Click here to switch to the map view.

The map label for this gene is def

Identifier: 92114984

GI number: 92114984

Start: 3198658

End: 3199170

Strand: Direct

Name: def

Synonym: Csal_2867

Alternate gene names: 92114984

Gene position: 3198658-3199170 (Clockwise)

Preceding gene: 92114978

Following gene: 92114985

Centisome position: 86.53

GC content: 62.96

Gene sequence:

>513_bases
ATGGCCAAGCTGACCATTCTCGAATTTCCCGACGAGCGCCTGCGCACCAAGGCCGCGCCCGTCGAGACCGTCGACGACGA
GACGCGCAAGCTCGTCGACGACATGCTAGAGACCATGTACGACGCCCAGGGCATCGGATTGGCCGCCACGCAGGTCGACG
TGCATCGTCGCGTCATCGTGATGGATGTCAGCGATGATCGCTCGCAGCCGCGCGTGCTGATCAATCCCGAATACACGCCA
TTGGGCGACGAACGCGAGCCGATGCAGGAAGGCTGCCTGTCGATCCCCGAGTATTATGCCGAAGTGCCGCGCGCCCTGCG
CGTGTCGCTGAAGGCGCTGGATCGCGATGGCAATCCCTACGAGCTGGAGGCCGACGGCCTGCTCGCGCACTGCATCCAGC
ACGAATACGATCATCTCGAAGGCGTGCTGTTCGTCGACTACCTCTCGCCGCTCAAGCGCGACCGGGTACTGAAAAAGATG
CAGAAACGCCACCGGGCCATGCAAGACGCCTGA

Upstream 100 bases:

>100_bases
CCTGAGGGCTCATTCTCATGGCCCTGGTTCCGTTACCGTCGACCGCGCATTACACTAGAGGACAAACTTCACGTGACAGC
ATAACGGTTTTCTCAACGCC

Downstream 100 bases:

>100_bases
GCCGGCACCGCGGTCGTTCGCCCGGATGCCATGCGGGCCGGCAATCGCCGGCCCGTCGCGTTTGCCGCCCCGGCGCCGCA
CCCGCCTTCGCCACGCACCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MAKLTILEFPDERLRTKAAPVETVDDETRKLVDDMLETMYDAQGIGLAATQVDVHRRVIVMDVSDDRSQPRVLINPEYTP
LGDEREPMQEGCLSIPEYYAEVPRALRVSLKALDRDGNPYELEADGLLAHCIQHEYDHLEGVLFVDYLSPLKRDRVLKKM
QKRHRAMQDA

Sequences:

>Translated_170_residues
MAKLTILEFPDERLRTKAAPVETVDDETRKLVDDMLETMYDAQGIGLAATQVDVHRRVIVMDVSDDRSQPRVLINPEYTP
LGDEREPMQEGCLSIPEYYAEVPRALRVSLKALDRDGNPYELEADGLLAHCIQHEYDHLEGVLFVDYLSPLKRDRVLKKM
QKRHRAMQDA
>Mature_169_residues
AKLTILEFPDERLRTKAAPVETVDDETRKLVDDMLETMYDAQGIGLAATQVDVHRRVIVMDVSDDRSQPRVLINPEYTPL
GDEREPMQEGCLSIPEYYAEVPRALRVSLKALDRDGNPYELEADGLLAHCIQHEYDHLEGVLFVDYLSPLKRDRVLKKMQ
KRHRAMQDA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=31.4814814814815, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=60.6060606060606, Blast_Score=199, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24645728, Length=154, Percent_Identity=30.5194805194805, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=33.1210191082803, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CHRSD (Q1QTJ5)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574912.1
- ProteinModelPortal:   Q1QTJ5
- SMR:   Q1QTJ5
- STRING:   Q1QTJ5
- GeneID:   4028669
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2867
- NMPDR:   fig|290398.4.peg.2561
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- BioCyc:   CSAL290398:CSAL_2867-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19535; Mature: 19403

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTILEFPDERLRTKAAPVETVDDETRKLVDDMLETMYDAQGIGLAATQVDVHRRVIV
CCCEEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHEECCCCEEEE
MDVSDDRSQPRVLINPEYTPLGDEREPMQEGCLSIPEYYAEVPRALRVSLKALDRDGNPY
EECCCCCCCCEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCE
ELEADGLLAHCIQHEYDHLEGVLFVDYLSPLKRDRVLKKMQKRHRAMQDA
EECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKLTILEFPDERLRTKAAPVETVDDETRKLVDDMLETMYDAQGIGLAATQVDVHRRVIV
CCEEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHEECCCCEEEE
MDVSDDRSQPRVLINPEYTPLGDEREPMQEGCLSIPEYYAEVPRALRVSLKALDRDGNPY
EECCCCCCCCEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCE
ELEADGLLAHCIQHEYDHLEGVLFVDYLSPLKRDRVLKKMQKRHRAMQDA
EECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA