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The map label for this gene is ligT [C]

Identifier: 92115355

GI number: 92115355

Start: 3612640

End: 3613164

Strand: Direct

Name: ligT [C]

Synonym: Csal_3240

Alternate gene names: 92115355

Gene position: 3612640-3613164 (Clockwise)

Preceding gene: 92115354

Following gene: 92115356

Centisome position: 97.73

GC content: 70.48

Gene sequence:

>525_bases
GTGACCGTTTCTCGCCGACTCTTCTTCGCCCTGTGGCCCGACGACACCACTCGCCGGGCGCTGGCCGCCGAGGCTCAGCG
CCTGGCGCCACTCTGCGGCGGCTACCCGCTGCCCGCGGAGAACATGCATATCACGCTGGCGTTTCTGGGCAATGTCGACG
AGGCCCGAATCGCCCCACTCGTCGCCCTCGCGCAGGCCTGGCCGGCGCTGCAGGGCGAGTGGACGCTGGATCGGCTGGGG
CATTTCCCCAAGCCCCGCATCGTCTGGGCGGGCAGCCAGGCGTCGAGTCCCGAGCTGATGGCGCTCGATGCCGAGCTCTG
GCAGGCGCTGGCCCGTCATGGCTTCACCGCGCCGCAGCGTGACTTCACCCCGCATGTCAGCCTGGTGCGCCAGGCCGACC
GCGCGGCACCGGCGTCACGGCTGCCCACCCCGATCCGCTGGCGCTTCGACCGTCTCGCCCTGGTGGAATCGCAGCTCGAC
AATGGTGGCTCGCGCTATCGGACGTTGGCGTGCGGTGTCGATTGA

Upstream 100 bases:

>100_bases
CTTCTTGTGGGGACAGGCCTCGTGCGGACCGTGCCTGACACCGTTGGCAAGAAAGGGCTGATGCGTCACCCTGCTGGCAA
CCCCACCGGGAGCCGCCATC

Downstream 100 bases:

>100_bases
TACCACCAGCATGAATTGCCCAGAAGAATGACGGTACGCGTTGCCAAATCTGCCAGGAGAAAGTGCTGAACATCCGCCAG
GATGCTGTTCAAAGCGCGTA

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: 2 5' RNA Ligase; 2\-5\' RNA Ligase; 2-5 RNA Ligase

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTVSRRLFFALWPDDTTRRALAAEAQRLAPLCGGYPLPAENMHITLAFLGNVDEARIAPLVALAQAWPALQGEWTLDRLG
HFPKPRIVWAGSQASSPELMALDAELWQALARHGFTAPQRDFTPHVSLVRQADRAAPASRLPTPIRWRFDRLALVESQLD
NGGSRYRTLACGVD

Sequences:

>Translated_174_residues
MTVSRRLFFALWPDDTTRRALAAEAQRLAPLCGGYPLPAENMHITLAFLGNVDEARIAPLVALAQAWPALQGEWTLDRLG
HFPKPRIVWAGSQASSPELMALDAELWQALARHGFTAPQRDFTPHVSLVRQADRAAPASRLPTPIRWRFDRLALVESQLD
NGGSRYRTLACGVD
>Mature_173_residues
TVSRRLFFALWPDDTTRRALAAEAQRLAPLCGGYPLPAENMHITLAFLGNVDEARIAPLVALAQAWPALQGEWTLDRLGH
FPKPRIVWAGSQASSPELMALDAELWQALARHGFTAPQRDFTPHVSLVRQADRAAPASRLPTPIRWRFDRLALVESQLDN
GGSRYRTLACGVD

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=173, Percent_Identity=31.7919075144509, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19306; Mature: 19175

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSRRLFFALWPDDTTRRALAAEAQRLAPLCGGYPLPAENMHITLAFLGNVDEARIAPL
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHH
VALAQAWPALQGEWTLDRLGHFPKPRIVWAGSQASSPELMALDAELWQALARHGFTAPQR
HHHHHHCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCEEEECHHHHHHHHHCCCCCCCC
DFTPHVSLVRQADRAAPASRLPTPIRWRFDRLALVESQLDNGGSRYRTLACGVD
CCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure 
TVSRRLFFALWPDDTTRRALAAEAQRLAPLCGGYPLPAENMHITLAFLGNVDEARIAPL
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHH
VALAQAWPALQGEWTLDRLGHFPKPRIVWAGSQASSPELMALDAELWQALARHGFTAPQR
HHHHHHCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCEEEECHHHHHHHHHCCCCCCCC
DFTPHVSLVRQADRAAPASRLPTPIRWRFDRLALVESQLDNGGSRYRTLACGVD
CCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA