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The map label for this gene is pmpM [H]

Identifier: 92114521

GI number: 92114521

Start: 2693386

End: 2694813

Strand: Direct

Name: pmpM [H]

Synonym: Csal_2403

Alternate gene names: 92114521

Gene position: 2693386-2694813 (Clockwise)

Preceding gene: 92114518

Following gene: 92114522

Centisome position: 72.86

GC content: 67.09

Gene sequence:

>1428_bases
GTGACGTCGCCCCGAGCCCTGCTCACCCATAGCCACCGTGAATCGCGCACCTTGCTGCGCCTGGCGTTGCCGATCTGCGG
CGCACAGCTGGCGCAATCGGGCATGAGCGCGGTCGATGTGATCATGGCCGGTCGCGCCAGTGCCACGGACCTGGCCGCCG
TCTCGGTCGGCTCCAGCCTGTGGCTGCCGTTGATGCTCTTCATGACCGGCGCGCTGATGGGCCTGACGCCCATCGTGGCG
CAATTGCTGGGCGCGGGGCGCCACACGGCGATTCGGCCCTATGTTCACCAGGCGCTGTGGATCGCGCTGTGCCTGGGCGG
GCTGTCGGCGCTCTTGCTATGGTTCGGCGTGGGCCCGGTCTTCCGATTCATGGGCGTGCCGGAGCAGGTCGCGCAACTCT
CCGCCGGCTACCTGGCCGCCGTGGCGTTCGGCATGCCAGGCGTGGGCCTTTACCAGGCCTTGCGCGCCTTCTCCGACGGC
ATGAACCACACGCGCCCCGCCCTGTGGATCAGCCTGCTGGGGCTGGCGATCAATATTCCCTGCAACTATGTGCTGATCTA
CGGCGGGCCCGGCCTGGTCTCGTTGCTGGGCGAGACGGTACCGCCATGGATCGCCGCCTGGCCGGCGCTGGGCGCGGTGG
GATGCGGCGTGGCCACGGCGATTTCGATGTGGGTGATGTGCCTGGCGATGATCGGCTACACGTACCGGGCACGCGCCTAC
GGCGCGGTGCGCTTGTGGCACTCGCCGGTTCCGCCGCAACGCCGCGACATCCAGGAGCTGCTTTACGTCGGGGTGCCCAT
CGGCGTGGCGATCTTCGTCGAGGTCACGCTGTTCACCCTGATCGCCCTGTTCGTCGCGCGCCTGGGGGAAGTGGTCGTCG
CCGCCCACCAGATCGCACTCAACTATACGTCGATCCTGTTCATGCTGCCGTTGTCGCTGAGCATGGCGCTGACCGTGCGC
GTGGGCAATACGCTGGGACGCGGAACCGCACAGGAGGCACGCTTCGTGGCCTGGAACGGCGTCGTCGTGTGTCTGCTGGT
GGCCCTGCTCAACGACGCCATTCTCTGGATGACGGCGTCGCCCATCCTGGCGCTGTATACGTCGAACACGGAAGTGCATG
CTCTGGCCATGTCGCTGGTCACGCTGGCCATGCTCTATCAGATCTCCGATTCGCTGCAGGTCAATCTCGCCGGCGCGCTG
CGCGGTTACAAGGACACGCGCATCGTCATGCTGATCACGCTGTTTTCCTATTGGCTGATCGGACTGGCCGGGGGCCACCT
GCTGGGACGTGGCATCGACGGGTACCTGGCGCCGATGGGCGTGCACGGCTATTGGCTCGGCCTGTGCGCGGGGCTGACGG
TCGCCGCGCTGTTACTCGGCTGGCGCCTGCATCATCGGGCGCGTCGTAGCCTGAGCGCCTACGCTTGA

Upstream 100 bases:

>100_bases
ATGAAAGGCGTCGACGTGGCACGCTTGCTAGAATGCCCGCTTTTGCGCCCGTGAAAGTTCTGAAGCGAGTCCGTGAAGAA
AGTCCACGAGGAGAGTCAGT

Downstream 100 bases:

>100_bases
CATTCAGCCTTGCGGTGACGTGTCGCTCATCAGGCTCGGTGGGACATCCTTCAAGCGCAATAACGTTGATCTCACCAACG
CCAACAGACCGTTTTAATGT

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: H(+)/drug antiporter; Multidrug-efflux transporter [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTSPRALLTHSHRESRTLLRLALPICGAQLAQSGMSAVDVIMAGRASATDLAAVSVGSSLWLPLMLFMTGALMGLTPIVA
QLLGAGRHTAIRPYVHQALWIALCLGGLSALLLWFGVGPVFRFMGVPEQVAQLSAGYLAAVAFGMPGVGLYQALRAFSDG
MNHTRPALWISLLGLAINIPCNYVLIYGGPGLVSLLGETVPPWIAAWPALGAVGCGVATAISMWVMCLAMIGYTYRARAY
GAVRLWHSPVPPQRRDIQELLYVGVPIGVAIFVEVTLFTLIALFVARLGEVVVAAHQIALNYTSILFMLPLSLSMALTVR
VGNTLGRGTAQEARFVAWNGVVVCLLVALLNDAILWMTASPILALYTSNTEVHALAMSLVTLAMLYQISDSLQVNLAGAL
RGYKDTRIVMLITLFSYWLIGLAGGHLLGRGIDGYLAPMGVHGYWLGLCAGLTVAALLLGWRLHHRARRSLSAYA

Sequences:

>Translated_475_residues
MTSPRALLTHSHRESRTLLRLALPICGAQLAQSGMSAVDVIMAGRASATDLAAVSVGSSLWLPLMLFMTGALMGLTPIVA
QLLGAGRHTAIRPYVHQALWIALCLGGLSALLLWFGVGPVFRFMGVPEQVAQLSAGYLAAVAFGMPGVGLYQALRAFSDG
MNHTRPALWISLLGLAINIPCNYVLIYGGPGLVSLLGETVPPWIAAWPALGAVGCGVATAISMWVMCLAMIGYTYRARAY
GAVRLWHSPVPPQRRDIQELLYVGVPIGVAIFVEVTLFTLIALFVARLGEVVVAAHQIALNYTSILFMLPLSLSMALTVR
VGNTLGRGTAQEARFVAWNGVVVCLLVALLNDAILWMTASPILALYTSNTEVHALAMSLVTLAMLYQISDSLQVNLAGAL
RGYKDTRIVMLITLFSYWLIGLAGGHLLGRGIDGYLAPMGVHGYWLGLCAGLTVAALLLGWRLHHRARRSLSAYA
>Mature_474_residues
TSPRALLTHSHRESRTLLRLALPICGAQLAQSGMSAVDVIMAGRASATDLAAVSVGSSLWLPLMLFMTGALMGLTPIVAQ
LLGAGRHTAIRPYVHQALWIALCLGGLSALLLWFGVGPVFRFMGVPEQVAQLSAGYLAAVAFGMPGVGLYQALRAFSDGM
NHTRPALWISLLGLAINIPCNYVLIYGGPGLVSLLGETVPPWIAAWPALGAVGCGVATAISMWVMCLAMIGYTYRARAYG
AVRLWHSPVPPQRRDIQELLYVGVPIGVAIFVEVTLFTLIALFVARLGEVVVAAHQIALNYTSILFMLPLSLSMALTVRV
GNTLGRGTAQEARFVAWNGVVVCLLVALLNDAILWMTASPILALYTSNTEVHALAMSLVTLAMLYQISDSLQVNLAGALR
GYKDTRIVMLITLFSYWLIGLAGGHLLGRGIDGYLAPMGVHGYWLGLCAGLTVAALLLGWRLHHRARRSLSAYA

Specific function: Multidrug efflux pump that functions as an H(+)/drug antiporter. Confers resistance to benzalkonium chloride, fluoroquinolones, ethidium bromide, acriflavine and tetraphenylphosphonium chloride [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=351, Percent_Identity=22.5071225071225, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI48994890, Length=456, Percent_Identity=42.9824561403509, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI226510954, Length=478, Percent_Identity=25.5230125523013, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321821, Length=407, Percent_Identity=19.6560196560197, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320545, Length=468, Percent_Identity=20.2991452991453, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 50754; Mature: 50623

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPRALLTHSHRESRTLLRLALPICGAQLAQSGMSAVDVIMAGRASATDLAAVSVGSSL
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCCCHH
WLPLMLFMTGALMGLTPIVAQLLGAGRHTAIRPYVHQALWIALCLGGLSALLLWFGVGPV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRFMGVPEQVAQLSAGYLAAVAFGMPGVGLYQALRAFSDGMNHTRPALWISLLGLAINIP
HHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECC
CNYVLIYGGPGLVSLLGETVPPWIAAWPALGAVGCGVATAISMWVMCLAMIGYTYRARAY
CCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GAVRLWHSPVPPQRRDIQELLYVGVPIGVAIFVEVTLFTLIALFVARLGEVVVAAHQIAL
HEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYTSILFMLPLSLSMALTVRVGNTLGRGTAQEARFVAWNGVVVCLLVALLNDAILWMTAS
HHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHCCEEEHHHHHHHHHHHHHCCHHEEEECC
PILALYTSNTEVHALAMSLVTLAMLYQISDSLQVNLAGALRGYKDTRIVMLITLFSYWLI
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHH
GLAGGHLLGRGIDGYLAPMGVHGYWLGLCAGLTVAALLLGWRLHHRARRSLSAYA
HHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSPRALLTHSHRESRTLLRLALPICGAQLAQSGMSAVDVIMAGRASATDLAAVSVGSSL
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCCCHH
WLPLMLFMTGALMGLTPIVAQLLGAGRHTAIRPYVHQALWIALCLGGLSALLLWFGVGPV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRFMGVPEQVAQLSAGYLAAVAFGMPGVGLYQALRAFSDGMNHTRPALWISLLGLAINIP
HHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECC
CNYVLIYGGPGLVSLLGETVPPWIAAWPALGAVGCGVATAISMWVMCLAMIGYTYRARAY
CCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GAVRLWHSPVPPQRRDIQELLYVGVPIGVAIFVEVTLFTLIALFVARLGEVVVAAHQIAL
HEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYTSILFMLPLSLSMALTVRVGNTLGRGTAQEARFVAWNGVVVCLLVALLNDAILWMTAS
HHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHCCEEEHHHHHHHHHHHHHCCHHEEEECC
PILALYTSNTEVHALAMSLVTLAMLYQISDSLQVNLAGALRGYKDTRIVMLITLFSYWLI
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHH
GLAGGHLLGRGIDGYLAPMGVHGYWLGLCAGLTVAALLLGWRLHHRARRSLSAYA
HHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]