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The map label for this gene is ywlF [H]

Identifier: 92112886

GI number: 92112886

Start: 851025

End: 851471

Strand: Direct

Name: ywlF [H]

Synonym: Csal_0757

Alternate gene names: 92112886

Gene position: 851025-851471 (Clockwise)

Preceding gene: 92112885

Following gene: 92112887

Centisome position: 23.02

GC content: 61.74

Gene sequence:

>447_bases
ATGAAGATCGTCATCGGCTCGGATCATGCGGGCTACAGGCTCAAGGCCTCGGTGGTGGAATACGTGGAATCGCTCGGCCA
TGAAGTGATCGACGTGGGGTCGCACGATGACACCCCGGTGGATTTCCCCGATATTGCGAAGCAGATGGCCGGCAAGGTGA
CGAGTGGCGAGGCGGCCCGGGGCTTGATGGTCTGCGGTACCGGCGTGGGCGCCTCCATTGCCGCCAACAAGATTCGCGGC
ATTCGCGCCGCGGTGTGCCATGACATCCATTCGGCGCATCAGTGCGTCGAGCACGATGACGTCAACGTGATGTGCATCGG
CGCGCAGATCGTCGGCCCCTGGCTGGCCAACGACCTGATCAAGGCCTATCTCGAAGCCGAGTTTTCCACCAGCGAAGAAT
TCCGCCGGCGCGTGGAAAAGCTGCATGCCATGGAGAACGATGGCTAG

Upstream 100 bases:

>100_bases
ATGTGCCGCTGCTGACGACGCTGGCCATTATCACGTTTGCCTCGGACCTGGTGCTCTGGTTGCCCGGCATGCTTCGCTAA
ACAAAACCTGGAGTGGATAT

Downstream 100 bases:

>100_bases
CACCGCAATCGAGAAGTTACCCAGGCCGCCGAGCGCCGGTCAGCGCTGGCGGCCACGAAATCCAACCAACCATCCAGCCC
CTGCCCTGAAGCCGAACGCG

Product: ribose-5-phosphate isomerase B

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MKIVIGSDHAGYRLKASVVEYVESLGHEVIDVGSHDDTPVDFPDIAKQMAGKVTSGEAARGLMVCGTGVGASIAANKIRG
IRAAVCHDIHSAHQCVEHDDVNVMCIGAQIVGPWLANDLIKAYLEAEFSTSEEFRRRVEKLHAMENDG

Sequences:

>Translated_148_residues
MKIVIGSDHAGYRLKASVVEYVESLGHEVIDVGSHDDTPVDFPDIAKQMAGKVTSGEAARGLMVCGTGVGASIAANKIRG
IRAAVCHDIHSAHQCVEHDDVNVMCIGAQIVGPWLANDLIKAYLEAEFSTSEEFRRRVEKLHAMENDG
>Mature_148_residues
MKIVIGSDHAGYRLKASVVEYVESLGHEVIDVGSHDDTPVDFPDIAKQMAGKVTSGEAARGLMVCGTGVGASIAANKIRG
IRAAVCHDIHSAHQCVEHDDVNVMCIGAQIVGPWLANDLIKAYLEAEFSTSEEFRRRVEKLHAMENDG

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=145, Percent_Identity=42.0689655172414, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15928; Mature: 15928

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIGSDHAGYRLKASVVEYVESLGHEVIDVGSHDDTPVDFPDIAKQMAGKVTSGEAAR
CEEEECCCCCCCCHHHHHHHHHHHHCHHEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCC
GLMVCGTGVGASIAANKIRGIRAAVCHDIHSAHQCVEHDDVNVMCIGAQIVGPWLANDLI
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHH
KAYLEAEFSTSEEFRRRVEKLHAMENDG
HHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIVIGSDHAGYRLKASVVEYVESLGHEVIDVGSHDDTPVDFPDIAKQMAGKVTSGEAAR
CEEEECCCCCCCCHHHHHHHHHHHHCHHEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCC
GLMVCGTGVGASIAANKIRGIRAAVCHDIHSAHQCVEHDDVNVMCIGAQIVGPWLANDLI
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHH
KAYLEAEFSTSEEFRRRVEKLHAMENDG
HHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]