Click here to switch to the map view.

The map label for this gene is rlmL

Identifier: 92113470

GI number: 92113470

Start: 1540489

End: 1542687

Strand: Direct

Name: rlmL

Synonym: Csal_1344

Alternate gene names: 92113470

Gene position: 1540489-1542687 (Clockwise)

Preceding gene: 92113468

Following gene: 92113472

Centisome position: 41.67

GC content: 68.08

Gene sequence:

>2199_bases
ATGAACGACGTGCAATCCCAACCCTTGACGCTCTACATCGCCTGTCCGCGTGGGCTCGAGGGCTTGCTGGCCGATGAACT
CGCGGACTTCGGCGCGGAGGTGACCGGCACCACCGTGGCGGGCGTCCATGCTCAGGCCGATACGGCGATGGCGTATCGCA
TCTGCCTGTGGTCGCGCCTGGCGAATCGCGTGGTGCTATGTCTTCTGCGCGAGCAGGGCATCGAACGTCCCGAGGCCTTG
GTCGAGGCGGCGCGACGGGTGGACTGGGGCGCTCACCTGCGCGCCGGGAGCAGTCTGGCCGTGGATTTTCACGGTCAATC
CGAGCATATCCGTCATACCCGCTTCGGGGCGCAAAGCGTCAAGGATGGCATCGTCGATGCGATGCGCGCGGCCGGGCGCG
AGCGGCCGGCCGTCGATCTCAAGACGCCCGATGCCCGTATCTATGCACACCTGCATCGTGGGCGTCTGCTGCTGGGGCTC
GACCTGGTCGGAGGCAGCCTGCACCAGCGTGGCTACCGCCGTGACGCCGGCCACGCACCGCTCAAGGAAAACCTGGCCGC
GGCCCTGTTGATGCGCGCCGACTGGCCCGCTCGGGCGCGGCGGGGCGAGCCCCTGGTCGATCCGCTGTGCGGCGCGGGGA
CATTGCTCATCGAGGCGGCGCTGATGGCGGCGGACATTGCCCCCCAACTGTCGCGCGACTATTTCGCTTTCGAAGCCTGG
GTGGGGCACGACCCCGACGCGTGGCGGGAGCTCCAGCGCGAAGCGCAGGCGCGCGCCAACGTCGGGCGTCGTCGTGTGAA
GTCGAAGCTGTACGGGCGCGATCAGAGCCCGCCGGCGATCGCCGCCGCCAAGGCCAACGCCATGCGCGCGGGGATTCCCG
CCCTGATCGATCTGCAGGGCGCCGAGGTGGCGCAACTCACGCGGCCGGAAGGCGTCGACGGGCCGGGGCTGGTCATCACC
AACCCGCCGTACGGCGAGCGCCTGGGCGAGCTGCCCGAGGTGGTGCCCATCTACACCGCCCTGGGCGAGCGCCTGCGTGC
CGAGTTCGAGGGCTGGCAGCTGGCGCTGTTCACCGGCAACCCCGACCTGGGGCATCGCACGGGGCTGCGTGCCGAAAAGC
AGTATGCGTTCAAGAACGGGCCACTGGACTGCAAGCTGCTGCTGATCCCGGTCGTGGCGCAGCGTACCGAGCGCGAGACG
TCCTCGGAAGGCGACGAGCCGCAGGGAGCAAGTGGGGCGACGTCCCGCCCCGGCCCTCGCAACGACGGGGCCCAGATGTT
CGCCAACCGTCTGGAAAAGAACCGCAAGCGGTTGAAGAAATGGCTCAAGCAGTCCGGCGAGCGCTGCTATCGGCTCTATG
ACGCGGACATGCCGGAATACGCCCTGGCAGTGGATGTCTACGGCGACCGTGTCCACGTGCAGGAGTATGCGCCACCGCGC
TCCGTGGACCCGCGCCAGGCACAGCGTCGCCTGCTCGACGCTCTGGAAGTCATTCCGCAGGTCCTGGGCGTGCGTCCGGA
AAGCGTGCATTACAAGCAGCGCACCCGGCAGGCGGGCAAGGCGCAGTATGCCAAGCAGGATGCCAGCGGCGAGCGGTTCG
AGGTCAGCGAAGGCCCGGCGCGGCTATGGGTCAATCTGCGCGATTACCTGGATACCGGGCTGTTCCTGGATCATCGTCCG
GTGCGCCGGCGGTTGCGCGAAATGGCCGACGGCAAGCGCTTCCTCAACCTGTTCTGCTACACGGGCACGGCCACGGTGCA
GGCGGCCCTGGGTGGCGCGAGCGACAGCGTCAGCGTCGACCTGTCCAATACCTACCTGGCCTGGGCACGCGACAACTTCG
CCCTCAACCGCCTCGACCCGAGCCGGCATCGCGTCGTGCGCGACGACTGCCTACGGTGGCTGGAAACGGCGGGCAGCGAG
TTCGACTTGATCTTCATGGATCCGCCGACGTTCTCCAATTCCAAGAAGATGGACGCCGTGCTCGACGTGCAGCGCGACCA
TGCACGGCTCATCGAGCTGGCCATGGCGCGCTTGGCGCCGGGCGGCACGCTGGTGTTTTCCAACAACCAGCGGCGCTTCG
TGCTCGATGCCGCCGTCGGGGAACGCTTCGCGGTGGAGGACATCTCGGCACGCACGTTCGACCCGGACTTCTCGCGGCGT
CCGGACCTGCATCACTGCTTCCTGATTCGCCACCGCTGA

Upstream 100 bases:

>100_bases
GCGTTGGCGCGGATTTGTCGACCAGCGATTTGGCATAAGCCAAACGTTCCATGTAAACCACAGGAATATGACCGGTGCGT
GGCGCCGGGATGACGACTTT

Downstream 100 bases:

>100_bases
GCGGTGCCGGTAGCAGGGAGGTCGTGTGGTTCGGCGGCGCTCAGGCGCCGTCGAGCAGCGACAGCTGGCGCATGGCTTCC
TGGGCTTCGGGGCTCGAGTC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MNDVQSQPLTLYIACPRGLEGLLADELADFGAEVTGTTVAGVHAQADTAMAYRICLWSRLANRVVLCLLREQGIERPEAL
VEAARRVDWGAHLRAGSSLAVDFHGQSEHIRHTRFGAQSVKDGIVDAMRAAGRERPAVDLKTPDARIYAHLHRGRLLLGL
DLVGGSLHQRGYRRDAGHAPLKENLAAALLMRADWPARARRGEPLVDPLCGAGTLLIEAALMAADIAPQLSRDYFAFEAW
VGHDPDAWRELQREAQARANVGRRRVKSKLYGRDQSPPAIAAAKANAMRAGIPALIDLQGAEVAQLTRPEGVDGPGLVIT
NPPYGERLGELPEVVPIYTALGERLRAEFEGWQLALFTGNPDLGHRTGLRAEKQYAFKNGPLDCKLLLIPVVAQRTERET
SSEGDEPQGASGATSRPGPRNDGAQMFANRLEKNRKRLKKWLKQSGERCYRLYDADMPEYALAVDVYGDRVHVQEYAPPR
SVDPRQAQRRLLDALEVIPQVLGVRPESVHYKQRTRQAGKAQYAKQDASGERFEVSEGPARLWVNLRDYLDTGLFLDHRP
VRRRLREMADGKRFLNLFCYTGTATVQAALGGASDSVSVDLSNTYLAWARDNFALNRLDPSRHRVVRDDCLRWLETAGSE
FDLIFMDPPTFSNSKKMDAVLDVQRDHARLIELAMARLAPGGTLVFSNNQRRFVLDAAVGERFAVEDISARTFDPDFSRR
PDLHHCFLIRHR

Sequences:

>Translated_732_residues
MNDVQSQPLTLYIACPRGLEGLLADELADFGAEVTGTTVAGVHAQADTAMAYRICLWSRLANRVVLCLLREQGIERPEAL
VEAARRVDWGAHLRAGSSLAVDFHGQSEHIRHTRFGAQSVKDGIVDAMRAAGRERPAVDLKTPDARIYAHLHRGRLLLGL
DLVGGSLHQRGYRRDAGHAPLKENLAAALLMRADWPARARRGEPLVDPLCGAGTLLIEAALMAADIAPQLSRDYFAFEAW
VGHDPDAWRELQREAQARANVGRRRVKSKLYGRDQSPPAIAAAKANAMRAGIPALIDLQGAEVAQLTRPEGVDGPGLVIT
NPPYGERLGELPEVVPIYTALGERLRAEFEGWQLALFTGNPDLGHRTGLRAEKQYAFKNGPLDCKLLLIPVVAQRTERET
SSEGDEPQGASGATSRPGPRNDGAQMFANRLEKNRKRLKKWLKQSGERCYRLYDADMPEYALAVDVYGDRVHVQEYAPPR
SVDPRQAQRRLLDALEVIPQVLGVRPESVHYKQRTRQAGKAQYAKQDASGERFEVSEGPARLWVNLRDYLDTGLFLDHRP
VRRRLREMADGKRFLNLFCYTGTATVQAALGGASDSVSVDLSNTYLAWARDNFALNRLDPSRHRVVRDDCLRWLETAGSE
FDLIFMDPPTFSNSKKMDAVLDVQRDHARLIELAMARLAPGGTLVFSNNQRRFVLDAAVGERFAVEDISARTFDPDFSRR
PDLHHCFLIRHR
>Mature_732_residues
MNDVQSQPLTLYIACPRGLEGLLADELADFGAEVTGTTVAGVHAQADTAMAYRICLWSRLANRVVLCLLREQGIERPEAL
VEAARRVDWGAHLRAGSSLAVDFHGQSEHIRHTRFGAQSVKDGIVDAMRAAGRERPAVDLKTPDARIYAHLHRGRLLLGL
DLVGGSLHQRGYRRDAGHAPLKENLAAALLMRADWPARARRGEPLVDPLCGAGTLLIEAALMAADIAPQLSRDYFAFEAW
VGHDPDAWRELQREAQARANVGRRRVKSKLYGRDQSPPAIAAAKANAMRAGIPALIDLQGAEVAQLTRPEGVDGPGLVIT
NPPYGERLGELPEVVPIYTALGERLRAEFEGWQLALFTGNPDLGHRTGLRAEKQYAFKNGPLDCKLLLIPVVAQRTERET
SSEGDEPQGASGATSRPGPRNDGAQMFANRLEKNRKRLKKWLKQSGERCYRLYDADMPEYALAVDVYGDRVHVQEYAPPR
SVDPRQAQRRLLDALEVIPQVLGVRPESVHYKQRTRQAGKAQYAKQDASGERFEVSEGPARLWVNLRDYLDTGLFLDHRP
VRRRLREMADGKRFLNLFCYTGTATVQAALGGASDSVSVDLSNTYLAWARDNFALNRLDPSRHRVVRDDCLRWLETAGSE
FDLIFMDPPTFSNSKKMDAVLDVQRDHARLIELAMARLAPGGTLVFSNNQRRFVLDAAVGERFAVEDISARTFDPDFSRR
PDLHHCFLIRHR

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=724, Percent_Identity=45.5801104972376, Blast_Score=600, Evalue=1e-172,
Organism=Escherichia coli, GI145693111, Length=274, Percent_Identity=31.021897810219, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_CHRSD (Q1QXV9)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573398.1
- ProteinModelPortal:   Q1QXV9
- SMR:   Q1QXV9
- STRING:   Q1QXV9
- GeneID:   4027739
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1344
- NMPDR:   fig|290398.4.peg.1334
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   CSAL290398:CSAL_1344-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 81513; Mature: 81513

