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The map label for this gene is ghrA [H]

Identifier: 92114852

GI number: 92114852

Start: 3066541

End: 3067473

Strand: Direct

Name: ghrA [H]

Synonym: Csal_2734

Alternate gene names: 92114852

Gene position: 3066541-3067473 (Clockwise)

Preceding gene: 92114851

Following gene: 92114860

Centisome position: 82.95

GC content: 68.6

Gene sequence:

>933_bases
ATGCGCATCCTGATTCATGCCGACGACGCCGCGCTCTGGAAGGAACGACTCGCCGCGAGGCTGCCCGACGCCACCTTCGC
CATCAGCGGCGAAACCCGCGATTTCCAGGCCGACTACCTGGTGGCATGGAAGCCGCCGGCCTCGCTGTTCGACGCTCAGC
CCGCGCTCAAGGGGATCGTCAACCTCGGCGCCGGCGTGGATGCGCTGCTCAACAATCCCTCGCTCCCCCAGGACATTCCC
ATCGTCAAGCTGCGCGATGCCGGCATGGGCGAGTTGATCGCCGATTATGTGCGCTATGGCGTGCTCCACTTCCAGCGCGA
CTTCGACCGCTACCGTCGCCAGCAGCACCGTGCCGAGTGGCGGGAACATGCCGTCACGCCCAAGCGCGAGTGGCGTGTGG
GGGTGCTCGGCCTGGGTGCCATCGGACTGCACGTCGCCCAGCGTCTCGCCGAAGACGGCTACCCGGTGCATGGCTGGAGC
CGTTCGCCCAAGGCCATCAATGGCATCACCTGCCATCACGGCGACGATGGCCTGACGACCCTGCTGGAACAGGTCCATAC
CCTGGTCACGCTGTTGCCCGACACCCCGGCCACGCGACACGTCATCAATCACGAGACGCTGGCCGCGCTGCCCCACGGCG
CCGGCCTGATCAATCCCGGGCGCGGCACGCTGATCGACGAGGCCGCCCTGCTCGAGGCGCTGGGCACCGGGGAGGAAGAA
AACGGCCGGCTGCGCGGCGCCATGCTCGACGCCTTCCCCACGGAGCCGCTGCCCGGCGACAGTCCCTTGTGGTCGCATCC
GCGCCTGTGGATCACGCCGCACATGGCCGGACCGACCCCCGTCGACGAGGCCGCCGAGCAGGTCGCCAAGGCTCTCGGCG
CCATGCATGCCGGCGAGGCCATCGACACCGTGGACCCCAAGGCGGGTTATTGA

Upstream 100 bases:

>100_bases
GTATGACGCGATGACGCACCGGGCGGTTGCCCGCCGTCACGCACGCCACTCATTCATCATCGATCCGCACGCCAATCGAT
TCGTACGCCACGAGGTTTTC

Downstream 100 bases:

>100_bases
GCCGCGAGCGCCTCGGCGTCCTCGCGCCCCGTCATCGGCACCCGTGCGCCCCGGGCATCGAAGACATGGCAATGAGCCAC
CGGCAACGCCAGCGACAAGC

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRILIHADDAALWKERLAARLPDATFAISGETRDFQADYLVAWKPPASLFDAQPALKGIVNLGAGVDALLNNPSLPQDIP
IVKLRDAGMGELIADYVRYGVLHFQRDFDRYRRQQHRAEWREHAVTPKREWRVGVLGLGAIGLHVAQRLAEDGYPVHGWS
RSPKAINGITCHHGDDGLTTLLEQVHTLVTLLPDTPATRHVINHETLAALPHGAGLINPGRGTLIDEAALLEALGTGEEE
NGRLRGAMLDAFPTEPLPGDSPLWSHPRLWITPHMAGPTPVDEAAEQVAKALGAMHAGEAIDTVDPKAGY

Sequences:

