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The map label for this gene is purA

Identifier: 92113408

GI number: 92113408

Start: 1462803

End: 1464098

Strand: Direct

Name: purA

Synonym: Csal_1282

Alternate gene names: 92113408

Gene position: 1462803-1464098 (Clockwise)

Preceding gene: 92113407

Following gene: 92113411

Centisome position: 39.57

GC content: 64.04

Gene sequence:

>1296_bases
ATGGGCAAGAACGTAGTCGTACTGGGTACCCAATGGGGTGACGAGGGGAAAGGCAAGGTCGTTGACCTGCTGACCGAATC
CGCTGCCACGGTGGTGCGCTTTCAGGGCGGTCACAACGCCGGCCATACGCTGGTCATCGACGGCGAGAAGACCGTCCTGC
ATCTGATCCCGTCCGGCGTGCTGCGCGCCGACAAGACGTGCGTGATCGGCAATGGGGTCGTGCTCTCGCCGGAAGCGCTG
ATGGACGAGATTCGCGAGCTTGAAGCCAAGGGCGTGCCGGTGCGCGAACGCCTGCGTCTGTCGCCGGCATGTCCGCTGAT
CCTGCCGTATCACGTGCGTCTCGACCAGGCGCGCGAGAAAGCGCGCGGCATCGCCAAGATCGGCACCACTGGCCGGGGTA
TCGGGCCCGCGTACGAGGACAAGGTGGCGCGTCGGGGGCTGCGCCTGGGCGACATGCTGCATCGCGAACGCTTTGCCTCG
AAGCTCGGCGAAGTGCTCGACTATCACAATTTCGTGCTGACGCAGTACCACCACGAAGCGCCGGTGGACTTCCAGCGCGT
GCTCGACGAGGCGATGGAAATCGCCGAAGAGCTGCGGCCGATGGTCTGCGATACGGTCTCTCTGGTGCATGACACCCGCA
AGGCGGGCGAGAACATCCTCTTCGAAGGGGCCCAGGGGTCGCTGCTGGACATCGATCACGGTACTTATCCCTACGTGACC
AGCTCCAACACCACGGCGGGAGGCACGGCCACGGGGTCGGGCGTGGGTCCGCTGTACCTGGATTACGTGCTGGGCATTAC
CAAGGCGTACACCACCCGGGTGGGGTCCGGCCCGTTTCCCACCGAACTGTTCGACGAGTTCGGTCGCCATCTGGCGGAGA
AGGGGCATGAGTTCGGTGCCACCACGGGTCGCGCGCGCCGCTGCGGCTGGTTCGATGCCGTGGCACTGCGTCACGCGGTG
CAGATCAACTCGGTCTCGGGGCTGTGCCTGACCAAGCTCGACGTGCTCGACGGCCTCGAGAACATTCGTGTCTGCATCGG
CTATCGCAGCAAGGACGGCGAGACCATCGATACACCGGTCGATTCGGAAGGCTACGAAGTCATCGAGCCGCTGTATCAGG
ACCTGCCGGGCTGGAGCGAGTCGACACTGGGCGTCAAGCGGATCGAGGACCTGCCCAACAACGCACGTGCCTATATCAGC
TTCCTCGAGGAACAGACGGGCGTACCGATCGATATCATTTCGACCGGCCCCGACCGCAACGAGACGATCGTGCTGCGCAA
TCCGTTTCTGGACTGA

Upstream 100 bases:

>100_bases
CGAACAGCGCTGCGATCGGCAGCTAGTGGAATCGAACGGCGAATGGCGGGTCGAACCGCTCGCGTAAAACGGCGGATCCT
TTGATCGAGAGACGAAAGCA

Downstream 100 bases:

>100_bases
GTGCGTCACACCGAGAGAGTGAAAGACGCCCGCCCGGCACTGCCGGTCGGGCGTTCGTTCGTTGGCGACCCGTGAATGTC
GAGCGTTGCCGATGCGGTAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKNVVVLGTQWGDEGKGKVVDLLTESAATVVRFQGGHNAGHTLVIDGEKTVLHLIPSGVLRADKTCVIGNGVVLSPEAL
MDEIRELEAKGVPVRERLRLSPACPLILPYHVRLDQAREKARGIAKIGTTGRGIGPAYEDKVARRGLRLGDMLHRERFAS
KLGEVLDYHNFVLTQYHHEAPVDFQRVLDEAMEIAEELRPMVCDTVSLVHDTRKAGENILFEGAQGSLLDIDHGTYPYVT
SSNTTAGGTATGSGVGPLYLDYVLGITKAYTTRVGSGPFPTELFDEFGRHLAEKGHEFGATTGRARRCGWFDAVALRHAV
QINSVSGLCLTKLDVLDGLENIRVCIGYRSKDGETIDTPVDSEGYEVIEPLYQDLPGWSESTLGVKRIEDLPNNARAYIS
FLEEQTGVPIDIISTGPDRNETIVLRNPFLD

Sequences:

>Translated_431_residues
MGKNVVVLGTQWGDEGKGKVVDLLTESAATVVRFQGGHNAGHTLVIDGEKTVLHLIPSGVLRADKTCVIGNGVVLSPEAL
MDEIRELEAKGVPVRERLRLSPACPLILPYHVRLDQAREKARGIAKIGTTGRGIGPAYEDKVARRGLRLGDMLHRERFAS
KLGEVLDYHNFVLTQYHHEAPVDFQRVLDEAMEIAEELRPMVCDTVSLVHDTRKAGENILFEGAQGSLLDIDHGTYPYVT
SSNTTAGGTATGSGVGPLYLDYVLGITKAYTTRVGSGPFPTELFDEFGRHLAEKGHEFGATTGRARRCGWFDAVALRHAV
QINSVSGLCLTKLDVLDGLENIRVCIGYRSKDGETIDTPVDSEGYEVIEPLYQDLPGWSESTLGVKRIEDLPNNARAYIS
FLEEQTGVPIDIISTGPDRNETIVLRNPFLD
>Mature_430_residues
GKNVVVLGTQWGDEGKGKVVDLLTESAATVVRFQGGHNAGHTLVIDGEKTVLHLIPSGVLRADKTCVIGNGVVLSPEALM
DEIRELEAKGVPVRERLRLSPACPLILPYHVRLDQAREKARGIAKIGTTGRGIGPAYEDKVARRGLRLGDMLHRERFASK
LGEVLDYHNFVLTQYHHEAPVDFQRVLDEAMEIAEELRPMVCDTVSLVHDTRKAGENILFEGAQGSLLDIDHGTYPYVTS
SNTTAGGTATGSGVGPLYLDYVLGITKAYTTRVGSGPFPTELFDEFGRHLAEKGHEFGATTGRARRCGWFDAVALRHAVQ
INSVSGLCLTKLDVLDGLENIRVCIGYRSKDGETIDTPVDSEGYEVIEPLYQDLPGWSESTLGVKRIEDLPNNARAYISF
LEEQTGVPIDIISTGPDRNETIVLRNPFLD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=43.4272300469484, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=42.2273781902552, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=41.3098236775819, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=68.1395348837209, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=42.4882629107981, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=42.4882629107981, Blast_Score=336, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=43.2558139534884, Blast_Score=343, Evalue=2e-95,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=43.2304038004751, Blast_Score=341, Evalue=7e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_CHRSD (Q1QY21)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573336.1
- ProteinModelPortal:   Q1QY21
- SMR:   Q1QY21
- STRING:   Q1QY21
- GeneID:   4027755
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1282
- NMPDR:   fig|290398.4.peg.1272
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- BioCyc:   CSAL290398:CSAL_1282-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47099; Mature: 46968

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKVVDLLTESAATVVRFQGGHNAGHTLVIDGEKTVLHLIPSGV
CCCCEEEEEECCCCCCCCCEEHHHHHCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCH
LRADKTCVIGNGVVLSPEALMDEIRELEAKGVPVRERLRLSPACPLILPYHVRLDQAREK
HCCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHH
ARGIAKIGTTGRGIGPAYEDKVARRGLRLGDMLHRERFASKLGEVLDYHNFVLTQYHHEA
HCCHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
PVDFQRVLDEAMEIAEELRPMVCDTVSLVHDTRKAGENILFEGAQGSLLDIDHGTYPYVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGTATGSGVGPLYLDYVLGITKAYTTRVGSGPFPTELFDEFGRHLAEKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
TTGRARRCGWFDAVALRHAVQINSVSGLCLTKLDVLDGLENIRVCIGYRSKDGETIDTPV
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
DSEGYEVIEPLYQDLPGWSESTLGVKRIEDLPNNARAYISFLEEQTGVPIDIISTGPDRN
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
ETIVLRNPFLD
CEEEEECCCCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKVVDLLTESAATVVRFQGGHNAGHTLVIDGEKTVLHLIPSGV
CCCEEEEEECCCCCCCCCEEHHHHHCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCH
LRADKTCVIGNGVVLSPEALMDEIRELEAKGVPVRERLRLSPACPLILPYHVRLDQAREK
HCCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHH
ARGIAKIGTTGRGIGPAYEDKVARRGLRLGDMLHRERFASKLGEVLDYHNFVLTQYHHEA
HCCHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
PVDFQRVLDEAMEIAEELRPMVCDTVSLVHDTRKAGENILFEGAQGSLLDIDHGTYPYVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGTATGSGVGPLYLDYVLGITKAYTTRVGSGPFPTELFDEFGRHLAEKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
TTGRARRCGWFDAVALRHAVQINSVSGLCLTKLDVLDGLENIRVCIGYRSKDGETIDTPV
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
DSEGYEVIEPLYQDLPGWSESTLGVKRIEDLPNNARAYISFLEEQTGVPIDIISTGPDRN
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
ETIVLRNPFLD
CEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA