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The map label for this gene is rhmD [H]

Identifier: 92115310

GI number: 92115310

Start: 3562167

End: 3563339

Strand: Direct

Name: rhmD [H]

Synonym: Csal_3195

Alternate gene names: 92115310

Gene position: 3562167-3563339 (Clockwise)

Preceding gene: 92115309

Following gene: 92115311

Centisome position: 96.36

GC content: 61.04

Gene sequence:

>1173_bases
ATGAAAATCACGCGCGTAAGAGCCCGCGTCTTCGAATGGAAAGGCGACACAGTGCCACCGCAGCCGCATTTCTGCACCAA
TGCCATGGATGCCCTGTGGGATCGGGGCGATGCCATGGGCAGTTTCCGGTTTCATGGCTGGACGGTGGTCGAGGTCGAAA
CGGACGAAGGGATCGTCGGGCTCGGCAACGTGGCCCTGGCGCCACGTGTCGCCAAGACGATCATCGATCAGCATCTTGCT
CCTCTGGTCATCGGCCAGGACCCGTTCGACTACGAGTACCTGTGGCAGCGCATGTATCGCGCAACGCTGGCGTGGGGACG
GCGCGGTATCGGCATGGCGGCGATCTCGGGCGTCGACATCGCCATCTGGGACATCATGGGCAAGGCGCTTGGCAAGCCGG
TATTCAAGCTGCTGGGCGGGCGGACCAAGGAGAAGATCCCGTGCTACGCCTCCAAACTCTACAACACCGATCGCGAGACG
ATGCAGCACGAGGCGCAAGCCTATCTCGACCAGGGCTTCACGGCGATGAAGATGCGTTTCGGCTATGGGCCCAAGGATGG
GCCCCGCGGCATGCGCGAGAACCTCAAGAGCGTGGCCGCGGTACGCGAGGTGATCGGCGAGGACATCGACCTGATGGCCG
ATTGCTACATGGGATGGAATCTCGACTATGCCCGGCGCATGCTGCCCCGCCTCGAACCCTTCGAGCTGCGTTGGCTGGAG
GAGCCGGTGATCGCCGATGACATCGACGGCTACGCCGAACTCAACCGCATCGCCCCCATGCCGATTTCCGGCGGCGAGCA
CGAATTCACCGTGCACGGTTTCCGACAACTCCTGGAGAAGAAAGCCGTCTCGGTGATTCAGTACGATACCAACCGCGTCG
GCGGCATCAGCGTGGCGCGCAAGATCAACGCCATGGCCGAGGCTTTCGAGGTACCGGTGATCCCGCATGCGGGGCAGATG
CACAACTACCATCTGACGATGGCCTCGCTGGCCTCGCCGATGTCCGAATTCTTCCCGGTGCATGACGTCGAGATCGGCAA
TGAGCTTTTCTACTACCTGTTCGAGGGCGATCCAGAGCCCATCGACGGATATCTCGACCTAAACGACGACCTTCCAGGAC
TTGGGCTCACACTCAGTGAAACCAATTTTGACCAGTTCCACATCGTCGAGTAG

Upstream 100 bases:

>100_bases
CTGCGCCCCACTCACAATGCTTTCAAATTGGCTTGCCAAATGGCGGCCAGCAACCGGCTTTCCAGCTAACTCCTCATCAC
GACGCTAGACGGACAAGATT

Downstream 100 bases:

>100_bases
GCAATTCTCGACGGCAATATTCATTCTTTGATCAGTAGAGGAGGCAGTCAACGCTTTACGCTCACAAGAACAATCGCTCA
GCTCACAGACATATCGGGAG

Product: mandelate racemase/muconate lactonizing enzyme

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKITRVRARVFEWKGDTVPPQPHFCTNAMDALWDRGDAMGSFRFHGWTVVEVETDEGIVGLGNVALAPRVAKTIIDQHLA
PLVIGQDPFDYEYLWQRMYRATLAWGRRGIGMAAISGVDIAIWDIMGKALGKPVFKLLGGRTKEKIPCYASKLYNTDRET
MQHEAQAYLDQGFTAMKMRFGYGPKDGPRGMRENLKSVAAVREVIGEDIDLMADCYMGWNLDYARRMLPRLEPFELRWLE
EPVIADDIDGYAELNRIAPMPISGGEHEFTVHGFRQLLEKKAVSVIQYDTNRVGGISVARKINAMAEAFEVPVIPHAGQM
HNYHLTMASLASPMSEFFPVHDVEIGNELFYYLFEGDPEPIDGYLDLNDDLPGLGLTLSETNFDQFHIVE

Sequences:

