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The map label for this gene is dsbA [H]

Identifier: 92115233

GI number: 92115233

Start: 3472463

End: 3473095

Strand: Direct

Name: dsbA [H]

Synonym: Csal_3118

Alternate gene names: 92115233

Gene position: 3472463-3473095 (Clockwise)

Preceding gene: 92115231

Following gene: 92115234

Centisome position: 93.94

GC content: 62.56

Gene sequence:

>633_bases
ATGTTGAAGTCGTTGTTAGTGCTGGTCACCGGCATGAGCCTGTCAACGCTGGTCATGGCCGCCGAGCCGGTCGCCGGGGA
GGATTACACGGTGCTCGACGAGCCGGTGAAGACCGAGGTACCGGAGGGCAAGATCGAGGTCAACGAGGTGTTCTGGTACG
GCTGTCCGCATTGCTACGCGCTCGAAGCGCCGCTCAATGCCTGGGTCGATGAACTGCCCGACGATGTCGCCTTCCAGCGC
ATTCCCGCCACCATGGGCGAGACGTGGACCAAGCATGCCCGTGCCTTCTACGCCGCCAAGGAACTGGGCATTCAGGAAGA
CATGCATAGCGACTTCTTCGATGCCATTCACGAACAGGGGCAACGCCTCACCGAACCGGACGACATCGCCGAATTCTTCA
CCCAGTACGGGGTCAGCAAGGACGAGGCGCTCGAGGCCCTCGACTCGTTCGGCGTCAAAAGCCAGCTCAACCAGGCCAGC
GCCAAGATGCGCGGCTACCAGATCATGGGCGTGCCTGCCCTGGTCGTCGACGGGCGCTACGTGATCACGCCCAGCAGCGC
CGGCGCGCTGGACAACATGCCCAAGATCGCCGACGCCCTGATCGACAAGGTACGCAGCGAACGCGATGAGTAA

Upstream 100 bases:

>100_bases
CCGACGCGGCTCGAACATTGAGCGAAGGTGGCGATCGCGTGCCGTCCGCGTTCCACCCGGCCCGTTCGCTCAAGGCCCGT
TTTTCATCAAGGAGAGTTGC

Downstream 100 bases:

>100_bases
TGCCGCCGCGGCGCCCGCCTCCCGGGCGCCTCACTCGTTACGCCTGCTGACCTGCAACTTGCAGGTCGGCATCCATACGT
CGGCCTATCATCATTATCTC

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MLKSLLVLVTGMSLSTLVMAAEPVAGEDYTVLDEPVKTEVPEGKIEVNEVFWYGCPHCYALEAPLNAWVDELPDDVAFQR
IPATMGETWTKHARAFYAAKELGIQEDMHSDFFDAIHEQGQRLTEPDDIAEFFTQYGVSKDEALEALDSFGVKSQLNQAS
AKMRGYQIMGVPALVVDGRYVITPSSAGALDNMPKIADALIDKVRSERDE

Sequences:

>Translated_210_residues
MLKSLLVLVTGMSLSTLVMAAEPVAGEDYTVLDEPVKTEVPEGKIEVNEVFWYGCPHCYALEAPLNAWVDELPDDVAFQR
IPATMGETWTKHARAFYAAKELGIQEDMHSDFFDAIHEQGQRLTEPDDIAEFFTQYGVSKDEALEALDSFGVKSQLNQAS
AKMRGYQIMGVPALVVDGRYVITPSSAGALDNMPKIADALIDKVRSERDE
>Mature_210_residues
MLKSLLVLVTGMSLSTLVMAAEPVAGEDYTVLDEPVKTEVPEGKIEVNEVFWYGCPHCYALEAPLNAWVDELPDDVAFQR
IPATMGETWTKHARAFYAAKELGIQEDMHSDFFDAIHEQGQRLTEPDDIAEFFTQYGVSKDEALEALDSFGVKSQLNQAS
AKMRGYQIMGVPALVVDGRYVITPSSAGALDNMPKIADALIDKVRSERDE

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=188, Percent_Identity=28.1914893617021, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23220; Mature: 23220

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSLLVLVTGMSLSTLVMAAEPVAGEDYTVLDEPVKTEVPEGKIEVNEVFWYGCPHCYA
CHHHHHHHHHCCCHHHHHHHCCCCCCCCCEECCCCHHCCCCCCCEEEEEEEEECCCCCEE
LEAPLNAWVDELPDDVAFQRIPATMGETWTKHARAFYAAKELGIQEDMHSDFFDAIHEQG
ECCCHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
QRLTEPDDIAEFFTQYGVSKDEALEALDSFGVKSQLNQASAKMRGYQIMGVPALVVDGRY
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCEEEECCCEEEECCEE
VITPSSAGALDNMPKIADALIDKVRSERDE
EECCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKSLLVLVTGMSLSTLVMAAEPVAGEDYTVLDEPVKTEVPEGKIEVNEVFWYGCPHCYA
CHHHHHHHHHCCCHHHHHHHCCCCCCCCCEECCCCHHCCCCCCCEEEEEEEEECCCCCEE
LEAPLNAWVDELPDDVAFQRIPATMGETWTKHARAFYAAKELGIQEDMHSDFFDAIHEQG
ECCCHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
QRLTEPDDIAEFFTQYGVSKDEALEALDSFGVKSQLNQASAKMRGYQIMGVPALVVDGRY
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCEEEECCCEEEECCEE
VITPSSAGALDNMPKIADALIDKVRSERDE
EECCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9099867 [H]