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The map label for this gene is tesA [H]

Identifier: 92114738

GI number: 92114738

Start: 2933223

End: 2933807

Strand: Direct

Name: tesA [H]

Synonym: Csal_2620

Alternate gene names: 92114738

Gene position: 2933223-2933807 (Clockwise)

Preceding gene: 92114732

Following gene: 92114739

Centisome position: 79.35

GC content: 64.44

Gene sequence:

>585_bases
ATGGGGCCCGTCGCGAATGTCCATGCCGATACGTTGCTGATCGTCGGCGACAGCCTCAGCGCGGCTCACGGCATCGAACG
CGAGGCCGGCTGGGTCGCGCTGTTGCGCGAGCGCGTGGGCGATGCGCATGAAGTGGTCAATGCCAGCATCAGCGGCGACA
CCACCTCGGGCGGCGCGGCACGCCTGCCCGATCTGCTCGAGCGCCATTCTCCCGACATCGTCCTCCTCGAGCTGGGCGGC
AACGACGGCCTGCGCGGACTATCGCCGCAGCAAATGAAGCTCAATTTGCGCACGATGATCGAAAAAAGCCAAGCCGCCGA
CGCCCGTGTGCTTCTGCTCGGCATCGAGATTCCCCCCAACTATGGCGCCGCCTATACCGATGCCTTCCGCGGCGTCTTTC
GTCAACTCAGCGAAGAATACGCGGTGCCGCTGGTGCCCTTCATCCTCGACGGCGTCGCCTTGCAGGACGACATGATGCAA
GAGGACGGCATTCACCCCAACGCCGCTGCCCAGCCCACCATCCTCGACAATGTCTGGGAGAAGCTGGGTCCCATGCTGTC
GCGGGACACCGACCGGAACGAATGA

Upstream 100 bases:

>100_bases
TTTCGCTTCACTCAAGGAGTCGTCGGACATGCGTGAGAGTTCCTTTTCCTGGGCACGCTGGTGTAGACGTGGTCTGGCCG
GCATCGTGCTGGGGCTCGCC

Downstream 100 bases:

>100_bases
CCCGGGCGGGGATTCCCCGCTCCATGCGCGTGTCTTGCCTCGCATGCCGATCGGGCGTCCAGCCAGGCTAATGCTTTCCT
GACACGAAGGCGATGCCTGC

Product: arylesterase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MGPVANVHADTLLIVGDSLSAAHGIEREAGWVALLRERVGDAHEVVNASISGDTTSGGAARLPDLLERHSPDIVLLELGG
NDGLRGLSPQQMKLNLRTMIEKSQAADARVLLLGIEIPPNYGAAYTDAFRGVFRQLSEEYAVPLVPFILDGVALQDDMMQ
EDGIHPNAAAQPTILDNVWEKLGPMLSRDTDRNE

Sequences:

>Translated_194_residues
MGPVANVHADTLLIVGDSLSAAHGIEREAGWVALLRERVGDAHEVVNASISGDTTSGGAARLPDLLERHSPDIVLLELGG
NDGLRGLSPQQMKLNLRTMIEKSQAADARVLLLGIEIPPNYGAAYTDAFRGVFRQLSEEYAVPLVPFILDGVALQDDMMQ
EDGIHPNAAAQPTILDNVWEKLGPMLSRDTDRNE
>Mature_193_residues
GPVANVHADTLLIVGDSLSAAHGIEREAGWVALLRERVGDAHEVVNASISGDTTSGGAARLPDLLERHSPDIVLLELGGN
DGLRGLSPQQMKLNLRTMIEKSQAADARVLLLGIEIPPNYGAAYTDAFRGVFRQLSEEYAVPLVPFILDGVALQDDMMQE
DGIHPNAAAQPTILDNVWEKLGPMLSRDTDRNE

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=182, Percent_Identity=50, Blast_Score=186, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 20898; Mature: 20767

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPVANVHADTLLIVGDSLSAAHGIEREAGWVALLRERVGDAHEVVNASISGDTTSGGAA
CCCCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCH
RLPDLLERHSPDIVLLELGGNDGLRGLSPQQMKLNLRTMIEKSQAADARVLLLGIEIPPN
HHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCC
YGAAYTDAFRGVFRQLSEEYAVPLVPFILDGVALQDDMMQEDGIHPNAAAQPTILDNVWE
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
KLGPMLSRDTDRNE
HHHHHHCCCCCCCC
>Mature Secondary Structure 
GPVANVHADTLLIVGDSLSAAHGIEREAGWVALLRERVGDAHEVVNASISGDTTSGGAA
CCCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCH
RLPDLLERHSPDIVLLELGGNDGLRGLSPQQMKLNLRTMIEKSQAADARVLLLGIEIPPN
HHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCC
YGAAYTDAFRGVFRQLSEEYAVPLVPFILDGVALQDDMMQEDGIHPNAAAQPTILDNVWE
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
KLGPMLSRDTDRNE
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]