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The map label for this gene is ilvE [H]

Identifier: 92115035

GI number: 92115035

Start: 3252283

End: 3253383

Strand: Direct

Name: ilvE [H]

Synonym: Csal_2919

Alternate gene names: 92115035

Gene position: 3252283-3253383 (Clockwise)

Preceding gene: 92115034

Following gene: 92115036

Centisome position: 87.98

GC content: 68.21

Gene sequence:

>1101_bases
GTGACCACTTCAGGCTTCGACCTACGGCCTAACGCCCACCCCACGGATGCCGCCCTGCGCGAGACGATTCTCGAGAATCC
CGGCTTCGGGCGTCACTTCAGCGACCACATGGCCCACATTCGCTGGACGGTCGATGCCGGCTGGCACGGCCACGAAGTCC
GCCCCTACGGGCCGCTGACGCTGGACCCCGCGGCGGCCGTGCTGCACTACGCCCAGGAGATCTTCGAAGGCATCAAGGCC
TATCGGCATGCCGACGGCTCGGTGTGGACCTTCCGCCCCGAGAAGAACGCCGAGCGTTTCCGTGCCTCCGCGCGTCGCCT
GGCGCTGCCGGAACTCTCCGACGAGGACTTCATCGGGTCGCTCAGGGCGCTGCTCGCCCAGGACAACGCCTGGGTGCCGA
CGCCGGCCAGCGCCGCCGAAGAATCCAGCCTCTACCTGCGTCCGTTCATGATCGCCAGCGAGACCTTCCTGGGCGTGCGT
CCGTCCCATGAGGTCGACTACTACGTCATCGCCTCGCCGGCGGCGGCCTACTTCAAGGGCGGGGTCGACCCGGTCTCCAT
CTGGCTGTCCTCGCATTACAAGCGGGCCGCGCCGGGGGGCACGGGCTATGCCAAGTGCGGCGGCAACTACGCGGCTTCGC
TGGCGGCCCAGAAGGAAGCCGAGGCCCACGGCTGCGCCCAGGTGGCCTTCCTCGACGCCGCCGAGAACAAGTGGATCGAG
GAACTCGGCGGCATGAACCTGTTCTTCGTGTTCAAGGACGGGCGCATCGTCACGCCCAAGCTGACCGGCACCATCCTCGA
AGGCGTCACCCGCGACTCCATCCTGGAGCTCGCCAAGGACCAGGGCCTGACGCCCGAGGAACGCCACATCAGCATCGACG
AGTGGCGTGCCGGCGCAGCCTCCGGCGAGATCACCGAGGTCTTCGCCTGCGGCACCGCCGCCGTCATCACCCCGGTCGGC
GAGCTGCGCAGCGAAGACGAGAACATTCGCCTCAAGGCGGACAGCGGTGGCGAGGTCGGCAAGCGCCTGCGCAAGGAACT
CCTCGACCTGCAGTACGGCCGCAGCGAGGACACGCGCAACTGGCTGACGCGTCTGGCCTGA

Upstream 100 bases:

>100_bases
TTTGCACCCGCGTTCTTCGCCGATGGCGCGCAACGTCAACGCGGTATTGCCCAGCGCAGGCATTTTGTAGACCCCCTCTT
GGATGCAACGGAGAACTTCA

Downstream 100 bases:

>100_bases
CGCACCCGCGCACGACGGCACGGCCCGGCCGTGCCGTCCCCTTTTCCACGGCAAGGAGGTCACGATGACGAAAGTGATGC
TGATTACCGGCGCCGGTCGC

Product: branched chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTTSGFDLRPNAHPTDAALRETILENPGFGRHFSDHMAHIRWTVDAGWHGHEVRPYGPLTLDPAAAVLHYAQEIFEGIKA
YRHADGSVWTFRPEKNAERFRASARRLALPELSDEDFIGSLRALLAQDNAWVPTPASAAEESSLYLRPFMIASETFLGVR
PSHEVDYYVIASPAAAYFKGGVDPVSIWLSSHYKRAAPGGTGYAKCGGNYAASLAAQKEAEAHGCAQVAFLDAAENKWIE
ELGGMNLFFVFKDGRIVTPKLTGTILEGVTRDSILELAKDQGLTPEERHISIDEWRAGAASGEITEVFACGTAAVITPVG
ELRSEDENIRLKADSGGEVGKRLRKELLDLQYGRSEDTRNWLTRLA

Sequences:

>Translated_366_residues
MTTSGFDLRPNAHPTDAALRETILENPGFGRHFSDHMAHIRWTVDAGWHGHEVRPYGPLTLDPAAAVLHYAQEIFEGIKA
YRHADGSVWTFRPEKNAERFRASARRLALPELSDEDFIGSLRALLAQDNAWVPTPASAAEESSLYLRPFMIASETFLGVR
PSHEVDYYVIASPAAAYFKGGVDPVSIWLSSHYKRAAPGGTGYAKCGGNYAASLAAQKEAEAHGCAQVAFLDAAENKWIE
ELGGMNLFFVFKDGRIVTPKLTGTILEGVTRDSILELAKDQGLTPEERHISIDEWRAGAASGEITEVFACGTAAVITPVG
ELRSEDENIRLKADSGGEVGKRLRKELLDLQYGRSEDTRNWLTRLA
>Mature_365_residues
TTSGFDLRPNAHPTDAALRETILENPGFGRHFSDHMAHIRWTVDAGWHGHEVRPYGPLTLDPAAAVLHYAQEIFEGIKAY
RHADGSVWTFRPEKNAERFRASARRLALPELSDEDFIGSLRALLAQDNAWVPTPASAAEESSLYLRPFMIASETFLGVRP
SHEVDYYVIASPAAAYFKGGVDPVSIWLSSHYKRAAPGGTGYAKCGGNYAASLAAQKEAEAHGCAQVAFLDAAENKWIEE
LGGMNLFFVFKDGRIVTPKLTGTILEGVTRDSILELAKDQGLTPEERHISIDEWRAGAASGEITEVFACGTAAVITPVGE
LRSEDENIRLKADSGGEVGKRLRKELLDLQYGRSEDTRNWLTRLA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=338, Percent_Identity=39.3491124260355, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI296010906, Length=338, Percent_Identity=39.3491124260355, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI38176287, Length=338, Percent_Identity=39.3491124260355, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI50658084, Length=332, Percent_Identity=38.5542168674699, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI296010902, Length=338, Percent_Identity=34.9112426035503, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI296010900, Length=348, Percent_Identity=34.4827586206897, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI258614015, Length=288, Percent_Identity=37.8472222222222, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI48994963, Length=307, Percent_Identity=33.5504885993485, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=339, Percent_Identity=39.2330383480826, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17565728, Length=345, Percent_Identity=36.231884057971, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322002, Length=379, Percent_Identity=39.0501319261214, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322608, Length=346, Percent_Identity=40.1734104046243, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24641779, Length=340, Percent_Identity=39.1176470588235, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40183; Mature: 40052

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSGFDLRPNAHPTDAALRETILENPGFGRHFSDHMAHIRWTVDAGWHGHEVRPYGPLT
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCEEEEEEEECCCCCCCCCCCCCCE
LDPAAAVLHYAQEIFEGIKAYRHADGSVWTFRPEKNAERFRASARRLALPELSDEDFIGS
ECHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHH
LRALLAQDNAWVPTPASAAEESSLYLRPFMIASETFLGVRPSHEVDYYVIASPAAAYFKG
HHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCEECCCCCCCCCEEEEECCHHHHHCC
GVDPVSIWLSSHYKRAAPGGTGYAKCGGNYAASLAAQKEAEAHGCAQVAFLDAAENKWIE
CCCHHHHHHHHCCHHCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHH
ELGGMNLFFVFKDGRIVTPKLTGTILEGVTRDSILELAKDQGLTPEERHISIDEWRAGAA
HHCCCEEEEEEECCEEECCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCHHHHCCCCC
SGEITEVFACGTAAVITPVGELRSEDENIRLKADSGGEVGKRLRKELLDLQYGRSEDTRN
CCCHHEEEECCCHHHHCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHH
WLTRLA
HHHHCC
>Mature Secondary Structure 
TTSGFDLRPNAHPTDAALRETILENPGFGRHFSDHMAHIRWTVDAGWHGHEVRPYGPLT
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCEEEEEEEECCCCCCCCCCCCCCE
LDPAAAVLHYAQEIFEGIKAYRHADGSVWTFRPEKNAERFRASARRLALPELSDEDFIGS
ECHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHH
LRALLAQDNAWVPTPASAAEESSLYLRPFMIASETFLGVRPSHEVDYYVIASPAAAYFKG
HHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCEECCCCCCCCCEEEEECCHHHHHCC
GVDPVSIWLSSHYKRAAPGGTGYAKCGGNYAASLAAQKEAEAHGCAQVAFLDAAENKWIE
CCCHHHHHHHHCCHHCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHH
ELGGMNLFFVFKDGRIVTPKLTGTILEGVTRDSILELAKDQGLTPEERHISIDEWRAGAA
HHCCCEEEEEEECCEEECCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCHHHHCCCCC
SGEITEVFACGTAAVITPVGELRSEDENIRLKADSGGEVGKRLRKELLDLQYGRSEDTRN
CCCHHEEEECCCHHHHCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHH
WLTRLA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]