTGCCGCGGGTCGCGCTATTATCCCTAACAATATCAAC
CATCCTGAATCTGAGCCGATGATCATCGGGCGTAATTTTTTGGTGAAAATTAATGCCAATATTGGTAACTCAGCACTAGG
TTCTTCTATTGATGAAGAAGTGTCGAAGATGACTTGGGCGACGCGTTGGGGTGCGGATAACATCATGGATCTGTCTACTG
GTAACCATATTCATGAAACCCGTGAATGGCTGATTCGTAACTCACCCGTACCGATTGGTACGGTGCCGATTTATCAAGCG
TTAGAAAAAGTCGATGGCGTAGCCGAAGATTTAACGTGGGAGATATTTCGCGATACTTTGATTGAGCAAGCCGAGCAAGG
TGTTGATTATTTCACCATTCACGCTGGCGTATTGCTTGAATACGTTCCGCTAACTGCCGGACGCTTAACCGGTATCGTCT
CGCGTGGTGGCTCTATCATGGCACAGTGGTGCATGTTTCATAATAAAGAAAGCTTTTTATATACCCATTTTGAAGACATC
TGCGAGATTATGAAACAGTATGATGTGGCATTTAGCTTAGGCGATGGCTTACGCCCAGGCTGCTTGCAAGATGCCAATGA
TGAAGCGCAGTTTGGTGAGCTGCGAACCCTTGGCGAGCTGACCCAAGTGGCATGGAAGCATGACGTACAGGTCATGATTG
AAGGTCCGGGACACGTGGCAATGAACCGTATTAAAGAAAATATGGATTTGCAGCTTGAAGTTTGTGCTGATGCGCCCTTT
TATACACTAGGACCCTTAACCACCGATATCGCACCGGGTTATGACCATATTACCAGTGCGATAGGCGCGGCGATGATTGG
CTGGTTTGGCACGGCGATGCTGTGTTATGTGACCCCAAAAGAGCATTTAGGGTTGCCGAATAAGAAAGATGTCAAAGATG
GCATCATCACTTATAAAATCGCCGCTCATGCTGCTGACCTTGCCAAAGGACACCCCGGTGCGCAGGCACGTGACAACGCG
TTATCCAAAGCGCGTTTTGAGTTCCGCTGGGACGATCAGTTCAATCTGGCGCTTGATCCGGATACTGCGCGTGAATTTCA
TGATGAAACCTTACCCAAAGATGCACACAAGACGGCACACTTTTGCTCCATGTGTGGTCCAAAGTTTTGTTCAATGAAAA
TCACTCAGAATGTCCGTGAGTATGCCAAAGGACTAAATGCCCAATAA

Upstream 100 bases:

>100_bases
ATTATTGTTTCGTGATTGAATTTATCGATATGGGATAGGCTTGCCCCGCAGATACTGGCGGTGTTGATTTTATTCAAAAA
CCAACACGCTAGGACAGCAT

Downstream 100 bases:

>100_bases
ACTGATAGAAAAATCTCACAAAGAAGCTCGGCAGTTATATAAAAAAGTATAGAAATACAACGCTAACAACTATTGTTCAA
GTATAAAAAAAGCCCCTGCA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MTISDIGSQATTHTPVKASKADALKTPAHRSETDARFEEDARDLHRILPASRKVYIEGSRPDIQVPMREITLDPTPIQGV
SESEWEQNPPFYVYDTSGVYTDPNAAIDLTKGLPKLREGWIDERGDTEQLAGLSSSYGLARARDISTANLRFAHIDKPRR
AKAVDGKVGNVTQLHYARRGIITPEMEYIAIRETQKQHELTDMRQHEGETFGAHTPAIITPEFVRSEVAAGRAIIPNNIN
HPESEPMIIGRNFLVKINANIGNSALGSSIDEEVSKMTWATRWGADNIMDLSTGNHIHETREWLIRNSPVPIGTVPIYQA
LEKVDGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLEYVPLTAGRLTGIVSRGGSIMAQWCMFHNKESFLYTHFEDI
CEIMKQYDVAFSLGDGLRPGCLQDANDEAQFGELRTLGELTQVAWKHDVQVMIEGPGHVAMNRIKENMDLQLEVCADAPF
YTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQARDNA
LSKARFEFRWDDQFNLALDPDTAREFHDETLPKDAHKTAHFCSMCGPKFCSMKITQNVREYAKGLNAQ

Sequences:

>Translated_628_residues
MTISDIGSQATTHTPVKASKADALKTPAHRSETDARFEEDARDLHRILPASRKVYIEGSRPDIQVPMREITLDPTPIQGV
SESEWEQNPPFYVYDTSGVYTDPNAAIDLTKGLPKLREGWIDERGDTEQLAGLSSSYGLARARDISTANLRFAHIDKPRR
AKAVDGKVGNVTQLHYARRGIITPEMEYIAIRETQKQHELTDMRQHEGETFGAHTPAIITPEFVRSEVAAGRAIIPNNIN
HPESEPMIIGRNFLVKINANIGNSALGSSIDEEVSKMTWATRWGADNIMDLSTGNHIHETREWLIRNSPVPIGTVPIYQA
LEKVDGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLEYVPLTAGRLTGIVSRGGSIMAQWCMFHNKESFLYTHFEDI
CEIMKQYDVAFSLGDGLRPGCLQDANDEAQFGELRTLGELTQVAWKHDVQVMIEGPGHVAMNRIKENMDLQLEVCADAPF
YTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQARDNA
LSKARFEFRWDDQFNLALDPDTAREFHDETLPKDAHKTAHFCSMCGPKFCSMKITQNVREYAKGLNAQ
>Mature_627_residues
TISDIGSQATTHTPVKASKADALKTPAHRSETDARFEEDARDLHRILPASRKVYIEGSRPDIQVPMREITLDPTPIQGVS
ESEWEQNPPFYVYDTSGVYTDPNAAIDLTKGLPKLREGWIDERGDTEQLAGLSSSYGLARARDISTANLRFAHIDKPRRA
KAVDGKVGNVTQLHYARRGIITPEMEYIAIRETQKQHELTDMRQHEGETFGAHTPAIITPEFVRSEVAAGRAIIPNNINH
PESEPMIIGRNFLVKINANIGNSALGSSIDEEVSKMTWATRWGADNIMDLSTGNHIHETREWLIRNSPVPIGTVPIYQAL
EKVDGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLEYVPLTAGRLTGIVSRGGSIMAQWCMFHNKESFLYTHFEDIC
EIMKQYDVAFSLGDGLRPGCLQDANDEAQFGELRTLGELTQVAWKHDVQVMIEGPGHVAMNRIKENMDLQLEVCADAPFY
TLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQARDNAL
SKARFEFRWDDQFNLALDPDTAREFHDETLPKDAHKTAHFCSMCGPKFCSMKITQNVREYAKGLNAQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=598, Percent_Identity=70.5685618729097, Blast_Score=873, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 69880; Mature: 69748

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISDIGSQATTHTPVKASKADALKTPAHRSETDARFEEDARDLHRILPASRKVYIEGSR
CCCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
PDIQVPMREITLDPTPIQGVSESEWEQNPPFYVYDTSGVYTDPNAAIDLTKGLPKLREGW
CCCCCCHHHEECCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCEEHHCCCHHHHHCC
IDERGDTEQLAGLSSSYGLARARDISTANLRFAHIDKPRRAKAVDGKVGNVTQLHYARRG
CCCCCCHHHHHCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCC
IITPEMEYIAIRETQKQHELTDMRQHEGETFGAHTPAIITPEFVRSEVAAGRAIIPNNIN
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHCCCEECCCCCC
HPESEPMIIGRNFLVKINANIGNSALGSSIDEEVSKMTWATRWGADNIMDLSTGNHIHET
CCCCCCEEECCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
REWLIRNSPVPIGTVPIYQALEKVDGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLE
HHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
YVPLTAGRLTGIVSRGGSIMAQWCMFHNKESFLYTHFEDICEIMKQYDVAFSLGDGLRPG
HHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCEEECCCCCCCC
CLQDANDEAQFGELRTLGELTQVAWKHDVQVMIEGPGHVAMNRIKENMDLQLEVCADAPF
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCC
YTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKKDVKDGIITYKI
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCEEEEEH
AAHAADLAKGHPGAQARDNALSKARFEFRWDDQFNLALDPDTAREFHDETLPKDAHKTAH
HHHHHHHHCCCCCCCHHHHHHHHCEEEEEECCCEEEEECCHHHHHHHHHCCCCHHHHHHH
FCSMCGPKFCSMKITQNVREYAKGLNAQ
HHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TISDIGSQATTHTPVKASKADALKTPAHRSETDARFEEDARDLHRILPASRKVYIEGSR
CCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
PDIQVPMREITLDPTPIQGVSESEWEQNPPFYVYDTSGVYTDPNAAIDLTKGLPKLREGW
CCCCCCHHHEECCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCEEHHCCCHHHHHCC
IDERGDTEQLAGLSSSYGLARARDISTANLRFAHIDKPRRAKAVDGKVGNVTQLHYARRG
CCCCCCHHHHHCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCC
IITPEMEYIAIRETQKQHELTDMRQHEGETFGAHTPAIITPEFVRSEVAAGRAIIPNNIN
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHCCCEECCCCCC
HPESEPMIIGRNFLVKINANIGNSALGSSIDEEVSKMTWATRWGADNIMDLSTGNHIHET
CCCCCCEEECCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
REWLIRNSPVPIGTVPIYQALEKVDGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLE
HHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
YVPLTAGRLTGIVSRGGSIMAQWCMFHNKESFLYTHFEDICEIMKQYDVAFSLGDGLRPG
HHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCEEECCCCCCCC
CLQDANDEAQFGELRTLGELTQVAWKHDVQVMIEGPGHVAMNRIKENMDLQLEVCADAPF
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCC
YTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKKDVKDGIITYKI
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCEEEEEH
AAHAADLAKGHPGAQARDNALSKARFEFRWDDQFNLALDPDTAREFHDETLPKDAHKTAH
HHHHHHHHCCCCCCCHHHHHHHHCEEEEEECCCEEEEECCHHHHHHHHHCCCCHHHHHHH
FCSMCGPKFCSMKITQNVREYAKGLNAQ
HHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA