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The map label for this gene is folC [H]

Identifier: 92113390

GI number: 92113390

Start: 1442519

End: 1443832

Strand: Direct

Name: folC [H]

Synonym: Csal_1264

Alternate gene names: 92113390

Gene position: 1442519-1443832 (Clockwise)

Preceding gene: 92113389

Following gene: 92113391

Centisome position: 39.02

GC content: 69.33

Gene sequence:

>1314_bases
ATGACTGCCGCGACCTCCCTGCAGGCCTGGCTGGCGCGCCTCGAACGCCAGCATCCCGTGGCGATCGACCTGGGCTTGGA
ACGCGTGGCCGAGGTCGCGCGGCGGCTGGCGCTGCTCGATACGCCCTTGGCCACGCGCGTGATCACCGTGGCCGGGACCA
ACGGCAAGGGGTCGACCGTGGCCATGCTCGAGGCAGTCGCTCGCGAGCATGGCCTGCGGACCGCCGCCTATACCTCCCCC
CATCTACTGCGTTACAACGAGCGCCTGCGTCTTGCAGGGTGCGAAGCCGACGATGCCGCCTTGGTGGAGGGCTTCGAAGC
CGTCGAGGCAGCGCGCCTCGACGGCGATCCGGTCACGCTCACGTATTTCGAGGCGGGCACGCTGGCGGCGCTCTGGTTGA
TCGCGCGCTGGCAGCCGGATGTCGCGCTTCTCGAAGTCGGTCTGGGCGGGCGCCTGGATGCGGTCAATGTCATCGACCCG
GACGTGGCCGTGGTCACGACGGTGGCTCAGGACCATGCGGCGTTTCTGGGGACCCATCTCGACACGATAGGCCGGGAAAA
GGCCGGTATCCTGCGTGCGGGACGGCCCGCGGTGCTGGGCAGCACGACGTTGCCCGCCAGTGTGCGCGAACGTGCCGACG
CACTGTCAGCGCCGGTTTTCGCGCTCCAGGAGGCCTTTGCCTGGCGCATGGGTGACCGCGGCCAGGAGGGGAGCGAGTGG
CAATGGCAAGGCACCGCGCCCGGTGGCGAGAAGGTGGCGCTGCACGGCCTGCCGGATCCCGGCCTGCCGCTGGACAATGC
CGCGGTCGCCCTGCAGACCCTGGCGCTGGCCGACGTGAAGCTCGACGAAACCGCTACCCGACGCGCCCTGTCGCGGGTGG
TGCTGGCCGGTCGTCTGCAATGGATGGGTCCCTGGTGTCTGGACGTCGGGCACAACCCGCATGCCGCCGCCTATGTGGCC
GGGCAGCTCGCGCAGCGCCCACGTCGCGGGCGGCGCTTGTGTCTGCTGGGCATGCTGGCCGACAAGGATGCCGATGGGGT
CATCGCGGCGCTGGCGCCGCAGATCGATGCCTGGGTCACCGTCTCTCTGAGCGGGGATCGGGCGCGTGATGCGGCGCAGC
TGGCGACGTTTGTGGAAGGGCGGGGCGCCAAGGTCCTGCATCAGGGAGCCTCGCCCGAGGACGGCGTGCGGTGGTTGCAT
GCGAACATGGACAATGGCGACGACATTCTGGTCTGTGGATCGTTCTGGACCGTTGCCGAGGCGCTTGCCGCACTGGAAAC
GCTGGGTGCGGCGAGTTCAAGGAGTGCGTCATGA

Upstream 100 bases:

>100_bases
GCGCAAGTTGACACACCAGCCGCATCAGGATCGTGATGCCGAGCCCGACGACACGGCATCGCAATCGACACTGGACGAGT
TCTCCCAGGCCGATCACTGA

Downstream 100 bases:

>100_bases
AGTACGGAATGCGTGAGCGCATCAGCGGCGCCATTATCCTGGTGGCGCTGGCGGTGATCTTCTTGCCGATGCTGTTCGAT
GAGCCGACGCCGCGTAGCGA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTAATSLQAWLARLERQHPVAIDLGLERVAEVARRLALLDTPLATRVITVAGTNGKGSTVAMLEAVAREHGLRTAAYTSP
HLLRYNERLRLAGCEADDAALVEGFEAVEAARLDGDPVTLTYFEAGTLAALWLIARWQPDVALLEVGLGGRLDAVNVIDP
DVAVVTTVAQDHAAFLGTHLDTIGREKAGILRAGRPAVLGSTTLPASVRERADALSAPVFALQEAFAWRMGDRGQEGSEW
QWQGTAPGGEKVALHGLPDPGLPLDNAAVALQTLALADVKLDETATRRALSRVVLAGRLQWMGPWCLDVGHNPHAAAYVA
GQLAQRPRRGRRLCLLGMLADKDADGVIAALAPQIDAWVTVSLSGDRARDAAQLATFVEGRGAKVLHQGASPEDGVRWLH
ANMDNGDDILVCGSFWTVAEALAALETLGAASSRSAS

Sequences:

>Translated_437_residues
MTAATSLQAWLARLERQHPVAIDLGLERVAEVARRLALLDTPLATRVITVAGTNGKGSTVAMLEAVAREHGLRTAAYTSP
HLLRYNERLRLAGCEADDAALVEGFEAVEAARLDGDPVTLTYFEAGTLAALWLIARWQPDVALLEVGLGGRLDAVNVIDP
DVAVVTTVAQDHAAFLGTHLDTIGREKAGILRAGRPAVLGSTTLPASVRERADALSAPVFALQEAFAWRMGDRGQEGSEW
QWQGTAPGGEKVALHGLPDPGLPLDNAAVALQTLALADVKLDETATRRALSRVVLAGRLQWMGPWCLDVGHNPHAAAYVA
GQLAQRPRRGRRLCLLGMLADKDADGVIAALAPQIDAWVTVSLSGDRARDAAQLATFVEGRGAKVLHQGASPEDGVRWLH
ANMDNGDDILVCGSFWTVAEALAALETLGAASSRSAS
>Mature_436_residues
TAATSLQAWLARLERQHPVAIDLGLERVAEVARRLALLDTPLATRVITVAGTNGKGSTVAMLEAVAREHGLRTAAYTSPH
LLRYNERLRLAGCEADDAALVEGFEAVEAARLDGDPVTLTYFEAGTLAALWLIARWQPDVALLEVGLGGRLDAVNVIDPD
VAVVTTVAQDHAAFLGTHLDTIGREKAGILRAGRPAVLGSTTLPASVRERADALSAPVFALQEAFAWRMGDRGQEGSEWQ
WQGTAPGGEKVALHGLPDPGLPLDNAAVALQTLALADVKLDETATRRALSRVVLAGRLQWMGPWCLDVGHNPHAAAYVAG
QLAQRPRRGRRLCLLGMLADKDADGVIAALAPQIDAWVTVSLSGDRARDAAQLATFVEGRGAKVLHQGASPEDGVRWLHA
NMDNGDDILVCGSFWTVAEALAALETLGAASSRSAS

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=182, Percent_Identity=35.7142857142857, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI66932990, Length=183, Percent_Identity=35.5191256830601, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1788654, Length=431, Percent_Identity=41.7633410672854, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71984923, Length=389, Percent_Identity=29.5629820051414, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=389, Percent_Identity=29.5629820051414, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=389, Percent_Identity=29.5629820051414, Blast_Score=118, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=432, Percent_Identity=25.9259259259259, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324815, Length=202, Percent_Identity=30.6930693069307, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641571, Length=209, Percent_Identity=32.0574162679426, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24581568, Length=172, Percent_Identity=32.5581395348837, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46260; Mature: 46129

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATSLQAWLARLERQHPVAIDLGLERVAEVARRLALLDTPLATRVITVAGTNGKGSTV
CCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHH
AMLEAVAREHGLRTAAYTSPHLLRYNERLRLAGCEADDAALVEGFEAVEAARLDGDPVTL
HHHHHHHHHHCCEEEECCCCHHEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
TYFEAGTLAALWLIARWQPDVALLEVGLGGRLDAVNVIDPDVAVVTTVAQDHAAFLGTHL
EEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHH
DTIGREKAGILRAGRPAVLGSTTLPASVRERADALSAPVFALQEAFAWRMGDRGQEGSEW
HHHCCHHHCCEECCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCE
QWQGTAPGGEKVALHGLPDPGLPLDNAAVALQTLALADVKLDETATRRALSRVVLAGRLQ
EEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
WMGPWCLDVGHNPHAAAYVAGQLAQRPRRGRRLCLLGMLADKDADGVIAALAPQIDAWVT
HCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEECCCCCCEEEE
VSLSGDRARDAAQLATFVEGRGAKVLHQGASPEDGVRWLHANMDNGDDILVCGSFWTVAE
EEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCCEEEECCHHHHHH
ALAALETLGAASSRSAS
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TAATSLQAWLARLERQHPVAIDLGLERVAEVARRLALLDTPLATRVITVAGTNGKGSTV
CCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHH
AMLEAVAREHGLRTAAYTSPHLLRYNERLRLAGCEADDAALVEGFEAVEAARLDGDPVTL
HHHHHHHHHHCCEEEECCCCHHEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
TYFEAGTLAALWLIARWQPDVALLEVGLGGRLDAVNVIDPDVAVVTTVAQDHAAFLGTHL
EEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHH
DTIGREKAGILRAGRPAVLGSTTLPASVRERADALSAPVFALQEAFAWRMGDRGQEGSEW
HHHCCHHHCCEECCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCE
QWQGTAPGGEKVALHGLPDPGLPLDNAAVALQTLALADVKLDETATRRALSRVVLAGRLQ
EEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
WMGPWCLDVGHNPHAAAYVAGQLAQRPRRGRRLCLLGMLADKDADGVIAALAPQIDAWVT
HCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEECCCCCCEEEE
VSLSGDRARDAAQLATFVEGRGAKVLHQGASPEDGVRWLHANMDNGDDILVCGSFWTVAE
EEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCCEEEECCHHHHHH
ALAALETLGAASSRSAS
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]