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The map label for this gene is irgB [H]

Identifier: 92114797

GI number: 92114797

Start: 3001388

End: 3002365

Strand: Direct

Name: irgB [H]

Synonym: Csal_2679

Alternate gene names: 92114797

Gene position: 3001388-3002365 (Clockwise)

Preceding gene: 92114792

Following gene: 92114798

Centisome position: 81.19

GC content: 66.87

Gene sequence:

>978_bases
ATGCGCGCTCACCCCACAGCACGCCGATTGACGATGACGAATGGCATGTACGATCTCGATGAGCTGGCCGCCTTCGCCTC
GGTCATGGCATCCGGCAGTCTCACCGCCAGCGCCCGACAGCTCGGGGTCGCCAAGTCGACACTCAGTCGTCGCATCAGTC
AACTGGAGGAGCGCCTGGGTCAGCCCTTGCTGCGTCGCCAGGCCAATCGCCTGCTGCCCACCGAGGCCGGCACGCTCTTT
CTGGCCTACAGTCAGCAGATCCTGCGTCTCGCCGAACAGAGTCAGCGCGCCCTGGAAGACCTGCGTGCGGAAGTCAGCGG
CGAACTCACCGTGCAGGTGCACAATGCCTTTGCCCGCGGATGGTTCTCGCAGCAGATGGAGAACTTCGTCGCCGACCATC
CCGGCGTGCGTCTCGACTTGCAGACGCGCCTCACGCCGCCCACCGGGCCCGAGGACGAGGCGCTATGCGTCTGGCTGGGA
ACTGTCGACGAGTGCGGCTTGCGTCAGGAAACGCTGGGCCATATGCGGCGAGGCCTCTATGCCCACCCCGATTACCTGCG
CCGCCATGGTGCTCCCCGCCACCCTCGCGATCTGGCCGAACATGCCTGGGTCGACCTGCTCGGCACCAGCGAGCACGGCG
TGACCTTGCATCACGCCCGTGAGGGCGAGTGGGACCTCACCCTGCCCCCCTCGCGACTGCGCGTCGACCAGTACGTGCTG
CATATCGACGCCATCGCACGAGGCGGGGGGCTTGGCCTGCTGCCCCACTGGATGGCCGAGAAACGCCTGCAGCATCATCC
CGGCAGCCTGACCCCCTGCCTGGAGGAATGGGCGCCATCCTCGCTGCCCATCACGCTCCTCTACCCCTTCGGCCGTCTGC
CGCGCAAGACCGTTACCCTGCTCGAACGGATCCGCCAGGCCGTTCCGGATAGCTGGCGCTTGCATGCGCCGGCCCCCAGC
CTCCGATTTTCACGCTAG

Upstream 100 bases:

>100_bases
AAGTACGACACCACGTCATGGGACGGGGCGTTTCGCAATCCGCAACGCAACGCCATAACAGGCGCTAATGATTTTCAAAA
AGATGCAGGGTTGTGCAAGA

Downstream 100 bases:

>100_bases
ACTGACAGCCGCTACGCGACGTGACGCCTCGGGAGCCGATCGCACGGATAGCGCGTCAAATAGATAGCGTGCTAACATAG
TCGCTTGCCTTGGCGAACGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRAHPTARRLTMTNGMYDLDELAAFASVMASGSLTASARQLGVAKSTLSRRISQLEERLGQPLLRRQANRLLPTEAGTLF
LAYSQQILRLAEQSQRALEDLRAEVSGELTVQVHNAFARGWFSQQMENFVADHPGVRLDLQTRLTPPTGPEDEALCVWLG
TVDECGLRQETLGHMRRGLYAHPDYLRRHGAPRHPRDLAEHAWVDLLGTSEHGVTLHHAREGEWDLTLPPSRLRVDQYVL
HIDAIARGGGLGLLPHWMAEKRLQHHPGSLTPCLEEWAPSSLPITLLYPFGRLPRKTVTLLERIRQAVPDSWRLHAPAPS
LRFSR

Sequences:

>Translated_325_residues
MRAHPTARRLTMTNGMYDLDELAAFASVMASGSLTASARQLGVAKSTLSRRISQLEERLGQPLLRRQANRLLPTEAGTLF
LAYSQQILRLAEQSQRALEDLRAEVSGELTVQVHNAFARGWFSQQMENFVADHPGVRLDLQTRLTPPTGPEDEALCVWLG
TVDECGLRQETLGHMRRGLYAHPDYLRRHGAPRHPRDLAEHAWVDLLGTSEHGVTLHHAREGEWDLTLPPSRLRVDQYVL
HIDAIARGGGLGLLPHWMAEKRLQHHPGSLTPCLEEWAPSSLPITLLYPFGRLPRKTVTLLERIRQAVPDSWRLHAPAPS
LRFSR
>Mature_325_residues
MRAHPTARRLTMTNGMYDLDELAAFASVMASGSLTASARQLGVAKSTLSRRISQLEERLGQPLLRRQANRLLPTEAGTLF
LAYSQQILRLAEQSQRALEDLRAEVSGELTVQVHNAFARGWFSQQMENFVADHPGVRLDLQTRLTPPTGPEDEALCVWLG
TVDECGLRQETLGHMRRGLYAHPDYLRRHGAPRHPRDLAEHAWVDLLGTSEHGVTLHHAREGEWDLTLPPSRLRVDQYVL
HIDAIARGGGLGLLPHWMAEKRLQHHPGSLTPCLEEWAPSSLPITLLYPFGRLPRKTVTLLERIRQAVPDSWRLHAPAPS
LRFSR

Specific function: Transcription activation of the irgA gene. In the presence of sufficient iron, transcription of both irgA and irgB is negatively regulated by a fur-like protein. In low iron conditions, negative regulation of transcription is removed, and production of ir

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=24.3150684931507, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786401, Length=278, Percent_Identity=26.978417266187, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=25.9786476868327, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=24.3055555555556, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=25.1724137931034, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081978, Length=188, Percent_Identity=29.2553191489362, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790262, Length=256, Percent_Identity=26.171875, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36586; Mature: 36586

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAHPTARRLTMTNGMYDLDELAAFASVMASGSLTASARQLGVAKSTLSRRISQLEERLG
CCCCCCHHEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
QPLLRRQANRLLPTEAGTLFLAYSQQILRLAEQSQRALEDLRAEVSGELTVQVHNAFARG
CHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
WFSQQMENFVADHPGVRLDLQTRLTPPTGPEDEALCVWLGTVDECGLRQETLGHMRRGLY
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCHHHCCCHHHHHHHHHHCCC
AHPDYLRRHGAPRHPRDLAEHAWVDLLGTSEHGVTLHHAREGEWDLTLPPSRLRVDQYVL
CCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEECCCHHHHHHHHHHH
HIDAIARGGGLGLLPHWMAEKRLQHHPGSLTPCLEEWAPSSLPITLLYPFGRLPRKTVTL
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCCCHHHHHH
LERIRQAVPDSWRLHAPAPSLRFSR
HHHHHHHCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MRAHPTARRLTMTNGMYDLDELAAFASVMASGSLTASARQLGVAKSTLSRRISQLEERLG
CCCCCCHHEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
QPLLRRQANRLLPTEAGTLFLAYSQQILRLAEQSQRALEDLRAEVSGELTVQVHNAFARG
CHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
WFSQQMENFVADHPGVRLDLQTRLTPPTGPEDEALCVWLGTVDECGLRQETLGHMRRGLY
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCHHHCCCHHHHHHHHHHCCC
AHPDYLRRHGAPRHPRDLAEHAWVDLLGTSEHGVTLHHAREGEWDLTLPPSRLRVDQYVL
CCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEECCCHHHHHHHHHHH
HIDAIARGGGLGLLPHWMAEKRLQHHPGSLTPCLEEWAPSSLPITLLYPFGRLPRKTVTL
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCCCHHHHHH
LERIRQAVPDSWRLHAPAPSLRFSR
HHHHHHHCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1705025 [H]