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The map label for this gene is yieH [H]

Identifier: 92112770

GI number: 92112770

Start: 714870

End: 715562

Strand: Direct

Name: yieH [H]

Synonym: Csal_0637

Alternate gene names: 92112770

Gene position: 714870-715562 (Clockwise)

Preceding gene: 92112769

Following gene: 92112771

Centisome position: 19.34

GC content: 69.84

Gene sequence:

>693_bases
ATGAAGTCGCCCATGGAGGTGCTGATCTTCGACTGCGACGGCGTGCTGGTGGACAGCGAAGCCATCGCCGAGACGGTGCT
GCGCGAGCGACTGGCGGCCTGGCTGCCTGACCTGGCGATCGGTGATGAATTGCGCCGGGCACTGGGCATGACTACCCAGG
CGATTCTCGATCATCTGGCTGCCCGCAGCGCGCATGCCTTGCCCGATGACGCCCTCGAACGCATCGACACGGCGATCGAG
GCGCGATTGGCCGAGGAGCTCAAGGCCATGGCCGGCGTGGCGGCTGCCATCGAGGCCCTCGATCTGCCGCTGGCGATCGT
CTCCAACAGCCGCCGCCGTCGCGTCGTCGCCTCGCTGGCCAACACCGGGCTCGATGCGCAACTCGGCAAGGCGCCGTTGT
TCACCGCCGAGCAGGTCGCGCACCCCAAGCCGGCGCCGGATGTCTACCGCCTGGCGGCGTCGCATCTCGGTGTCGCGCCT
GCCGCCTGCCTCGTCATCGAGGACAGTGTCTCGGGGACCCGGGCGGCCTGTGCCGCCGGCATGACGGTGATCGGTTTCAC
CGGCGCCAGTCATGTCGAGCCCGGCCATGCACAACGGCTGCGCGACGCCGGCGCCTGGCAGGTGCTCACGCACATGGCGG
CGCTTGGCGAGCTGGTACAGCGCTGGCGCGGCGAGCGTCACGCCGTGCGCTGA

Upstream 100 bases:

>100_bases
TCACTGCCGATGGCCTGGCCCTGGCGCACCTGACGCGCCACCCGCTGGCCGATATCGACCGCAAGGAAAATCGCGCCTCT
TCGGCATCGGACGGCCGCTG

Downstream 100 bases:

>100_bases
AGACCGCCGGGGGGATATGGCCGACGGCCCGAAGCTGGCGCTTCATCATGACTTCACTCGAACAAGGAACCACAGCGATG
AAACTCGACAAGCGAACGGC

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKSPMEVLIFDCDGVLVDSEAIAETVLRERLAAWLPDLAIGDELRRALGMTTQAILDHLAARSAHALPDDALERIDTAIE
ARLAEELKAMAGVAAAIEALDLPLAIVSNSRRRRVVASLANTGLDAQLGKAPLFTAEQVAHPKPAPDVYRLAASHLGVAP
AACLVIEDSVSGTRAACAAGMTVIGFTGASHVEPGHAQRLRDAGAWQVLTHMAALGELVQRWRGERHAVR

Sequences:

>Translated_230_residues
MKSPMEVLIFDCDGVLVDSEAIAETVLRERLAAWLPDLAIGDELRRALGMTTQAILDHLAARSAHALPDDALERIDTAIE
ARLAEELKAMAGVAAAIEALDLPLAIVSNSRRRRVVASLANTGLDAQLGKAPLFTAEQVAHPKPAPDVYRLAASHLGVAP
AACLVIEDSVSGTRAACAAGMTVIGFTGASHVEPGHAQRLRDAGAWQVLTHMAALGELVQRWRGERHAVR
>Mature_230_residues
MKSPMEVLIFDCDGVLVDSEAIAETVLRERLAAWLPDLAIGDELRRALGMTTQAILDHLAARSAHALPDDALERIDTAIE
ARLAEELKAMAGVAAAIEALDLPLAIVSNSRRRRVVASLANTGLDAQLGKAPLFTAEQVAHPKPAPDVYRLAASHLGVAP
AACLVIEDSVSGTRAACAAGMTVIGFTGASHVEPGHAQRLRDAGAWQVLTHMAALGELVQRWRGERHAVR

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=224, Percent_Identity=28.125, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788021, Length=192, Percent_Identity=31.7708333333333, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24392; Mature: 24392

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPMEVLIFDCDGVLVDSEAIAETVLRERLAAWLPDLAIGDELRRALGMTTQAILDHLA
CCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHH
ARSAHALPDDALERIDTAIEARLAEELKAMAGVAAAIEALDLPLAIVSNSRRRRVVASLA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHH
NTGLDAQLGKAPLFTAEQVAHPKPAPDVYRLAASHLGVAPAACLVIEDSVSGTRAACAAG
HCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCHHHEEEEECCCCCHHHHHHHC
MTVIGFTGASHVEPGHAQRLRDAGAWQVLTHMAALGELVQRWRGERHAVR
CCEEEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSPMEVLIFDCDGVLVDSEAIAETVLRERLAAWLPDLAIGDELRRALGMTTQAILDHLA
CCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHH
ARSAHALPDDALERIDTAIEARLAEELKAMAGVAAAIEALDLPLAIVSNSRRRRVVASLA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHH
NTGLDAQLGKAPLFTAEQVAHPKPAPDVYRLAASHLGVAPAACLVIEDSVSGTRAACAAG
HCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCHHHEEEEECCCCCHHHHHHHC
MTVIGFTGASHVEPGHAQRLRDAGAWQVLTHMAALGELVQRWRGERHAVR
CCEEEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]