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The map label for this gene is nemA [H]

Identifier: 92112641

GI number: 92112641

Start: 565522

End: 566619

Strand: Direct

Name: nemA [H]

Synonym: Csal_0508

Alternate gene names: 92112641

Gene position: 565522-566619 (Clockwise)

Preceding gene: 92112639

Following gene: 92112642

Centisome position: 15.3

GC content: 64.3

Gene sequence:

>1098_bases
ATGGCCTACGATCGGCTACTTTCCCCACTTACCATGGGCAAGCTGGAGCTGCCCAACCGTGTCCTGATGGCGCCACTGAC
ACGGGCGCGCACCCCGGACATGGTGCCCAAGGCGCTGCAGGCGACATATTACGCGCAGCGTGCCAATGCCGGCCTGATCA
TCAGCGAGGCAACGAACATCTCGCCGACCGCACGCGGCTATGTGTACACCCCGGGCATTTACACCGACGAGCAGGAAGCC
GGCTGGCGCGGGGTCGTGGACAGCGTGCACCGTGCCGGTGGACGGATTGCCCTGCAGCTCTGGCACGTGGGGCGCATCTC
GCACCACAAGATTCAGCCGGGCGGACAACCGCCGGTCGCGCCGAGCGCCTTGCGCGCCGAAGGGGCCAATTGCTTTCTTG
AGTTCGAGGACGGCAGCTCGGGGCAGCATCCCACCAGCACGCCTCGTGCGCTCGAGACCGAAGAAATCCCGGCTCTGATC
GATGATTATCGCCAGGCGGCCAAGCGTGCCAGGCGTGCAGGTTTCGACATGGTCGAAGTGCACGCCGCCAATGCGTACCT
GTTGCAACAGTTCATGGCGACCGGGTCGAACAAGCGTACCGACCGTTATGGCGGCAACCTGGTCAATCGGGCGCGCCTGG
TGCTGGAAGTCGTGGATGCCGTCTGCGAGGTGATGGGGGCCGACCGTGTCGGAATCCGTATCTCGCCGTTCATCGAGATT
TTCGGCCTGAGCGACGATGAATCGGAGGCGATGGCCTTCTATCTGGCCGAGCAACTGACGCGCCGCGGCATCGCCTACCT
GCATGTCAATGAGCCCGACTGGACCGGTGAAGGACCGCAACTGACCGACACCTTCCGCCGCGAGCTGCGCCAGCGCTTTC
CGGGCACCTTGATCTATTGCGGACACTACACCGCCGAGCGTGCCGAGGCGCTGATTCGCAATGGTCTGGGTGACGGTGCG
GCCTTCGGGCGCCCGTACATCGCCAACCCGGACCTCGTCGAACGCTTCCGCCGGGATAGCGCTCTCAACGAGCCCGATCC
GGCAACCTTCTACGGTGGCGGTGCCGAAGGGTACACGGACTACCCGACATTATCCTGA

Upstream 100 bases:

>100_bases
CGCAGTGTCATCAGCGTGTCACGCTCGGCCCCTTGATTAGTGCTCTAACGACCTCATCTAGTGCACATCACAGGGTTTCA
AGCGCTTACAAGGAGGCGAC

Downstream 100 bases:

>100_bases
GATCCAGGTGCGCAAAGGGTGTCCGAGGCTGGCGCATGACGCTATGCTGTCACCCACGGGTCGCATGAACGGCCCAGGAC
CCATTGGTTAGGAGGACTAT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MAYDRLLSPLTMGKLELPNRVLMAPLTRARTPDMVPKALQATYYAQRANAGLIISEATNISPTARGYVYTPGIYTDEQEA
GWRGVVDSVHRAGGRIALQLWHVGRISHHKIQPGGQPPVAPSALRAEGANCFLEFEDGSSGQHPTSTPRALETEEIPALI
DDYRQAAKRARRAGFDMVEVHAANAYLLQQFMATGSNKRTDRYGGNLVNRARLVLEVVDAVCEVMGADRVGIRISPFIEI
FGLSDDESEAMAFYLAEQLTRRGIAYLHVNEPDWTGEGPQLTDTFRRELRQRFPGTLIYCGHYTAERAEALIRNGLGDGA
AFGRPYIANPDLVERFRRDSALNEPDPATFYGGGAEGYTDYPTLS

Sequences:

>Translated_365_residues
MAYDRLLSPLTMGKLELPNRVLMAPLTRARTPDMVPKALQATYYAQRANAGLIISEATNISPTARGYVYTPGIYTDEQEA
GWRGVVDSVHRAGGRIALQLWHVGRISHHKIQPGGQPPVAPSALRAEGANCFLEFEDGSSGQHPTSTPRALETEEIPALI
DDYRQAAKRARRAGFDMVEVHAANAYLLQQFMATGSNKRTDRYGGNLVNRARLVLEVVDAVCEVMGADRVGIRISPFIEI
FGLSDDESEAMAFYLAEQLTRRGIAYLHVNEPDWTGEGPQLTDTFRRELRQRFPGTLIYCGHYTAERAEALIRNGLGDGA
AFGRPYIANPDLVERFRRDSALNEPDPATFYGGGAEGYTDYPTLS
>Mature_364_residues
AYDRLLSPLTMGKLELPNRVLMAPLTRARTPDMVPKALQATYYAQRANAGLIISEATNISPTARGYVYTPGIYTDEQEAG
WRGVVDSVHRAGGRIALQLWHVGRISHHKIQPGGQPPVAPSALRAEGANCFLEFEDGSSGQHPTSTPRALETEEIPALID
DYRQAAKRARRAGFDMVEVHAANAYLLQQFMATGSNKRTDRYGGNLVNRARLVLEVVDAVCEVMGADRVGIRISPFIEIF
GLSDDESEAMAFYLAEQLTRRGIAYLHVNEPDWTGEGPQLTDTFRRELRQRFPGTLIYCGHYTAERAEALIRNGLGDGAA
FGRPYIANPDLVERFRRDSALNEPDPATFYGGGAEGYTDYPTLS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=368, Percent_Identity=56.7934782608696, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1789463, Length=349, Percent_Identity=29.7994269340974, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=302, Percent_Identity=30.1324503311258, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=353, Percent_Identity=27.4787535410765, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=299, Percent_Identity=27.0903010033445, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=317, Percent_Identity=26.1829652996845, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=356, Percent_Identity=25, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=204, Percent_Identity=28.4313725490196, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=384, Percent_Identity=36.71875, Blast_Score=208, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6321973, Length=382, Percent_Identity=35.0785340314136, Blast_Score=195, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40278; Mature: 40147

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDRLLSPLTMGKLELPNRVLMAPLTRARTPDMVPKALQATYYAQRANAGLIISEATNI
CCHHHHHCCHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
SPTARGYVYTPGIYTDEQEAGWRGVVDSVHRAGGRIALQLWHVGRISHHKIQPGGQPPVA
CCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCC
PSALRAEGANCFLEFEDGSSGQHPTSTPRALETEEIPALIDDYRQAAKRARRAGFDMVEV
CHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
HAANAYLLQQFMATGSNKRTDRYGGNLVNRARLVLEVVDAVCEVMGADRVGIRISPFIEI
EHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECEEEE
FGLSDDESEAMAFYLAEQLTRRGIAYLHVNEPDWTGEGPQLTDTFRRELRQRFPGTLIYC
EECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
GHYTAERAEALIRNGLGDGAAFGRPYIANPDLVERFRRDSALNEPDPATFYGGGAEGYTD
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCC
YPTLS
CCCCC
>Mature Secondary Structure 
AYDRLLSPLTMGKLELPNRVLMAPLTRARTPDMVPKALQATYYAQRANAGLIISEATNI
CHHHHHCCHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
SPTARGYVYTPGIYTDEQEAGWRGVVDSVHRAGGRIALQLWHVGRISHHKIQPGGQPPVA
CCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCC
PSALRAEGANCFLEFEDGSSGQHPTSTPRALETEEIPALIDDYRQAAKRARRAGFDMVEV
CHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
HAANAYLLQQFMATGSNKRTDRYGGNLVNRARLVLEVVDAVCEVMGADRVGIRISPFIEI
EHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECEEEE
FGLSDDESEAMAFYLAEQLTRRGIAYLHVNEPDWTGEGPQLTDTFRRELRQRFPGTLIYC
EECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
GHYTAERAEALIRNGLGDGAAFGRPYIANPDLVERFRRDSALNEPDPATFYGGGAEGYTD
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCC
YPTLS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]