>Translated_390_residues
MKITRVRARVFEWKGDTVPPQPHFCTNAMDALWDRGDAMGSFRFHGWTVVEVETDEGIVGLGNVALAPRVAKTIIDQHLA
PLVIGQDPFDYEYLWQRMYRATLAWGRRGIGMAAISGVDIAIWDIMGKALGKPVFKLLGGRTKEKIPCYASKLYNTDRET
MQHEAQAYLDQGFTAMKMRFGYGPKDGPRGMRENLKSVAAVREVIGEDIDLMADCYMGWNLDYARRMLPRLEPFELRWLE
EPVIADDIDGYAELNRIAPMPISGGEHEFTVHGFRQLLEKKAVSVIQYDTNRVGGISVARKINAMAEAFEVPVIPHAGQM
HNYHLTMASLASPMSEFFPVHDVEIGNELFYYLFEGDPEPIDGYLDLNDDLPGLGLTLSETNFDQFHIVE
>Mature_390_residues
MKITRVRARVFEWKGDTVPPQPHFCTNAMDALWDRGDAMGSFRFHGWTVVEVETDEGIVGLGNVALAPRVAKTIIDQHLA
PLVIGQDPFDYEYLWQRMYRATLAWGRRGIGMAAISGVDIAIWDIMGKALGKPVFKLLGGRTKEKIPCYASKLYNTDRET
MQHEAQAYLDQGFTAMKMRFGYGPKDGPRGMRENLKSVAAVREVIGEDIDLMADCYMGWNLDYARRMLPRLEPFELRWLE
EPVIADDIDGYAELNRIAPMPISGGEHEFTVHGFRQLLEKKAVSVIQYDTNRVGGISVARKINAMAEAFEVPVIPHAGQM
HNYHLTMASLASPMSEFFPVHDVEIGNELFYYLFEGDPEPIDGYLDLNDDLPGLGLTLSETNFDQFHIVE

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=329, Percent_Identity=26.4437689969605, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI186972148, Length=272, Percent_Identity=27.9411764705882, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI42544117, Length=326, Percent_Identity=25.7668711656442, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI226510960, Length=339, Percent_Identity=33.3333333333333, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI48994953, Length=349, Percent_Identity=30.945558739255, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787864, Length=301, Percent_Identity=26.2458471760797, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 44041; Mature: 44041

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRVRARVFEWKGDTVPPQPHFCTNAMDALWDRGDAMGSFRFHGWTVVEVETDEGIVG
CCCHHEEHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCEEEEEEECCCCEEE
LGNVALAPRVAKTIIDQHLAPLVIGQDPFDYEYLWQRMYRATLAWGRRGIGMAAISGVDI
CCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCHH
AIWDIMGKALGKPVFKLLGGRTKEKIPCYASKLYNTDRETMQHEAQAYLDQGFTAMKMRF
HHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEE
GYGPKDGPRGMRENLKSVAAVREVIGEDIDLMADCYMGWNLDYARRMLPRLEPFELRWLE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHEECC
EPVIADDIDGYAELNRIAPMPISGGEHEFTVHGFRQLLEKKAVSVIQYDTNRVGGISVAR
CCCCCCCCCCHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHEEEEECCCCCCHHHHHH
KINAMAEAFEVPVIPHAGQMHNYHLTMASLASPMSEFFPVHDVEIGNELFYYLFEGDPEP
HHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEECCEEEEEEECCCCCC
IDGYLDLNDDLPGLGLTLSETNFDQFHIVE
CCCEEECCCCCCCCEEEEECCCCCEEEECC
>Mature Secondary Structure
MKITRVRARVFEWKGDTVPPQPHFCTNAMDALWDRGDAMGSFRFHGWTVVEVETDEGIVG
CCCHHEEHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCEEEEEEECCCCEEE
LGNVALAPRVAKTIIDQHLAPLVIGQDPFDYEYLWQRMYRATLAWGRRGIGMAAISGVDI
CCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCHH
AIWDIMGKALGKPVFKLLGGRTKEKIPCYASKLYNTDRETMQHEAQAYLDQGFTAMKMRF
HHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEE
GYGPKDGPRGMRENLKSVAAVREVIGEDIDLMADCYMGWNLDYARRMLPRLEPFELRWLE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHEECC
EPVIADDIDGYAELNRIAPMPISGGEHEFTVHGFRQLLEKKAVSVIQYDTNRVGGISVAR
CCCCCCCCCCHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHEEEEECCCCCCHHHHHH
KINAMAEAFEVPVIPHAGQMHNYHLTMASLASPMSEFFPVHDVEIGNELFYYLFEGDPEP
HHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEECCEEEEEEECCCCCC
IDGYLDLNDDLPGLGLTLSETNFDQFHIVE
CCCEEECCCCCCCCEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA