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The map label for this gene is glcF [H]

Identifier: 92114807

GI number: 92114807

Start: 3012552

End: 3013811

Strand: Direct

Name: glcF [H]

Synonym: Csal_2689

Alternate gene names: 92114807

Gene position: 3012552-3013811 (Clockwise)

Preceding gene: 92114806

Following gene: 92114808

Centisome position: 81.49

GC content: 67.62

Gene sequence:

>1260_bases
ATGCAGACGAATTTTTCCGACGAGGCGCTGCGCCAACCGCACATCCGCGAGGCCGACCGCGTCCTGCGATCGTGCGTGCA
CTGCGGCTTCTGCAACGCCACCTGCCCGACCTATCAACTGCTGGGCGACGAGCGCGACGGCCCGCGCGGGCGCATCTATC
TGATGAAGGAGCTGCTCGAAAGCCGCGACGATGACGACCAGGTCACCCACGAAACGCAAACCCACCTCGACCGCTGCCTG
AGCTGCCGCAACTGCGAGACCACCTGCCCCTCGGGGGTCGAGTATCACAAGCTGCTCGACATCGGGCGCGCCGAGGTGGA
CCGCCGTGTGGGACGCTCTGCCAAGGAGCGAGCCCTGCGCTACGGGCTGCGCAAGGCGCTGGTCGAGCCCCGGCGGTTTC
AAGCGTTGCTCGCCCTGGGCCAGACCTTCCGCCCGCTTGCGCCGACCTCGCTGAAGGCCAAGATCCCGCCCCGCGCGTCC
CGTGCGGGACGTACCGCGCCCACGACGCACAACCGCCGCATGCTCGTGCTCGAAGGCTGCGTGCAACCGGGGTTGTCGCC
GAACACCAATGACGCCGCGACCCGCGTTCTCGACCGGCTGGGCATTGCCCTGAGCTCGGCCCCCGCGGCAGGCTGCTGCG
GCGCCATCGACTATCATCTCAATGCCCAGGAGGCTGGCCGCGAGCGCATGCGGGCCAACATCGACGCCTGGTGGCCATAC
ATCGACCAAGGCGTCGAAGCCATCGTGCAGACTGCCAGCGGCTGCGGGGCGTTCGTCAAGGAATACGGCGACATGCTCGC
GGACGACCCGCGCTACGCCGACAAGGCCAGACACATCAGCGCCCTGGCCAAGGACCTGGTGGAAATCCTGCGGGAAGAAG
ACCTGGAAGCATTGGCGGTTCACGGCGACGCACGACTCGCCTTCCATTGCCCTTGCACGCTGCAGCACGCCCAGAAGCTG
GGCGGCGCGGTGGAAAGCGTGCTCGGCCGGCTGGGCTTCTCGCTGACGACCGTGCGCGACGGCCATCTGTGCTGCGGCTC
GGCGGGTACCTACTCGATCACGCAGCCCGCCTTGGCAACACAATTGCGCGACGACAGACTCGATGCGCTCGAGGCCGGTC
AGCCCGAGCAGATCGTCACTGCCAACATCGGCTGCCAGACTCACCTGAGCGGCGCAGGGCGCACCACCGTCCGGCACTGG
ATCGAAGTCGTCGATGAGGCGCTGACCACGGCACCGAATAACGAGGCGCGGACAGCGTAG

Upstream 100 bases:

>100_bases
CGCTTTCCGTGGTGATGGACAAATATCATCGCCAGCTCAAGGCCAAGCTCGACCCCAAGGGGATCTTCAATCCCGGGCGA
CTCTACGAGGGGCTATAGCC

Downstream 100 bases:

>100_bases
CTCGTAGCTCACATTTCCCACAGGAGACCTGCATGAAAACTCAAGCCGTACTCGAACTCAGTGACGTCAATCGGCTGCTC
GACGCCGGACAACAGGAAGC

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MQTNFSDEALRQPHIREADRVLRSCVHCGFCNATCPTYQLLGDERDGPRGRIYLMKELLESRDDDDQVTHETQTHLDRCL
SCRNCETTCPSGVEYHKLLDIGRAEVDRRVGRSAKERALRYGLRKALVEPRRFQALLALGQTFRPLAPTSLKAKIPPRAS
RAGRTAPTTHNRRMLVLEGCVQPGLSPNTNDAATRVLDRLGIALSSAPAAGCCGAIDYHLNAQEAGRERMRANIDAWWPY
IDQGVEAIVQTASGCGAFVKEYGDMLADDPRYADKARHISALAKDLVEILREEDLEALAVHGDARLAFHCPCTLQHAQKL
GGAVESVLGRLGFSLTTVRDGHLCCGSAGTYSITQPALATQLRDDRLDALEAGQPEQIVTANIGCQTHLSGAGRTTVRHW
IEVVDEALTTAPNNEARTA

Sequences:

>Translated_419_residues
MQTNFSDEALRQPHIREADRVLRSCVHCGFCNATCPTYQLLGDERDGPRGRIYLMKELLESRDDDDQVTHETQTHLDRCL
SCRNCETTCPSGVEYHKLLDIGRAEVDRRVGRSAKERALRYGLRKALVEPRRFQALLALGQTFRPLAPTSLKAKIPPRAS
RAGRTAPTTHNRRMLVLEGCVQPGLSPNTNDAATRVLDRLGIALSSAPAAGCCGAIDYHLNAQEAGRERMRANIDAWWPY
IDQGVEAIVQTASGCGAFVKEYGDMLADDPRYADKARHISALAKDLVEILREEDLEALAVHGDARLAFHCPCTLQHAQKL
GGAVESVLGRLGFSLTTVRDGHLCCGSAGTYSITQPALATQLRDDRLDALEAGQPEQIVTANIGCQTHLSGAGRTTVRHW
IEVVDEALTTAPNNEARTA
>Mature_419_residues
MQTNFSDEALRQPHIREADRVLRSCVHCGFCNATCPTYQLLGDERDGPRGRIYLMKELLESRDDDDQVTHETQTHLDRCL
SCRNCETTCPSGVEYHKLLDIGRAEVDRRVGRSAKERALRYGLRKALVEPRRFQALLALGQTFRPLAPTSLKAKIPPRAS
RAGRTAPTTHNRRMLVLEGCVQPGLSPNTNDAATRVLDRLGIALSSAPAAGCCGAIDYHLNAQEAGRERMRANIDAWWPY
IDQGVEAIVQTASGCGAFVKEYGDMLADDPRYADKARHISALAKDLVEILREEDLEALAVHGDARLAFHCPCTLQHAQKL
GGAVESVLGRLGFSLTTVRDGHLCCGSAGTYSITQPALATQLRDDRLDALEAGQPEQIVTANIGCQTHLSGAGRTTVRHW
IEVVDEALTTAPNNEARTA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=408, Percent_Identity=64.9509803921569, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1786497, Length=234, Percent_Identity=24.7863247863248, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45926; Mature: 45926

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNFSDEALRQPHIREADRVLRSCVHCGFCNATCPTYQLLGDERDGPRGRIYLMKELLE
CCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
SRDDDDQVTHETQTHLDRCLSCRNCETTCPSGVEYHKLLDIGRAEVDRRVGRSAKERALR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
YGLRKALVEPRRFQALLALGQTFRPLAPTSLKAKIPPRASRAGRTAPTTHNRRMLVLEGC
HHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHH
VQPGLSPNTNDAATRVLDRLGIALSSAPAAGCCGAIDYHLNAQEAGRERMRANIDAWWPY
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCHHHHHH
IDQGVEAIVQTASGCGAFVKEYGDMLADDPRYADKARHISALAKDLVEILREEDLEALAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
HGDARLAFHCPCTLQHAQKLGGAVESVLGRLGFSLTTVRDGHLCCGSAGTYSITQPALAT
ECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCCCCCCHHHHH
QLRDDRLDALEAGQPEQIVTANIGCQTHLSGAGRTTVRHWIEVVDEALTTAPNNEARTA
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQTNFSDEALRQPHIREADRVLRSCVHCGFCNATCPTYQLLGDERDGPRGRIYLMKELLE
CCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
SRDDDDQVTHETQTHLDRCLSCRNCETTCPSGVEYHKLLDIGRAEVDRRVGRSAKERALR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
YGLRKALVEPRRFQALLALGQTFRPLAPTSLKAKIPPRASRAGRTAPTTHNRRMLVLEGC
HHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHH
VQPGLSPNTNDAATRVLDRLGIALSSAPAAGCCGAIDYHLNAQEAGRERMRANIDAWWPY
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCHHHHHH
IDQGVEAIVQTASGCGAFVKEYGDMLADDPRYADKARHISALAKDLVEILREEDLEALAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
HGDARLAFHCPCTLQHAQKLGGAVESVLGRLGFSLTTVRDGHLCCGSAGTYSITQPALAT
ECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCCCCCCHHHHH
QLRDDRLDALEAGQPEQIVTANIGCQTHLSGAGRTTVRHWIEVVDEALTTAPNNEARTA
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]