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The map label for this gene is puuA [H]

Identifier: 92113308

GI number: 92113308

Start: 1353619

End: 1354974

Strand: Direct

Name: puuA [H]

Synonym: Csal_1181

Alternate gene names: 92113308

Gene position: 1353619-1354974 (Clockwise)

Preceding gene: 92113306

Following gene: 92113309

Centisome position: 36.62

GC content: 61.06

Gene sequence:

>1356_bases
ATGCGTATGACGACGGATAAAAAACAGGGCAAGTCCCGCGAGCTGGATCTCTTTATCACGGATTTGAACGGCAACTTGCG
CGGCAAGCGTCTGCCCGCCAGCGGCTTGAAGAAACTCCGCAAGGAAGGGCTCAAACTACCGCTCTCGGTAGTGGGCTTCG
ATTTCTGGGGGGCGGATGTTCTGGACAATGGCCTGGTATTCGAGACCGGTGACAGTGACGGCGTCTGCATGCCTGTCAGC
CAAGAGCCGATTCCGGTGCCCTGGTCGGAAGCGCCGCGTGACCAGATGCTGGCGATGATGTTCAATCCCGACGGTACGCC
CTTCGGGGCCGATCCTCGTCAGGTGCTCAACCGCATGGTCGATCGCTTCTCCGCAAAGGGGCTGACGCCGGTACTGGCGA
CCGAACTCGAGTTTTACCTGATGGATGCCGAATCGGAAAGCGTGCAGCGTCCGATACCGCCGGCACTGGCCGATGGCAAA
GGGCGTCGTCTCGCCAATGCGGAAGGTTATTCCGTGGAGGAAATGGACGGCTTCGAGGCCTTCTTCGCGGATATCCGTGA
GGCCTGCCAGGGACAGGGGATCGGCGCCGACACCATCATTGCCGAGATGGGCCCGGGGCAGTTCGAGGTGAACCTCAACC
ACGTGGCCGATCCGCTGAATGCGGGCGATCAGGCGATTCTCTTCAAGCGCCTCGTGCGTGGTGTTTCCCGCCGCCACGGC
TATGCGGCGACCTTCATGGCCAAGCCTTACGTCGAGGAAAGCGGCAACGGCTTTCACACGCATTTCAGCCTGCTCGACCG
CGACGGCAACAATGTTTTCGACGACGGTACCGAGCAGGGCAGTGACCTGTTGCGCCACGCCATCGCCGGGATGATGCAGT
TGATGCCGGAGAGCATGCTGGTGTTCGCGCCGCATCTGAATTCCTATCGTCGCTTCATGCCGGGCGCTCATGCACCGACG
CATGCCAGCTGGGGGTACGAGAACCGAACGGTGGCCCTGCGTGTGCCGGAAAGCCCCAACCTTGCTCGCCGTATCGAACA
CCGAGTGGCAGGGGCCGATGCCAATCCGTATCTGGTATTGGCATCCATTCTCGCCGGTGCGCTGCATGGCATGGAAAACG
CACTCGAGCCGGATGCCCCGGTGGAAGGTGATGCCTACGCGGAAGAAAGTCCCCGCTATCCACTGCCGTGCGAGTGGCAG
GATGCGATCGCTCGCTTCGAGCAGAGCGAGATTTTGAGGGAATACCTGGGCGAAGAGTTCGTGCGTGTCTACGCGCAAGC
CAAGCGCCAGGAGCGCCGCCGGCTGAACGAGCGCATCTCCGATGTTGAATATGAGGCCTATCTGGGATTGCTCTGA

Upstream 100 bases:

>100_bases
GTCGTCTTCCGGCGGCCGAATACCCCCAGGGTGATAGATACAGCATTATCTGGATGGGATACCTTAAGGCTCTGGTTTTC
GAAAAAAGCTCTCAAGAGTC

Downstream 100 bases:

>100_bases
TCCCGGCCAGGCCTGAAAAATTCACGGCGCGGCCGTGAGCGAGGAATCCGTCATGACACTGAAGATCGGCATACTGGCCA
CCGGCACGACGCCGGACAAT

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRMTTDKKQGKSRELDLFITDLNGNLRGKRLPASGLKKLRKEGLKLPLSVVGFDFWGADVLDNGLVFETGDSDGVCMPVS
QEPIPVPWSEAPRDQMLAMMFNPDGTPFGADPRQVLNRMVDRFSAKGLTPVLATELEFYLMDAESESVQRPIPPALADGK
GRRLANAEGYSVEEMDGFEAFFADIREACQGQGIGADTIIAEMGPGQFEVNLNHVADPLNAGDQAILFKRLVRGVSRRHG
YAATFMAKPYVEESGNGFHTHFSLLDRDGNNVFDDGTEQGSDLLRHAIAGMMQLMPESMLVFAPHLNSYRRFMPGAHAPT
HASWGYENRTVALRVPESPNLARRIEHRVAGADANPYLVLASILAGALHGMENALEPDAPVEGDAYAEESPRYPLPCEWQ
DAIARFEQSEILREYLGEEFVRVYAQAKRQERRRLNERISDVEYEAYLGLL

Sequences:

>Translated_451_residues
MRMTTDKKQGKSRELDLFITDLNGNLRGKRLPASGLKKLRKEGLKLPLSVVGFDFWGADVLDNGLVFETGDSDGVCMPVS
QEPIPVPWSEAPRDQMLAMMFNPDGTPFGADPRQVLNRMVDRFSAKGLTPVLATELEFYLMDAESESVQRPIPPALADGK
GRRLANAEGYSVEEMDGFEAFFADIREACQGQGIGADTIIAEMGPGQFEVNLNHVADPLNAGDQAILFKRLVRGVSRRHG
YAATFMAKPYVEESGNGFHTHFSLLDRDGNNVFDDGTEQGSDLLRHAIAGMMQLMPESMLVFAPHLNSYRRFMPGAHAPT
HASWGYENRTVALRVPESPNLARRIEHRVAGADANPYLVLASILAGALHGMENALEPDAPVEGDAYAEESPRYPLPCEWQ
DAIARFEQSEILREYLGEEFVRVYAQAKRQERRRLNERISDVEYEAYLGLL
>Mature_451_residues
MRMTTDKKQGKSRELDLFITDLNGNLRGKRLPASGLKKLRKEGLKLPLSVVGFDFWGADVLDNGLVFETGDSDGVCMPVS
QEPIPVPWSEAPRDQMLAMMFNPDGTPFGADPRQVLNRMVDRFSAKGLTPVLATELEFYLMDAESESVQRPIPPALADGK
GRRLANAEGYSVEEMDGFEAFFADIREACQGQGIGADTIIAEMGPGQFEVNLNHVADPLNAGDQAILFKRLVRGVSRRHG
YAATFMAKPYVEESGNGFHTHFSLLDRDGNNVFDDGTEQGSDLLRHAIAGMMQLMPESMLVFAPHLNSYRRFMPGAHAPT
HASWGYENRTVALRVPESPNLARRIEHRVAGADANPYLVLASILAGALHGMENALEPDAPVEGDAYAEESPRYPLPCEWQ
DAIARFEQSEILREYLGEEFVRVYAQAKRQERRRLNERISDVEYEAYLGLL

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=425, Percent_Identity=24.9411764705882, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI87081870, Length=438, Percent_Identity=44.7488584474886, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1790301, Length=473, Percent_Identity=25.5813953488372, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17554028, Length=334, Percent_Identity=23.3532934131737, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540008, Length=341, Percent_Identity=24.0469208211144, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50133; Mature: 50133

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMTTDKKQGKSRELDLFITDLNGNLRGKRLPASGLKKLRKEGLKLPLSVVGFDFWGADV
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHEEECCCCHHH
LDNGLVFETGDSDGVCMPVSQEPIPVPWSEAPRDQMLAMMFNPDGTPFGADPRQVLNRMV
HCCCCEEECCCCCCEEEECCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHH
DRFSAKGLTPVLATELEFYLMDAESESVQRPIPPALADGKGRRLANAEGYSVEEMDGFEA
HHHHCCCCCHHHHHCEEEEEECCCCHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHH
FFADIREACQGQGIGADTIIAEMGPGQFEVNLNHVADPLNAGDQAILFKRLVRGVSRRHG
HHHHHHHHHCCCCCCHHHHHEECCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
YAATFMAKPYVEESGNGFHTHFSLLDRDGNNVFDDGTEQGSDLLRHAIAGMMQLMPESML
CEEEEEECCCHHCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHE
VFAPHLNSYRRFMPGAHAPTHASWGYENRTVALRVPESPNLARRIEHRVAGADANPYLVL
EECCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHH
ASILAGALHGMENALEPDAPVEGDAYAEESPRYPLPCEWQDAIARFEQSEILREYLGEEF
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VRVYAQAKRQERRRLNERISDVEYEAYLGLL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MRMTTDKKQGKSRELDLFITDLNGNLRGKRLPASGLKKLRKEGLKLPLSVVGFDFWGADV
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHEEECCCCHHH
LDNGLVFETGDSDGVCMPVSQEPIPVPWSEAPRDQMLAMMFNPDGTPFGADPRQVLNRMV
HCCCCEEECCCCCCEEEECCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHH
DRFSAKGLTPVLATELEFYLMDAESESVQRPIPPALADGKGRRLANAEGYSVEEMDGFEA
HHHHCCCCCHHHHHCEEEEEECCCCHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHH
FFADIREACQGQGIGADTIIAEMGPGQFEVNLNHVADPLNAGDQAILFKRLVRGVSRRHG
HHHHHHHHHCCCCCCHHHHHEECCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
YAATFMAKPYVEESGNGFHTHFSLLDRDGNNVFDDGTEQGSDLLRHAIAGMMQLMPESML
CEEEEEECCCHHCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHE
VFAPHLNSYRRFMPGAHAPTHASWGYENRTVALRVPESPNLARRIEHRVAGADANPYLVL
EECCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHH
ASILAGALHGMENALEPDAPVEGDAYAEESPRYPLPCEWQDAIARFEQSEILREYLGEEF
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VRVYAQAKRQERRRLNERISDVEYEAYLGLL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]