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The map label for this gene is dapF

Identifier: 92115228

GI number: 92115228

Start: 3468241

End: 3469074

Strand: Direct

Name: dapF

Synonym: Csal_3113

Alternate gene names: 92115228

Gene position: 3468241-3469074 (Clockwise)

Preceding gene: 92115227

Following gene: 92115229

Centisome position: 93.82

GC content: 68.23

Gene sequence:

>834_bases
ATGCTGCTGCACTTCACCAAGATGCACGGGCTCGGCAACGACTTCGTGGTGATCGACCTGATCACCCAGCGGGCACGGCT
CGACACCGAGCGCATTCGGCAACTCGCCGATCGCCATGTCGGCATCGGGTTCGACCAACTGCTGCTCGTCGAGCCGCCGC
GCGATCCGGAGATGGATTTTCGCTATCGCATCTTCAACGCCGACGGCAACGAAGTGGAGAACTGCGGCAATGGCGCCCGC
TGCTTCGCGCGCTTTGTGCGCGATAATCGCCTGACCCACAAGCGCGAGATTCGTGTCGAGACCCAGGCCGGCCCCCTGAC
CCTGCGCGTCGAGGATGACGAACGAATCCGCGTGGACATGGGCGTGCCACGCTTTGCTCCCGAGGTCGTGCCCTTCGATG
CCCCGGCCGATGCCCCGGCGCACTGCCTGGAGGTCGATGGCGAGACCTGGGACATCGGCGCGGTCTCGATGGGCAACCCG
CACGCCGTATTGCGTGTCGACGACGTCGCGCAAGCACCGGTGGCGCGGCTCGGCCCGCTCATCGAACGCCATCCGCGCTT
CCCTCGGGGCGTCAACGTCGGCTTCCTGCAGGTCGTCTCGCGTCACGAGGCTCGCCTGCGCGTCTTCGAGCGCGGCAGTG
GCGAAACGCTCGCTTGCGGCACCGGCGCCTGCGCGGCGGTGGCCCACGGTATCCGCCGTGGCTGGCTCGAGAGCCCGGTC
GACGTGCACCTGCCCGGCGGCACGCTGACCCTGGCCTGGCCCGCGCCGGATGCGTCCCTGATGATGACCGGCCCGGCCAC
GAGCGTCTTCGAAGGACGTATCGCGTTGCCCTGA

Upstream 100 bases:

>100_bases
CCGAACTCATGGTCGATGGCGACAACGTGCAGGTGGTGCGCGAGCGCGAGACACTCGATGCCTTGTGGGCCGGCGAACAC
CGTCTGGCGGAGACGTCCTG

Downstream 100 bases:

>100_bases
CGATTCTGGCCGGAGCACGCGGATGTCGCCCGCGGGCAAGCGAAAAACAACACGCTAGGCGTCCCTGAAAAGCCGGCTGC
GCGACGGCGCTTTTCAGGCC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLLHFTKMHGLGNDFVVIDLITQRARLDTERIRQLADRHVGIGFDQLLLVEPPRDPEMDFRYRIFNADGNEVENCGNGAR
CFARFVRDNRLTHKREIRVETQAGPLTLRVEDDERIRVDMGVPRFAPEVVPFDAPADAPAHCLEVDGETWDIGAVSMGNP
HAVLRVDDVAQAPVARLGPLIERHPRFPRGVNVGFLQVVSRHEARLRVFERGSGETLACGTGACAAVAHGIRRGWLESPV
DVHLPGGTLTLAWPAPDASLMMTGPATSVFEGRIALP

Sequences:

>Translated_277_residues
MLLHFTKMHGLGNDFVVIDLITQRARLDTERIRQLADRHVGIGFDQLLLVEPPRDPEMDFRYRIFNADGNEVENCGNGAR
CFARFVRDNRLTHKREIRVETQAGPLTLRVEDDERIRVDMGVPRFAPEVVPFDAPADAPAHCLEVDGETWDIGAVSMGNP
HAVLRVDDVAQAPVARLGPLIERHPRFPRGVNVGFLQVVSRHEARLRVFERGSGETLACGTGACAAVAHGIRRGWLESPV
DVHLPGGTLTLAWPAPDASLMMTGPATSVFEGRIALP
>Mature_277_residues
MLLHFTKMHGLGNDFVVIDLITQRARLDTERIRQLADRHVGIGFDQLLLVEPPRDPEMDFRYRIFNADGNEVENCGNGAR
CFARFVRDNRLTHKREIRVETQAGPLTLRVEDDERIRVDMGVPRFAPEVVPFDAPADAPAHCLEVDGETWDIGAVSMGNP
HAVLRVDDVAQAPVARLGPLIERHPRFPRGVNVGFLQVVSRHEARLRVFERGSGETLACGTGACAAVAHGIRRGWLESPV
DVHLPGGTLTLAWPAPDASLMMTGPATSVFEGRIALP

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=58.3941605839416, Blast_Score=327, Evalue=6e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_CHRSD (Q1QSV1)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_575156.1
- ProteinModelPortal:   Q1QSV1
- SMR:   Q1QSV1
- STRING:   Q1QSV1
- GeneID:   4028754
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_3113
- NMPDR:   fig|290398.4.peg.3022
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- BioCyc:   CSAL290398:CSAL_3113-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30583; Mature: 30583

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHFTKMHGLGNDFVVIDLITQRARLDTERIRQLADRHVGIGFDQLLLVEPPRDPEMDF
CEEEEEECCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
RYRIFNADGNEVENCGNGARCFARFVRDNRLTHKREIRVETQAGPLTLRVEDDERIRVDM
EEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCEEEEEE
GVPRFAPEVVPFDAPADAPAHCLEVDGETWDIGAVSMGNPHAVLRVDDVAQAPVARLGPL
CCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEEECCHHHCCHHHHHHH
IERHPRFPRGVNVGFLQVVSRHEARLRVFERGSGETLACGTGACAAVAHGIRRGWLESPV
HHHCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCC
DVHLPGGTLTLAWPAPDASLMMTGPATSVFEGRIALP
EEEECCCEEEEEECCCCCEEEEECCCCHHCCCEEECC
>Mature Secondary Structure
MLLHFTKMHGLGNDFVVIDLITQRARLDTERIRQLADRHVGIGFDQLLLVEPPRDPEMDF
CEEEEEECCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
RYRIFNADGNEVENCGNGARCFARFVRDNRLTHKREIRVETQAGPLTLRVEDDERIRVDM
EEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCEEEEEE
GVPRFAPEVVPFDAPADAPAHCLEVDGETWDIGAVSMGNPHAVLRVDDVAQAPVARLGPL
CCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEEECCHHHCCHHHHHHH
IERHPRFPRGVNVGFLQVVSRHEARLRVFERGSGETLACGTGACAAVAHGIRRGWLESPV
HHHCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCC
DVHLPGGTLTLAWPAPDASLMMTGPATSVFEGRIALP
EEEECCCEEEEEECCCCCEEEEECCCCHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA