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The map label for this gene is yogA [H]

Identifier: 92112943

GI number: 92112943

Start: 910184

End: 911191

Strand: Direct

Name: yogA [H]

Synonym: Csal_0815

Alternate gene names: 92112943

Gene position: 910184-911191 (Clockwise)

Preceding gene: 92112941

Following gene: 92112944

Centisome position: 24.62

GC content: 67.86

Gene sequence:

>1008_bases
ATGAACGTCGTGACGTTACGTCGCCCCGGTGGGCTGGACAAGCTCGAGCTCCATGAAGGCGAGGCGCCGGGCGAGCCGGG
GCCCGGCGAGGTGCGTGTCCGCGTGCATGCCAGTTCGCTCAATTACCATGATTACGGCGTCGTCGCCGGGGCCATGCCCA
CCGAGGACGGTCGCATCCCCATGTCGGACGGTGCCGGTGTCGTCGACGCCGTGGGCGAGGGCGTCGAGGACTTCACCGTG
GGGGATCGCGTGGTATCGACGTTCTTCCCCGAGTGGCTCGAAGGGCCGGCACGGATCGGCGACTTCACCACCACGCCCGG
TGACGGTGTCGATGGCTATGCCCGCGAGCAGGTCGTGCGTCCCGCGACCTGGTTCACCCACGCGCCGCGCGATTACGATC
ATGCCGAGGCGGCCACGTTGACCACCGCCGGCTTGACCGCCTGGCGAGCGCTGGTGGTGGACGGCAAGCTCAAGGCCGGC
GATAGCGTGCTGGTGCTGGGGACCGGCGGCGTGTCGATCTTCGCGCTGCAGTTCGCCAAGCTGATGGGCGCCACGGTGAT
CGCGACCTCGTCCTCGGACGAGAAGATCGCACGGCTCAAGGAGTTGGGTGCCGATCACACGCTCAACTACAAGACCGAGC
CGGAGTGGGGCAAGCGCGTCAAGGCACTCACCGATGGCCGGGGCGTGGACCACGTCATCGAGGTCGGAGGGCCGGCCACG
CTGCCGCAGTCCATCGAGGCCGTGCGCATTGGCGGTCACATCTCGTTGATCGGCGTGCTCACCGGACGCGGCGGCGAAAT
TCCTACCGCCAAGCTGATGGCCAAGCAGGCGCGTCTGCAGGGCTTGATCGTGGGCAGCCGGCGCGACCAGATCGAGATGA
TCCGCGCCATCGACGCCAGCGGCATGCGGCCGGTCATCGACCGCCGGTTCGCCCTCGACGAGATCGCCGACGCGTTTCGT
CACCAGGAGGCGGGGCGTCACTTCGGCAAGATCTGCCTGGAGTACTGA

Upstream 100 bases:

>100_bases
GCCTCGCGACCAATCGTCACCATCATGAAGGAAGGACATTCACGATGTCGCGCCATGGCGTGGCCTCGTGGTAACGCTGG
ATGACCGTAAGGAGAGTGCA

Downstream 100 bases:

>100_bases
GCGGCAGGATGGCTTACACCGACGCGAACGGCGACGTGATCGACCATCGTCGCCATCCGGAGTGCTATCGCATCGGACGC
GGGGAGCAGGGCGTGCTCAA

Product: zinc-binding alcohol dehydrogenase

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNVVTLRRPGGLDKLELHEGEAPGEPGPGEVRVRVHASSLNYHDYGVVAGAMPTEDGRIPMSDGAGVVDAVGEGVEDFTV
GDRVVSTFFPEWLEGPARIGDFTTTPGDGVDGYAREQVVRPATWFTHAPRDYDHAEAATLTTAGLTAWRALVVDGKLKAG
DSVLVLGTGGVSIFALQFAKLMGATVIATSSSDEKIARLKELGADHTLNYKTEPEWGKRVKALTDGRGVDHVIEVGGPAT
LPQSIEAVRIGGHISLIGVLTGRGGEIPTAKLMAKQARLQGLIVGSRRDQIEMIRAIDASGMRPVIDRRFALDEIADAFR
HQEAGRHFGKICLEY

Sequences:

>Translated_335_residues
MNVVTLRRPGGLDKLELHEGEAPGEPGPGEVRVRVHASSLNYHDYGVVAGAMPTEDGRIPMSDGAGVVDAVGEGVEDFTV
GDRVVSTFFPEWLEGPARIGDFTTTPGDGVDGYAREQVVRPATWFTHAPRDYDHAEAATLTTAGLTAWRALVVDGKLKAG
DSVLVLGTGGVSIFALQFAKLMGATVIATSSSDEKIARLKELGADHTLNYKTEPEWGKRVKALTDGRGVDHVIEVGGPAT
LPQSIEAVRIGGHISLIGVLTGRGGEIPTAKLMAKQARLQGLIVGSRRDQIEMIRAIDASGMRPVIDRRFALDEIADAFR
HQEAGRHFGKICLEY
>Mature_335_residues
MNVVTLRRPGGLDKLELHEGEAPGEPGPGEVRVRVHASSLNYHDYGVVAGAMPTEDGRIPMSDGAGVVDAVGEGVEDFTV
GDRVVSTFFPEWLEGPARIGDFTTTPGDGVDGYAREQVVRPATWFTHAPRDYDHAEAATLTTAGLTAWRALVVDGKLKAG
DSVLVLGTGGVSIFALQFAKLMGATVIATSSSDEKIARLKELGADHTLNYKTEPEWGKRVKALTDGRGVDHVIEVGGPAT
LPQSIEAVRIGGHISLIGVLTGRGGEIPTAKLMAKQARLQGLIVGSRRDQIEMIRAIDASGMRPVIDRRFALDEIADAFR
HQEAGRHFGKICLEY

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=354, Percent_Identity=26.8361581920904, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI22538444, Length=354, Percent_Identity=26.8361581920904, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI194239674, Length=328, Percent_Identity=27.7439024390244, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI13236495, Length=328, Percent_Identity=27.7439024390244, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI28557745, Length=245, Percent_Identity=32.6530612244898, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI47519420, Length=351, Percent_Identity=26.2108262108262, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI156627571, Length=325, Percent_Identity=27.0769230769231, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI18379349, Length=356, Percent_Identity=25.8426966292135, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI194239676, Length=327, Percent_Identity=25.6880733944954, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI24308257, Length=376, Percent_Identity=24.7340425531915, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI87081918, Length=315, Percent_Identity=27.6190476190476, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788075, Length=323, Percent_Identity=26.9349845201238, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788073, Length=211, Percent_Identity=31.2796208530806, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790485, Length=263, Percent_Identity=26.2357414448669, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=336, Percent_Identity=27.3809523809524, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17507255, Length=333, Percent_Identity=27.027027027027, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17562878, Length=339, Percent_Identity=25.6637168141593, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323729, Length=339, Percent_Identity=25.9587020648968, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=336, Percent_Identity=25.5952380952381, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323099, Length=346, Percent_Identity=24.5664739884393, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324486, Length=337, Percent_Identity=25.5192878338279, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=337, Percent_Identity=26.4094955489614, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319955, Length=283, Percent_Identity=24.7349823321555, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322619, Length=283, Percent_Identity=24.7349823321555, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581345, Length=220, Percent_Identity=25.9090909090909, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920632, Length=220, Percent_Identity=25.9090909090909, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI221330659, Length=220, Percent_Identity=25.9090909090909, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 35809; Mature: 35809

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVTLRRPGGLDKLELHEGEAPGEPGPGEVRVRVHASSLNYHDYGVVAGAMPTEDGRIP
CCEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCEE
MSDGAGVVDAVGEGVEDFTVGDRVVSTFFPEWLEGPARIGDFTTTPGDGVDGYAREQVVR
CCCCCCHHHHHCCCCHHHHHHHHHHHHHCHHHHCCCHHCCCCCCCCCCCCCCHHHHHHCC
PATWFTHAPRDYDHAEAATLTTAGLTAWRALVVDGKLKAGDSVLVLGTGGVSIFALQFAK
CHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCHHHHHHHHHH
LMGATVIATSSSDEKIARLKELGADHTLNYKTEPEWGKRVKALTDGRGVDHVIEVGGPAT
HHCCEEEEECCCHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCC
LPQSIEAVRIGGHISLIGVLTGRGGEIPTAKLMAKQARLQGLIVGSRRDQIEMIRAIDAS
CCCCCEEEEECCEEEEEEEEECCCCCCCHHHHHHHHHHHCEEEECCCCHHHHHHHHHCCC
GMRPVIDRRFALDEIADAFRHQEAGRHFGKICLEY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNVVTLRRPGGLDKLELHEGEAPGEPGPGEVRVRVHASSLNYHDYGVVAGAMPTEDGRIP
CCEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCEE
MSDGAGVVDAVGEGVEDFTVGDRVVSTFFPEWLEGPARIGDFTTTPGDGVDGYAREQVVR
CCCCCCHHHHHCCCCHHHHHHHHHHHHHCHHHHCCCHHCCCCCCCCCCCCCCHHHHHHCC
PATWFTHAPRDYDHAEAATLTTAGLTAWRALVVDGKLKAGDSVLVLGTGGVSIFALQFAK
CHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCHHHHHHHHHH
LMGATVIATSSSDEKIARLKELGADHTLNYKTEPEWGKRVKALTDGRGVDHVIEVGGPAT
HHCCEEEEECCCHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCC
LPQSIEAVRIGGHISLIGVLTGRGGEIPTAKLMAKQARLQGLIVGSRRDQIEMIRAIDAS
CCCCCEEEEECCEEEEEEEEECCCCCCCHHHHHHHHHHHCEEEECCCCHHHHHHHHHCCC
GMRPVIDRRFALDEIADAFRHQEAGRHFGKICLEY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.005 {cyclohexanol}} 0.004 {

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]