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The map label for this gene is gcvA [H]

Identifier: 92114835

GI number: 92114835

Start: 3046126

End: 3047052

Strand: Direct

Name: gcvA [H]

Synonym: Csal_2717

Alternate gene names: 92114835

Gene position: 3046126-3047052 (Clockwise)

Preceding gene: 92114832

Following gene: 92114837

Centisome position: 82.4

GC content: 66.24

Gene sequence:

>927_bases
ATGAATCGACTCGGCAAGGCCCTGCCCCCGCTCGCCAGCCTGATGCCCTTCGAAGCCGCCGCACGCCTGGAAAGCTTTTC
CCGCGCCGCCGACGAGCTGCATCTCACCCAGGCCGCCATCAGTCGGCAGATTCGGGCACTGGAAGACGATCTCGGCGTCG
CCCTGTTCGAACGCCGAAACCGGGGCGTGTTTCTCACCGCTGCCGGCCGTGAATTCGGACGCACGGTGAGCGCCGCACTG
GAGGGGATTGCCGCGCAGGCCAATGCCCTGCGTCAGGAAGTGCATGACGGCGTCGTGGTGCTGTTCTGTCAGCTCTGCGA
GGCATTCTACTGGGTGATGCCCAGACTCGCCTCGCTCAACCGCCGGCATCCGGAACTCGAGGTGCGCCTGGTGACCTCGA
CGCGCCCCGTGGTCGAGCACCATGACTATTTCGATGTCGCGCTGCAGACGAGCGGCCGCCCCAGCGGCAGCCATGCGTTG
GCTTTCACCGCCTCCGACGAGATTTTCCCCATCTGCAGCCCCGGTTACCTGGCCGCCCAGCCGCGCCCCATCACGCTGGC
GTCCCTGCCCGAGCACTGCCTGCTCCATCACGAGGCCGATCCGCCGGACTGGTTCGAGTGGGACAGCTGGCTGAGAGCCA
TGGGCAACTTCGCGCCGGCCCTGGGTAACGGCATGGTCTTCGACAGCTATCCGCTGGTCCTGCAGGCGGCGCTTGCGGGT
CATGGCATCGCCCTGGGCTGGCGGCGTTCCGCCGAGCACCTCATCCAGAGCGGACAGCTCGTACGTCCGCTGTCGATGTC
TCTGCCACTCGACAATGCACTGTCCGTTTACACGCGACACGGCGCCCAGCCACGCGCGGAAACGCTGGCGCTGCTGGACT
GGCTGCAACGTGAGTTTCGAAGAGACGATCAGACGACACCGGCTTGA

Upstream 100 bases:

>100_bases
GTTCTCCCTGAGCAATAAGCCGCTCAAGGGTGCGCGCCCCAGGCATGCACGACAAACGAGTTATGCGTTACATTCCATGA
GCTGAGATTATGGATCTTAT

Downstream 100 bases:

>100_bases
CAGGCATCAGCCCACCATCGCCTGCCGGTACCGGCGTCTCACAGCAACGCGACGATCACCGCTCCCACCGTGCCGCCGGT
GAGGATCAGCGCCAGCAGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNRLGKALPPLASLMPFEAAARLESFSRAADELHLTQAAISRQIRALEDDLGVALFERRNRGVFLTAAGREFGRTVSAAL
EGIAAQANALRQEVHDGVVVLFCQLCEAFYWVMPRLASLNRRHPELEVRLVTSTRPVVEHHDYFDVALQTSGRPSGSHAL
AFTASDEIFPICSPGYLAAQPRPITLASLPEHCLLHHEADPPDWFEWDSWLRAMGNFAPALGNGMVFDSYPLVLQAALAG
HGIALGWRRSAEHLIQSGQLVRPLSMSLPLDNALSVYTRHGAQPRAETLALLDWLQREFRRDDQTTPA

Sequences:

>Translated_308_residues
MNRLGKALPPLASLMPFEAAARLESFSRAADELHLTQAAISRQIRALEDDLGVALFERRNRGVFLTAAGREFGRTVSAAL
EGIAAQANALRQEVHDGVVVLFCQLCEAFYWVMPRLASLNRRHPELEVRLVTSTRPVVEHHDYFDVALQTSGRPSGSHAL
AFTASDEIFPICSPGYLAAQPRPITLASLPEHCLLHHEADPPDWFEWDSWLRAMGNFAPALGNGMVFDSYPLVLQAALAG
HGIALGWRRSAEHLIQSGQLVRPLSMSLPLDNALSVYTRHGAQPRAETLALLDWLQREFRRDDQTTPA
>Mature_308_residues
MNRLGKALPPLASLMPFEAAARLESFSRAADELHLTQAAISRQIRALEDDLGVALFERRNRGVFLTAAGREFGRTVSAAL
EGIAAQANALRQEVHDGVVVLFCQLCEAFYWVMPRLASLNRRHPELEVRLVTSTRPVVEHHDYFDVALQTSGRPSGSHAL
AFTASDEIFPICSPGYLAAQPRPITLASLPEHCLLHHEADPPDWFEWDSWLRAMGNFAPALGNGMVFDSYPLVLQAALAG
HGIALGWRRSAEHLIQSGQLVRPLSMSLPLDNALSVYTRHGAQPRAETLALLDWLQREFRRDDQTTPA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=33.1058020477816, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=29.3918918918919, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786448, Length=268, Percent_Identity=32.4626865671642, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI145693193, Length=270, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=26.7605633802817, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789440, Length=271, Percent_Identity=27.6752767527675, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787589, Length=264, Percent_Identity=23.8636363636364, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693105, Length=126, Percent_Identity=34.9206349206349, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=32.8467153284672, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787128, Length=257, Percent_Identity=24.5136186770428, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=129, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34099; Mature: 34099

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLGKALPPLASLMPFEAAARLESFSRAADELHLTQAAISRQIRALEDDLGVALFERRN
CCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGVFLTAAGREFGRTVSAALEGIAAQANALRQEVHDGVVVLFCQLCEAFYWVMPRLASLN
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
RRHPELEVRLVTSTRPVVEHHDYFDVALQTSGRPSGSHALAFTASDEIFPICSPGYLAAQ
CCCCCEEEEEEECCCCHHHCCCCEEEEEECCCCCCCCCEEEEECCCCEEECCCCCCCCCC
PRPITLASLPEHCLLHHEADPPDWFEWDSWLRAMGNFAPALGNGMVFDSYPLVLQAALAG
CCCEEHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHC
HGIALGWRRSAEHLIQSGQLVRPLSMSLPLDNALSVYTRHGAQPRAETLALLDWLQREFR
CCEEECCHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
RDDQTTPA
CCCCCCCC
>Mature Secondary Structure
MNRLGKALPPLASLMPFEAAARLESFSRAADELHLTQAAISRQIRALEDDLGVALFERRN
CCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGVFLTAAGREFGRTVSAALEGIAAQANALRQEVHDGVVVLFCQLCEAFYWVMPRLASLN
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
RRHPELEVRLVTSTRPVVEHHDYFDVALQTSGRPSGSHALAFTASDEIFPICSPGYLAAQ
CCCCCEEEEEEECCCCHHHCCCCEEEEEECCCCCCCCCEEEEECCCCEEECCCCCCCCCC
PRPITLASLPEHCLLHHEADPPDWFEWDSWLRAMGNFAPALGNGMVFDSYPLVLQAALAG
CCCEEHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHC
HGIALGWRRSAEHLIQSGQLVRPLSMSLPLDNALSVYTRHGAQPRAETLALLDWLQREFR
CCEEECCHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
RDDQTTPA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]