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The map label for this gene is gloA [H]

Identifier: 92112817

GI number: 92112817

Start: 768932

End: 769372

Strand: Direct

Name: gloA [H]

Synonym: Csal_0685

Alternate gene names: 92112817

Gene position: 768932-769372 (Clockwise)

Preceding gene: 92112814

Following gene: 92112818

Centisome position: 20.8

GC content: 61.22

Gene sequence:

>441_bases
ATGCAGTACCTGCATACGATGGTTCGTGTCGCCGATCTCGACGCGTCGCTGCATTTTTATTGCGACCTGCTGGGCCTCAA
GGAGGTTCGCCGCAAGGAGAACGAAAAGGGCCGCTTCACGCTGGTGTTCCTTGCCGCGCCCGACGACGAGCAGCGCTCGC
AAGAGTTGAAGGCCCCCGAGCTGGAGCTGACCTACAACTGGGACCCCGAGACCTATACGGGCGGACGCAACTTCGGCCAC
CTGGCATATCGGGTCGAGGATATCTATGCGCTTTGCCAGCACTTGATGGACAACGGCGTGACCATCAATCGGCCCCCGCG
CGACGGTCACATGGCCTTCGTCCGCTCGCCGGACGGCATCTCCGTGGAGTTGCTGCAAAAAGGCGATGCGCTGCCCGTAC
AGGAGCCGTGGGCCTCGATGGAAAATACCGGAAGCTGGTAA

Upstream 100 bases:

>100_bases
AAGCATGAACAAAGCGGTCCGGGCGGTCCAGCGTTCGCGGCAATCGCCGGGCTGGTTTACCATTGACGTTTAGCCTGCAT
AACATCAGGGGGAGATTACC

Downstream 100 bases:

>100_bases
GCGAGCCATGCCGCGCCCCGCGAGGAAACGATGCCGGGCGCGGCATCATCGATATTGCCTGCAAGGCGAGAAAGGAATCT
CCGCATGTCGCAAGACGTAA

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MQYLHTMVRVADLDASLHFYCDLLGLKEVRRKENEKGRFTLVFLAAPDDEQRSQELKAPELELTYNWDPETYTGGRNFGH
LAYRVEDIYALCQHLMDNGVTINRPPRDGHMAFVRSPDGISVELLQKGDALPVQEPWASMENTGSW

Sequences:

>Translated_146_residues
MQYLHTMVRVADLDASLHFYCDLLGLKEVRRKENEKGRFTLVFLAAPDDEQRSQELKAPELELTYNWDPETYTGGRNFGH
LAYRVEDIYALCQHLMDNGVTINRPPRDGHMAFVRSPDGISVELLQKGDALPVQEPWASMENTGSW
>Mature_146_residues
MQYLHTMVRVADLDASLHFYCDLLGLKEVRRKENEKGRFTLVFLAAPDDEQRSQELKAPELELTYNWDPETYTGGRNFGH
LAYRVEDIYALCQHLMDNGVTINRPPRDGHMAFVRSPDGISVELLQKGDALPVQEPWASMENTGSW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=34.965034965035, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI1787940, Length=136, Percent_Identity=36.7647058823529, Blast_Score=92, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323639, Length=141, Percent_Identity=32.6241134751773, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=34.7222222222222, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16755; Mature: 16755

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYLHTMVRVADLDASLHFYCDLLGLKEVRRKENEKGRFTLVFLAAPDDEQRSQELKAPE
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCC
LELTYNWDPETYTGGRNFGHLAYRVEDIYALCQHLMDNGVTINRPPRDGHMAFVRSPDGI
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCC
SVELLQKGDALPVQEPWASMENTGSW
EEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQYLHTMVRVADLDASLHFYCDLLGLKEVRRKENEKGRFTLVFLAAPDDEQRSQELKAPE
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCC
LELTYNWDPETYTGGRNFGHLAYRVEDIYALCQHLMDNGVTINRPPRDGHMAFVRSPDGI
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCC
SVELLQKGDALPVQEPWASMENTGSW
EEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]