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The map label for this gene is exoA [H]

Identifier: 92115320

GI number: 92115320

Start: 3572402

End: 3573169

Strand: Direct

Name: exoA [H]

Synonym: Csal_3205

Alternate gene names: 92115320

Gene position: 3572402-3573169 (Clockwise)

Preceding gene: 92115313

Following gene: 92115322

Centisome position: 96.64

GC content: 60.68

Gene sequence:

>768_bases
ATGAAAATCGCCAGCATCAATGTCAACGGGATCCGCGAAGCGGTCGGGAGGGGGTTTCTCGACTGGCTGGCCAACCAGGA
CGCCGACGTCGTCTGCGTGCAGAATCTCAAGGCCAAAAGCTTCGAACTCGACGACAGCATCCTCTTTCCCGAAGGGTACG
AAGGTTACTTCCTGGATGCCGAGGAAGACGGCTTCTCCGGCGTGGGGCTCTATTGCCGCACGATTCCCAAGGCCATCATG
TACGGGCTGGGTTTCCCCCAGTGCGATAACGAAGCGCGTTTCCTGCAGGCCGATTACGAGCGCTTCAGCATCGCCAGCTT
CCTGATGCCCGACGGCAGCGATCTGGAGGCCAAGCAGGCCTTCATGAATCAGTACCAGGAATATCTCAGCAAGATGGTGC
GCAAGCGCCGCGAGTACATCATCTGCGGAACCTGGCACATCGCGCACAAGACCATCGACCTCGCCAACTGGGCCGACAAC
CAGACCACGCCGGGCTTTCGCCCCGAAGAGCGTGCCTGGATGGATCAGGTCTTCGGTCCGATGGGCTACATCGACACCTT
CCGTGAGGTCAATCGCGACGCCAATCAGTACACCTGGTGGCCCAAGCTCGACCAGGACGTGCCGCGCGCGCGACAGGAAG
GCTGGCGTCTGGACTACCAGATCGTCGGTCCCGAGTTCCGCCGCCACGTGGTGGATGCCTGGATCGACCGCGACGCGACC
TTCTCCGAATTCGCGCCGCTCATCGTCGAGTACGATCTCGAACTGTAA

Upstream 100 bases:

>100_bases
GCAGTCACGCTGACTCCCTATCACCCTGATCGTTTCTACACATTTACCTAAACGTCGAAAGCTCAGGTATCATACACGCG
CGACGAAAAAGGGACCATGT

Downstream 100 bases:

>100_bases
CGTCGCCGGTCCCCGCGCCCATGACGACGCCGGCCTCTGGCCGGCGTCGTGCGTTTTCAGTCACGAAAACCCAGTGCCTC
GCGCTGATGCGCGAACAGCT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKIASINVNGIREAVGRGFLDWLANQDADVVCVQNLKAKSFELDDSILFPEGYEGYFLDAEEDGFSGVGLYCRTIPKAIM
YGLGFPQCDNEARFLQADYERFSIASFLMPDGSDLEAKQAFMNQYQEYLSKMVRKRREYIICGTWHIAHKTIDLANWADN
QTTPGFRPEERAWMDQVFGPMGYIDTFREVNRDANQYTWWPKLDQDVPRARQEGWRLDYQIVGPEFRRHVVDAWIDRDAT
FSEFAPLIVEYDLEL

Sequences:

>Translated_255_residues
MKIASINVNGIREAVGRGFLDWLANQDADVVCVQNLKAKSFELDDSILFPEGYEGYFLDAEEDGFSGVGLYCRTIPKAIM
YGLGFPQCDNEARFLQADYERFSIASFLMPDGSDLEAKQAFMNQYQEYLSKMVRKRREYIICGTWHIAHKTIDLANWADN
QTTPGFRPEERAWMDQVFGPMGYIDTFREVNRDANQYTWWPKLDQDVPRARQEGWRLDYQIVGPEFRRHVVDAWIDRDAT
FSEFAPLIVEYDLEL
>Mature_255_residues
MKIASINVNGIREAVGRGFLDWLANQDADVVCVQNLKAKSFELDDSILFPEGYEGYFLDAEEDGFSGVGLYCRTIPKAIM
YGLGFPQCDNEARFLQADYERFSIASFLMPDGSDLEAKQAFMNQYQEYLSKMVRKRREYIICGTWHIAHKTIDLANWADN
QTTPGFRPEERAWMDQVFGPMGYIDTFREVNRDANQYTWWPKLDQDVPRARQEGWRLDYQIVGPEFRRHVVDAWIDRDAT
FSEFAPLIVEYDLEL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=28.515625, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=28.515625, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=28.515625, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1788046, Length=257, Percent_Identity=25.6809338521401, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71989536, Length=253, Percent_Identity=26.8774703557312, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=30.2325581395349, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=30.2325581395349, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29681; Mature: 29681

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIASINVNGIREAVGRGFLDWLANQDADVVCVQNLKAKSFELDDSILFPEGYEGYFLDA
CEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEECCCCEECCCCCCCEEECC
EEDGFSGVGLYCRTIPKAIMYGLGFPQCDNEARFLQADYERFSIASFLMPDGSDLEAKQA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FMNQYQEYLSKMVRKRREYIICGTWHIAHKTIDLANWADNQTTPGFRPEERAWMDQVFGP
HHHHHHHHHHHHHHHHCCEEEEEEEEHHHHEEEHHCCCCCCCCCCCCCHHHHHHHHHHCC
MGYIDTFREVNRDANQYTWWPKLDQDVPRARQEGWRLDYQIVGPEFRRHVVDAWIDRDAT
HHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCC
FSEFAPLIVEYDLEL
HHHHCCEEEEEECCC
>Mature Secondary Structure
MKIASINVNGIREAVGRGFLDWLANQDADVVCVQNLKAKSFELDDSILFPEGYEGYFLDA
CEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEECCCCEECCCCCCCEEECC
EEDGFSGVGLYCRTIPKAIMYGLGFPQCDNEARFLQADYERFSIASFLMPDGSDLEAKQA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FMNQYQEYLSKMVRKRREYIICGTWHIAHKTIDLANWADNQTTPGFRPEERAWMDQVFGP
HHHHHHHHHHHHHHHHCCEEEEEEEEHHHHEEEHHCCCCCCCCCCCCCHHHHHHHHHHCC
MGYIDTFREVNRDANQYTWWPKLDQDVPRARQEGWRLDYQIVGPEFRRHVVDAWIDRDAT
HHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCC
FSEFAPLIVEYDLEL
HHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]