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The map label for this gene is pleD [H]

Identifier: 92113187

GI number: 92113187

Start: 1196871

End: 1198583

Strand: Direct

Name: pleD [H]

Synonym: Csal_1059

Alternate gene names: 92113187

Gene position: 1196871-1198583 (Clockwise)

Preceding gene: 92113176

Following gene: 92113189

Centisome position: 32.38

GC content: 51.61

Gene sequence:

>1713_bases
ATGCCCGAGAAAGCGTGGTTCAAGCAGGTCATGCAGGATTCGGAAGACGCATACCTTTTCATCTCGAAAGACAGCATGAT
CGAAGATGTGGCAGGGGCTATCCAGAATGTCTTGAAGGTGCCAAGAAAAGAGCTTGTTGGTGCCGCGTTATCCGATCGGG
ATATTTTGCGAGCGGATTTCCTGAAAAGTTTCATCGCAGAGTCCGTCAAAGCGCCGTGCTCCAGCGACAAGAAAAACGAT
GCCTATCTGGATCGGCGGAACCAGTTGCGCTGGAAATACTATGAAGCCAGAGATGGCAATGTCGTAGCCAAAGTCTCTCG
CATGAATACCCACTTGGAGGCTGAAGAAGCGATCGTCGATCAGCTCGCGGATGCGCTGCCGATGATGATTACTTATGTCG
ACAGGAATTGGTGCTTTCTTTTCAACAATAAAGCCTATGAAGAATTCATCGGGCTGACCCGTGAGGAGTTGTACCGAAAG
CCTGTCTCCTCGGTCATGACCGAGGAGTCATTCAAGAAGATCGTGCCGCGTTTTGAGAGAGCTCTGAGTGGAGAGCGAGT
CAACTATGAAGATAGGTTGACTCTTGCCGATGGCCGGTCTCTTCACTTCAACGTCAGCTATATTCCTTATTTTATTGGTG
ATGAAGTCGGTGGTTTCTATGCCGTCATCAATGATGTCTCGGAATACAGCGCGATGATCAATCTTCTAAAGGGCATTCAT
AACGCTGTCAACCGAACGGATATTGATACACAAGAGATTATCAGCAATCTGCTGCGTGATGCACTGGATTATCTGGGGCA
GGATATCGCGATCGTCAGCCAGGTGGTGGGGGATCAGTACACCATCATGTGGAGCGAATCCAAGGAAGCAGCGATTGAGC
CGGGTACGGTATTTTCCCTTGGAGATACATACTGCCGTCTGACGCTGAACCATGATGATGTGTTTCACACCATCGATGCA
GGCAATGATATACGCTTTTCAGGCCATCCTTGCTATGAAACGTTTGGCTTGGAAACCTACATCGGAGCCCCCGTGCGCAT
AAATGGCATTGTCTGGGGGACCGTCAACTTCTCCAGCGCTTCGCATCGCGTGAAACCCTTCAGTGATATCGAAGTGGAGC
TCGTGCGCTTGATCGTGGGGGCAGTCGAGAAGGTAGTAACGCAATTCGAGTATTTATCGCAGGTCAAAAAAGAGCGCGAT
GAAATGGCGGCGCTGGCAAGAAAGGACCAGTTGACGGGGTTACCTAATCGAGCATACCTCAACGAGTATGTCGACATGCT
CGTCAAGAGTCCAGACGTTCAACACGCCGGTATTTGTCTTGCCGTCATCGACATCGATCATTTCAAAACCATCAACGACA
CCTATGGCCATGATATTGGCGACCAAGTGATCATCTGGCTCTCGGAGCGCGTCACGGAAGTTCTTCGCGCTCGAGCGGGT
GACTTCGCGGCACGCGTGGGCGGTGAGGAATTCGTCGTCATGATCAATAACTCGTCACTGGAGGTGGCTCGCAGCGTGAT
GGAACGCGTGAGATCGAAGATCGAGTCGACGCCGATTCCCATCGAGGAAGCGTCCCCGCTTCACATCACGATCAGCAGTG
GCGTCACTCAGTGGCAGCCCACCGACACATATCCACATGTGTTCAAACGTGCCGACGAAGCGCTGTACGAGGCGAAGCAC
GGTGGGCGCAATCAGGTTTGTGTGAGCCAGTAG

Upstream 100 bases:

>100_bases
TTCTTTTACTCATGAACCCACTAAGGTCAGGGTATGACAGATACCCATGCACTAGATATGCTGCATGTATCAGGATGATG
ACAAGGCCAGGGAGCAGCCC

Downstream 100 bases:

>100_bases
AGCTGTCAATAAAGTGCGGCTCTCCTTTCGCACTGAGCAAGATTTCCCATCGTGCGAGTATCTGGCACCACCCCCAGTGA
GGTAGGGCGTCGATGTCATA

Product: PAS/PAC and GAF sensor-containing diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MPEKAWFKQVMQDSEDAYLFISKDSMIEDVAGAIQNVLKVPRKELVGAALSDRDILRADFLKSFIAESVKAPCSSDKKND
AYLDRRNQLRWKYYEARDGNVVAKVSRMNTHLEAEEAIVDQLADALPMMITYVDRNWCFLFNNKAYEEFIGLTREELYRK
PVSSVMTEESFKKIVPRFERALSGERVNYEDRLTLADGRSLHFNVSYIPYFIGDEVGGFYAVINDVSEYSAMINLLKGIH
NAVNRTDIDTQEIISNLLRDALDYLGQDIAIVSQVVGDQYTIMWSESKEAAIEPGTVFSLGDTYCRLTLNHDDVFHTIDA
GNDIRFSGHPCYETFGLETYIGAPVRINGIVWGTVNFSSASHRVKPFSDIEVELVRLIVGAVEKVVTQFEYLSQVKKERD
EMAALARKDQLTGLPNRAYLNEYVDMLVKSPDVQHAGICLAVIDIDHFKTINDTYGHDIGDQVIIWLSERVTEVLRARAG
DFAARVGGEEFVVMINNSSLEVARSVMERVRSKIESTPIPIEEASPLHITISSGVTQWQPTDTYPHVFKRADEALYEAKH
GGRNQVCVSQ

Sequences:

>Translated_570_residues
MPEKAWFKQVMQDSEDAYLFISKDSMIEDVAGAIQNVLKVPRKELVGAALSDRDILRADFLKSFIAESVKAPCSSDKKND
AYLDRRNQLRWKYYEARDGNVVAKVSRMNTHLEAEEAIVDQLADALPMMITYVDRNWCFLFNNKAYEEFIGLTREELYRK
PVSSVMTEESFKKIVPRFERALSGERVNYEDRLTLADGRSLHFNVSYIPYFIGDEVGGFYAVINDVSEYSAMINLLKGIH
NAVNRTDIDTQEIISNLLRDALDYLGQDIAIVSQVVGDQYTIMWSESKEAAIEPGTVFSLGDTYCRLTLNHDDVFHTIDA
GNDIRFSGHPCYETFGLETYIGAPVRINGIVWGTVNFSSASHRVKPFSDIEVELVRLIVGAVEKVVTQFEYLSQVKKERD
EMAALARKDQLTGLPNRAYLNEYVDMLVKSPDVQHAGICLAVIDIDHFKTINDTYGHDIGDQVIIWLSERVTEVLRARAG
DFAARVGGEEFVVMINNSSLEVARSVMERVRSKIESTPIPIEEASPLHITISSGVTQWQPTDTYPHVFKRADEALYEAKH
GGRNQVCVSQ
>Mature_569_residues
PEKAWFKQVMQDSEDAYLFISKDSMIEDVAGAIQNVLKVPRKELVGAALSDRDILRADFLKSFIAESVKAPCSSDKKNDA
YLDRRNQLRWKYYEARDGNVVAKVSRMNTHLEAEEAIVDQLADALPMMITYVDRNWCFLFNNKAYEEFIGLTREELYRKP
VSSVMTEESFKKIVPRFERALSGERVNYEDRLTLADGRSLHFNVSYIPYFIGDEVGGFYAVINDVSEYSAMINLLKGIHN
AVNRTDIDTQEIISNLLRDALDYLGQDIAIVSQVVGDQYTIMWSESKEAAIEPGTVFSLGDTYCRLTLNHDDVFHTIDAG
NDIRFSGHPCYETFGLETYIGAPVRINGIVWGTVNFSSASHRVKPFSDIEVELVRLIVGAVEKVVTQFEYLSQVKKERDE
MAALARKDQLTGLPNRAYLNEYVDMLVKSPDVQHAGICLAVIDIDHFKTINDTYGHDIGDQVIIWLSERVTEVLRARAGD
FAARVGGEEFVVMINNSSLEVARSVMERVRSKIESTPIPIEEASPLHITISSGVTQWQPTDTYPHVFKRADEALYEAKHG
GRNQVCVSQ

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=194, Percent_Identity=36.0824742268041, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=37.9518072289157, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87081881, Length=185, Percent_Identity=33.5135135135135, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786584, Length=182, Percent_Identity=31.3186813186813, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI145693134, Length=220, Percent_Identity=28.6363636363636, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081977, Length=228, Percent_Identity=27.6315789473684, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787541, Length=181, Percent_Identity=30.3867403314917, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=34.8101265822785, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=30.939226519337, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1787802, Length=239, Percent_Identity=28.4518828451883, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081974, Length=153, Percent_Identity=30.718954248366, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=32.2033898305085, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=223, Percent_Identity=25.1121076233184, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=167, Percent_Identity=28.7425149700599, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 64454; Mature: 64322

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKAWFKQVMQDSEDAYLFISKDSMIEDVAGAIQNVLKVPRKELVGAALSDRDILRADF
CCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHH
LKSFIAESVKAPCSSDKKNDAYLDRRNQLRWKYYEARDGNVVAKVSRMNTHLEAEEAIVD
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCEEEEEHHHHCCHHHHHHHHH
QLADALPMMITYVDRNWCFLFNNKAYEEFIGLTREELYRKPVSSVMTEESFKKIVPRFER
HHHHHHHHHHEEECCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSGERVNYEDRLTLADGRSLHFNVSYIPYFIGDEVGGFYAVINDVSEYSAMINLLKGIH
HHCCCCCCCCCCEEEECCCEEEEEEEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
NAVNRTDIDTQEIISNLLRDALDYLGQDIAIVSQVVGDQYTIMWSESKEAAIEPGTVFSL
HHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEC
GDTYCRLTLNHDDVFHTIDAGNDIRFSGHPCYETFGLETYIGAPVRINGIVWGTVNFSSA
CCCEEEEEECCCCEEEEECCCCEEEECCCCCHHHCCCHHHCCCCEEEEEEEEEEEEECCC
SHRVKPFSDIEVELVRLIVGAVEKVVTQFEYLSQVKKERDEMAALARKDQLTGLPNRAYL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
NEYVDMLVKSPDVQHAGICLAVIDIDHFKTINDTYGHDIGDQVIIWLSERVTEVLRARAG
HHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCC
DFAARVGGEEFVVMINNSSLEVARSVMERVRSKIESTPIPIEEASPLHITISSGVTQWQP
CHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCC
TDTYPHVFKRADEALYEAKHGGRNQVCVSQ
CCCHHHHHHHHHHHHHHHHCCCCCCEECCC
>Mature Secondary Structure 
PEKAWFKQVMQDSEDAYLFISKDSMIEDVAGAIQNVLKVPRKELVGAALSDRDILRADF
CCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHH
LKSFIAESVKAPCSSDKKNDAYLDRRNQLRWKYYEARDGNVVAKVSRMNTHLEAEEAIVD
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCEEEEEHHHHCCHHHHHHHHH
QLADALPMMITYVDRNWCFLFNNKAYEEFIGLTREELYRKPVSSVMTEESFKKIVPRFER
HHHHHHHHHHEEECCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSGERVNYEDRLTLADGRSLHFNVSYIPYFIGDEVGGFYAVINDVSEYSAMINLLKGIH
HHCCCCCCCCCCEEEECCCEEEEEEEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
NAVNRTDIDTQEIISNLLRDALDYLGQDIAIVSQVVGDQYTIMWSESKEAAIEPGTVFSL
HHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEC
GDTYCRLTLNHDDVFHTIDAGNDIRFSGHPCYETFGLETYIGAPVRINGIVWGTVNFSSA
CCCEEEEEECCCCEEEEECCCCEEEECCCCCHHHCCCHHHCCCCEEEEEEEEEEEEECCC
SHRVKPFSDIEVELVRLIVGAVEKVVTQFEYLSQVKKERDEMAALARKDQLTGLPNRAYL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
NEYVDMLVKSPDVQHAGICLAVIDIDHFKTINDTYGHDIGDQVIIWLSERVTEVLRARAG
HHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCC
DFAARVGGEEFVVMINNSSLEVARSVMERVRSKIESTPIPIEEASPLHITISSGVTQWQP
CHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCC
TDTYPHVFKRADEALYEAKHGGRNQVCVSQ
CCCHHHHHHHHHHHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA