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The map label for this gene is astE [H]

Identifier: 92114898

GI number: 92114898

Start: 3112474

End: 3113454

Strand: Direct

Name: astE [H]

Synonym: Csal_2780

Alternate gene names: 92114898

Gene position: 3112474-3113454 (Clockwise)

Preceding gene: 92114897

Following gene: 92114899

Centisome position: 84.2

GC content: 69.01

Gene sequence:

>981_bases
ATGCTCGACGACTGGCTCGCCTTCAGCCTCGACGGTACGCCGCCGGCCGTCGATCGGGGCACGGTGCCGGGCGGCCGCTA
CCGTCTGCTGGCGCCGGGGCTGCTGGAGCTGACTCCCGACGCCCCCGCCCCCCATGCCCACGCCTGCGTCATATCGGCGG
GCATCCATGGCAACGAGACCGCGCCCATCGAGCTCGTCGGCGAATGGCTGGCACGCCTGGAAGCCGGCCGCTCGACGCTG
GGCGCGCCGGTGCTGGTGATACTCGGCAACCTGGAAGCGATTCGCCGCGGGGAGCGCTTCTGCACCACGAATCTCAACCG
CCTGTTTCGGCGTGACCTTGACCTCTCGGGCCCCGAGCCCGACCGCGCCCGCGAGTTGATGAGCGCGGTGGACGCCTTCT
TTGCCCGCCATCGCTTGCCGCGCCTGCACTACGACATGCACACCGCGATTCGCGAGAGTCGCTATCCGTGTTTCGCCGTC
GACCCCCACGCCGAGACGCGCACGACGCACGAACAATGGCGCTGGCTGGCCGCCGCCGGCATGCAGGCGATCCTGCATCA
ACACCGCCATAGCTGGACCTTTTCGCATTATTCGAAGCATTATCACGGCGCGCAGGCCTTCACCCTGGAGCTCGGCAAGG
TACGCCCCTTCGGCGAGAACGATCTGGCCGCGCTGACCGGCATGAGCCGCCTGCTCGACGCCCTGCTCGAGGGGCGCCCG
GCCCCCGAGGGCGAGATCGACAGCATGCGCTTCTTCGGTGTCGTCACCGAGCTGATGCGCCAGTCGAGCGACTTCCAGTT
GCGCTTTGCCGAGACGACGCCCAACTTCACCGAGTTCGCCGTGGGCACGCCACTGGCGCACGATGGCGAGGCCGGCGACA
TCCGCGTCGAGGATGCCCCGGTCGCGGTGGTGTTTCCCAATGCGCAGGTGGAAGTCGGTGCACGTGCCGCGCTCCTGGTG
CGTGCCTGCCCGCCGCCGTAG

Upstream 100 bases:

>100_bases
GTCACGACGGGGCTCTTGAGCAGGATTCCTCCACCATCGGCATGCATGCACCCGATGTCTTCACCAAGGGGTGCGGTGCG
CACCGCGAGGAGCGTTGAGC

Downstream 100 bases:

>100_bases
ACGTGCCATTTCATTCGCCGCGCCGCGTTTCGCACACGGCGCTCATCACGACAACACTCACAACGACAACAACGACAGGA
CGATCTCCCATGCCCAAAAC

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLDDWLAFSLDGTPPAVDRGTVPGGRYRLLAPGLLELTPDAPAPHAHACVISAGIHGNETAPIELVGEWLARLEAGRSTL
GAPVLVILGNLEAIRRGERFCTTNLNRLFRRDLDLSGPEPDRARELMSAVDAFFARHRLPRLHYDMHTAIRESRYPCFAV
DPHAETRTTHEQWRWLAAAGMQAILHQHRHSWTFSHYSKHYHGAQAFTLELGKVRPFGENDLAALTGMSRLLDALLEGRP
APEGEIDSMRFFGVVTELMRQSSDFQLRFAETTPNFTEFAVGTPLAHDGEAGDIRVEDAPVAVVFPNAQVEVGARAALLV
RACPPP

Sequences:

>Translated_326_residues
MLDDWLAFSLDGTPPAVDRGTVPGGRYRLLAPGLLELTPDAPAPHAHACVISAGIHGNETAPIELVGEWLARLEAGRSTL
GAPVLVILGNLEAIRRGERFCTTNLNRLFRRDLDLSGPEPDRARELMSAVDAFFARHRLPRLHYDMHTAIRESRYPCFAV
DPHAETRTTHEQWRWLAAAGMQAILHQHRHSWTFSHYSKHYHGAQAFTLELGKVRPFGENDLAALTGMSRLLDALLEGRP
APEGEIDSMRFFGVVTELMRQSSDFQLRFAETTPNFTEFAVGTPLAHDGEAGDIRVEDAPVAVVFPNAQVEVGARAALLV
RACPPP
>Mature_326_residues
MLDDWLAFSLDGTPPAVDRGTVPGGRYRLLAPGLLELTPDAPAPHAHACVISAGIHGNETAPIELVGEWLARLEAGRSTL
GAPVLVILGNLEAIRRGERFCTTNLNRLFRRDLDLSGPEPDRARELMSAVDAFFARHRLPRLHYDMHTAIRESRYPCFAV
DPHAETRTTHEQWRWLAAAGMQAILHQHRHSWTFSHYSKHYHGAQAFTLELGKVRPFGENDLAALTGMSRLLDALLEGRP
APEGEIDSMRFFGVVTELMRQSSDFQLRFAETTPNFTEFAVGTPLAHDGEAGDIRVEDAPVAVVFPNAQVEVGARAALLV
RACPPP

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate [H]

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788040, Length=322, Percent_Identity=39.7515527950311, Blast_Score=204, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007036
- InterPro:   IPR016681 [H]

Pfam domain/function: PF04952 AstE_AspA [H]

EC number: =3.5.1.96 [H]

Molecular weight: Translated: 36000; Mature: 36000

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDWLAFSLDGTPPAVDRGTVPGGRYRLLAPGLLELTPDAPAPHAHACVISAGIHGNET
CCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEECCCCCCC
APIELVGEWLARLEAGRSTLGAPVLVILGNLEAIRRGERFCTTNLNRLFRRDLDLSGPEP
CHHHHHHHHHHHHHHCCHHCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCH
DRARELMSAVDAFFARHRLPRLHYDMHTAIRESRYPCFAVDPHAETRTTHEQWRWLAAAG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
MQAILHQHRHSWTFSHYSKHYHGAQAFTLELGKVRPFGENDLAALTGMSRLLDALLEGRP
HHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
APEGEIDSMRFFGVVTELMRQSSDFQLRFAETTPNFTEFAVGTPLAHDGEAGDIRVEDAP
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCEEECCCC
VAVVFPNAQVEVGARAALLVRACPPP
EEEEECCCCEECCCCEEEEEECCCCC
>Mature Secondary Structure
MLDDWLAFSLDGTPPAVDRGTVPGGRYRLLAPGLLELTPDAPAPHAHACVISAGIHGNET
CCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEECCCCCCC
APIELVGEWLARLEAGRSTLGAPVLVILGNLEAIRRGERFCTTNLNRLFRRDLDLSGPEP
CHHHHHHHHHHHHHHCCHHCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCH
DRARELMSAVDAFFARHRLPRLHYDMHTAIRESRYPCFAVDPHAETRTTHEQWRWLAAAG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
MQAILHQHRHSWTFSHYSKHYHGAQAFTLELGKVRPFGENDLAALTGMSRLLDALLEGRP
HHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
APEGEIDSMRFFGVVTELMRQSSDFQLRFAETTPNFTEFAVGTPLAHDGEAGDIRVEDAP
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCEEECCCC
VAVVFPNAQVEVGARAALLVRACPPP
EEEEECCCCEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA