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The map label for this gene is proS

Identifier: 92114261

GI number: 92114261

Start: 2406840

End: 2408561

Strand: Direct

Name: proS

Synonym: Csal_2139

Alternate gene names: 92114261

Gene position: 2406840-2408561 (Clockwise)

Preceding gene: 92114260

Following gene: 92114262

Centisome position: 65.11

GC content: 64.98

Gene sequence:

>1722_bases
ATGCGCGCCAGTCAATTGCTGATCTCCACCCTCAAGGAAACCCCTGCCGACGCCGATATCGTCAGCCATCAGCTGATGCT
GCGCGCGGGCATGATCCGCCGCCTGAGCTCGGGGCTCTATACCTGGCTCCCCCTGGGACTTCGCACCCTTCGCAAGGTCG
AGAACATCGTGCGCGAGGAAATGAATCGCGCCGGCGCCCAAGAAGTGCTGATGCCGTCGATCCAGCCGGCGGAACTGTGG
CAGGAGTCCGGACGCTGGGATCAGTACGGCAACCTGTTGTTGCGCATTCGCGACCGCCACGAGCGCGATTTCTGCTATGG
TCCGACGCACGAGGAAGTGATCACCGACCTGGTGCGCAACGAAATACGCTCGTACAAGCAGTTGCCCTCCAACTTCTACC
AGATCCAGACCAAGTTCCGCGACGAGACGCGCCCGCGTTTCGGTGTCATGCGCGCCCGCGAGTTCATCATGAAGGATGCC
TACTCCTTCGATATCGACCAGGCCGGCCTGCAACGCTCCTACGATGCCATGTACGACGCCTACATGCGCATCTTCACGCG
CCTGGGGCTGGATTTCCGCGCCGTCGAGGCAGACAACGGCGACATCGGCGGCAGCGGCTCCCACGAATTCCAGGTGCTGG
CCGACTCCGGCGAGGACGCGGTCATCTTCTCCACCGGCTCCGACTACGCTGCCAACATCGAGAAGGCCGAGGCACTGCCC
GCGCCCTTGGGCGAGACACCTGAACGCCCCGCTCCTCAGGAAGAACTGCGACTGGTCGACACGCCGAATGCGCGCACCAT
CGCCACCCTGGTCGAGCAGCACGGCCTGCCCATCGAGAAGACCATCAAGACGCTGATGGTGCACGGCAGCGAGGGTGGAC
TGGTCGCCCTGCTGGTGCGCGGCGATCACGAGCTCAACGAGGTCAAGGCCGAGAACCTCCCCGAGGTCGCCGCTCCCCTG
ACCATGGCCAGCGAGGAGGAAATTCGCGCGGCGGTGGGCGCCGGCCCCGGCTCACTCGGCCCGGTGAACCTCGATATGCC
GCTGATCATCGACCGCAGCGTGGCGCTGATGAGCGACTTCGGCGCCGGCGCGAACATCGACGGCCAGCACTACTTCGGCA
TCAACTGGGAGCGCGACGTCGCCCTGCCCAAGGTGGCCGACCTGCGCAACGTGGTCGAGGGCGATCCCTCGCCGGACGGC
AAGGGCACGCTCTCCATCGCCCGCGGCATCGAGGTCGGCCACGTCTTCCAGCTGGGCACCAAGTATTCCACGGCGATGAA
TGCGACCGTGCTCGACGACAACGGCCAGGCCGTGCCCCTGCTGATGGGCTGTTACGGCATCGGCGTGACCCGCGTGGTCG
CCGCCGCCATCGAGCAGAATCACGACGCCGGCGGGATCATCTGGCCGGATGCCATCGCCCCGTTCGAGATCGCTCTGGTG
CCCATGAACGCCCACAAGTCGGAACGGGTCCGCGAATACGCCGACACCTTGTATCAGCAGCTCAGCGATGCCGGCTTCGA
TGTCCTGATCGACGACCGCGACCTGCGGCCCGGCGTCAAGTTCGCCGACCAGGAGCTCATCGGCATTCCGCACCGCGTCG
TGATCGGCGATCGCGGCCTCGATAACGATGAGCTGGAATACAAGGGACGGCGCGACAGCGACGTCACCATGGTTCCCACG
GAAGGGCTGCTGAGCTTTCTGCGTGAACGCATCACGAAGTGA

Upstream 100 bases:

>100_bases
CTTTTGCCGCGTTAACGCCTTGACCGTACAATAGCCTCATCTGCTGCGCCGCTTTCGAGCGACGCGATGTTAGGCCTCGA
ATCCAAGGAACCGCTTGTCC

Downstream 100 bases:

>100_bases
AGCCTCCGCGCGTTGACGATCGTCGGGGTGCACAACCGCACCCCGACCGTGCTTCCCTCCACCCCTGATTCACTGACAAG
ATGGATCTGCGGCGGGCGAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MRASQLLISTLKETPADADIVSHQLMLRAGMIRRLSSGLYTWLPLGLRTLRKVENIVREEMNRAGAQEVLMPSIQPAELW
QESGRWDQYGNLLLRIRDRHERDFCYGPTHEEVITDLVRNEIRSYKQLPSNFYQIQTKFRDETRPRFGVMRAREFIMKDA
YSFDIDQAGLQRSYDAMYDAYMRIFTRLGLDFRAVEADNGDIGGSGSHEFQVLADSGEDAVIFSTGSDYAANIEKAEALP
APLGETPERPAPQEELRLVDTPNARTIATLVEQHGLPIEKTIKTLMVHGSEGGLVALLVRGDHELNEVKAENLPEVAAPL
TMASEEEIRAAVGAGPGSLGPVNLDMPLIIDRSVALMSDFGAGANIDGQHYFGINWERDVALPKVADLRNVVEGDPSPDG
KGTLSIARGIEVGHVFQLGTKYSTAMNATVLDDNGQAVPLLMGCYGIGVTRVVAAAIEQNHDAGGIIWPDAIAPFEIALV
PMNAHKSERVREYADTLYQQLSDAGFDVLIDDRDLRPGVKFADQELIGIPHRVVIGDRGLDNDELEYKGRRDSDVTMVPT
EGLLSFLRERITK

Sequences:

>Translated_573_residues
MRASQLLISTLKETPADADIVSHQLMLRAGMIRRLSSGLYTWLPLGLRTLRKVENIVREEMNRAGAQEVLMPSIQPAELW
QESGRWDQYGNLLLRIRDRHERDFCYGPTHEEVITDLVRNEIRSYKQLPSNFYQIQTKFRDETRPRFGVMRAREFIMKDA
YSFDIDQAGLQRSYDAMYDAYMRIFTRLGLDFRAVEADNGDIGGSGSHEFQVLADSGEDAVIFSTGSDYAANIEKAEALP
APLGETPERPAPQEELRLVDTPNARTIATLVEQHGLPIEKTIKTLMVHGSEGGLVALLVRGDHELNEVKAENLPEVAAPL
TMASEEEIRAAVGAGPGSLGPVNLDMPLIIDRSVALMSDFGAGANIDGQHYFGINWERDVALPKVADLRNVVEGDPSPDG
KGTLSIARGIEVGHVFQLGTKYSTAMNATVLDDNGQAVPLLMGCYGIGVTRVVAAAIEQNHDAGGIIWPDAIAPFEIALV
PMNAHKSERVREYADTLYQQLSDAGFDVLIDDRDLRPGVKFADQELIGIPHRVVIGDRGLDNDELEYKGRRDSDVTMVPT
EGLLSFLRERITK
>Mature_573_residues
MRASQLLISTLKETPADADIVSHQLMLRAGMIRRLSSGLYTWLPLGLRTLRKVENIVREEMNRAGAQEVLMPSIQPAELW
QESGRWDQYGNLLLRIRDRHERDFCYGPTHEEVITDLVRNEIRSYKQLPSNFYQIQTKFRDETRPRFGVMRAREFIMKDA
YSFDIDQAGLQRSYDAMYDAYMRIFTRLGLDFRAVEADNGDIGGSGSHEFQVLADSGEDAVIFSTGSDYAANIEKAEALP
APLGETPERPAPQEELRLVDTPNARTIATLVEQHGLPIEKTIKTLMVHGSEGGLVALLVRGDHELNEVKAENLPEVAAPL
TMASEEEIRAAVGAGPGSLGPVNLDMPLIIDRSVALMSDFGAGANIDGQHYFGINWERDVALPKVADLRNVVEGDPSPDG
KGTLSIARGIEVGHVFQLGTKYSTAMNATVLDDNGQAVPLLMGCYGIGVTRVVAAAIEQNHDAGGIIWPDAIAPFEIALV
PMNAHKSERVREYADTLYQQLSDAGFDVLIDDRDLRPGVKFADQELIGIPHRVVIGDRGLDNDELEYKGRRDSDVTMVPT
EGLLSFLRERITK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=46.9298245614035, Blast_Score=227, Evalue=2e-59,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=69.8606271777004, Blast_Score=828, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=208, Percent_Identity=38.9423076923077, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=31.7647058823529, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=226, Percent_Identity=39.3805309734513, Blast_Score=180, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24656200, Length=218, Percent_Identity=37.6146788990826, Blast_Score=168, Evalue=7e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_CHRSD (Q1QVL8)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574189.1
- ProteinModelPortal:   Q1QVL8
- SMR:   Q1QVL8
- STRING:   Q1QVL8
- GeneID:   4026479
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2139
- NMPDR:   fig|290398.4.peg.2385
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- BioCyc:   CSAL290398:CSAL_2139-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63437; Mature: 63437

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASQLLISTLKETPADADIVSHQLMLRAGMIRRLSSGLYTWLPLGLRTLRKVENIVREE
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MNRAGAQEVLMPSIQPAELWQESGRWDQYGNLLLRIRDRHERDFCYGPTHEEVITDLVRN
HHHCCCHHHHCCCCCHHHHHHHCCCCCHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
EIRSYKQLPSNFYQIQTKFRDETRPRFGVMRAREFIMKDAYSFDIDQAGLQRSYDAMYDA
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YMRIFTRLGLDFRAVEADNGDIGGSGSHEFQVLADSGEDAVIFSTGSDYAANIEKAEALP
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHCCHHHHCCC
APLGETPERPAPQEELRLVDTPNARTIATLVEQHGLPIEKTIKTLMVHGSEGGLVALLVR
CCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHCCCCHHHHHHHHEEECCCCCEEEEEEE
GDHELNEVKAENLPEVAAPLTMASEEEIRAAVGAGPGSLGPVNLDMPLIIDRSVALMSDF
CCCHHHHHHHCCCCHHHHCCHHCCHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHC
GAGANIDGQHYFGINWERDVALPKVADLRNVVEGDPSPDGKGTLSIARGIEVGHVFQLGT
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEHHCCCCCCHHEECCC
KYSTAMNATVLDDNGQAVPLLMGCYGIGVTRVVAAAIEQNHDAGGIIWPDAIAPFEIALV
CHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEE
PMNAHKSERVREYADTLYQQLSDAGFDVLIDDRDLRPGVKFADQELIGIPHRVVIGDRGL
ECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCCEEEEECCCC
DNDELEYKGRRDSDVTMVPTEGLLSFLRERITK
CCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MRASQLLISTLKETPADADIVSHQLMLRAGMIRRLSSGLYTWLPLGLRTLRKVENIVREE
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MNRAGAQEVLMPSIQPAELWQESGRWDQYGNLLLRIRDRHERDFCYGPTHEEVITDLVRN
HHHCCCHHHHCCCCCHHHHHHHCCCCCHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
EIRSYKQLPSNFYQIQTKFRDETRPRFGVMRAREFIMKDAYSFDIDQAGLQRSYDAMYDA
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YMRIFTRLGLDFRAVEADNGDIGGSGSHEFQVLADSGEDAVIFSTGSDYAANIEKAEALP
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHCCHHHHCCC
APLGETPERPAPQEELRLVDTPNARTIATLVEQHGLPIEKTIKTLMVHGSEGGLVALLVR
CCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHCCCCHHHHHHHHEEECCCCCEEEEEEE
GDHELNEVKAENLPEVAAPLTMASEEEIRAAVGAGPGSLGPVNLDMPLIIDRSVALMSDF
CCCHHHHHHHCCCCHHHHCCHHCCHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHC
GAGANIDGQHYFGINWERDVALPKVADLRNVVEGDPSPDGKGTLSIARGIEVGHVFQLGT
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEHHCCCCCCHHEECCC
KYSTAMNATVLDDNGQAVPLLMGCYGIGVTRVVAAAIEQNHDAGGIIWPDAIAPFEIALV
CHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEE
PMNAHKSERVREYADTLYQQLSDAGFDVLIDDRDLRPGVKFADQELIGIPHRVVIGDRGL
ECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCCEEEEECCCC
DNDELEYKGRRDSDVTMVPTEGLLSFLRERITK
CCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA