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The map label for this gene is def [H]

Identifier: 93004869

GI number: 93004869

Start: 45620

End: 46174

Strand: Reverse

Name: def [H]

Synonym: Pcryo_0038

Alternate gene names: 93004869

Gene position: 46174-45620 (Counterclockwise)

Preceding gene: 93004870

Following gene: 93004868

Centisome position: 1.51

GC content: 45.41

Gene sequence:

>555_bases
ATGGCGTTACTCCCTATTTTAAGCTATCCCGACCCGCGTCTGCGTACGATTGCAACGCCTGTCAAGGAAGTCACTGCTGA
AATCAAAACCTTAATCACCGATATGATTGAGACCATGTACGATGCGCAAGGTATCGGTCTGGCTGCAAGCCAAGTGGATC
ACCATATCCAGCTTATCGTGATGGATTTATCAGAAGATAAAGACAGCCCAAGAGTGTTTATCAATCCCAAAGTAACGCCA
TTGGTTGAAGAAAAGCAGCCGTATGAAGAAGGTTGTCTGTCCGTGCCAGACGTCTATGACAAAGTTGAGCGCCCAAACAA
AGTGCGTATCGAAGCACTAGATGAGAATGGCAATAAGATTGATGAAGAAGTAGAAGGCTTACTTGCCGTTTGCATCCAAC
ACGAAATGGATCATCTGAATGGGGTGATTTTCGTCGATTATTTGTCGCGTTTGAAACAGACACGTGCGCGTGATAAGGTG
CGTAAAGTCCTTAAAATACGTGAAAAACAAGGCGAGCAAGTGGCGGAAAAAGAGCCACAGCCAGCAAACTCTTAA

Upstream 100 bases:

>100_bases
TCAGGATATCTCGCTTTAGCTATTGATAAGCATTACTTTTGTCCCCATTTATCTTATAATATCAAACCTATTTTTTATCT
TTATCATTGAGGTGAGCCTT

Downstream 100 bases:

>100_bases
TCTATTTCTCTGATAAGTTGATTTCACATACGTTTATCGTCAAGTGGTTTTGTTATGGCTTATGCTACGTTTTAAACGTT
GTCTGTTTTAGATACTGCAC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MALLPILSYPDPRLRTIATPVKEVTAEIKTLITDMIETMYDAQGIGLAASQVDHHIQLIVMDLSEDKDSPRVFINPKVTP
LVEEKQPYEEGCLSVPDVYDKVERPNKVRIEALDENGNKIDEEVEGLLAVCIQHEMDHLNGVIFVDYLSRLKQTRARDKV
RKVLKIREKQGEQVAEKEPQPANS

Sequences:

>Translated_184_residues
MALLPILSYPDPRLRTIATPVKEVTAEIKTLITDMIETMYDAQGIGLAASQVDHHIQLIVMDLSEDKDSPRVFINPKVTP
LVEEKQPYEEGCLSVPDVYDKVERPNKVRIEALDENGNKIDEEVEGLLAVCIQHEMDHLNGVIFVDYLSRLKQTRARDKV
RKVLKIREKQGEQVAEKEPQPANS
>Mature_183_residues
ALLPILSYPDPRLRTIATPVKEVTAEIKTLITDMIETMYDAQGIGLAASQVDHHIQLIVMDLSEDKDSPRVFINPKVTPL
VEEKQPYEEGCLSVPDVYDKVERPNKVRIEALDENGNKIDEEVEGLLAVCIQHEMDHLNGVIFVDYLSRLKQTRARDKVR
KVLKIREKQGEQVAEKEPQPANS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=155, Percent_Identity=32.258064516129, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=51.4792899408284, Blast_Score=169, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=33.1210191082803, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=32.7044025157233, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20855; Mature: 20724

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPILSYPDPRLRTIATPVKEVTAEIKTLITDMIETMYDAQGIGLAASQVDHHIQLIV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEE
MDLSEDKDSPRVFINPKVTPLVEEKQPYEEGCLSVPDVYDKVERPNKVRIEALDENGNKI
EECCCCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCH
DEEVEGLLAVCIQHEMDHLNGVIFVDYLSRLKQTRARDKVRKVLKIREKQGEQVAEKEPQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
PANS
CCCC
>Mature Secondary Structure 
ALLPILSYPDPRLRTIATPVKEVTAEIKTLITDMIETMYDAQGIGLAASQVDHHIQLIV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEE
MDLSEDKDSPRVFINPKVTPLVEEKQPYEEGCLSVPDVYDKVERPNKVRIEALDENGNKI
EECCCCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCH
DEEVEGLLAVCIQHEMDHLNGVIFVDYLSRLKQTRARDKVRKVLKIREKQGEQVAEKEPQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
PANS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA