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The map label for this gene is rhdA [H]

Identifier: 92114646

GI number: 92114646

Start: 2834928

End: 2835758

Strand: Direct

Name: rhdA [H]

Synonym: Csal_2528

Alternate gene names: 92114646

Gene position: 2834928-2835758 (Clockwise)

Preceding gene: 92114644

Following gene: 92114647

Centisome position: 76.69

GC content: 66.43

Gene sequence:

>831_bases
ATGAGTTCCGAAGACAATCTGTTGCCGCTGATCGTCGAGCCCGAACAGCTCGCCGAGCATCTAGACGCCCCCGACCTGTT
GATCGTCGATGTGCCGCTCAAGGCGGAGAGCTACACGACGGGGCATGTCCCCGGCGCCATCTTTCTCGATCATCGACGCC
TGCTGGCGGGCGATGGCCCGGTACCCAACGACGTGCCCGATGAAGACGCCCTCTCGGCGCTGTTTTCCGAACTCGGCCTG
ACCCGCGATACGCACGTCGTGGCCTATGACGATGAAGGCGGCGGCTGGGCCGGACGCCTGCTGTGGACGCTGGAGCTCAT
CGGGCATACCCGGTATTCCTACCTCAACGGCGGCATTCACGCCTGGCGCGCGGCCGGCCTGACGCAATCCCGCGAGCCGC
ATCAGCCGCAGCCCAGCGACTACCGGGCGGAGATTCGCAACCCCGGCGTCATGATCGAGCGCGACGAGCTGCTCCAACGC
CTCGACGAGGTCCAGTTGGCGGTATGGGACGCCCGCTCACCGGCCGAATACCGGGGCGAAAAGGGCCAGAACCGGCATCT
CGGCCACATCCCGCGGGCCGTCAACATGGAATGGACCGAGGCGATGGACCGCCAGCGCGACCTGCGCATCCGCGACTATG
CCGAACTGATCACCGAACTCGACGCGCTGGGCCTGACCCCGGACATGGAGATCGTCACCCACTGCCAGAGCCACCACCGC
AGCGGCTTCACCTGGCTGGTCGGCAAGGCCCTGGGCTTCGAGAAGATGCGTGCCTATCCCGGCTCCTGGCAGGAATGGGG
CAACCGCGACGACACGCCCATCGAGCGCTGA

Upstream 100 bases:

>100_bases
CGCTCACCCCCGGTGGCCGGGATTGGCTGCACAAGCCTCGCTGCTTGTGCCATGCTCTGGTCAGCATTCACGCGCCCAAC
GTTACCCGTGGGAGAAGACC

Downstream 100 bases:

>100_bases
GCCTCGGTGACTGGACGAGGGACGCGCGTTCGATCCTCTCGTCGCCGTCAACGAAGTATTGCGACGCCCCGCCGCTGCGA
CTAAGGTGGCGGGTCCTCTT

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSSEDNLLPLIVEPEQLAEHLDAPDLLIVDVPLKAESYTTGHVPGAIFLDHRRLLAGDGPVPNDVPDEDALSALFSELGL
TRDTHVVAYDDEGGGWAGRLLWTLELIGHTRYSYLNGGIHAWRAAGLTQSREPHQPQPSDYRAEIRNPGVMIERDELLQR
LDEVQLAVWDARSPAEYRGEKGQNRHLGHIPRAVNMEWTEAMDRQRDLRIRDYAELITELDALGLTPDMEIVTHCQSHHR
SGFTWLVGKALGFEKMRAYPGSWQEWGNRDDTPIER

Sequences:

>Translated_276_residues
MSSEDNLLPLIVEPEQLAEHLDAPDLLIVDVPLKAESYTTGHVPGAIFLDHRRLLAGDGPVPNDVPDEDALSALFSELGL
TRDTHVVAYDDEGGGWAGRLLWTLELIGHTRYSYLNGGIHAWRAAGLTQSREPHQPQPSDYRAEIRNPGVMIERDELLQR
LDEVQLAVWDARSPAEYRGEKGQNRHLGHIPRAVNMEWTEAMDRQRDLRIRDYAELITELDALGLTPDMEIVTHCQSHHR
SGFTWLVGKALGFEKMRAYPGSWQEWGNRDDTPIER
>Mature_275_residues
SSEDNLLPLIVEPEQLAEHLDAPDLLIVDVPLKAESYTTGHVPGAIFLDHRRLLAGDGPVPNDVPDEDALSALFSELGLT
RDTHVVAYDDEGGGWAGRLLWTLELIGHTRYSYLNGGIHAWRAAGLTQSREPHQPQPSDYRAEIRNPGVMIERDELLQRL
DEVQLAVWDARSPAEYRGEKGQNRHLGHIPRAVNMEWTEAMDRQRDLRIRDYAELITELDALGLTPDMEIVTHCQSHHRS
GFTWLVGKALGFEKMRAYPGSWQEWGNRDDTPIER

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=244, Percent_Identity=27.0491803278689, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI61835204, Length=233, Percent_Identity=27.0386266094421, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI194473681, Length=233, Percent_Identity=27.0386266094421, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI194473668, Length=233, Percent_Identity=27.0386266094421, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI87082121, Length=275, Percent_Identity=30.1818181818182, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI87081967, Length=251, Percent_Identity=23.5059760956175, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17561888, Length=267, Percent_Identity=22.4719101123595, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115534702, Length=244, Percent_Identity=25, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 31235; Mature: 31104

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEDNLLPLIVEPEQLAEHLDAPDLLIVDVPLKAESYTTGHVPGAIFLDHRRLLAGDGP
CCCCCCEEEEEECHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCEECCCCC
VPNDVPDEDALSALFSELGLTRDTHVVAYDDEGGGWAGRLLWTLELIGHTRYSYLNGGIH
CCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
AWRAAGLTQSREPHQPQPSDYRAEIRNPGVMIERDELLQRLDEVQLAVWDARSPAEYRGE
HHHHCCCCCCCCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHEEEECCCCCHHHCCC
KGQNRHLGHIPRAVNMEWTEAMDRQRDLRIRDYAELITELDALGLTPDMEIVTHCQSHHR
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
SGFTWLVGKALGFEKMRAYPGSWQEWGNRDDTPIER
CCCEEEHHHHHCHHHHHCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSEDNLLPLIVEPEQLAEHLDAPDLLIVDVPLKAESYTTGHVPGAIFLDHRRLLAGDGP
CCCCCEEEEEECHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCEECCCCC
VPNDVPDEDALSALFSELGLTRDTHVVAYDDEGGGWAGRLLWTLELIGHTRYSYLNGGIH
CCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
AWRAAGLTQSREPHQPQPSDYRAEIRNPGVMIERDELLQRLDEVQLAVWDARSPAEYRGE
HHHHCCCCCCCCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHEEEECCCCCHHHCCC
KGQNRHLGHIPRAVNMEWTEAMDRQRDLRIRDYAELITELDALGLTPDMEIVTHCQSHHR
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
SGFTWLVGKALGFEKMRAYPGSWQEWGNRDDTPIER
CCCEEEHHHHHCHHHHHCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]