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The map label for this gene is dnaB [H]

Identifier: 92113020

GI number: 92113020

Start: 1003709

End: 1005097

Strand: Direct

Name: dnaB [H]

Synonym: Csal_0892

Alternate gene names: 92113020

Gene position: 1003709-1005097 (Clockwise)

Preceding gene: 92113019

Following gene: 92113021

Centisome position: 27.15

GC content: 62.99

Gene sequence:

>1389_bases
ATGAGCGAATACGTCGAGCACGACCAGGAAACCGCTGCCCTCAAGGTACCGCCGCACTCCCTGGAGGCCGAGCAGTCGGT
ACTGGGGGGGCTGATGCTGGAGAACAGCGCCTGGGATACCGTCTCGGAGCGTCTCTCGGCCGACGATTTCTATCGCTACG
AGCATCGACTGATCTTCAACGCCATGGCCCAGCTCGCCGATGCGGGCCATCCGCTCGACGTGGTCACGCTGTCCGAAGCG
CTGGAAAATCGCGACCAGCTGGAAAGCGTCGGCGGTCTGGCGTATCTGGCCGAACTGGCACGCAACACGCCTTCGGCGAG
CAACATCCGCGCCTATACCGATATCGTTCGCGAGCGGGCGACCCTGCGCAAGCTGATACAGGCCGCTCATCAGATCGCCG
ACGGGGCGTTCGCGCCGCAGGGGCGTCCCGCCGATGAACTGGTCAACGAAGCCGAGCGTCTGGTCTTCCAGATCAGCGAG
GATCGTCCCAAGACCGGCGGTGCGATCGGCATGAGCGATCTGCTCAGCAAGGCCGTCGATCGCATCGACGAACTGTTCAA
CATGCAGGGTGAGATGACCGGCCTGTCGACCGGCTTCCGGGATCTCGACGAGATGACCTCGGGGCTGCAGCCGTCTGACC
TGGTGATCATCGCCGGCCGGCCGTCGATGGGCAAGACGACGTTCGCCATGAACGTGGTCGAGCATGCGGTCATTTCCAGC
GACAAGCCGGTGGTGGTGTTTTCCATGGAGATGCCCGCCGATGCCCTGATGCTGCGCATGCTCTCGTCGCTGGGACGCAT
CGACCAGACCCGAGTGCGTACCGGCCAGCTCGAGGACGAGGATTGGCCACGTCTGACCTCGGCGGTCAACCTGCTCAAGG
ACAAGCAGTTGTTCATCGACGACACTGCCGGCCTCTCGCCCAACGAGATGCGGGCGCGGACGCGGCGTCTGGCACGCGAG
CACGGCAACATCGGGCTGATCATGATCGACTATCTTCAGCTCATGCAGATTCCGGGATTCTCCGAGAACCGCACCGGCGA
GATCTCGGAAATTTCGCGCTCGCTCAAGGCCCTGGCCAAGGAGTTCAATTGCCCGGTGGTGGCTCTCTCGCAGCTCAACC
GCTCGCTGGAGCAGCGTCCCAACAAGCGTCCGGTGATGTCCGACCTGCGCGAGTCGGGGGCCATCGAGCAGGATGCCGAC
CTCATTGCCTTCGTGTATCGCGACGAGGTCTATAATCCAGACAATCCCGACAACAAGGGGTTGGCCGAGTTGATCATCGG
CAAGCAGCGTAACGGGCCGATCGGTGCCGTGCACATGGCCTTCATCGGCAAGTACACCCGCTTCGAGGACCTCGCGCCGG
ATAGTTACGGCCAGCACATGGGCGAGTGA

Upstream 100 bases:

>100_bases
AGGGTGCAACGGCAATGACGTCGCGGCATCATATCAGTGGCCGAGACGGTGAGCGTATCCCGTTCGCCGGCCTTCGATAC
CGACCGGTCAGGAAACGAGC

Downstream 100 bases:

>100_bases
CGACGGCGCGGGCAAATGCACGGGGTATGCCTTATAATGCCCGACCCGCAAGTGAACAGGAGGGGAGATGGCGAGCGGAT
TTCAACGTGGCAAGAAGCGG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSEYVEHDQETAALKVPPHSLEAEQSVLGGLMLENSAWDTVSERLSADDFYRYEHRLIFNAMAQLADAGHPLDVVTLSEA
LENRDQLESVGGLAYLAELARNTPSASNIRAYTDIVRERATLRKLIQAAHQIADGAFAPQGRPADELVNEAERLVFQISE
DRPKTGGAIGMSDLLSKAVDRIDELFNMQGEMTGLSTGFRDLDEMTSGLQPSDLVIIAGRPSMGKTTFAMNVVEHAVISS
DKPVVVFSMEMPADALMLRMLSSLGRIDQTRVRTGQLEDEDWPRLTSAVNLLKDKQLFIDDTAGLSPNEMRARTRRLARE
HGNIGLIMIDYLQLMQIPGFSENRTGEISEISRSLKALAKEFNCPVVALSQLNRSLEQRPNKRPVMSDLRESGAIEQDAD
LIAFVYRDEVYNPDNPDNKGLAELIIGKQRNGPIGAVHMAFIGKYTRFEDLAPDSYGQHMGE

Sequences:

>Translated_462_residues
MSEYVEHDQETAALKVPPHSLEAEQSVLGGLMLENSAWDTVSERLSADDFYRYEHRLIFNAMAQLADAGHPLDVVTLSEA
LENRDQLESVGGLAYLAELARNTPSASNIRAYTDIVRERATLRKLIQAAHQIADGAFAPQGRPADELVNEAERLVFQISE
DRPKTGGAIGMSDLLSKAVDRIDELFNMQGEMTGLSTGFRDLDEMTSGLQPSDLVIIAGRPSMGKTTFAMNVVEHAVISS
DKPVVVFSMEMPADALMLRMLSSLGRIDQTRVRTGQLEDEDWPRLTSAVNLLKDKQLFIDDTAGLSPNEMRARTRRLARE
HGNIGLIMIDYLQLMQIPGFSENRTGEISEISRSLKALAKEFNCPVVALSQLNRSLEQRPNKRPVMSDLRESGAIEQDAD
LIAFVYRDEVYNPDNPDNKGLAELIIGKQRNGPIGAVHMAFIGKYTRFEDLAPDSYGQHMGE
>Mature_461_residues
SEYVEHDQETAALKVPPHSLEAEQSVLGGLMLENSAWDTVSERLSADDFYRYEHRLIFNAMAQLADAGHPLDVVTLSEAL
ENRDQLESVGGLAYLAELARNTPSASNIRAYTDIVRERATLRKLIQAAHQIADGAFAPQGRPADELVNEAERLVFQISED
RPKTGGAIGMSDLLSKAVDRIDELFNMQGEMTGLSTGFRDLDEMTSGLQPSDLVIIAGRPSMGKTTFAMNVVEHAVISSD
KPVVVFSMEMPADALMLRMLSSLGRIDQTRVRTGQLEDEDWPRLTSAVNLLKDKQLFIDDTAGLSPNEMRARTRRLAREH
GNIGLIMIDYLQLMQIPGFSENRTGEISEISRSLKALAKEFNCPVVALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADL
IAFVYRDEVYNPDNPDNKGLAELIIGKQRNGPIGAVHMAFIGKYTRFEDLAPDSYGQHMGE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=458, Percent_Identity=60.6986899563319, Blast_Score=575, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51234; Mature: 51102

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYVEHDQETAALKVPPHSLEAEQSVLGGLMLENSAWDTVSERLSADDFYRYEHRLIFN
CCCHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHH
AMAQLADAGHPLDVVTLSEALENRDQLESVGGLAYLAELARNTPSASNIRAYTDIVRERA
HHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
TLRKLIQAAHQIADGAFAPQGRPADELVNEAERLVFQISEDRPKTGGAIGMSDLLSKAVD
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
RIDELFNMQGEMTGLSTGFRDLDEMTSGLQPSDLVIIAGRPSMGKTTFAMNVVEHAVISS
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
DKPVVVFSMEMPADALMLRMLSSLGRIDQTRVRTGQLEDEDWPRLTSAVNLLKDKQLFID
CCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEE
DTAGLSPNEMRARTRRLAREHGNIGLIMIDYLQLMQIPGFSENRTGEISEISRSLKALAK
CCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
EFNCPVVALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADLIAFVYRDEVYNPDNPDNKG
HHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHEEEEEHHCCCCCCCCCCCC
LAELIIGKQRNGPIGAVHMAFIGKYTRFEDLAPDSYGQHMGE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure 
SEYVEHDQETAALKVPPHSLEAEQSVLGGLMLENSAWDTVSERLSADDFYRYEHRLIFN
CCHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHH
AMAQLADAGHPLDVVTLSEALENRDQLESVGGLAYLAELARNTPSASNIRAYTDIVRERA
HHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
TLRKLIQAAHQIADGAFAPQGRPADELVNEAERLVFQISEDRPKTGGAIGMSDLLSKAVD
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
RIDELFNMQGEMTGLSTGFRDLDEMTSGLQPSDLVIIAGRPSMGKTTFAMNVVEHAVISS
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
DKPVVVFSMEMPADALMLRMLSSLGRIDQTRVRTGQLEDEDWPRLTSAVNLLKDKQLFID
CCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEE
DTAGLSPNEMRARTRRLAREHGNIGLIMIDYLQLMQIPGFSENRTGEISEISRSLKALAK
CCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
EFNCPVVALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADLIAFVYRDEVYNPDNPDNKG
HHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHEEEEEHHCCCCCCCCCCCC
LAELIIGKQRNGPIGAVHMAFIGKYTRFEDLAPDSYGQHMGE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]