>Translated_310_residues
MRILIHADDAALWKERLAARLPDATFAISGETRDFQADYLVAWKPPASLFDAQPALKGIVNLGAGVDALLNNPSLPQDIP
IVKLRDAGMGELIADYVRYGVLHFQRDFDRYRRQQHRAEWREHAVTPKREWRVGVLGLGAIGLHVAQRLAEDGYPVHGWS
RSPKAINGITCHHGDDGLTTLLEQVHTLVTLLPDTPATRHVINHETLAALPHGAGLINPGRGTLIDEAALLEALGTGEEE
NGRLRGAMLDAFPTEPLPGDSPLWSHPRLWITPHMAGPTPVDEAAEQVAKALGAMHAGEAIDTVDPKAGY
>Mature_310_residues
MRILIHADDAALWKERLAARLPDATFAISGETRDFQADYLVAWKPPASLFDAQPALKGIVNLGAGVDALLNNPSLPQDIP
IVKLRDAGMGELIADYVRYGVLHFQRDFDRYRRQQHRAEWREHAVTPKREWRVGVLGLGAIGLHVAQRLAEDGYPVHGWS
RSPKAINGITCHHGDDGLTTLLEQVHTLVTLLPDTPATRHVINHETLAALPHGAGLINPGRGTLIDEAALLEALGTGEEE
NGRLRGAMLDAFPTEPLPGDSPLWSHPRLWITPHMAGPTPVDEAAEQVAKALGAMHAGEAIDTVDPKAGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=302, Percent_Identity=25.8278145695364, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI145580578, Length=243, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4557499, Length=243, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI61743967, Length=248, Percent_Identity=30.241935483871, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4557497, Length=243, Percent_Identity=30.0411522633745, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI145580575, Length=241, Percent_Identity=29.045643153527, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI87081824, Length=312, Percent_Identity=38.1410256410256, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI87082289, Length=253, Percent_Identity=30.0395256916996, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789279, Length=183, Percent_Identity=29.5081967213115, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=153, Percent_Identity=32.6797385620915, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321253, Length=180, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324055, Length=244, Percent_Identity=27.0491803278689, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19921140, Length=163, Percent_Identity=35.5828220858896, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33743; Mature: 33743

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILIHADDAALWKERLAARLPDATFAISGETRDFQADYLVAWKPPASLFDAQPALKGIV
CEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHCCCHHHHHHH
NLGAGVDALLNNPSLPQDIPIVKLRDAGMGELIADYVRYGVLHFQRDFDRYRRQQHRAEW
HCCCCHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REHAVTPKREWRVGVLGLGAIGLHVAQRLAEDGYPVHGWSRSPKAINGITCHHGDDGLTT
HHHCCCCCHHCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHH
LLEQVHTLVTLLPDTPATRHVINHETLAALPHGAGLINPGRGTLIDEAALLEALGTGEEE
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
NGRLRGAMLDAFPTEPLPGDSPLWSHPRLWITPHMAGPTPVDEAAEQVAKALGAMHAGEA
CCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
IDTVDPKAGY
CCCCCCCCCC
>Mature Secondary Structure
MRILIHADDAALWKERLAARLPDATFAISGETRDFQADYLVAWKPPASLFDAQPALKGIV
CEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHCCCHHHHHHH
NLGAGVDALLNNPSLPQDIPIVKLRDAGMGELIADYVRYGVLHFQRDFDRYRRQQHRAEW
HCCCCHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REHAVTPKREWRVGVLGLGAIGLHVAQRLAEDGYPVHGWSRSPKAINGITCHHGDDGLTT
HHHCCCCCHHCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHH
LLEQVHTLVTLLPDTPATRHVINHETLAALPHGAGLINPGRGTLIDEAALLEALGTGEEE
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
NGRLRGAMLDAFPTEPLPGDSPLWSHPRLWITPHMAGPTPVDEAAEQVAKALGAMHAGEA
CCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
IDTVDPKAGY
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]