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVQSQPLTLYIACPRGLEGLLADELADFGAEVTGTTVAGVHAQADTAMAYRICLWSRL
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
ANRVVLCLLREQGIERPEALVEAARRVDWGAHLRAGSSLAVDFHGQSEHIRHTRFGAQSV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHH
KDGIVDAMRAAGRERPAVDLKTPDARIYAHLHRGRLLLGLDLVGGSLHQRGYRRDAGHAP
HHHHHHHHHHCCCCCCCEECCCCCHHHHEEHHCCEEEEEEEECCCHHHHCCCCCCCCCCC
LKENLAAALLMRADWPARARRGEPLVDPLCGAGTLLIEAALMAADIAPQLSRDYFAFEAW
HHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCEEEEEE
VGHDPDAWRELQREAQARANVGRRRVKSKLYGRDQSPPAIAAAKANAMRAGIPALIDLQG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCC
AEVAQLTRPEGVDGPGLVITNPPYGERLGELPEVVPIYTALGERLRAEFEGWQLALFTGN
CHHHHCCCCCCCCCCCEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECC
PDLGHRTGLRAEKQYAFKNGPLDCKLLLIPVVAQRTERETSSEGDEPQGASGATSRPGPR
CCCCCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
NDGAQMFANRLEKNRKRLKKWLKQSGERCYRLYDADMPEYALAVDVYGDRVHVQEYAPPR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCEEEEEEECCCEEEEECCCCCC
SVDPRQAQRRLLDALEVIPQVLGVRPESVHYKQRTRQAGKAQYAKQDASGERFEVSEGPA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCE
RLWVNLRDYLDTGLFLDHRPVRRRLREMADGKRFLNLFCYTGTATVQAALGGASDSVSVD
EEEEEHHHHHCCCCEECCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHCCCCCCEEEE
LSNTYLAWARDNFALNRLDPSRHRVVRDDCLRWLETAGSEFDLIFMDPPTFSNSKKMDAV
ECCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHH
LDVQRDHARLIELAMARLAPGGTLVFSNNQRRFVLDAAVGERFAVEDISARTFDPDFSRR
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEHHCCCCCCCCCCCCCC
PDLHHCFLIRHR
CCCCEEEEEECC
>Mature Secondary Structure
MNDVQSQPLTLYIACPRGLEGLLADELADFGAEVTGTTVAGVHAQADTAMAYRICLWSRL
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
ANRVVLCLLREQGIERPEALVEAARRVDWGAHLRAGSSLAVDFHGQSEHIRHTRFGAQSV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHH
KDGIVDAMRAAGRERPAVDLKTPDARIYAHLHRGRLLLGLDLVGGSLHQRGYRRDAGHAP
HHHHHHHHHHCCCCCCCEECCCCCHHHHEEHHCCEEEEEEEECCCHHHHCCCCCCCCCCC
LKENLAAALLMRADWPARARRGEPLVDPLCGAGTLLIEAALMAADIAPQLSRDYFAFEAW
HHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCEEEEEE
VGHDPDAWRELQREAQARANVGRRRVKSKLYGRDQSPPAIAAAKANAMRAGIPALIDLQG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCC
AEVAQLTRPEGVDGPGLVITNPPYGERLGELPEVVPIYTALGERLRAEFEGWQLALFTGN
CHHHHCCCCCCCCCCCEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECC
PDLGHRTGLRAEKQYAFKNGPLDCKLLLIPVVAQRTERETSSEGDEPQGASGATSRPGPR
CCCCCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
NDGAQMFANRLEKNRKRLKKWLKQSGERCYRLYDADMPEYALAVDVYGDRVHVQEYAPPR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCEEEEEEECCCEEEEECCCCCC
SVDPRQAQRRLLDALEVIPQVLGVRPESVHYKQRTRQAGKAQYAKQDASGERFEVSEGPA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCE
RLWVNLRDYLDTGLFLDHRPVRRRLREMADGKRFLNLFCYTGTATVQAALGGASDSVSVD
EEEEEHHHHHCCCCEECCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHCCCCCCEEEE
LSNTYLAWARDNFALNRLDPSRHRVVRDDCLRWLETAGSEFDLIFMDPPTFSNSKKMDAV
ECCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHH
LDVQRDHARLIELAMARLAPGGTLVFSNNQRRFVLDAAVGERFAVEDISARTFDPDFSRR
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEHHCCCCCCCCCCCCCC
PDLHHCFLIRHR
CCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA