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The map label for this gene is yfhA [H]

Identifier: 92112849

GI number: 92112849

Start: 804725

End: 806125

Strand: Direct

Name: yfhA [H]

Synonym: Csal_0717

Alternate gene names: 92112849

Gene position: 804725-806125 (Clockwise)

Preceding gene: 92112848

Following gene: 92112855

Centisome position: 21.77

GC content: 67.74

Gene sequence:

>1401_bases
GTGAAGCACGCCGCCGCGCATATTCTGCTGGTCGATGACGACCCCAGCCTGCTCAAGTTGCTGGGCATGCGCCTGGAAAG
CCGAGGCTACCGCGTGACCACCGCCGAGAGTGGACGCCAGGCACTGGACTGCCTGACCGAGGACCGCCCCGACCTGGTGC
TCTCCGACCTGCGCATGGACGAAATGGACGGCATGGCACTGTTTCACGAATTGCAGCGCCGCGCGCCGGGCATGCCGGTG
ATCATTCTCACCGCGCACGGCTCCATCCCGGATGCGGTGAGCGCCACGCGCCAGGGGGTGTTCAGCTTTCTCACCAAGCC
GGTGGACCGCGACGAGCTGTTCGCCGCCATCGAGGAAGCCATGGCGCAGACGGCGAGCACCCACGGGGCCGGCGACGATG
CCTGGCGTGCCGAGATCATCACCCGCAGCCCGCAGATGGAACGCATCCTCGAGCAGGCCCGCATGGTGGCGGCTTCGGAT
GTCAGCGTGCTGATCACCGGGGCCTCCGGCTCGGGCAAGGAGCTGCTGGCCAACGCCATCCACCAGGCGAGCGGACGCGC
CGACAAGCCCTTCGTGGCGATCAACTGCGGCGCGCTGCCCGAGCAGTTGCTGGAAAGCGAGCTGTTCGGGCACGCCAAGG
GCGCCTTCACCGGTGCGGTGAGCCAGCACGAGGGGCTGTTCCTCGCCGCCAACGGCGGCAGCCTGTTCCTCGACGAGATC
GGCGACATGCCGCTGACGCTGCAGGTCAAATTGTTGCGCGCGCTGCAGGAACGTCAGATTCGCCCCCTGGGCTCGACCAC
TTCGGTGCCCATCGACGTGCGCATCATCTCCGCCACGCACCGCGACCTCGACCGCGCCATGCACGAGGGCGACTTCCGTG
AGGACCTTTATTACCGCCTCAACGTCGTCAACCTCAAGCTGCCGGCCCTCAAGGAGCGTGCCGAGGACGTGCCGTTGCTG
GCCAAGTATCTGGTGGGACAGGCGGCAGAGCGCCACAAGCCCTTCGTCAAGGGCTTCTCGTCGGAGGCGCTCAACCTGCT
GGCCTCCAGCGCCTGGCCGGGCAACGTGCGCCAGCTGGTCAACGTGGTCGAGCAGTGCGTGGCGCTGACCAGCTCGCCGA
TCATCCCCGAGGCCCTGGTGGCCCAGGCGCTGGCGGCCGAGGAAAACGCGCTGCCCTCGTTCAACGAGGCGCGCGCGGGC
TTCGAACGCAGCTATCTCGTCAAGGTGCTGAAGATCACCGAGGGCAACGTTACCCAGGCCGCGCGCATCGCCGGGCGCAA
CCGCACCGACTTCTACAAGCTGCTGGCGCGCCACGACCTGGAACCGAGCAGCTTCAAGCCGAGCACCCACTCGCAGTCGG
CACAGAACGACGACGCCGCACCACGCCGCGCCCAGCGCTGA

Upstream 100 bases:

>100_bases
CTCGCCGAGAAACTCGACGCCCTGACGGCCATCGAACAGAGCATCAACTCGCGTCATCAGCAATCGCCATGACGGCGCGA
ATGAGCCCAAGGAGAAAGAC

Downstream 100 bases:

>100_bases
ATTCGTTCAGAGATAGGTGTCAGACAGTGCCTAACGCTTGAAGCGCCGCTTCACCTCCACCTTCAACCGCCCCTCGCCGA
GTCGCGCGGTGAGGGTCTGG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKHAAAHILLVDDDPSLLKLLGMRLESRGYRVTTAESGRQALDCLTEDRPDLVLSDLRMDEMDGMALFHELQRRAPGMPV
IILTAHGSIPDAVSATRQGVFSFLTKPVDRDELFAAIEEAMAQTASTHGAGDDAWRAEIITRSPQMERILEQARMVAASD
VSVLITGASGSGKELLANAIHQASGRADKPFVAINCGALPEQLLESELFGHAKGAFTGAVSQHEGLFLAANGGSLFLDEI
GDMPLTLQVKLLRALQERQIRPLGSTTSVPIDVRIISATHRDLDRAMHEGDFREDLYYRLNVVNLKLPALKERAEDVPLL
AKYLVGQAAERHKPFVKGFSSEALNLLASSAWPGNVRQLVNVVEQCVALTSSPIIPEALVAQALAAEENALPSFNEARAG
FERSYLVKVLKITEGNVTQAARIAGRNRTDFYKLLARHDLEPSSFKPSTHSQSAQNDDAAPRRAQR

Sequences:

>Translated_466_residues
MKHAAAHILLVDDDPSLLKLLGMRLESRGYRVTTAESGRQALDCLTEDRPDLVLSDLRMDEMDGMALFHELQRRAPGMPV
IILTAHGSIPDAVSATRQGVFSFLTKPVDRDELFAAIEEAMAQTASTHGAGDDAWRAEIITRSPQMERILEQARMVAASD
VSVLITGASGSGKELLANAIHQASGRADKPFVAINCGALPEQLLESELFGHAKGAFTGAVSQHEGLFLAANGGSLFLDEI
GDMPLTLQVKLLRALQERQIRPLGSTTSVPIDVRIISATHRDLDRAMHEGDFREDLYYRLNVVNLKLPALKERAEDVPLL
AKYLVGQAAERHKPFVKGFSSEALNLLASSAWPGNVRQLVNVVEQCVALTSSPIIPEALVAQALAAEENALPSFNEARAG
FERSYLVKVLKITEGNVTQAARIAGRNRTDFYKLLARHDLEPSSFKPSTHSQSAQNDDAAPRRAQR
>Mature_466_residues
MKHAAAHILLVDDDPSLLKLLGMRLESRGYRVTTAESGRQALDCLTEDRPDLVLSDLRMDEMDGMALFHELQRRAPGMPV
IILTAHGSIPDAVSATRQGVFSFLTKPVDRDELFAAIEEAMAQTASTHGAGDDAWRAEIITRSPQMERILEQARMVAASD
VSVLITGASGSGKELLANAIHQASGRADKPFVAINCGALPEQLLESELFGHAKGAFTGAVSQHEGLFLAANGGSLFLDEI
GDMPLTLQVKLLRALQERQIRPLGSTTSVPIDVRIISATHRDLDRAMHEGDFREDLYYRLNVVNLKLPALKERAEDVPLL
AKYLVGQAAERHKPFVKGFSSEALNLLASSAWPGNVRQLVNVVEQCVALTSSPIIPEALVAQALAAEENALPSFNEARAG
FERSYLVKVLKITEGNVTQAARIAGRNRTDFYKLLARHDLEPSSFKPSTHSQSAQNDDAAPRRAQR

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=446, Percent_Identity=69.9551569506726, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=45.578231292517, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=40.9190371991247, Blast_Score=344, Evalue=7e-96,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=40.0862068965517, Blast_Score=324, Evalue=8e-90,
Organism=Escherichia coli, GI87082117, Length=335, Percent_Identity=45.9701492537313, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1789087, Length=333, Percent_Identity=44.4444444444444, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1789233, Length=240, Percent_Identity=48.75, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=40.625, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1786524, Length=337, Percent_Identity=39.7626112759644, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1787583, Length=298, Percent_Identity=38.5906040268456, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081872, Length=311, Percent_Identity=37.620578778135, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1789828, Length=257, Percent_Identity=39.2996108949416, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=29.903536977492, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693157, Length=104, Percent_Identity=38.4615384615385, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50788; Mature: 50788

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAAAHILLVDDDPSLLKLLGMRLESRGYRVTTAESGRQALDCLTEDRPDLVLSDLRMD
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCHHHHHCCHH
EMDGMALFHELQRRAPGMPVIILTAHGSIPDAVSATRQGVFSFLTKPVDRDELFAAIEEA
HCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MAQTASTHGAGDDAWRAEIITRSPQMERILEQARMVAASDVSVLITGASGSGKELLANAI
HHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
HQASGRADKPFVAINCGALPEQLLESELFGHAKGAFTGAVSQHEGLFLAANGGSLFLDEI
HHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEECCCEEEHHHH
GDMPLTLQVKLLRALQERQIRPLGSTTSVPIDVRIISATHRDLDRAMHEGDFREDLYYRL
CCCCEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCCCHHHHEEEE
NVVNLKLPALKERAEDVPLLAKYLVGQAAERHKPFVKGFSSEALNLLASSAWPGNVRQLV
EEEEEECHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCHHHHHHH
NVVEQCVALTSSPIIPEALVAQALAAEENALPSFNEARAGFERSYLVKVLKITEGNVTQA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHCCCCCHHHH
ARIAGRNRTDFYKLLARHDLEPSSFKPSTHSQSAQNDDAAPRRAQR
HHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKHAAAHILLVDDDPSLLKLLGMRLESRGYRVTTAESGRQALDCLTEDRPDLVLSDLRMD
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCHHHHHCCHH
EMDGMALFHELQRRAPGMPVIILTAHGSIPDAVSATRQGVFSFLTKPVDRDELFAAIEEA
HCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MAQTASTHGAGDDAWRAEIITRSPQMERILEQARMVAASDVSVLITGASGSGKELLANAI
HHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
HQASGRADKPFVAINCGALPEQLLESELFGHAKGAFTGAVSQHEGLFLAANGGSLFLDEI
HHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEECCCEEEHHHH
GDMPLTLQVKLLRALQERQIRPLGSTTSVPIDVRIISATHRDLDRAMHEGDFREDLYYRL
CCCCEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCCCHHHHEEEE
NVVNLKLPALKERAEDVPLLAKYLVGQAAERHKPFVKGFSSEALNLLASSAWPGNVRQLV
EEEEEECHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCHHHHHHH
NVVEQCVALTSSPIIPEALVAQALAAEENALPSFNEARAGFERSYLVKVLKITEGNVTQA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHCCCCCHHHH
ARIAGRNRTDFYKLLARHDLEPSSFKPSTHSQSAQNDDAAPRRAQR
HHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]