Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 92114617

GI number: 92114617

Start: 2807517

End: 2809502

Strand: Direct

Name: slt [H]

Synonym: Csal_2499

Alternate gene names: 92114617

Gene position: 2807517-2809502 (Clockwise)

Preceding gene: 92114611

Following gene: 92114621

Centisome position: 75.95

GC content: 67.88

Gene sequence:

>1986_bases
ATGCTCGCATCGCGACTTCGTCATCGCTGGATGGCGCTGTTCTCGGCGGCCACGCTGAGTCTGGCGCTGCACGCCTTCGA
CGCGCAGGCACAAGCCGACACGGACACGCTAGCGCCCGAAGACCGGGCCATGAAACGCGCCCTCGATGCCGCACGCGACC
ATCAATGGTCGCGCATCGAGAGCGTTGCCGTCGACGACCACGTGCTCGCCGGCTATGTGGATTATCATCGCCTCAAATCG
CGCCTCCCGAACGTCACGGCCGAAGAGGTGCAGAGCTATGTTTCGCGGCACGCCGACTCGCCCCTTTCCGGCTGGATGCG
AGGGCAGGCGCAGATGGCCTTCGGTCGTGACGGGCGCTTCGCCGACCTGCTGGCCATCAGCGACGGCGAGCCCCGAGGGA
CCATTCGCCAGTGCTATTACTACACGGCCCTGCTCGACCGCGCCCCCGACGAAGCCGCAGAGGGCGGACGCGCCCTGTGG
CGGGTCGGCCGCTCCCAGCCCAGCGCCTGTGACCGGCTGTTCGACACCCTGCGCGAACGCGGCGCCATCGACGACAGCGA
CATCTGGACACGCCTGACACTCGCCTGGCAGAACGGCGAAAGTCGCCTGGTCGATTATCTCGACGGCCTGTTGGGGCCCC
AGTGGCAAGCCGGCGTCGAGGCACTCGATCGCCTGCGCGGCAACTACGCCGCCATCACCGACGTACCCACTCGCATCGGC
CCGTCGGGGGACGGCGCCGGCGCCCTGTACGCCGCGGCCATGCACAACTTCACGCGTGCCGATACCGAAGCCGCGCTCGA
GGCCTGGCGCAAGATCGGCCCGCACCTCACGCTCAGCGAGGCCCGACGCCACGCGATAGAACATGACCTGGCGTTTTACT
CGATGGTCCGCGAGATCGACAACAATGCCGGCTGGGTGGATGCCGCACTGCCCCGCCTGGCGGATGCCGACCTGTTCGAG
TTGCGTGTTCGCAATGCGTTGGCCTCGCAACGCTGGGGCGAAGTCATCGAGTGGATCGATGCCATGCCGCGGGACACGCG
GCAAGAGGCACACTGGCAATACTGGCTGGGGCGTGCGCTCGCGCAGCGCGGCGATCGCCAGGCGGCGGAAGACGCCTGGC
GACTCGCCGCCCAGGAACGCAGCTTTTTCGGTTTCGCCGCCGCCGACAGGCTCGGCCAGCCCTACGCCCTGAACATGGAC
ATGCCCGAGGTGTCGCCGCTGAAGAGCGAGCTGGCCGCCATGCAGCCCGTCGTGCAGCGCAGCGAAGCCTTGCGTCGCAT
CGGCGAGCCCGGCCTGGCGCGCAGCGAGTGGTTCCATGCCCTCGGCGAGGCGAACGACGCCGATGCCGCTGCGCTCAACG
CCTACGCGCTCGAGCGCGGGTGGTATGACCTGGCCGTGCACGGCACGATCATGGCCAAGCAGTGGGATGCACTCGCCTGG
CGCTTTCCACCCGCCTACCGGGACGCCTTCAGCGCCTGGGGGCAGCGCAATGGAGTCGATCCCTACCTGCTGATGGCGAT
CTCCCGTCGCGAGAGCGCGTTCAACCGCGAGGCGGTATCCCCCGTCGGCGCACGTGGGCTCATGCAGCTGATGCCGGGCA
CCGCGGACCATGTCAGCCGCGACCTGAACATCGACGCGCCCACGCTGGGCGAACTGTTCGAGCCCACCACCAATATCCGG
CTGGGCAGCGCCTACATCCGCGACATGATGGAGCGCTACCGTGGCAACCGCATCGCGGCGGCGGCGGCCTACAACGCCGG
CCCCGGTCGGGTCGACCGCTGGTTGCGCGACTCGCCCGATGCGTTCGACCTCTTCGTCGAAAGCATCCCCTTCCGCGAGA
CCCGCGCTTACGTGCAGGCCGTGCTGACCTATCGGGTCATTTTCGAAAGTCTTGCCAAGCAAGGCGATACGCATGGAGTC
GCGCTCTTGACCCAGTCGGAGCGCGAAGGCGCCTACGACACCACACTGCTGGCACGCAACCCATGA

Upstream 100 bases:

>100_bases
CGCGCAGGGCGTCGCCGCCTGGCAACACTACCCAAGACACCGTCCCTGAGCGAGGATGATCACATCATCATAACGCCGGG
CAAGCAAGGAGACATGGAAG

Downstream 100 bases:

>100_bases
CGAAAGCGCCGACGCGTCATGCGACGCGTCGGCGCTGATGACCAGGCCCTGCCGGCGTCAGGGTCGCTGCGCCAACGCCA
GCTTCACCCCGAACAGCACC

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MLASRLRHRWMALFSAATLSLALHAFDAQAQADTDTLAPEDRAMKRALDAARDHQWSRIESVAVDDHVLAGYVDYHRLKS
RLPNVTAEEVQSYVSRHADSPLSGWMRGQAQMAFGRDGRFADLLAISDGEPRGTIRQCYYYTALLDRAPDEAAEGGRALW
RVGRSQPSACDRLFDTLRERGAIDDSDIWTRLTLAWQNGESRLVDYLDGLLGPQWQAGVEALDRLRGNYAAITDVPTRIG
PSGDGAGALYAAAMHNFTRADTEAALEAWRKIGPHLTLSEARRHAIEHDLAFYSMVREIDNNAGWVDAALPRLADADLFE
LRVRNALASQRWGEVIEWIDAMPRDTRQEAHWQYWLGRALAQRGDRQAAEDAWRLAAQERSFFGFAAADRLGQPYALNMD
MPEVSPLKSELAAMQPVVQRSEALRRIGEPGLARSEWFHALGEANDADAAALNAYALERGWYDLAVHGTIMAKQWDALAW
RFPPAYRDAFSAWGQRNGVDPYLLMAISRRESAFNREAVSPVGARGLMQLMPGTADHVSRDLNIDAPTLGELFEPTTNIR
LGSAYIRDMMERYRGNRIAAAAAYNAGPGRVDRWLRDSPDAFDLFVESIPFRETRAYVQAVLTYRVIFESLAKQGDTHGV
ALLTQSEREGAYDTTLLARNP

Sequences:

>Translated_661_residues
MLASRLRHRWMALFSAATLSLALHAFDAQAQADTDTLAPEDRAMKRALDAARDHQWSRIESVAVDDHVLAGYVDYHRLKS
RLPNVTAEEVQSYVSRHADSPLSGWMRGQAQMAFGRDGRFADLLAISDGEPRGTIRQCYYYTALLDRAPDEAAEGGRALW
RVGRSQPSACDRLFDTLRERGAIDDSDIWTRLTLAWQNGESRLVDYLDGLLGPQWQAGVEALDRLRGNYAAITDVPTRIG
PSGDGAGALYAAAMHNFTRADTEAALEAWRKIGPHLTLSEARRHAIEHDLAFYSMVREIDNNAGWVDAALPRLADADLFE
LRVRNALASQRWGEVIEWIDAMPRDTRQEAHWQYWLGRALAQRGDRQAAEDAWRLAAQERSFFGFAAADRLGQPYALNMD
MPEVSPLKSELAAMQPVVQRSEALRRIGEPGLARSEWFHALGEANDADAAALNAYALERGWYDLAVHGTIMAKQWDALAW
RFPPAYRDAFSAWGQRNGVDPYLLMAISRRESAFNREAVSPVGARGLMQLMPGTADHVSRDLNIDAPTLGELFEPTTNIR
LGSAYIRDMMERYRGNRIAAAAAYNAGPGRVDRWLRDSPDAFDLFVESIPFRETRAYVQAVLTYRVIFESLAKQGDTHGV
ALLTQSEREGAYDTTLLARNP
>Mature_661_residues
MLASRLRHRWMALFSAATLSLALHAFDAQAQADTDTLAPEDRAMKRALDAARDHQWSRIESVAVDDHVLAGYVDYHRLKS
RLPNVTAEEVQSYVSRHADSPLSGWMRGQAQMAFGRDGRFADLLAISDGEPRGTIRQCYYYTALLDRAPDEAAEGGRALW
RVGRSQPSACDRLFDTLRERGAIDDSDIWTRLTLAWQNGESRLVDYLDGLLGPQWQAGVEALDRLRGNYAAITDVPTRIG
PSGDGAGALYAAAMHNFTRADTEAALEAWRKIGPHLTLSEARRHAIEHDLAFYSMVREIDNNAGWVDAALPRLADADLFE
LRVRNALASQRWGEVIEWIDAMPRDTRQEAHWQYWLGRALAQRGDRQAAEDAWRLAAQERSFFGFAAADRLGQPYALNMD
MPEVSPLKSELAAMQPVVQRSEALRRIGEPGLARSEWFHALGEANDADAAALNAYALERGWYDLAVHGTIMAKQWDALAW
RFPPAYRDAFSAWGQRNGVDPYLLMAISRRESAFNREAVSPVGARGLMQLMPGTADHVSRDLNIDAPTLGELFEPTTNIR
LGSAYIRDMMERYRGNRIAAAAAYNAGPGRVDRWLRDSPDAFDLFVESIPFRETRAYVQAVLTYRVIFESLAKQGDTHGV
ALLTQSEREGAYDTTLLARNP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=567, Percent_Identity=33.6860670194003, Blast_Score=285, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73922; Mature: 73922

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASRLRHRWMALFSAATLSLALHAFDAQAQADTDTLAPEDRAMKRALDAARDHQWSRIE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
SVAVDDHVLAGYVDYHRLKSRLPNVTAEEVQSYVSRHADSPLSGWMRGQAQMAFGRDGRF
HHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHEEECCCCCE
ADLLAISDGEPRGTIRQCYYYTALLDRAPDEAAEGGRALWRVGRSQPSACDRLFDTLRER
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHCCCCCHHHHHHHHHHHHC
GAIDDSDIWTRLTLAWQNGESRLVDYLDGLLGPQWQAGVEALDRLRGNYAAITDVPTRIG
CCCCCCHHHEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCHHCC
PSGDGAGALYAAAMHNFTRADTEAALEAWRKIGPHLTLSEARRHAIEHDLAFYSMVREID
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NNAGWVDAALPRLADADLFELRVRNALASQRWGEVIEWIDAMPRDTRQEAHWQYWLGRAL
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AQRGDRQAAEDAWRLAAQERSFFGFAAADRLGQPYALNMDMPEVSPLKSELAAMQPVVQR
HHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHH
SEALRRIGEPGLARSEWFHALGEANDADAAALNAYALERGWYDLAVHGTIMAKQWDALAW
HHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHC
RFPPAYRDAFSAWGQRNGVDPYLLMAISRRESAFNREAVSPVGARGLMQLMPGTADHVSR
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCC
DLNIDAPTLGELFEPTTNIRLGSAYIRDMMERYRGNRIAAAAAYNAGPGRVDRWLRDSPD
CCCCCCCCHHHHHCCCCCEEECHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCC
AFDLFVESIPFRETRAYVQAVLTYRVIFESLAKQGDTHGVALLTQSEREGAYDTTLLARN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECC
P
C
>Mature Secondary Structure
MLASRLRHRWMALFSAATLSLALHAFDAQAQADTDTLAPEDRAMKRALDAARDHQWSRIE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
SVAVDDHVLAGYVDYHRLKSRLPNVTAEEVQSYVSRHADSPLSGWMRGQAQMAFGRDGRF
HHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHEEECCCCCE
ADLLAISDGEPRGTIRQCYYYTALLDRAPDEAAEGGRALWRVGRSQPSACDRLFDTLRER
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHCCCCCHHHHHHHHHHHHC
GAIDDSDIWTRLTLAWQNGESRLVDYLDGLLGPQWQAGVEALDRLRGNYAAITDVPTRIG
CCCCCCHHHEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCHHCC
PSGDGAGALYAAAMHNFTRADTEAALEAWRKIGPHLTLSEARRHAIEHDLAFYSMVREID
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NNAGWVDAALPRLADADLFELRVRNALASQRWGEVIEWIDAMPRDTRQEAHWQYWLGRAL
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AQRGDRQAAEDAWRLAAQERSFFGFAAADRLGQPYALNMDMPEVSPLKSELAAMQPVVQR
HHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHH
SEALRRIGEPGLARSEWFHALGEANDADAAALNAYALERGWYDLAVHGTIMAKQWDALAW
HHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHC
RFPPAYRDAFSAWGQRNGVDPYLLMAISRRESAFNREAVSPVGARGLMQLMPGTADHVSR
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCC
DLNIDAPTLGELFEPTTNIRLGSAYIRDMMERYRGNRIAAAAAYNAGPGRVDRWLRDSPD
CCCCCCCCHHHHHCCCCCEEECHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCC
AFDLFVESIPFRETRAYVQAVLTYRVIFESLAKQGDTHGVALLTQSEREGAYDTTLLARN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]