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The map label for this gene is rho [H]

Identifier: 92112717

GI number: 92112717

Start: 648610

End: 649869

Strand: Direct

Name: rho [H]

Synonym: Csal_0584

Alternate gene names: 92112717

Gene position: 648610-649869 (Clockwise)

Preceding gene: 92112716

Following gene: 92112718

Centisome position: 17.55

GC content: 60.95

Gene sequence:

>1260_bases
ATGAATCTTACCGAACTCAAGCAAAAAACCGTTCCCGAACTGCTGGATATTGCCCGGGAAATGGGCATCGACAATCTGGC
GCGTTCGCGTAAACAAGACATCATCTTTGCCATTCTCAAGAAGCACGCGAAAAGTGGCGAAGATATCTATGGCGATGGGG
TGCTGGAAATCCTGCAGGACGGCTTCGGCTTTCTGCGCAGTGCCGATAGCTCCTATCTGGCCGGCCCCGACGATATCTAT
GTCTCGCCGTCGCAGATTCGCCGTTTCAACCTGCGCAAGGGCGACAGCATCGCCGGCAAGATCCGGCCGCCGAAGGAAGG
GGAGCGCTATTTCGCGCTGCTCAAGGTCAACGAGATCAACTTCGATCGGCCGGAAAACGCCAAGCACAAGATCCTGTTCG
AGAACCTGACGCCGCTGTTCCCTCAGGAACGCATGGTGATGGAGATCGGCAACGGTTCGACCGAGGATCTGACCTCGCGG
GTGATCGACCTCACATCGCCCATCGGCAAGGGCCAGCGTGGCCTGATCGTCTCGCCCCCCAAGGCGGGCAAGACGCTGAT
GCTGCAGAACATCGCCACGTCCATCACGCGCAACAACCCCGAGTGTCACCTGATCGTGCTGCTGATCGACGAGCGGCCCG
AGGAGGTCACCGAGATGTCGCGCACCGTGCGTGGCGAAGTGGTGGCCTCGACCTTCGACGAGCCGCCCGCGCGTCACGTT
CAGGTCGCCGAGATGGTCATCGAGAAAGCCAAGCGCCTGGTCGAGCACAAGCGCGACGTGGTGGTGCTGCTCGATTCCAT
CACGCGCCTGGCGCGCGCCTACAACACCGTGGTGCCGTCGTCCGGCAAGGTGCTGACCGGCGGTGTCGACGCGCATGCGC
TCGAGAAGCCCAAGCGTTTCTTCGGGGCCGCGCGCAACATCGAGGAAGGCGGCAGCCTGACCATCATCGCCACGGCGCTG
GTGGACACCGGTTCCAAGATGGACGAGGTCATCTTCGAGGAGTTCAAGGGGACCGGCAACATGGAAGCGCACCTCGACCG
CAAGCTGGCCGAGAAGCGCGTCTATCCGGCGCTCAACATCCGCCGCTCCGGCACGCGTCGCGAGGACCTCATCGCCTCCG
AGGAAGAAATGCAGCGCATGTGGATCCTGCGCAAGCTGCTCAATCCGATGGACGACGTGGCGGCGACGGAGTTTCTGATC
GATCGTCTGAAGGATACCAAGACGAATATCGAATTCTTCGAGGCCATGAAAAGGCGTTGA

Upstream 100 bases:

>100_bases
CTGGGGCCCTCTGAACGCACCGCGCGGCGCACAACCGCCTCCTGTCTTTTGTCCGGCAATCCCATTGCCTCGCCGACACG
AGCAATCTCTGACGAAACCG

Downstream 100 bases:

>100_bases
TTCTGGCCTGCTGCGCTCGGCAACACGGCGTTGAACTCCGGTTCGCCATGCTCATGTACTAAAGTACACCCCGCTGGCTC
GCCGGAGTTCGCCTTGTTTT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNLTELKQKTVPELLDIAREMGIDNLARSRKQDIIFAILKKHAKSGEDIYGDGVLEILQDGFGFLRSADSSYLAGPDDIY
VSPSQIRRFNLRKGDSIAGKIRPPKEGERYFALLKVNEINFDRPENAKHKILFENLTPLFPQERMVMEIGNGSTEDLTSR
VIDLTSPIGKGQRGLIVSPPKAGKTLMLQNIATSITRNNPECHLIVLLIDERPEEVTEMSRTVRGEVVASTFDEPPARHV
QVAEMVIEKAKRLVEHKRDVVVLLDSITRLARAYNTVVPSSGKVLTGGVDAHALEKPKRFFGAARNIEEGGSLTIIATAL
VDTGSKMDEVIFEEFKGTGNMEAHLDRKLAEKRVYPALNIRRSGTRREDLIASEEEMQRMWILRKLLNPMDDVAATEFLI
DRLKDTKTNIEFFEAMKRR

Sequences:

>Translated_419_residues
MNLTELKQKTVPELLDIAREMGIDNLARSRKQDIIFAILKKHAKSGEDIYGDGVLEILQDGFGFLRSADSSYLAGPDDIY
VSPSQIRRFNLRKGDSIAGKIRPPKEGERYFALLKVNEINFDRPENAKHKILFENLTPLFPQERMVMEIGNGSTEDLTSR
VIDLTSPIGKGQRGLIVSPPKAGKTLMLQNIATSITRNNPECHLIVLLIDERPEEVTEMSRTVRGEVVASTFDEPPARHV
QVAEMVIEKAKRLVEHKRDVVVLLDSITRLARAYNTVVPSSGKVLTGGVDAHALEKPKRFFGAARNIEEGGSLTIIATAL
VDTGSKMDEVIFEEFKGTGNMEAHLDRKLAEKRVYPALNIRRSGTRREDLIASEEEMQRMWILRKLLNPMDDVAATEFLI
DRLKDTKTNIEFFEAMKRR
>Mature_419_residues
MNLTELKQKTVPELLDIAREMGIDNLARSRKQDIIFAILKKHAKSGEDIYGDGVLEILQDGFGFLRSADSSYLAGPDDIY
VSPSQIRRFNLRKGDSIAGKIRPPKEGERYFALLKVNEINFDRPENAKHKILFENLTPLFPQERMVMEIGNGSTEDLTSR
VIDLTSPIGKGQRGLIVSPPKAGKTLMLQNIATSITRNNPECHLIVLLIDERPEEVTEMSRTVRGEVVASTFDEPPARHV
QVAEMVIEKAKRLVEHKRDVVVLLDSITRLARAYNTVVPSSGKVLTGGVDAHALEKPKRFFGAARNIEEGGSLTIIATAL
VDTGSKMDEVIFEEFKGTGNMEAHLDRKLAEKRVYPALNIRRSGTRREDLIASEEEMQRMWILRKLLNPMDDVAATEFLI
DRLKDTKTNIEFFEAMKRR

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=78.7081339712919, Blast_Score=694, Evalue=0.0,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47179; Mature: 47179

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTELKQKTVPELLDIAREMGIDNLARSRKQDIIFAILKKHAKSGEDIYGDGVLEILQD
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRKGDSIAGKIRPPKEGERYFALLKVNEIN
HHHHHHCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
FDRPENAKHKILFENLTPLFPQERMVMEIGNGSTEDLTSRVIDLTSPIGKGQRGLIVSPP
CCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEECCC
KAGKTLMLQNIATSITRNNPECHLIVLLIDERPEEVTEMSRTVRGEVVASTFDEPPARHV
CCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
QVAEMVIEKAKRLVEHKRDVVVLLDSITRLARAYNTVVPSSGKVLTGGVDAHALEKPKRF
HHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
FGAARNIEEGGSLTIIATALVDTGSKMDEVIFEEFKGTGNMEAHLDRKLAEKRVYPALNI
HHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
RRSGTRREDLIASEEEMQRMWILRKLLNPMDDVAATEFLIDRLKDTKTNIEFFEAMKRR
CCCCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MNLTELKQKTVPELLDIAREMGIDNLARSRKQDIIFAILKKHAKSGEDIYGDGVLEILQD
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRKGDSIAGKIRPPKEGERYFALLKVNEIN
HHHHHHCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
FDRPENAKHKILFENLTPLFPQERMVMEIGNGSTEDLTSRVIDLTSPIGKGQRGLIVSPP
CCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEECCC
KAGKTLMLQNIATSITRNNPECHLIVLLIDERPEEVTEMSRTVRGEVVASTFDEPPARHV
CCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
QVAEMVIEKAKRLVEHKRDVVVLLDSITRLARAYNTVVPSSGKVLTGGVDAHALEKPKRF
HHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
FGAARNIEEGGSLTIIATALVDTGSKMDEVIFEEFKGTGNMEAHLDRKLAEKRVYPALNI
HHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
RRSGTRREDLIASEEEMQRMWILRKLLNPMDDVAATEFLIDRLKDTKTNIEFFEAMKRR
CCCCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]